Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf1N1B4_3622 and Pf1N1B4_3623 are separated by 11 nucleotides Pf1N1B4_3623 and Pf1N1B4_3624 overlap by 34 nucleotides Pf1N1B4_3624 and Pf1N1B4_3625 are separated by 233 nucleotides Pf1N1B4_3625 and Pf1N1B4_3626 overlap by 4 nucleotides  
        
        Pf1N1B4_3622 - Flagellar biosynthesis protein FlhA, at 4,029,868 to 4,031,997 
         
        _3622 
         
        
        Pf1N1B4_3623 - Flagellar biosynthesis protein FlhF, at 4,032,009 to 4,032,446 
         
        _3623 
         
        
        Pf1N1B4_3624 - Flagellar biosynthesis protein FlhF, at 4,032,413 to 4,033,336 
         
        _3624 
         
        
        Pf1N1B4_3625 - Flagellar synthesis regulator FleN, at 4,033,570 to 4,034,283 
         
        _3625 
         
        
        Pf1N1B4_3626 - RNA polymerase sigma factor for flagellar operon, at 4,034,280 to 4,035,020 
         
        _3626 
         Position (kb)  
4032 
 
4033 
 
4034 Strain fitness (log2 ratio)  
-3 
 
-2 
 
-1 
 
0 
 
1 at 4031.604 kb on + strand, within Pf1N1B4_3622 at 4031.666 kb on + strand, within Pf1N1B4_3622 at 4031.666 kb on + strand, within Pf1N1B4_3622 at 4031.667 kb on - strand at 4031.667 kb on - strand, within Pf1N1B4_3622 at 4031.720 kb on + strand, within Pf1N1B4_3622 at 4031.721 kb on - strand at 4031.730 kb on + strand at 4032.009 kb on - strand at 4032.017 kb on + strand at 4032.144 kb on + strand, within Pf1N1B4_3623 at 4032.145 kb on - strand, within Pf1N1B4_3623 at 4032.340 kb on - strand, within Pf1N1B4_3623 at 4032.340 kb on - strand, within Pf1N1B4_3623 at 4032.744 kb on - strand, within Pf1N1B4_3624 at 4032.755 kb on + strand, within Pf1N1B4_3624 at 4033.047 kb on + strand, within Pf1N1B4_3624 at 4033.048 kb on - strand, within Pf1N1B4_3624 at 4033.048 kb on - strand, within Pf1N1B4_3624 at 4033.067 kb on + strand, within Pf1N1B4_3624 at 4033.067 kb on + strand, within Pf1N1B4_3624 at 4033.067 kb on + strand, within Pf1N1B4_3624 at 4033.067 kb on + strand, within Pf1N1B4_3624 at 4033.068 kb on - strand, within Pf1N1B4_3624 at 4033.068 kb on - strand, within Pf1N1B4_3624 at 4033.102 kb on + strand, within Pf1N1B4_3624 at 4033.103 kb on - strand, within Pf1N1B4_3624 at 4033.172 kb on + strand, within Pf1N1B4_3624 at 4033.173 kb on - strand, within Pf1N1B4_3624 at 4033.251 kb on + strand at 4033.251 kb on + strand at 4033.251 kb on + strand at 4033.307 kb on + strand at 4033.307 kb on + strand at 4033.340 kb on - strand at 4033.340 kb on - strand at 4033.342 kb on + strand at 4033.342 kb on + strand at 4033.342 kb on + strand at 4033.343 kb on - strand at 4033.358 kb on + strand at 4033.358 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.363 kb on - strand at 4033.363 kb on - strand at 4033.363 kb on - strand at 4033.428 kb on - strand at 4033.431 kb on + strand at 4033.431 kb on + strand at 4033.431 kb on + strand at 4033.431 kb on + strand at 4033.432 kb on - strand at 4033.432 kb on - strand at 4033.432 kb on - strand at 4033.449 kb on + strand at 4033.449 kb on + strand at 4033.679 kb on + strand, within Pf1N1B4_3625 at 4033.679 kb on + strand, within Pf1N1B4_3625 at 4033.679 kb on + strand at 4033.680 kb on - strand at 4033.811 kb on + strand, within Pf1N1B4_3625 at 4033.811 kb on + strand, within Pf1N1B4_3625 at 4033.811 kb on + strand, within Pf1N1B4_3625 at 4034.066 kb on + strand, within Pf1N1B4_3625 at 4034.067 kb on - strand at 4034.067 kb on - strand, within Pf1N1B4_3625 at 4034.071 kb on + strand, within Pf1N1B4_3625 at 4034.134 kb on + strand, within Pf1N1B4_3625 at 4034.196 kb on + strand, within Pf1N1B4_3625 at 4034.279 kb on + strand at 4034.279 kb on + strand at 4034.279 kb on + strand at 4034.279 kb on + strand at 4034.279 kb on + strand at 4034.280 kb on - strand at 4034.296 kb on - strand at 4034.296 kb on - strand at 4034.296 kb on - strand at 4034.304 kb on + strand at 4034.305 kb on - strand at 4034.305 kb on - strand  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A             remove   4,031,604   +    Pf1N1B4_3622   0.82  -0.0   4,031,666   +    Pf1N1B4_3622   0.84  -0.0   4,031,666   +    Pf1N1B4_3622   0.84  -0.0   4,031,667   -        -0.0   4,031,667   -    Pf1N1B4_3622   0.84  -0.0   4,031,720   +    Pf1N1B4_3622   0.87  -0.0   4,031,721   -        -0.0   4,031,730   +        -0.0   4,032,009   -        -0.0   4,032,017   +        -0.0   4,032,144   +    Pf1N1B4_3623   0.31  -0.0   4,032,145   -    Pf1N1B4_3623   0.31  +1.0   4,032,340   -    Pf1N1B4_3623   0.76  -0.0   4,032,340   -    Pf1N1B4_3623   0.76  -0.0   4,032,744   -    Pf1N1B4_3624   0.36  -1.6   4,032,755   +    Pf1N1B4_3624   0.37  -1.7   4,033,047   +    Pf1N1B4_3624   0.69  -1.3   4,033,048   -    Pf1N1B4_3624   0.69  -1.5   4,033,048   -    Pf1N1B4_3624   0.69  -0.0   4,033,067   +    Pf1N1B4_3624   0.71  -0.0   4,033,067   +    Pf1N1B4_3624   0.71  -0.0   4,033,067   +    Pf1N1B4_3624   0.71  -0.0   4,033,067   +    Pf1N1B4_3624   0.71  -0.0   4,033,068   -    Pf1N1B4_3624   0.71  -0.0   4,033,068   -    Pf1N1B4_3624   0.71  -1.3   4,033,102   +    Pf1N1B4_3624   0.75  -0.0   4,033,103   -    Pf1N1B4_3624   0.75  -0.0   4,033,172   +    Pf1N1B4_3624   0.82  -0.0   4,033,173   -    Pf1N1B4_3624   0.82  -0.0   4,033,251   +        -0.0   4,033,251   +        -0.0   4,033,251   +        -0.0   4,033,307   +        -0.0   4,033,307   +        -0.0   4,033,340   -        -1.0   4,033,340   -        -0.0   4,033,342   +        -1.0   4,033,342   +        -2.5   4,033,342   +        -0.0   4,033,343   -        -0.1   4,033,358   +        -1.2   4,033,358   +        -0.0   4,033,362   +        -1.5   4,033,362   +        +1.1   4,033,362   +        -1.0   4,033,362   +        -0.9   4,033,362   +        -0.0   4,033,362   +        -0.0   4,033,362   +        -0.0   4,033,362   +        +0.1   4,033,363   -        -0.0   4,033,363   -        -0.0   4,033,363   -        -0.0   4,033,428   -        -0.0   4,033,431   +        -0.0   4,033,431   +        -0.7   4,033,431   +        -1.8   4,033,431   +        -0.0   4,033,432   -        -0.0   4,033,432   -        -0.0   4,033,432   -        +1.6   4,033,449   +        -0.0   4,033,449   +        -0.0   4,033,679   +    Pf1N1B4_3625   0.15  -0.0   4,033,679   +    Pf1N1B4_3625   0.15  -2.6   4,033,679   +        -0.0   4,033,680   -        -0.7   4,033,811   +    Pf1N1B4_3625   0.34  -0.0   4,033,811   +    Pf1N1B4_3625   0.34  -0.0   4,033,811   +    Pf1N1B4_3625   0.34  -0.0   4,034,066   +    Pf1N1B4_3625   0.69  -1.5   4,034,067   -        -0.0   4,034,067   -    Pf1N1B4_3625   0.70  -0.0   4,034,071   +    Pf1N1B4_3625   0.70  -0.0   4,034,134   +    Pf1N1B4_3625   0.79  -2.2   4,034,196   +    Pf1N1B4_3625   0.88  -0.0   4,034,279   +        -0.0   4,034,279   +        -1.9   4,034,279   +        -0.0   4,034,279   +        -0.0   4,034,279   +        -0.0   4,034,280   -        -0.0   4,034,296   -        -0.3   4,034,296   -        -1.7   4,034,296   -        -0.0   4,034,304   +        -3.1   4,034,305   -        -0.0   4,034,305   -        -1.5 
 
Or see this region's nucleotide sequence