Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3195 and Pf1N1B4_3196 are separated by 227 nucleotides Pf1N1B4_3196 and Pf1N1B4_3197 are separated by 112 nucleotides
Pf1N1B4_3195 - hydrolase, putative, at 3,576,700 to 3,579,222
_3195
Pf1N1B4_3196 - Esterase A, at 3,579,450 to 3,580,595
_3196
Pf1N1B4_3197 - OmpA family protein, at 3,580,708 to 3,581,805
_3197
Position (kb)
3579
3580
3581 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3578.561 kb on - strand, within Pf1N1B4_3195 at 3578.587 kb on + strand, within Pf1N1B4_3195 at 3578.587 kb on + strand, within Pf1N1B4_3195 at 3578.588 kb on - strand, within Pf1N1B4_3195 at 3578.718 kb on + strand, within Pf1N1B4_3195 at 3578.718 kb on + strand, within Pf1N1B4_3195 at 3578.718 kb on + strand, within Pf1N1B4_3195 at 3578.719 kb on - strand, within Pf1N1B4_3195 at 3578.719 kb on - strand, within Pf1N1B4_3195 at 3578.719 kb on - strand, within Pf1N1B4_3195 at 3578.722 kb on + strand, within Pf1N1B4_3195 at 3578.722 kb on + strand, within Pf1N1B4_3195 at 3578.722 kb on + strand, within Pf1N1B4_3195 at 3578.722 kb on + strand, within Pf1N1B4_3195 at 3578.722 kb on + strand, within Pf1N1B4_3195 at 3578.723 kb on - strand, within Pf1N1B4_3195 at 3578.723 kb on - strand, within Pf1N1B4_3195 at 3578.723 kb on - strand, within Pf1N1B4_3195 at 3578.941 kb on + strand, within Pf1N1B4_3195 at 3579.220 kb on + strand at 3579.221 kb on - strand at 3579.290 kb on + strand at 3579.348 kb on + strand at 3579.349 kb on - strand at 3579.421 kb on + strand at 3579.421 kb on + strand at 3579.421 kb on + strand at 3579.422 kb on - strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.423 kb on + strand at 3579.424 kb on - strand at 3579.424 kb on - strand at 3579.424 kb on - strand at 3579.468 kb on + strand at 3579.468 kb on + strand at 3579.469 kb on - strand at 3579.469 kb on - strand at 3579.476 kb on + strand at 3579.476 kb on + strand at 3579.476 kb on + strand at 3579.477 kb on - strand at 3579.477 kb on - strand at 3579.477 kb on - strand at 3579.477 kb on - strand at 3579.477 kb on - strand at 3579.477 kb on - strand at 3579.844 kb on - strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.930 kb on + strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.931 kb on - strand, within Pf1N1B4_3196 at 3579.952 kb on + strand, within Pf1N1B4_3196 at 3579.953 kb on - strand, within Pf1N1B4_3196 at 3579.953 kb on - strand, within Pf1N1B4_3196 at 3580.181 kb on + strand, within Pf1N1B4_3196 at 3580.181 kb on + strand, within Pf1N1B4_3196 at 3580.181 kb on + strand, within Pf1N1B4_3196 at 3580.182 kb on - strand, within Pf1N1B4_3196 at 3580.182 kb on - strand, within Pf1N1B4_3196 at 3580.182 kb on - strand, within Pf1N1B4_3196 at 3580.182 kb on - strand, within Pf1N1B4_3196 at 3580.183 kb on + strand, within Pf1N1B4_3196 at 3580.183 kb on + strand, within Pf1N1B4_3196 at 3580.190 kb on + strand, within Pf1N1B4_3196 at 3580.190 kb on + strand, within Pf1N1B4_3196 at 3580.191 kb on - strand, within Pf1N1B4_3196 at 3580.273 kb on - strand, within Pf1N1B4_3196 at 3580.273 kb on - strand, within Pf1N1B4_3196 at 3580.273 kb on - strand, within Pf1N1B4_3196 at 3580.273 kb on - strand at 3580.361 kb on - strand, within Pf1N1B4_3196 at 3580.412 kb on - strand, within Pf1N1B4_3196 at 3580.412 kb on - strand, within Pf1N1B4_3196 at 3580.495 kb on - strand at 3580.523 kb on + strand at 3580.523 kb on + strand at 3580.524 kb on - strand at 3580.524 kb on - strand at 3580.537 kb on - strand at 3580.537 kb on - strand at 3580.537 kb on - strand at 3580.537 kb on - strand at 3580.593 kb on + strand at 3580.593 kb on + strand at 3580.594 kb on - strand at 3580.594 kb on - strand at 3580.687 kb on - strand at 3580.719 kb on + strand at 3580.719 kb on + strand at 3580.974 kb on + strand, within Pf1N1B4_3197 at 3580.974 kb on + strand, within Pf1N1B4_3197 at 3581.123 kb on - strand, within Pf1N1B4_3197 at 3581.319 kb on - strand, within Pf1N1B4_3197 at 3581.319 kb on - strand, within Pf1N1B4_3197 at 3581.334 kb on + strand, within Pf1N1B4_3197 at 3581.334 kb on + strand, within Pf1N1B4_3197 at 3581.334 kb on + strand, within Pf1N1B4_3197 at 3581.334 kb on + strand, within Pf1N1B4_3197 at 3581.335 kb on - strand, within Pf1N1B4_3197 at 3581.335 kb on - strand, within Pf1N1B4_3197 at 3581.335 kb on - strand, within Pf1N1B4_3197 at 3581.335 kb on - strand, within Pf1N1B4_3197 at 3581.335 kb on - strand at 3581.335 kb on - strand, within Pf1N1B4_3197 at 3581.335 kb on - strand, within Pf1N1B4_3197 at 3581.560 kb on + strand, within Pf1N1B4_3197 at 3581.560 kb on + strand, within Pf1N1B4_3197 at 3581.560 kb on + strand, within Pf1N1B4_3197 at 3581.560 kb on + strand, within Pf1N1B4_3197 at 3581.560 kb on + strand, within Pf1N1B4_3197 at 3581.560 kb on + strand at 3581.561 kb on - strand, within Pf1N1B4_3197 at 3581.561 kb on - strand, within Pf1N1B4_3197 at 3581.561 kb on - strand, within Pf1N1B4_3197 at 3581.561 kb on - strand, within Pf1N1B4_3197
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 3,578,561 - Pf1N1B4_3195 0.74 -0.0 3,578,587 + Pf1N1B4_3195 0.75 -2.1 3,578,587 + Pf1N1B4_3195 0.75 -0.0 3,578,588 - Pf1N1B4_3195 0.75 -0.0 3,578,718 + Pf1N1B4_3195 0.80 -0.0 3,578,718 + Pf1N1B4_3195 0.80 -4.4 3,578,718 + Pf1N1B4_3195 0.80 -0.0 3,578,719 - Pf1N1B4_3195 0.80 -0.0 3,578,719 - Pf1N1B4_3195 0.80 -1.3 3,578,719 - Pf1N1B4_3195 0.80 -0.0 3,578,722 + Pf1N1B4_3195 0.80 -0.0 3,578,722 + Pf1N1B4_3195 0.80 -0.0 3,578,722 + Pf1N1B4_3195 0.80 -0.0 3,578,722 + Pf1N1B4_3195 0.80 -0.0 3,578,722 + Pf1N1B4_3195 0.80 -0.0 3,578,723 - Pf1N1B4_3195 0.80 -1.0 3,578,723 - Pf1N1B4_3195 0.80 -0.0 3,578,723 - Pf1N1B4_3195 0.80 -0.0 3,578,941 + Pf1N1B4_3195 0.89 -0.0 3,579,220 + -0.0 3,579,221 - -2.3 3,579,290 + -0.0 3,579,348 + -0.0 3,579,349 - -0.7 3,579,421 + -0.0 3,579,421 + -0.0 3,579,421 + -0.0 3,579,422 - -0.9 3,579,423 + -1.8 3,579,423 + -2.5 3,579,423 + -1.0 3,579,423 + -0.0 3,579,423 + -0.0 3,579,423 + -3.2 3,579,423 + -0.0 3,579,423 + -1.8 3,579,424 - -0.0 3,579,424 - -1.3 3,579,424 - -0.0 3,579,468 + -0.0 3,579,468 + -1.3 3,579,469 - -0.0 3,579,469 - -0.0 3,579,476 + -0.0 3,579,476 + -2.0 3,579,476 + -2.0 3,579,477 - -0.0 3,579,477 - -1.1 3,579,477 - -0.0 3,579,477 - -1.6 3,579,477 - -0.0 3,579,477 - -1.0 3,579,844 - Pf1N1B4_3196 0.34 -0.0 3,579,930 + Pf1N1B4_3196 0.42 -0.0 3,579,930 + Pf1N1B4_3196 0.42 -0.0 3,579,930 + Pf1N1B4_3196 0.42 -0.0 3,579,930 + Pf1N1B4_3196 0.42 -0.0 3,579,930 + Pf1N1B4_3196 0.42 -2.5 3,579,930 + Pf1N1B4_3196 0.42 -0.0 3,579,931 - Pf1N1B4_3196 0.42 -0.0 3,579,931 - Pf1N1B4_3196 0.42 -0.0 3,579,931 - Pf1N1B4_3196 0.42 -0.0 3,579,931 - Pf1N1B4_3196 0.42 -0.0 3,579,931 - Pf1N1B4_3196 0.42 -2.3 3,579,931 - Pf1N1B4_3196 0.42 -2.5 3,579,952 + Pf1N1B4_3196 0.44 -0.0 3,579,953 - Pf1N1B4_3196 0.44 -0.0 3,579,953 - Pf1N1B4_3196 0.44 -0.0 3,580,181 + Pf1N1B4_3196 0.64 -0.0 3,580,181 + Pf1N1B4_3196 0.64 -0.0 3,580,181 + Pf1N1B4_3196 0.64 -0.0 3,580,182 - Pf1N1B4_3196 0.64 -0.0 3,580,182 - Pf1N1B4_3196 0.64 -0.9 3,580,182 - Pf1N1B4_3196 0.64 -0.0 3,580,182 - Pf1N1B4_3196 0.64 -0.0 3,580,183 + Pf1N1B4_3196 0.64 -0.0 3,580,183 + Pf1N1B4_3196 0.64 -2.4 3,580,190 + Pf1N1B4_3196 0.65 -0.0 3,580,190 + Pf1N1B4_3196 0.65 -3.3 3,580,191 - Pf1N1B4_3196 0.65 -4.2 3,580,273 - Pf1N1B4_3196 0.72 -1.5 3,580,273 - Pf1N1B4_3196 0.72 -0.0 3,580,273 - Pf1N1B4_3196 0.72 -0.0 3,580,273 - -1.7 3,580,361 - Pf1N1B4_3196 0.79 -0.0 3,580,412 - Pf1N1B4_3196 0.84 -3.8 3,580,412 - Pf1N1B4_3196 0.84 -2.3 3,580,495 - -1.6 3,580,523 + -1.6 3,580,523 + -0.0 3,580,524 - -1.3 3,580,524 - -2.3 3,580,537 - -0.0 3,580,537 - -0.0 3,580,537 - -0.0 3,580,537 - -0.0 3,580,593 + -0.0 3,580,593 + -0.0 3,580,594 - -3.4 3,580,594 - -2.0 3,580,687 - -0.0 3,580,719 + -0.0 3,580,719 + -1.9 3,580,974 + Pf1N1B4_3197 0.24 -0.0 3,580,974 + Pf1N1B4_3197 0.24 -0.0 3,581,123 - Pf1N1B4_3197 0.38 -0.0 3,581,319 - Pf1N1B4_3197 0.56 +1.0 3,581,319 - Pf1N1B4_3197 0.56 -1.0 3,581,334 + Pf1N1B4_3197 0.57 -0.0 3,581,334 + Pf1N1B4_3197 0.57 -0.0 3,581,334 + Pf1N1B4_3197 0.57 -2.6 3,581,334 + Pf1N1B4_3197 0.57 -2.0 3,581,335 - Pf1N1B4_3197 0.57 -0.0 3,581,335 - Pf1N1B4_3197 0.57 -0.0 3,581,335 - Pf1N1B4_3197 0.57 -2.8 3,581,335 - Pf1N1B4_3197 0.57 -0.0 3,581,335 - -0.0 3,581,335 - Pf1N1B4_3197 0.57 -0.0 3,581,335 - Pf1N1B4_3197 0.57 +0.6 3,581,560 + Pf1N1B4_3197 0.78 -2.1 3,581,560 + Pf1N1B4_3197 0.78 -0.0 3,581,560 + Pf1N1B4_3197 0.78 -0.0 3,581,560 + Pf1N1B4_3197 0.78 -1.0 3,581,560 + Pf1N1B4_3197 0.78 -0.0 3,581,560 + -0.0 3,581,561 - Pf1N1B4_3197 0.78 -0.0 3,581,561 - Pf1N1B4_3197 0.78 -0.5 3,581,561 - Pf1N1B4_3197 0.78 -0.0 3,581,561 - Pf1N1B4_3197 0.78 -0.0
Or see this region's nucleotide sequence