Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3578 and Pf1N1B4_3579 overlap by 4 nucleotides Pf1N1B4_3579 and Pf1N1B4_3580 overlap by 4 nucleotides Pf1N1B4_3580 and Pf1N1B4_3581 overlap by 4 nucleotides Pf1N1B4_3581 and Pf1N1B4_3582 overlap by 11 nucleotides
Pf1N1B4_3578 - Similar to CDP-glucose 4,6-dehydratase (EC 4.2.1.45), at 3,988,897 to 3,989,979
_3578
Pf1N1B4_3579 - dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13), at 3,989,976 to 3,990,536
_3579
Pf1N1B4_3580 - methyltransferase, putative, at 3,990,533 to 3,991,759
_3580
Pf1N1B4_3581 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 3,991,756 to 3,992,613
_3581
Pf1N1B4_3582 - C-methyltransferase, at 3,992,603 to 3,993,673
_3582
Position (kb)
3990
3991
3992 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3989.576 kb on + strand, within Pf1N1B4_3578 at 3989.577 kb on - strand, within Pf1N1B4_3578 at 3989.595 kb on - strand, within Pf1N1B4_3578 at 3990.207 kb on - strand, within Pf1N1B4_3579 at 3990.378 kb on + strand, within Pf1N1B4_3579 at 3990.396 kb on + strand, within Pf1N1B4_3579 at 3990.532 kb on + strand at 3990.532 kb on + strand at 3990.608 kb on + strand at 3990.609 kb on - strand at 3990.609 kb on - strand at 3990.702 kb on - strand, within Pf1N1B4_3580 at 3990.786 kb on - strand, within Pf1N1B4_3580 at 3990.873 kb on - strand, within Pf1N1B4_3580 at 3990.873 kb on - strand, within Pf1N1B4_3580 at 3991.190 kb on + strand, within Pf1N1B4_3580 at 3991.190 kb on + strand, within Pf1N1B4_3580 at 3991.190 kb on + strand, within Pf1N1B4_3580 at 3991.190 kb on + strand, within Pf1N1B4_3580 at 3991.191 kb on - strand, within Pf1N1B4_3580 at 3991.417 kb on + strand, within Pf1N1B4_3580 at 3991.417 kb on + strand, within Pf1N1B4_3580 at 3991.417 kb on + strand, within Pf1N1B4_3580 at 3991.418 kb on - strand, within Pf1N1B4_3580 at 3991.517 kb on + strand, within Pf1N1B4_3580 at 3991.583 kb on + strand, within Pf1N1B4_3580 at 3991.583 kb on + strand, within Pf1N1B4_3580 at 3991.583 kb on + strand, within Pf1N1B4_3580 at 3991.583 kb on + strand, within Pf1N1B4_3580 at 3991.584 kb on - strand, within Pf1N1B4_3580 at 3991.584 kb on - strand, within Pf1N1B4_3580 at 3991.646 kb on + strand at 3991.646 kb on + strand at 3991.647 kb on - strand at 3991.647 kb on - strand at 3991.647 kb on - strand at 3991.647 kb on - strand at 3991.647 kb on - strand at 3991.704 kb on + strand at 3991.705 kb on - strand at 3991.911 kb on + strand, within Pf1N1B4_3581 at 3991.996 kb on + strand, within Pf1N1B4_3581 at 3991.996 kb on + strand, within Pf1N1B4_3581 at 3991.997 kb on - strand, within Pf1N1B4_3581 at 3992.022 kb on + strand, within Pf1N1B4_3581 at 3992.022 kb on + strand, within Pf1N1B4_3581 at 3992.023 kb on - strand, within Pf1N1B4_3581 at 3992.251 kb on + strand, within Pf1N1B4_3581 at 3992.588 kb on + strand at 3992.589 kb on - strand at 3992.589 kb on - strand at 3992.589 kb on - strand at 3992.618 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 3,989,576 + Pf1N1B4_3578 0.63 -0.0 3,989,577 - Pf1N1B4_3578 0.63 -0.0 3,989,595 - Pf1N1B4_3578 0.64 -1.8 3,990,207 - Pf1N1B4_3579 0.41 -2.6 3,990,378 + Pf1N1B4_3579 0.72 -0.0 3,990,396 + Pf1N1B4_3579 0.75 -5.4 3,990,532 + -2.3 3,990,532 + -0.0 3,990,608 + -0.0 3,990,609 - -0.0 3,990,609 - -0.0 3,990,702 - Pf1N1B4_3580 0.14 -2.8 3,990,786 - Pf1N1B4_3580 0.21 -1.6 3,990,873 - Pf1N1B4_3580 0.28 -0.0 3,990,873 - Pf1N1B4_3580 0.28 -1.0 3,991,190 + Pf1N1B4_3580 0.54 -1.9 3,991,190 + Pf1N1B4_3580 0.54 -0.0 3,991,190 + Pf1N1B4_3580 0.54 -1.6 3,991,190 + Pf1N1B4_3580 0.54 -0.0 3,991,191 - Pf1N1B4_3580 0.54 -0.0 3,991,417 + Pf1N1B4_3580 0.72 -0.0 3,991,417 + Pf1N1B4_3580 0.72 -0.0 3,991,417 + Pf1N1B4_3580 0.72 -2.9 3,991,418 - Pf1N1B4_3580 0.72 -0.0 3,991,517 + Pf1N1B4_3580 0.80 -2.5 3,991,583 + Pf1N1B4_3580 0.86 -0.0 3,991,583 + Pf1N1B4_3580 0.86 -0.0 3,991,583 + Pf1N1B4_3580 0.86 -2.6 3,991,583 + Pf1N1B4_3580 0.86 -0.0 3,991,584 - Pf1N1B4_3580 0.86 -0.0 3,991,584 - Pf1N1B4_3580 0.86 -0.0 3,991,646 + -1.9 3,991,646 + -0.0 3,991,647 - -2.2 3,991,647 - -0.0 3,991,647 - -1.2 3,991,647 - -2.2 3,991,647 - -0.0 3,991,704 + -2.1 3,991,705 - -0.0 3,991,911 + Pf1N1B4_3581 0.18 -1.3 3,991,996 + Pf1N1B4_3581 0.28 -0.0 3,991,996 + Pf1N1B4_3581 0.28 -3.4 3,991,997 - Pf1N1B4_3581 0.28 -0.0 3,992,022 + Pf1N1B4_3581 0.31 -0.0 3,992,022 + Pf1N1B4_3581 0.31 -0.0 3,992,023 - Pf1N1B4_3581 0.31 -0.0 3,992,251 + Pf1N1B4_3581 0.58 -0.0 3,992,588 + -0.0 3,992,589 - -0.0 3,992,589 - -0.0 3,992,589 - -0.0 3,992,618 + -0.0
Or see this region's nucleotide sequence