Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_356 and Pf1N1B4_357 are separated by 4 nucleotides Pf1N1B4_357 and Pf1N1B4_358 are separated by 186 nucleotides Pf1N1B4_358 and Pf1N1B4_359 are separated by 374 nucleotides
Pf1N1B4_356 - 3-demethylubiquinone-9 3-methyltransferase (EC 2.1.1.64), at 423,359 to 424,057
_356
Pf1N1B4_357 - Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase, at 424,062 to 424,733
_357
Pf1N1B4_358 - Oxidoreductase, short chain dehydrogenase/reductase family, at 424,920 to 425,660
_358
Pf1N1B4_359 - GGDEF domain protein, at 426,035 to 426,961
_359
Position (kb) 424
425
426 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 424.630 kb on - strand, within Pf1N1B4_357 at 424.793 kb on + strand at 424.793 kb on + strand at 424.793 kb on + strand at 424.794 kb on - strand at 424.794 kb on - strand at 424.794 kb on - strand at 424.794 kb on - strand at 424.797 kb on + strand at 424.797 kb on + strand at 424.797 kb on + strand at 424.797 kb on + strand at 424.797 kb on + strand at 424.797 kb on + strand at 424.797 kb on + strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.838 kb on - strand at 424.883 kb on + strand at 424.883 kb on + strand at 424.883 kb on + strand at 424.883 kb on + strand at 424.883 kb on + strand at 424.919 kb on + strand at 424.919 kb on + strand at 424.920 kb on - strand at 424.920 kb on - strand at 424.920 kb on - strand at 424.930 kb on - strand at 424.930 kb on - strand at 425.017 kb on - strand, within Pf1N1B4_358 at 425.017 kb on - strand at 425.079 kb on + strand, within Pf1N1B4_358 at 425.079 kb on + strand, within Pf1N1B4_358 at 425.079 kb on + strand, within Pf1N1B4_358 at 425.079 kb on + strand, within Pf1N1B4_358 at 425.080 kb on - strand, within Pf1N1B4_358 at 425.080 kb on - strand, within Pf1N1B4_358 at 425.080 kb on - strand, within Pf1N1B4_358 at 425.080 kb on - strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand at 425.157 kb on + strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.544 kb on + strand, within Pf1N1B4_358 at 425.598 kb on + strand at 425.598 kb on + strand at 425.598 kb on + strand at 425.599 kb on - strand at 425.599 kb on - strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.605 kb on - strand at 425.605 kb on - strand at 425.658 kb on + strand at 425.721 kb on - strand at 425.745 kb on - strand at 425.745 kb on - strand at 425.745 kb on - strand at 425.905 kb on + strand at 425.905 kb on + strand at 425.906 kb on - strand at 425.925 kb on + strand at 425.925 kb on + strand at 426.001 kb on + strand at 426.012 kb on + strand at 426.013 kb on - strand at 426.211 kb on - strand, within Pf1N1B4_359 at 426.317 kb on + strand, within Pf1N1B4_359 at 426.620 kb on + strand, within Pf1N1B4_359 at 426.620 kb on + strand, within Pf1N1B4_359 at 426.620 kb on + strand, within Pf1N1B4_359 at 426.621 kb on - strand, within Pf1N1B4_359
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 424,630 - Pf1N1B4_357 0.85 -1.8 424,793 + -0.0 424,793 + -0.8 424,793 + -0.0 424,794 - -0.0 424,794 - -2.8 424,794 - -0.0 424,794 - -2.9 424,797 + -0.0 424,797 + -0.0 424,797 + -0.0 424,797 + -0.0 424,797 + -2.8 424,797 + -0.0 424,797 + -1.8 424,798 - -2.3 424,798 - -3.3 424,798 - -0.0 424,798 - -0.0 424,798 - -0.0 424,798 - -0.0 424,798 - -0.0 424,798 - -0.0 424,798 - -1.2 424,798 - -0.0 424,798 - -0.0 424,798 - -1.3 424,798 - -0.0 424,838 - -0.0 424,883 + -1.4 424,883 + -2.4 424,883 + -0.0 424,883 + -0.0 424,883 + -0.0 424,919 + -0.0 424,919 + -2.8 424,920 - -0.0 424,920 - -0.3 424,920 - -0.0 424,930 - -0.0 424,930 - -1.0 425,017 - Pf1N1B4_358 0.13 -0.0 425,017 - -0.0 425,079 + Pf1N1B4_358 0.21 -0.0 425,079 + Pf1N1B4_358 0.21 -1.8 425,079 + Pf1N1B4_358 0.21 -4.7 425,079 + Pf1N1B4_358 0.21 -2.5 425,080 - Pf1N1B4_358 0.22 -0.0 425,080 - Pf1N1B4_358 0.22 -0.0 425,080 - Pf1N1B4_358 0.22 -0.0 425,080 - Pf1N1B4_358 0.22 -4.2 425,157 + Pf1N1B4_358 0.32 -1.0 425,157 + Pf1N1B4_358 0.32 -0.0 425,157 + Pf1N1B4_358 0.32 -0.0 425,157 + Pf1N1B4_358 0.32 -0.0 425,157 + Pf1N1B4_358 0.32 -0.0 425,157 + -0.0 425,157 + Pf1N1B4_358 0.32 -3.2 425,158 - Pf1N1B4_358 0.32 -0.0 425,158 - Pf1N1B4_358 0.32 -0.0 425,158 - Pf1N1B4_358 0.32 -0.0 425,158 - Pf1N1B4_358 0.32 -3.5 425,158 - Pf1N1B4_358 0.32 -0.0 425,470 + Pf1N1B4_358 0.74 -0.0 425,470 + Pf1N1B4_358 0.74 -2.5 425,470 + Pf1N1B4_358 0.74 -0.0 425,470 + Pf1N1B4_358 0.74 -2.2 425,470 + Pf1N1B4_358 0.74 -4.3 425,470 + Pf1N1B4_358 0.74 -3.0 425,471 - Pf1N1B4_358 0.74 -0.0 425,471 - Pf1N1B4_358 0.74 -0.0 425,471 - Pf1N1B4_358 0.74 -0.0 425,471 - Pf1N1B4_358 0.74 -0.0 425,471 - Pf1N1B4_358 0.74 -0.0 425,471 - Pf1N1B4_358 0.74 -0.0 425,471 - Pf1N1B4_358 0.74 -0.0 425,544 + Pf1N1B4_358 0.84 -0.0 425,598 + -0.0 425,598 + -0.0 425,598 + -2.8 425,599 - -0.0 425,599 - -3.2 425,604 + -0.0 425,604 + -1.0 425,604 + -0.0 425,604 + -0.0 425,604 + -3.0 425,604 + -0.0 425,605 - -0.0 425,605 - -3.0 425,658 + -0.0 425,721 - -2.3 425,745 - -2.0 425,745 - -0.0 425,745 - -0.8 425,905 + -0.0 425,905 + -0.5 425,906 - -0.8 425,925 + -0.0 425,925 + -1.6 426,001 + -0.0 426,012 + -1.5 426,013 - -1.7 426,211 - Pf1N1B4_359 0.19 -0.0 426,317 + Pf1N1B4_359 0.30 -0.9 426,620 + Pf1N1B4_359 0.63 -1.3 426,620 + Pf1N1B4_359 0.63 -0.0 426,620 + Pf1N1B4_359 0.63 -0.0 426,621 - Pf1N1B4_359 0.63 -1.3
Or see this region's nucleotide sequence