Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3448

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3447 and Pf1N1B4_3448 are separated by 112 nucleotidesPf1N1B4_3448 and Pf1N1B4_3449 are separated by 573 nucleotides Pf1N1B4_3447 - Succinylglutamate desuccinylase (EC 3.5.1.96), at 3,842,468 to 3,843,478 _3447 Pf1N1B4_3448 - 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78), at 3,843,591 to 3,844,100 _3448 Pf1N1B4_3449 - Low-specificity L-threonine aldolase (EC 4.1.2.48), at 3,844,674 to 3,845,678 _3449 Position (kb) 3843 3844 3845Strain fitness (log2 ratio) -3 -2 -1 0 1at 3842.909 kb on + strand, within Pf1N1B4_3447at 3842.960 kb on + strand, within Pf1N1B4_3447at 3843.046 kb on + strand, within Pf1N1B4_3447at 3843.071 kb on + strand, within Pf1N1B4_3447at 3843.071 kb on + strand, within Pf1N1B4_3447at 3843.071 kb on + strand, within Pf1N1B4_3447at 3843.071 kb on + strand, within Pf1N1B4_3447at 3843.071 kb on + strandat 3843.071 kb on + strandat 3843.071 kb on + strand, within Pf1N1B4_3447at 3843.072 kb on - strand, within Pf1N1B4_3447at 3843.072 kb on - strandat 3843.072 kb on - strand, within Pf1N1B4_3447at 3843.072 kb on - strand, within Pf1N1B4_3447at 3843.073 kb on + strand, within Pf1N1B4_3447at 3843.073 kb on + strand, within Pf1N1B4_3447at 3843.074 kb on - strand, within Pf1N1B4_3447at 3843.074 kb on - strand, within Pf1N1B4_3447at 3843.077 kb on + strand, within Pf1N1B4_3447at 3843.077 kb on + strand, within Pf1N1B4_3447at 3843.077 kb on + strand, within Pf1N1B4_3447at 3843.077 kb on + strandat 3843.078 kb on - strand, within Pf1N1B4_3447at 3843.078 kb on - strand, within Pf1N1B4_3447at 3843.078 kb on - strand, within Pf1N1B4_3447at 3843.078 kb on - strandat 3843.078 kb on - strand, within Pf1N1B4_3447at 3843.277 kb on + strand, within Pf1N1B4_3447at 3843.335 kb on + strand, within Pf1N1B4_3447at 3843.335 kb on + strandat 3843.335 kb on + strand, within Pf1N1B4_3447at 3843.336 kb on - strand, within Pf1N1B4_3447at 3843.336 kb on - strand, within Pf1N1B4_3447at 3843.336 kb on - strand, within Pf1N1B4_3447at 3843.476 kb on + strandat 3843.680 kb on + strandat 3843.680 kb on + strand, within Pf1N1B4_3448at 3843.681 kb on - strand, within Pf1N1B4_3448at 3843.681 kb on - strandat 3843.832 kb on + strand, within Pf1N1B4_3448at 3843.832 kb on + strand, within Pf1N1B4_3448at 3843.833 kb on - strand, within Pf1N1B4_3448at 3843.833 kb on - strand, within Pf1N1B4_3448at 3843.833 kb on - strand, within Pf1N1B4_3448at 3843.833 kb on - strand, within Pf1N1B4_3448at 3844.154 kb on - strandat 3844.159 kb on + strandat 3844.194 kb on + strandat 3844.194 kb on + strandat 3844.196 kb on + strandat 3844.196 kb on + strandat 3844.196 kb on + strandat 3844.239 kb on + strandat 3844.240 kb on - strandat 3844.240 kb on - strandat 3844.240 kb on - strandat 3844.240 kb on - strandat 3844.327 kb on + strandat 3844.417 kb on + strandat 3844.418 kb on - strandat 3844.424 kb on + strandat 3844.424 kb on + strandat 3844.424 kb on + strandat 3844.424 kb on + strandat 3844.425 kb on - strandat 3844.507 kb on + strandat 3844.507 kb on + strandat 3844.507 kb on + strandat 3844.507 kb on + strandat 3844.507 kb on + strandat 3844.507 kb on + strandat 3844.507 kb on + strandat 3844.508 kb on - strandat 3844.606 kb on + strandat 3844.627 kb on + strandat 3844.628 kb on - strandat 3844.767 kb on + strandat 3844.768 kb on - strandat 3844.768 kb on - strandat 3844.768 kb on - strandat 3844.768 kb on - strandat 3844.905 kb on + strand, within Pf1N1B4_3449at 3844.905 kb on + strand, within Pf1N1B4_3449at 3844.905 kb on + strand, within Pf1N1B4_3449at 3844.905 kb on + strand, within Pf1N1B4_3449at 3844.905 kb on + strand, within Pf1N1B4_3449at 3844.906 kb on - strand, within Pf1N1B4_3449at 3844.906 kb on - strand, within Pf1N1B4_3449at 3844.906 kb on - strand, within Pf1N1B4_3449at 3844.906 kb on - strand, within Pf1N1B4_3449at 3844.906 kb on - strand, within Pf1N1B4_3449at 3844.906 kb on - strand, within Pf1N1B4_3449at 3844.906 kb on - strand, within Pf1N1B4_3449at 3844.932 kb on + strand, within Pf1N1B4_3449at 3844.932 kb on + strand, within Pf1N1B4_3449at 3844.932 kb on + strandat 3844.932 kb on + strand, within Pf1N1B4_3449at 3844.932 kb on + strand, within Pf1N1B4_3449at 3844.932 kb on + strand, within Pf1N1B4_3449at 3844.932 kb on + strand, within Pf1N1B4_3449at 3844.933 kb on - strand, within Pf1N1B4_3449at 3844.933 kb on - strand, within Pf1N1B4_3449at 3844.933 kb on - strandat 3844.933 kb on - strand, within Pf1N1B4_3449at 3844.933 kb on - strand, within Pf1N1B4_3449at 3844.933 kb on - strand, within Pf1N1B4_3449at 3844.933 kb on - strand, within Pf1N1B4_3449at 3844.933 kb on - strand, within Pf1N1B4_3449at 3844.933 kb on - strand, within Pf1N1B4_3449at 3844.933 kb on - strand, within Pf1N1B4_3449at 3844.933 kb on - strand, within Pf1N1B4_3449at 3844.933 kb on - strand, within Pf1N1B4_3449at 3844.934 kb on + strand, within Pf1N1B4_3449at 3844.934 kb on + strand, within Pf1N1B4_3449at 3844.934 kb on + strand, within Pf1N1B4_3449at 3844.934 kb on + strand, within Pf1N1B4_3449at 3844.934 kb on + strand, within Pf1N1B4_3449at 3844.934 kb on + strand, within Pf1N1B4_3449at 3844.934 kb on + strand, within Pf1N1B4_3449at 3844.935 kb on - strand, within Pf1N1B4_3449at 3844.935 kb on - strand, within Pf1N1B4_3449at 3844.935 kb on - strand, within Pf1N1B4_3449at 3844.935 kb on - strand, within Pf1N1B4_3449at 3844.935 kb on - strand, within Pf1N1B4_3449at 3844.935 kb on - strand, within Pf1N1B4_3449at 3844.935 kb on - strand, within Pf1N1B4_3449at 3844.939 kb on - strandat 3844.939 kb on - strand, within Pf1N1B4_3449at 3844.939 kb on - strand, within Pf1N1B4_3449at 3844.939 kb on - strand, within Pf1N1B4_3449at 3844.939 kb on - strand, within Pf1N1B4_3449at 3844.939 kb on - strand, within Pf1N1B4_3449at 3844.939 kb on - strand, within Pf1N1B4_3449at 3844.939 kb on - strand, within Pf1N1B4_3449

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
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3,842,909 + Pf1N1B4_3447 0.44 -0.0
3,842,960 + Pf1N1B4_3447 0.49 -0.8
3,843,046 + Pf1N1B4_3447 0.57 -1.0
3,843,071 + Pf1N1B4_3447 0.60 -0.0
3,843,071 + Pf1N1B4_3447 0.60 -0.0
3,843,071 + Pf1N1B4_3447 0.60 -0.0
3,843,071 + Pf1N1B4_3447 0.60 -0.0
3,843,071 + -1.0
3,843,071 + -0.0
3,843,071 + Pf1N1B4_3447 0.60 -2.9
3,843,072 - Pf1N1B4_3447 0.60 -0.0
3,843,072 - -1.0
3,843,072 - Pf1N1B4_3447 0.60 -3.2
3,843,072 - Pf1N1B4_3447 0.60 -0.0
3,843,073 + Pf1N1B4_3447 0.60 -0.0
3,843,073 + Pf1N1B4_3447 0.60 -0.0
3,843,074 - Pf1N1B4_3447 0.60 -0.0
3,843,074 - Pf1N1B4_3447 0.60 -0.0
3,843,077 + Pf1N1B4_3447 0.60 -0.0
3,843,077 + Pf1N1B4_3447 0.60 -1.5
3,843,077 + Pf1N1B4_3447 0.60 -1.1
3,843,077 + -0.0
3,843,078 - Pf1N1B4_3447 0.60 -0.0
3,843,078 - Pf1N1B4_3447 0.60 -0.0
3,843,078 - Pf1N1B4_3447 0.60 -2.6
3,843,078 - -0.0
3,843,078 - Pf1N1B4_3447 0.60 -0.0
3,843,277 + Pf1N1B4_3447 0.80 -0.0
3,843,335 + Pf1N1B4_3447 0.86 -0.0
3,843,335 + -1.6
3,843,335 + Pf1N1B4_3447 0.86 -1.9
3,843,336 - Pf1N1B4_3447 0.86 -0.0
3,843,336 - Pf1N1B4_3447 0.86 -0.0
3,843,336 - Pf1N1B4_3447 0.86 -0.0
3,843,476 + -0.0
3,843,680 + -0.0
3,843,680 + Pf1N1B4_3448 0.17 -0.0
3,843,681 - Pf1N1B4_3448 0.18 -2.5
3,843,681 - -0.0
3,843,832 + Pf1N1B4_3448 0.47 -2.4
3,843,832 + Pf1N1B4_3448 0.47 -1.9
3,843,833 - Pf1N1B4_3448 0.47 -0.0
3,843,833 - Pf1N1B4_3448 0.47 -0.0
3,843,833 - Pf1N1B4_3448 0.47 -0.0
3,843,833 - Pf1N1B4_3448 0.47 -0.0
3,844,154 - -1.2
3,844,159 + -0.0
3,844,194 + -1.5
3,844,194 + -0.0
3,844,196 + -2.0
3,844,196 + -2.1
3,844,196 + -0.0
3,844,239 + -2.0
3,844,240 - -0.0
3,844,240 - -2.4
3,844,240 - -0.0
3,844,240 - -0.0
3,844,327 + -0.0
3,844,417 + -0.0
3,844,418 - -0.0
3,844,424 + -2.7
3,844,424 + -0.0
3,844,424 + -2.2
3,844,424 + -1.0
3,844,425 - -0.7
3,844,507 + -0.0
3,844,507 + -0.0
3,844,507 + -0.0
3,844,507 + -1.4
3,844,507 + -0.0
3,844,507 + -0.0
3,844,507 + -1.7
3,844,508 - -0.0
3,844,606 + -0.0
3,844,627 + -2.0
3,844,628 - -0.0
3,844,767 + -0.0
3,844,768 - -3.3
3,844,768 - -0.6
3,844,768 - -0.0
3,844,768 - -3.2
3,844,905 + Pf1N1B4_3449 0.23 -0.0
3,844,905 + Pf1N1B4_3449 0.23 -2.5
3,844,905 + Pf1N1B4_3449 0.23 -0.0
3,844,905 + Pf1N1B4_3449 0.23 -3.1
3,844,905 + Pf1N1B4_3449 0.23 -1.0
3,844,906 - Pf1N1B4_3449 0.23 +1.0
3,844,906 - Pf1N1B4_3449 0.23 -1.3
3,844,906 - Pf1N1B4_3449 0.23 -0.0
3,844,906 - Pf1N1B4_3449 0.23 -0.0
3,844,906 - Pf1N1B4_3449 0.23 -0.0
3,844,906 - Pf1N1B4_3449 0.23 -0.0
3,844,906 - Pf1N1B4_3449 0.23 -1.2
3,844,932 + Pf1N1B4_3449 0.26 -1.5
3,844,932 + Pf1N1B4_3449 0.26 -1.7
3,844,932 + -0.0
3,844,932 + Pf1N1B4_3449 0.26 -0.0
3,844,932 + Pf1N1B4_3449 0.26 -0.0
3,844,932 + Pf1N1B4_3449 0.26 -0.0
3,844,932 + Pf1N1B4_3449 0.26 -0.0
3,844,933 - Pf1N1B4_3449 0.26 -0.0
3,844,933 - Pf1N1B4_3449 0.26 -0.0
3,844,933 - -0.0
3,844,933 - Pf1N1B4_3449 0.26 -3.1
3,844,933 - Pf1N1B4_3449 0.26 -0.0
3,844,933 - Pf1N1B4_3449 0.26 -1.0
3,844,933 - Pf1N1B4_3449 0.26 -0.0
3,844,933 - Pf1N1B4_3449 0.26 -1.1
3,844,933 - Pf1N1B4_3449 0.26 -0.0
3,844,933 - Pf1N1B4_3449 0.26 -0.0
3,844,933 - Pf1N1B4_3449 0.26 -0.0
3,844,933 - Pf1N1B4_3449 0.26 -0.0
3,844,934 + Pf1N1B4_3449 0.26 -0.0
3,844,934 + Pf1N1B4_3449 0.26 -0.0
3,844,934 + Pf1N1B4_3449 0.26 -0.0
3,844,934 + Pf1N1B4_3449 0.26 -2.3
3,844,934 + Pf1N1B4_3449 0.26 -1.7
3,844,934 + Pf1N1B4_3449 0.26 -0.0
3,844,934 + Pf1N1B4_3449 0.26 -0.0
3,844,935 - Pf1N1B4_3449 0.26 -0.0
3,844,935 - Pf1N1B4_3449 0.26 -0.0
3,844,935 - Pf1N1B4_3449 0.26 -0.0
3,844,935 - Pf1N1B4_3449 0.26 -0.0
3,844,935 - Pf1N1B4_3449 0.26 -0.0
3,844,935 - Pf1N1B4_3449 0.26 -0.0
3,844,935 - Pf1N1B4_3449 0.26 -0.0
3,844,939 - -0.0
3,844,939 - Pf1N1B4_3449 0.26 -0.0
3,844,939 - Pf1N1B4_3449 0.26 -0.0
3,844,939 - Pf1N1B4_3449 0.26 -0.0
3,844,939 - Pf1N1B4_3449 0.26 -1.8
3,844,939 - Pf1N1B4_3449 0.26 -0.0
3,844,939 - Pf1N1B4_3449 0.26 -0.0
3,844,939 - Pf1N1B4_3449 0.26 -0.0

Or see this region's nucleotide sequence