Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3791 and Pf1N1B4_3792 overlap by 4 nucleotides Pf1N1B4_3792 and Pf1N1B4_3793 are separated by 140 nucleotides Pf1N1B4_3793 and Pf1N1B4_3794 overlap by 2 nucleotides Pf1N1B4_3794 and Pf1N1B4_3795 are separated by 477 nucleotides
Pf1N1B4_3791 - Protein containing domains DUF403, at 4,199,431 to 4,200,381
_3791
Pf1N1B4_3792 - Protein containing transglutaminase-like domain, putative cysteine protease, at 4,200,378 to 4,201,187
_3792
Pf1N1B4_3793 - COG2010: Cytochrome c, mono- and diheme variants, at 4,201,328 to 4,202,149
_3793
Pf1N1B4_3794 - hypothetical protein, at 4,202,148 to 4,202,318
_3794
Pf1N1B4_3795 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 4,202,796 to 4,204,001
_3795
Position (kb)
4201
4202
4203 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4200.353 kb on + strand at 4200.354 kb on - strand at 4200.408 kb on + strand at 4200.408 kb on + strand at 4200.408 kb on + strand at 4200.408 kb on + strand at 4200.408 kb on + strand at 4200.409 kb on - strand at 4200.409 kb on - strand at 4200.409 kb on - strand at 4200.409 kb on - strand at 4200.409 kb on - strand at 4200.409 kb on - strand at 4200.409 kb on - strand at 4200.445 kb on - strand at 4200.746 kb on + strand, within Pf1N1B4_3792 at 4200.792 kb on + strand, within Pf1N1B4_3792 at 4200.793 kb on - strand, within Pf1N1B4_3792 at 4200.793 kb on - strand, within Pf1N1B4_3792 at 4200.814 kb on + strand, within Pf1N1B4_3792 at 4200.814 kb on + strand, within Pf1N1B4_3792 at 4200.815 kb on - strand, within Pf1N1B4_3792 at 4200.815 kb on - strand, within Pf1N1B4_3792 at 4200.815 kb on - strand, within Pf1N1B4_3792 at 4200.815 kb on - strand, within Pf1N1B4_3792 at 4200.918 kb on + strand, within Pf1N1B4_3792 at 4200.930 kb on + strand, within Pf1N1B4_3792 at 4200.936 kb on + strand, within Pf1N1B4_3792 at 4200.936 kb on + strand, within Pf1N1B4_3792 at 4200.936 kb on + strand, within Pf1N1B4_3792 at 4200.936 kb on + strand, within Pf1N1B4_3792 at 4200.937 kb on - strand, within Pf1N1B4_3792 at 4200.937 kb on - strand, within Pf1N1B4_3792 at 4200.937 kb on - strand, within Pf1N1B4_3792 at 4200.937 kb on - strand, within Pf1N1B4_3792 at 4200.937 kb on - strand, within Pf1N1B4_3792 at 4200.937 kb on - strand, within Pf1N1B4_3792 at 4201.003 kb on - strand, within Pf1N1B4_3792 at 4201.072 kb on - strand at 4201.185 kb on + strand at 4201.190 kb on + strand at 4201.191 kb on - strand at 4201.191 kb on - strand at 4201.365 kb on + strand at 4201.366 kb on - strand at 4201.467 kb on + strand, within Pf1N1B4_3793 at 4201.467 kb on + strand, within Pf1N1B4_3793 at 4201.468 kb on - strand, within Pf1N1B4_3793 at 4201.468 kb on - strand, within Pf1N1B4_3793 at 4201.468 kb on - strand, within Pf1N1B4_3793 at 4201.494 kb on + strand, within Pf1N1B4_3793 at 4201.720 kb on + strand at 4201.720 kb on + strand at 4201.843 kb on + strand, within Pf1N1B4_3793 at 4201.956 kb on + strand, within Pf1N1B4_3793 at 4201.957 kb on - strand, within Pf1N1B4_3793 at 4202.216 kb on - strand, within Pf1N1B4_3794 at 4202.216 kb on - strand, within Pf1N1B4_3794 at 4202.290 kb on + strand, within Pf1N1B4_3794 at 4202.300 kb on + strand, within Pf1N1B4_3794 at 4202.300 kb on + strand, within Pf1N1B4_3794 at 4202.301 kb on - strand at 4202.301 kb on - strand, within Pf1N1B4_3794 at 4202.301 kb on - strand, within Pf1N1B4_3794 at 4202.416 kb on - strand at 4202.457 kb on - strand at 4202.464 kb on + strand at 4202.465 kb on - strand at 4202.465 kb on - strand at 4202.465 kb on - strand at 4202.492 kb on + strand at 4202.492 kb on + strand at 4202.514 kb on - strand at 4202.547 kb on - strand at 4202.576 kb on - strand at 4202.687 kb on + strand at 4202.687 kb on + strand at 4202.687 kb on + strand at 4202.687 kb on + strand at 4202.700 kb on + strand at 4202.700 kb on + strand at 4202.701 kb on - strand at 4202.812 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 4,200,353 + -1.4 4,200,354 - -0.0 4,200,408 + -0.0 4,200,408 + -0.0 4,200,408 + -0.0 4,200,408 + -0.0 4,200,408 + -2.2 4,200,409 - -0.0 4,200,409 - -0.2 4,200,409 - -0.0 4,200,409 - -4.0 4,200,409 - -0.0 4,200,409 - -0.0 4,200,409 - -1.7 4,200,445 - -1.4 4,200,746 + Pf1N1B4_3792 0.45 -1.2 4,200,792 + Pf1N1B4_3792 0.51 -0.0 4,200,793 - Pf1N1B4_3792 0.51 -0.0 4,200,793 - Pf1N1B4_3792 0.51 -0.0 4,200,814 + Pf1N1B4_3792 0.54 -0.0 4,200,814 + Pf1N1B4_3792 0.54 -2.9 4,200,815 - Pf1N1B4_3792 0.54 -2.7 4,200,815 - Pf1N1B4_3792 0.54 -0.0 4,200,815 - Pf1N1B4_3792 0.54 -0.0 4,200,815 - Pf1N1B4_3792 0.54 -0.0 4,200,918 + Pf1N1B4_3792 0.67 -1.1 4,200,930 + Pf1N1B4_3792 0.68 -0.0 4,200,936 + Pf1N1B4_3792 0.69 -0.0 4,200,936 + Pf1N1B4_3792 0.69 -5.3 4,200,936 + Pf1N1B4_3792 0.69 -0.0 4,200,936 + Pf1N1B4_3792 0.69 -0.9 4,200,937 - Pf1N1B4_3792 0.69 -1.4 4,200,937 - Pf1N1B4_3792 0.69 -1.5 4,200,937 - Pf1N1B4_3792 0.69 -0.0 4,200,937 - Pf1N1B4_3792 0.69 -1.3 4,200,937 - Pf1N1B4_3792 0.69 -3.5 4,200,937 - Pf1N1B4_3792 0.69 -0.0 4,201,003 - Pf1N1B4_3792 0.77 -1.3 4,201,072 - -0.0 4,201,185 + -2.4 4,201,190 + -0.3 4,201,191 - -0.0 4,201,191 - -1.1 4,201,365 + -1.8 4,201,366 - -1.0 4,201,467 + Pf1N1B4_3793 0.17 -0.0 4,201,467 + Pf1N1B4_3793 0.17 -0.0 4,201,468 - Pf1N1B4_3793 0.17 -0.0 4,201,468 - Pf1N1B4_3793 0.17 -1.8 4,201,468 - Pf1N1B4_3793 0.17 +1.0 4,201,494 + Pf1N1B4_3793 0.20 -2.6 4,201,720 + -1.1 4,201,720 + -0.0 4,201,843 + Pf1N1B4_3793 0.63 -2.4 4,201,956 + Pf1N1B4_3793 0.76 -2.0 4,201,957 - Pf1N1B4_3793 0.77 -0.0 4,202,216 - Pf1N1B4_3794 0.40 -1.2 4,202,216 - Pf1N1B4_3794 0.40 -1.8 4,202,290 + Pf1N1B4_3794 0.83 -0.0 4,202,300 + Pf1N1B4_3794 0.89 -2.0 4,202,300 + Pf1N1B4_3794 0.89 -0.0 4,202,301 - -0.0 4,202,301 - Pf1N1B4_3794 0.89 -0.0 4,202,301 - Pf1N1B4_3794 0.89 -4.1 4,202,416 - -0.0 4,202,457 - -2.1 4,202,464 + -0.0 4,202,465 - -2.0 4,202,465 - -0.5 4,202,465 - -0.0 4,202,492 + -0.0 4,202,492 + -1.5 4,202,514 - -1.4 4,202,547 - -0.7 4,202,576 - -2.3 4,202,687 + -3.8 4,202,687 + -0.0 4,202,687 + -0.0 4,202,687 + -3.8 4,202,700 + -0.0 4,202,700 + -0.0 4,202,701 - -0.0 4,202,812 + -2.3
Or see this region's nucleotide sequence