Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3403 and Pf1N1B4_3404 are separated by 111 nucleotides Pf1N1B4_3404 and Pf1N1B4_3405 overlap by 4 nucleotides Pf1N1B4_3405 and Pf1N1B4_3406 are separated by 158 nucleotides Pf1N1B4_3406 and Pf1N1B4_3407 are separated by 158 nucleotides
Pf1N1B4_3403 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 3,795,293 to 3,796,918
_3403
Pf1N1B4_3404 - FIG00953132: hypothetical protein, at 3,797,030 to 3,797,464
_3404
Pf1N1B4_3405 - Signal transduction histidine kinase, at 3,797,461 to 3,798,639
_3405
Pf1N1B4_3406 - FIG00955597: hypothetical protein, at 3,798,798 to 3,799,103
_3406
Pf1N1B4_3407 - Sterol desaturase, putative, at 3,799,262 to 3,800,149
_3407
Position (kb)
3797
3798
3799 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3796.550 kb on - strand, within Pf1N1B4_3403 at 3797.431 kb on + strand at 3797.431 kb on + strand at 3797.432 kb on - strand at 3797.432 kb on - strand at 3797.432 kb on - strand at 3797.463 kb on - strand at 3797.463 kb on - strand at 3797.564 kb on + strand at 3797.564 kb on + strand at 3797.564 kb on + strand at 3797.565 kb on - strand at 3797.565 kb on - strand at 3797.565 kb on - strand at 3797.565 kb on - strand at 3797.565 kb on - strand at 3797.764 kb on + strand, within Pf1N1B4_3405 at 3798.326 kb on + strand, within Pf1N1B4_3405 at 3798.327 kb on - strand, within Pf1N1B4_3405 at 3798.327 kb on - strand, within Pf1N1B4_3405 at 3798.341 kb on + strand at 3798.342 kb on - strand, within Pf1N1B4_3405 at 3798.342 kb on - strand, within Pf1N1B4_3405 at 3798.342 kb on - strand, within Pf1N1B4_3405 at 3798.342 kb on - strand, within Pf1N1B4_3405 at 3798.475 kb on + strand, within Pf1N1B4_3405 at 3798.475 kb on + strand, within Pf1N1B4_3405 at 3798.476 kb on - strand, within Pf1N1B4_3405 at 3798.687 kb on - strand at 3798.794 kb on + strand at 3798.794 kb on + strand at 3798.794 kb on + strand at 3798.795 kb on - strand at 3798.795 kb on - strand at 3798.795 kb on - strand at 3798.892 kb on + strand, within Pf1N1B4_3406 at 3799.130 kb on + strand at 3799.130 kb on + strand at 3799.130 kb on + strand at 3799.131 kb on - strand at 3799.159 kb on + strand at 3799.159 kb on + strand at 3799.160 kb on - strand at 3799.160 kb on - strand at 3799.160 kb on - strand at 3799.160 kb on - strand at 3799.160 kb on - strand at 3799.160 kb on - strand at 3799.160 kb on - strand at 3799.268 kb on + strand at 3799.268 kb on + strand at 3799.268 kb on + strand at 3799.268 kb on + strand at 3799.268 kb on + strand at 3799.269 kb on - strand at 3799.269 kb on - strand at 3799.269 kb on - strand at 3799.269 kb on - strand at 3799.269 kb on - strand at 3799.269 kb on - strand at 3799.269 kb on - strand at 3799.286 kb on + strand at 3799.286 kb on + strand at 3799.286 kb on + strand at 3799.286 kb on + strand at 3799.287 kb on - strand at 3799.287 kb on - strand at 3799.287 kb on - strand at 3799.287 kb on - strand at 3799.312 kb on + strand at 3799.312 kb on + strand at 3799.424 kb on + strand, within Pf1N1B4_3407
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 3,796,550 - Pf1N1B4_3403 0.77 -0.0 3,797,431 + -0.0 3,797,431 + -0.0 3,797,432 - -0.0 3,797,432 - -1.0 3,797,432 - -0.0 3,797,463 - -0.0 3,797,463 - -0.0 3,797,564 + -0.0 3,797,564 + -0.0 3,797,564 + -0.6 3,797,565 - -0.0 3,797,565 - -0.0 3,797,565 - -0.0 3,797,565 - -0.0 3,797,565 - -0.0 3,797,764 + Pf1N1B4_3405 0.26 -0.0 3,798,326 + Pf1N1B4_3405 0.73 -0.0 3,798,327 - Pf1N1B4_3405 0.73 -3.4 3,798,327 - Pf1N1B4_3405 0.73 -0.0 3,798,341 + -0.0 3,798,342 - Pf1N1B4_3405 0.75 -3.3 3,798,342 - Pf1N1B4_3405 0.75 -1.8 3,798,342 - Pf1N1B4_3405 0.75 -0.0 3,798,342 - Pf1N1B4_3405 0.75 -0.0 3,798,475 + Pf1N1B4_3405 0.86 -2.4 3,798,475 + Pf1N1B4_3405 0.86 -0.0 3,798,476 - Pf1N1B4_3405 0.86 -0.0 3,798,687 - -3.9 3,798,794 + -0.3 3,798,794 + +0.3 3,798,794 + -0.0 3,798,795 - -0.0 3,798,795 - -1.7 3,798,795 - -1.4 3,798,892 + Pf1N1B4_3406 0.31 -0.0 3,799,130 + -0.0 3,799,130 + -1.5 3,799,130 + -0.0 3,799,131 - -1.3 3,799,159 + -0.0 3,799,159 + -0.0 3,799,160 - -0.0 3,799,160 - -1.5 3,799,160 - -0.0 3,799,160 - -0.0 3,799,160 - -0.0 3,799,160 - -0.8 3,799,160 - -3.1 3,799,268 + -1.1 3,799,268 + -0.0 3,799,268 + -2.0 3,799,268 + -0.0 3,799,268 + -0.0 3,799,269 - -0.0 3,799,269 - -0.0 3,799,269 - -0.0 3,799,269 - -2.0 3,799,269 - -0.0 3,799,269 - -0.0 3,799,269 - -0.0 3,799,286 + -1.5 3,799,286 + -1.7 3,799,286 + -0.0 3,799,286 + -0.0 3,799,287 - -1.5 3,799,287 - +1.0 3,799,287 - -1.4 3,799,287 - -0.0 3,799,312 + -1.6 3,799,312 + -1.2 3,799,424 + Pf1N1B4_3407 0.18 -0.0
Or see this region's nucleotide sequence