Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3580

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3578 and Pf1N1B4_3579 overlap by 4 nucleotidesPf1N1B4_3579 and Pf1N1B4_3580 overlap by 4 nucleotidesPf1N1B4_3580 and Pf1N1B4_3581 overlap by 4 nucleotidesPf1N1B4_3581 and Pf1N1B4_3582 overlap by 11 nucleotides Pf1N1B4_3578 - Similar to CDP-glucose 4,6-dehydratase (EC 4.2.1.45), at 3,988,897 to 3,989,979 _3578 Pf1N1B4_3579 - dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13), at 3,989,976 to 3,990,536 _3579 Pf1N1B4_3580 - methyltransferase, putative, at 3,990,533 to 3,991,759 _3580 Pf1N1B4_3581 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 3,991,756 to 3,992,613 _3581 Pf1N1B4_3582 - C-methyltransferase, at 3,992,603 to 3,993,673 _3582 Position (kb) 3990 3991 3992Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3989.576 kb on + strand, within Pf1N1B4_3578at 3989.577 kb on - strand, within Pf1N1B4_3578at 3989.595 kb on - strand, within Pf1N1B4_3578at 3990.207 kb on - strand, within Pf1N1B4_3579at 3990.378 kb on + strand, within Pf1N1B4_3579at 3990.396 kb on + strand, within Pf1N1B4_3579at 3990.532 kb on + strandat 3990.532 kb on + strandat 3990.608 kb on + strandat 3990.609 kb on - strandat 3990.609 kb on - strandat 3990.702 kb on - strand, within Pf1N1B4_3580at 3990.786 kb on - strand, within Pf1N1B4_3580at 3990.873 kb on - strand, within Pf1N1B4_3580at 3990.873 kb on - strand, within Pf1N1B4_3580at 3991.190 kb on + strand, within Pf1N1B4_3580at 3991.190 kb on + strand, within Pf1N1B4_3580at 3991.190 kb on + strand, within Pf1N1B4_3580at 3991.190 kb on + strand, within Pf1N1B4_3580at 3991.191 kb on - strand, within Pf1N1B4_3580at 3991.417 kb on + strand, within Pf1N1B4_3580at 3991.417 kb on + strand, within Pf1N1B4_3580at 3991.417 kb on + strand, within Pf1N1B4_3580at 3991.418 kb on - strand, within Pf1N1B4_3580at 3991.517 kb on + strand, within Pf1N1B4_3580at 3991.583 kb on + strand, within Pf1N1B4_3580at 3991.583 kb on + strand, within Pf1N1B4_3580at 3991.583 kb on + strand, within Pf1N1B4_3580at 3991.583 kb on + strand, within Pf1N1B4_3580at 3991.584 kb on - strand, within Pf1N1B4_3580at 3991.584 kb on - strand, within Pf1N1B4_3580at 3991.646 kb on + strandat 3991.646 kb on + strandat 3991.647 kb on - strandat 3991.647 kb on - strandat 3991.647 kb on - strandat 3991.647 kb on - strandat 3991.647 kb on - strandat 3991.704 kb on + strandat 3991.705 kb on - strandat 3991.911 kb on + strand, within Pf1N1B4_3581at 3991.996 kb on + strand, within Pf1N1B4_3581at 3991.996 kb on + strand, within Pf1N1B4_3581at 3991.997 kb on - strand, within Pf1N1B4_3581at 3992.022 kb on + strand, within Pf1N1B4_3581at 3992.022 kb on + strand, within Pf1N1B4_3581at 3992.023 kb on - strand, within Pf1N1B4_3581at 3992.251 kb on + strand, within Pf1N1B4_3581at 3992.588 kb on + strandat 3992.589 kb on - strandat 3992.589 kb on - strandat 3992.589 kb on - strandat 3992.618 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
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3,989,576 + Pf1N1B4_3578 0.63 -0.0
3,989,577 - Pf1N1B4_3578 0.63 -0.0
3,989,595 - Pf1N1B4_3578 0.64 -1.8
3,990,207 - Pf1N1B4_3579 0.41 -2.6
3,990,378 + Pf1N1B4_3579 0.72 -0.0
3,990,396 + Pf1N1B4_3579 0.75 -5.4
3,990,532 + -2.3
3,990,532 + -0.0
3,990,608 + -0.0
3,990,609 - -0.0
3,990,609 - -0.0
3,990,702 - Pf1N1B4_3580 0.14 -2.8
3,990,786 - Pf1N1B4_3580 0.21 -1.6
3,990,873 - Pf1N1B4_3580 0.28 -0.0
3,990,873 - Pf1N1B4_3580 0.28 -1.0
3,991,190 + Pf1N1B4_3580 0.54 -1.9
3,991,190 + Pf1N1B4_3580 0.54 -0.0
3,991,190 + Pf1N1B4_3580 0.54 -1.6
3,991,190 + Pf1N1B4_3580 0.54 -0.0
3,991,191 - Pf1N1B4_3580 0.54 -0.0
3,991,417 + Pf1N1B4_3580 0.72 -0.0
3,991,417 + Pf1N1B4_3580 0.72 -0.0
3,991,417 + Pf1N1B4_3580 0.72 -2.9
3,991,418 - Pf1N1B4_3580 0.72 -0.0
3,991,517 + Pf1N1B4_3580 0.80 -2.5
3,991,583 + Pf1N1B4_3580 0.86 -0.0
3,991,583 + Pf1N1B4_3580 0.86 -0.0
3,991,583 + Pf1N1B4_3580 0.86 -2.6
3,991,583 + Pf1N1B4_3580 0.86 -0.0
3,991,584 - Pf1N1B4_3580 0.86 -0.0
3,991,584 - Pf1N1B4_3580 0.86 -0.0
3,991,646 + -1.9
3,991,646 + -0.0
3,991,647 - -2.2
3,991,647 - -0.0
3,991,647 - -1.2
3,991,647 - -2.2
3,991,647 - -0.0
3,991,704 + -2.1
3,991,705 - -0.0
3,991,911 + Pf1N1B4_3581 0.18 -1.3
3,991,996 + Pf1N1B4_3581 0.28 -0.0
3,991,996 + Pf1N1B4_3581 0.28 -3.4
3,991,997 - Pf1N1B4_3581 0.28 -0.0
3,992,022 + Pf1N1B4_3581 0.31 -0.0
3,992,022 + Pf1N1B4_3581 0.31 -0.0
3,992,023 - Pf1N1B4_3581 0.31 -0.0
3,992,251 + Pf1N1B4_3581 0.58 -0.0
3,992,588 + -0.0
3,992,589 - -0.0
3,992,589 - -0.0
3,992,589 - -0.0
3,992,618 + -0.0

Or see this region's nucleotide sequence