Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3444 and Pf1N1B4_3445 are separated by 161 nucleotides Pf1N1B4_3445 and Pf1N1B4_3446 are separated by 19 nucleotides Pf1N1B4_3446 and Pf1N1B4_3447 are separated by 12 nucleotides
Pf1N1B4_3444 - Succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71), at 3,839,171 to 3,840,637
_3444
Pf1N1B4_3445 - Succinylarginine dihydrolase (EC 3.5.3.23), at 3,840,799 to 3,842,145
_3445
Pf1N1B4_3446 - Putative GTPases (G3E family), at 3,842,165 to 3,842,455
_3446
Pf1N1B4_3447 - Succinylglutamate desuccinylase (EC 3.5.1.96), at 3,842,468 to 3,843,478
_3447
Position (kb)
3840
3841
3842
3843 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3840.539 kb on + strand at 3840.539 kb on + strand at 3840.540 kb on - strand at 3840.542 kb on + strand at 3840.542 kb on + strand at 3840.554 kb on + strand at 3840.680 kb on - strand at 3840.748 kb on + strand at 3840.756 kb on - strand at 3840.759 kb on + strand at 3840.759 kb on + strand at 3840.851 kb on - strand at 3841.244 kb on + strand, within Pf1N1B4_3445 at 3841.354 kb on + strand, within Pf1N1B4_3445 at 3841.504 kb on + strand, within Pf1N1B4_3445 at 3841.505 kb on - strand, within Pf1N1B4_3445 at 3841.589 kb on - strand at 3841.589 kb on - strand, within Pf1N1B4_3445 at 3841.723 kb on + strand, within Pf1N1B4_3445 at 3842.173 kb on + strand at 3842.304 kb on + strand, within Pf1N1B4_3446 at 3842.305 kb on - strand, within Pf1N1B4_3446 at 3842.330 kb on + strand, within Pf1N1B4_3446 at 3842.363 kb on - strand, within Pf1N1B4_3446 at 3842.371 kb on - strand, within Pf1N1B4_3446 at 3842.371 kb on - strand, within Pf1N1B4_3446 at 3842.467 kb on + strand at 3842.467 kb on + strand at 3842.467 kb on + strand at 3842.467 kb on + strand at 3842.467 kb on + strand at 3842.468 kb on - strand at 3842.909 kb on + strand, within Pf1N1B4_3447 at 3842.960 kb on + strand, within Pf1N1B4_3447 at 3843.046 kb on + strand, within Pf1N1B4_3447 at 3843.071 kb on + strand, within Pf1N1B4_3447 at 3843.071 kb on + strand, within Pf1N1B4_3447 at 3843.071 kb on + strand, within Pf1N1B4_3447 at 3843.071 kb on + strand, within Pf1N1B4_3447 at 3843.071 kb on + strand at 3843.071 kb on + strand at 3843.071 kb on + strand, within Pf1N1B4_3447 at 3843.072 kb on - strand, within Pf1N1B4_3447 at 3843.072 kb on - strand at 3843.072 kb on - strand, within Pf1N1B4_3447 at 3843.072 kb on - strand, within Pf1N1B4_3447 at 3843.073 kb on + strand, within Pf1N1B4_3447 at 3843.073 kb on + strand, within Pf1N1B4_3447 at 3843.074 kb on - strand, within Pf1N1B4_3447 at 3843.074 kb on - strand, within Pf1N1B4_3447 at 3843.077 kb on + strand, within Pf1N1B4_3447 at 3843.077 kb on + strand, within Pf1N1B4_3447 at 3843.077 kb on + strand, within Pf1N1B4_3447 at 3843.077 kb on + strand at 3843.078 kb on - strand, within Pf1N1B4_3447 at 3843.078 kb on - strand, within Pf1N1B4_3447 at 3843.078 kb on - strand, within Pf1N1B4_3447 at 3843.078 kb on - strand at 3843.078 kb on - strand, within Pf1N1B4_3447
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 3,840,539 + -0.0 3,840,539 + -2.3 3,840,540 - -0.0 3,840,542 + -0.0 3,840,542 + -2.2 3,840,554 + -0.0 3,840,680 - -0.0 3,840,748 + -1.2 3,840,756 - -0.0 3,840,759 + -0.0 3,840,759 + -0.1 3,840,851 - -0.2 3,841,244 + Pf1N1B4_3445 0.33 -0.0 3,841,354 + Pf1N1B4_3445 0.41 -4.1 3,841,504 + Pf1N1B4_3445 0.52 -3.5 3,841,505 - Pf1N1B4_3445 0.52 -0.0 3,841,589 - -0.8 3,841,589 - Pf1N1B4_3445 0.59 -2.7 3,841,723 + Pf1N1B4_3445 0.69 -0.0 3,842,173 + -0.0 3,842,304 + Pf1N1B4_3446 0.48 -0.5 3,842,305 - Pf1N1B4_3446 0.48 -0.0 3,842,330 + Pf1N1B4_3446 0.57 -1.2 3,842,363 - Pf1N1B4_3446 0.68 -0.0 3,842,371 - Pf1N1B4_3446 0.71 -0.0 3,842,371 - Pf1N1B4_3446 0.71 -0.0 3,842,467 + -0.0 3,842,467 + -0.0 3,842,467 + -0.0 3,842,467 + +0.3 3,842,467 + -1.5 3,842,468 - -0.0 3,842,909 + Pf1N1B4_3447 0.44 -0.0 3,842,960 + Pf1N1B4_3447 0.49 -0.8 3,843,046 + Pf1N1B4_3447 0.57 -1.0 3,843,071 + Pf1N1B4_3447 0.60 -0.0 3,843,071 + Pf1N1B4_3447 0.60 -0.0 3,843,071 + Pf1N1B4_3447 0.60 -0.0 3,843,071 + Pf1N1B4_3447 0.60 -0.0 3,843,071 + -1.0 3,843,071 + -0.0 3,843,071 + Pf1N1B4_3447 0.60 -2.9 3,843,072 - Pf1N1B4_3447 0.60 -0.0 3,843,072 - -1.0 3,843,072 - Pf1N1B4_3447 0.60 -3.2 3,843,072 - Pf1N1B4_3447 0.60 -0.0 3,843,073 + Pf1N1B4_3447 0.60 -0.0 3,843,073 + Pf1N1B4_3447 0.60 -0.0 3,843,074 - Pf1N1B4_3447 0.60 -0.0 3,843,074 - Pf1N1B4_3447 0.60 -0.0 3,843,077 + Pf1N1B4_3447 0.60 -0.0 3,843,077 + Pf1N1B4_3447 0.60 -1.5 3,843,077 + Pf1N1B4_3447 0.60 -1.1 3,843,077 + -0.0 3,843,078 - Pf1N1B4_3447 0.60 -0.0 3,843,078 - Pf1N1B4_3447 0.60 -0.0 3,843,078 - Pf1N1B4_3447 0.60 -2.6 3,843,078 - -0.0 3,843,078 - Pf1N1B4_3447 0.60 -0.0
Or see this region's nucleotide sequence