Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3369 and Pf1N1B4_3370 are separated by 11 nucleotides Pf1N1B4_3370 and Pf1N1B4_3371 are separated by 244 nucleotides Pf1N1B4_3371 and Pf1N1B4_3372 overlap by 13 nucleotides Pf1N1B4_3372 and Pf1N1B4_3373 overlap by 4 nucleotides
Pf1N1B4_3369 - Thioredoxin reductase (EC 1.8.1.9), at 3,756,868 to 3,758,046
_3369
Pf1N1B4_3370 - ATPase associated with various cellular activities, AAA_5, at 3,758,058 to 3,758,903
_3370
Pf1N1B4_3371 - FIG00954979: hypothetical protein, at 3,759,148 to 3,759,336
_3371
Pf1N1B4_3372 - FIG00962464: hypothetical protein, at 3,759,324 to 3,759,827
_3372
Pf1N1B4_3373 - FIG00954689: hypothetical protein, at 3,759,824 to 3,760,900
_3373
Position (kb)
3758
3759 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3757.100 kb on + strand, within Pf1N1B4_3369 at 3757.139 kb on + strand, within Pf1N1B4_3369 at 3757.151 kb on + strand, within Pf1N1B4_3369 at 3757.152 kb on - strand, within Pf1N1B4_3369 at 3757.223 kb on + strand, within Pf1N1B4_3369 at 3757.223 kb on + strand, within Pf1N1B4_3369 at 3757.224 kb on - strand, within Pf1N1B4_3369 at 3757.224 kb on - strand, within Pf1N1B4_3369 at 3757.224 kb on - strand, within Pf1N1B4_3369 at 3757.224 kb on - strand, within Pf1N1B4_3369 at 3757.224 kb on - strand at 3757.241 kb on + strand, within Pf1N1B4_3369 at 3757.241 kb on + strand at 3757.241 kb on + strand, within Pf1N1B4_3369 at 3757.242 kb on - strand, within Pf1N1B4_3369 at 3757.242 kb on - strand, within Pf1N1B4_3369 at 3757.242 kb on - strand, within Pf1N1B4_3369 at 3757.242 kb on - strand, within Pf1N1B4_3369 at 3757.247 kb on + strand, within Pf1N1B4_3369 at 3757.247 kb on + strand, within Pf1N1B4_3369 at 3757.247 kb on + strand, within Pf1N1B4_3369 at 3757.248 kb on - strand at 3757.248 kb on - strand, within Pf1N1B4_3369 at 3757.467 kb on + strand, within Pf1N1B4_3369 at 3757.468 kb on - strand, within Pf1N1B4_3369 at 3757.535 kb on + strand, within Pf1N1B4_3369 at 3757.536 kb on - strand, within Pf1N1B4_3369 at 3757.854 kb on - strand, within Pf1N1B4_3369 at 3757.922 kb on + strand, within Pf1N1B4_3369 at 3757.922 kb on + strand, within Pf1N1B4_3369 at 3757.922 kb on + strand, within Pf1N1B4_3369 at 3757.922 kb on + strand, within Pf1N1B4_3369 at 3757.922 kb on + strand, within Pf1N1B4_3369 at 3757.923 kb on - strand, within Pf1N1B4_3369 at 3757.923 kb on - strand, within Pf1N1B4_3369 at 3757.923 kb on - strand, within Pf1N1B4_3369 at 3757.923 kb on - strand, within Pf1N1B4_3369 at 3757.923 kb on - strand, within Pf1N1B4_3369 at 3757.923 kb on - strand, within Pf1N1B4_3369 at 3757.923 kb on - strand, within Pf1N1B4_3369 at 3757.925 kb on + strand, within Pf1N1B4_3369 at 3757.925 kb on + strand, within Pf1N1B4_3369 at 3757.925 kb on + strand, within Pf1N1B4_3369 at 3757.925 kb on + strand, within Pf1N1B4_3369 at 3757.926 kb on - strand, within Pf1N1B4_3369 at 3757.926 kb on - strand, within Pf1N1B4_3369 at 3757.926 kb on - strand, within Pf1N1B4_3369 at 3758.271 kb on + strand, within Pf1N1B4_3370 at 3758.377 kb on + strand, within Pf1N1B4_3370 at 3758.377 kb on + strand, within Pf1N1B4_3370 at 3758.377 kb on + strand at 3758.377 kb on + strand, within Pf1N1B4_3370 at 3758.377 kb on + strand, within Pf1N1B4_3370 at 3758.378 kb on - strand, within Pf1N1B4_3370 at 3758.378 kb on - strand, within Pf1N1B4_3370 at 3758.435 kb on + strand, within Pf1N1B4_3370 at 3758.435 kb on + strand, within Pf1N1B4_3370 at 3758.436 kb on - strand, within Pf1N1B4_3370 at 3758.436 kb on - strand, within Pf1N1B4_3370 at 3758.491 kb on + strand, within Pf1N1B4_3370 at 3758.491 kb on + strand, within Pf1N1B4_3370 at 3758.491 kb on + strand, within Pf1N1B4_3370 at 3758.492 kb on - strand, within Pf1N1B4_3370 at 3758.492 kb on - strand, within Pf1N1B4_3370 at 3758.492 kb on - strand, within Pf1N1B4_3370 at 3758.623 kb on + strand, within Pf1N1B4_3370 at 3758.738 kb on + strand, within Pf1N1B4_3370 at 3758.878 kb on + strand at 3758.879 kb on - strand at 3758.879 kb on - strand at 3758.957 kb on + strand at 3758.957 kb on + strand at 3758.958 kb on - strand at 3758.958 kb on - strand at 3758.958 kb on - strand at 3758.963 kb on + strand at 3758.963 kb on + strand at 3758.964 kb on - strand at 3758.964 kb on - strand at 3758.964 kb on - strand at 3758.967 kb on - strand at 3758.974 kb on + strand at 3758.974 kb on + strand at 3758.975 kb on - strand at 3758.975 kb on - strand at 3758.975 kb on - strand at 3758.975 kb on - strand at 3759.013 kb on + strand at 3759.014 kb on - strand at 3759.014 kb on - strand at 3759.068 kb on + strand at 3759.111 kb on + strand at 3759.111 kb on + strand at 3759.111 kb on + strand at 3759.111 kb on + strand at 3759.112 kb on - strand at 3759.112 kb on - strand at 3759.112 kb on - strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.119 kb on - strand at 3759.133 kb on - strand at 3759.328 kb on + strand at 3759.561 kb on + strand, within Pf1N1B4_3372 at 3759.561 kb on + strand, within Pf1N1B4_3372 at 3759.562 kb on - strand, within Pf1N1B4_3372 at 3759.589 kb on + strand, within Pf1N1B4_3372 at 3759.606 kb on - strand, within Pf1N1B4_3372 at 3759.683 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 3,757,100 + Pf1N1B4_3369 0.20 -3.3 3,757,139 + Pf1N1B4_3369 0.23 -0.0 3,757,151 + Pf1N1B4_3369 0.24 -0.0 3,757,152 - Pf1N1B4_3369 0.24 -0.0 3,757,223 + Pf1N1B4_3369 0.30 -0.7 3,757,223 + Pf1N1B4_3369 0.30 -2.3 3,757,224 - Pf1N1B4_3369 0.30 -1.1 3,757,224 - Pf1N1B4_3369 0.30 -0.0 3,757,224 - Pf1N1B4_3369 0.30 -0.0 3,757,224 - Pf1N1B4_3369 0.30 -1.1 3,757,224 - -0.0 3,757,241 + Pf1N1B4_3369 0.32 -1.9 3,757,241 + -0.0 3,757,241 + Pf1N1B4_3369 0.32 -3.1 3,757,242 - Pf1N1B4_3369 0.32 -0.0 3,757,242 - Pf1N1B4_3369 0.32 -0.0 3,757,242 - Pf1N1B4_3369 0.32 -0.0 3,757,242 - Pf1N1B4_3369 0.32 -0.0 3,757,247 + Pf1N1B4_3369 0.32 -1.4 3,757,247 + Pf1N1B4_3369 0.32 -0.0 3,757,247 + Pf1N1B4_3369 0.32 -0.2 3,757,248 - -0.0 3,757,248 - Pf1N1B4_3369 0.32 -1.8 3,757,467 + Pf1N1B4_3369 0.51 -2.1 3,757,468 - Pf1N1B4_3369 0.51 +1.0 3,757,535 + Pf1N1B4_3369 0.57 -0.0 3,757,536 - Pf1N1B4_3369 0.57 -0.0 3,757,854 - Pf1N1B4_3369 0.84 -0.0 3,757,922 + Pf1N1B4_3369 0.89 -2.1 3,757,922 + Pf1N1B4_3369 0.89 -0.0 3,757,922 + Pf1N1B4_3369 0.89 -0.0 3,757,922 + Pf1N1B4_3369 0.89 -0.0 3,757,922 + Pf1N1B4_3369 0.89 -1.8 3,757,923 - Pf1N1B4_3369 0.89 -0.9 3,757,923 - Pf1N1B4_3369 0.89 -0.0 3,757,923 - Pf1N1B4_3369 0.89 -1.8 3,757,923 - Pf1N1B4_3369 0.89 -1.9 3,757,923 - Pf1N1B4_3369 0.89 -0.0 3,757,923 - Pf1N1B4_3369 0.89 -0.0 3,757,923 - Pf1N1B4_3369 0.89 -0.0 3,757,925 + Pf1N1B4_3369 0.90 +0.6 3,757,925 + Pf1N1B4_3369 0.90 -0.0 3,757,925 + Pf1N1B4_3369 0.90 -0.0 3,757,925 + Pf1N1B4_3369 0.90 -1.3 3,757,926 - Pf1N1B4_3369 0.90 -0.0 3,757,926 - Pf1N1B4_3369 0.90 -0.9 3,757,926 - Pf1N1B4_3369 0.90 -0.0 3,758,271 + Pf1N1B4_3370 0.25 -0.0 3,758,377 + Pf1N1B4_3370 0.38 -0.0 3,758,377 + Pf1N1B4_3370 0.38 -0.0 3,758,377 + -1.1 3,758,377 + Pf1N1B4_3370 0.38 -0.0 3,758,377 + Pf1N1B4_3370 0.38 -0.0 3,758,378 - Pf1N1B4_3370 0.38 -3.9 3,758,378 - Pf1N1B4_3370 0.38 -0.0 3,758,435 + Pf1N1B4_3370 0.45 -0.0 3,758,435 + Pf1N1B4_3370 0.45 -1.0 3,758,436 - Pf1N1B4_3370 0.45 -0.0 3,758,436 - Pf1N1B4_3370 0.45 -0.0 3,758,491 + Pf1N1B4_3370 0.51 -0.0 3,758,491 + Pf1N1B4_3370 0.51 -0.0 3,758,491 + Pf1N1B4_3370 0.51 -0.0 3,758,492 - Pf1N1B4_3370 0.51 -0.0 3,758,492 - Pf1N1B4_3370 0.51 -4.2 3,758,492 - Pf1N1B4_3370 0.51 -1.4 3,758,623 + Pf1N1B4_3370 0.67 -0.0 3,758,738 + Pf1N1B4_3370 0.80 -0.0 3,758,878 + -2.1 3,758,879 - -0.0 3,758,879 - -2.5 3,758,957 + -0.0 3,758,957 + -1.2 3,758,958 - -0.0 3,758,958 - -0.0 3,758,958 - -0.0 3,758,963 + -0.0 3,758,963 + -0.0 3,758,964 - -0.0 3,758,964 - -2.5 3,758,964 - -1.1 3,758,967 - -1.4 3,758,974 + -0.0 3,758,974 + -2.3 3,758,975 - -0.0 3,758,975 - -2.3 3,758,975 - -1.2 3,758,975 - -1.2 3,759,013 + -1.6 3,759,014 - -4.1 3,759,014 - -0.0 3,759,068 + -0.0 3,759,111 + -1.0 3,759,111 + -3.6 3,759,111 + -1.2 3,759,111 + -0.0 3,759,112 - -0.0 3,759,112 - -0.0 3,759,112 - -2.5 3,759,114 + -1.8 3,759,114 + -0.0 3,759,114 + -0.0 3,759,114 + -1.9 3,759,114 + -2.4 3,759,114 + -0.0 3,759,114 + -0.0 3,759,114 + -1.1 3,759,114 + -0.0 3,759,114 + -0.0 3,759,114 + -0.0 3,759,115 - -1.3 3,759,115 - -0.0 3,759,115 - -0.7 3,759,115 - -0.0 3,759,115 - -0.0 3,759,115 - -0.0 3,759,115 - -1.4 3,759,115 - -0.0 3,759,115 - -2.7 3,759,115 - -0.8 3,759,115 - -1.4 3,759,119 - -1.1 3,759,133 - -2.4 3,759,328 + -2.0 3,759,561 + Pf1N1B4_3372 0.47 -0.0 3,759,561 + Pf1N1B4_3372 0.47 -0.0 3,759,562 - Pf1N1B4_3372 0.47 -1.4 3,759,589 + Pf1N1B4_3372 0.53 -1.6 3,759,606 - Pf1N1B4_3372 0.56 -0.0 3,759,683 + -0.0
Or see this region's nucleotide sequence