Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2729 and Pf1N1B4_2730 are separated by 88 nucleotides Pf1N1B4_2730 and Pf1N1B4_2731 are separated by 178 nucleotides Pf1N1B4_2731 and Pf1N1B4_2732 are separated by 142 nucleotides Pf1N1B4_2732 and Pf1N1B4_2733 are separated by 22 nucleotides
Pf1N1B4_2729 - endonuclease/exonuclease/phosphatase family protein, at 3,068,505 to 3,069,587
_2729
Pf1N1B4_2730 - FIG00958173: hypothetical protein, at 3,069,676 to 3,070,350
_2730
Pf1N1B4_2731 - Uncharacterized protein conserved in bacteria, at 3,070,529 to 3,071,281
_2731
Pf1N1B4_2732 - FIG00954572: hypothetical protein, at 3,071,424 to 3,071,903
_2732
Pf1N1B4_2733 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 3,071,926 to 3,072,537
_2733
Position (kb)
3070
3071
3072 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3069.597 kb on + strand at 3069.598 kb on - strand at 3069.598 kb on - strand at 3069.599 kb on + strand at 3069.599 kb on + strand at 3069.599 kb on + strand at 3069.600 kb on - strand at 3069.600 kb on - strand at 3069.600 kb on - strand at 3069.605 kb on + strand at 3069.656 kb on + strand at 3069.816 kb on - strand, within Pf1N1B4_2730 at 3069.962 kb on + strand, within Pf1N1B4_2730 at 3070.010 kb on + strand at 3070.010 kb on + strand, within Pf1N1B4_2730 at 3070.010 kb on + strand, within Pf1N1B4_2730 at 3070.010 kb on + strand, within Pf1N1B4_2730 at 3070.010 kb on + strand, within Pf1N1B4_2730 at 3070.011 kb on - strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.165 kb on + strand, within Pf1N1B4_2730 at 3070.165 kb on + strand, within Pf1N1B4_2730 at 3070.192 kb on + strand, within Pf1N1B4_2730 at 3070.192 kb on + strand, within Pf1N1B4_2730 at 3070.193 kb on - strand, within Pf1N1B4_2730 at 3070.193 kb on - strand, within Pf1N1B4_2730 at 3070.193 kb on - strand, within Pf1N1B4_2730 at 3070.193 kb on - strand, within Pf1N1B4_2730 at 3070.316 kb on + strand at 3070.316 kb on + strand at 3070.316 kb on + strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.333 kb on + strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.388 kb on + strand at 3070.418 kb on + strand at 3070.418 kb on + strand at 3070.418 kb on + strand at 3070.418 kb on + strand at 3070.419 kb on - strand at 3070.419 kb on - strand at 3070.419 kb on - strand at 3070.452 kb on - strand at 3070.503 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.518 kb on - strand at 3070.518 kb on - strand at 3070.665 kb on - strand, within Pf1N1B4_2731 at 3070.665 kb on - strand, within Pf1N1B4_2731 at 3070.973 kb on + strand, within Pf1N1B4_2731 at 3070.974 kb on - strand, within Pf1N1B4_2731 at 3070.974 kb on - strand, within Pf1N1B4_2731 at 3070.974 kb on - strand, within Pf1N1B4_2731 at 3070.974 kb on - strand, within Pf1N1B4_2731 at 3071.072 kb on - strand, within Pf1N1B4_2731 at 3071.191 kb on + strand, within Pf1N1B4_2731 at 3071.192 kb on - strand, within Pf1N1B4_2731 at 3071.192 kb on - strand, within Pf1N1B4_2731 at 3071.192 kb on - strand, within Pf1N1B4_2731 at 3071.228 kb on + strand at 3071.229 kb on - strand at 3071.229 kb on - strand at 3071.229 kb on - strand at 3071.230 kb on + strand at 3071.355 kb on + strand at 3071.355 kb on + strand at 3071.355 kb on + strand at 3071.356 kb on - strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.463 kb on + strand at 3071.463 kb on + strand at 3071.464 kb on - strand at 3071.464 kb on - strand at 3071.464 kb on - strand at 3071.464 kb on - strand at 3071.464 kb on - strand at 3071.464 kb on - strand at 3071.466 kb on + strand at 3071.466 kb on + strand at 3071.466 kb on + strand at 3071.466 kb on + strand at 3071.466 kb on + strand at 3071.466 kb on + strand at 3071.466 kb on + strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.485 kb on - strand, within Pf1N1B4_2732 at 3071.502 kb on + strand, within Pf1N1B4_2732 at 3071.502 kb on + strand, within Pf1N1B4_2732 at 3071.503 kb on - strand, within Pf1N1B4_2732 at 3071.528 kb on + strand, within Pf1N1B4_2732 at 3071.528 kb on + strand, within Pf1N1B4_2732 at 3071.529 kb on - strand, within Pf1N1B4_2732 at 3071.529 kb on - strand, within Pf1N1B4_2732 at 3071.553 kb on + strand, within Pf1N1B4_2732 at 3071.553 kb on + strand, within Pf1N1B4_2732 at 3071.553 kb on + strand at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.643 kb on + strand, within Pf1N1B4_2732 at 3071.643 kb on + strand, within Pf1N1B4_2732 at 3071.643 kb on + strand, within Pf1N1B4_2732 at 3071.643 kb on + strand, within Pf1N1B4_2732 at 3071.644 kb on - strand, within Pf1N1B4_2732 at 3071.644 kb on - strand, within Pf1N1B4_2732 at 3071.644 kb on - strand, within Pf1N1B4_2732 at 3071.644 kb on - strand, within Pf1N1B4_2732 at 3071.790 kb on - strand, within Pf1N1B4_2732 at 3071.936 kb on + strand at 3071.936 kb on + strand at 3071.936 kb on + strand at 3071.937 kb on - strand at 3072.081 kb on + strand, within Pf1N1B4_2733 at 3072.082 kb on - strand at 3072.082 kb on - strand, within Pf1N1B4_2733 at 3072.082 kb on - strand, within Pf1N1B4_2733 at 3072.223 kb on + strand, within Pf1N1B4_2733 at 3072.224 kb on - strand, within Pf1N1B4_2733
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 3,069,597 + -0.1 3,069,598 - -0.1 3,069,598 - -0.1 3,069,599 + -1.4 3,069,599 + -0.1 3,069,599 + -0.1 3,069,600 - -0.1 3,069,600 - -1.2 3,069,600 - -0.1 3,069,605 + -0.1 3,069,656 + -0.1 3,069,816 - Pf1N1B4_2730 0.21 -3.1 3,069,962 + Pf1N1B4_2730 0.42 -0.1 3,070,010 + -0.1 3,070,010 + Pf1N1B4_2730 0.49 -0.1 3,070,010 + Pf1N1B4_2730 0.49 -1.1 3,070,010 + Pf1N1B4_2730 0.49 -1.5 3,070,010 + Pf1N1B4_2730 0.49 -1.4 3,070,011 - Pf1N1B4_2730 0.50 -0.1 3,070,163 + Pf1N1B4_2730 0.72 -1.2 3,070,163 + Pf1N1B4_2730 0.72 -0.1 3,070,163 + Pf1N1B4_2730 0.72 -0.7 3,070,163 + Pf1N1B4_2730 0.72 -2.7 3,070,163 + Pf1N1B4_2730 0.72 -0.1 3,070,163 + Pf1N1B4_2730 0.72 -0.1 3,070,164 - Pf1N1B4_2730 0.72 -0.1 3,070,164 - Pf1N1B4_2730 0.72 -0.1 3,070,164 - Pf1N1B4_2730 0.72 -1.5 3,070,164 - Pf1N1B4_2730 0.72 -0.1 3,070,164 - Pf1N1B4_2730 0.72 -1.1 3,070,164 - Pf1N1B4_2730 0.72 -0.1 3,070,165 + Pf1N1B4_2730 0.72 -0.1 3,070,165 + Pf1N1B4_2730 0.72 -0.1 3,070,192 + Pf1N1B4_2730 0.76 -1.9 3,070,192 + Pf1N1B4_2730 0.76 -0.1 3,070,193 - Pf1N1B4_2730 0.77 -0.1 3,070,193 - Pf1N1B4_2730 0.77 -0.1 3,070,193 - Pf1N1B4_2730 0.77 -0.1 3,070,193 - Pf1N1B4_2730 0.77 -0.1 3,070,316 + -0.1 3,070,316 + -0.1 3,070,316 + -0.1 3,070,317 - -1.1 3,070,317 - -0.1 3,070,317 - -0.1 3,070,317 - -0.1 3,070,317 - -0.1 3,070,317 - -0.1 3,070,333 + -0.1 3,070,334 - -1.1 3,070,334 - -0.1 3,070,334 - -0.1 3,070,334 - -0.1 3,070,334 - -0.1 3,070,334 - -0.9 3,070,334 - -0.1 3,070,334 - -1.0 3,070,334 - -0.1 3,070,388 + -0.1 3,070,418 + -1.7 3,070,418 + -0.1 3,070,418 + -1.6 3,070,418 + -1.7 3,070,419 - -0.1 3,070,419 - -0.1 3,070,419 - -0.1 3,070,452 - -0.1 3,070,503 + -1.2 3,070,517 + -0.1 3,070,517 + -0.1 3,070,517 + -0.1 3,070,517 + -1.2 3,070,517 + -0.1 3,070,518 - -1.3 3,070,518 - -0.1 3,070,665 - Pf1N1B4_2731 0.18 -0.1 3,070,665 - Pf1N1B4_2731 0.18 -2.3 3,070,973 + Pf1N1B4_2731 0.59 -2.4 3,070,974 - Pf1N1B4_2731 0.59 -0.1 3,070,974 - Pf1N1B4_2731 0.59 -1.8 3,070,974 - Pf1N1B4_2731 0.59 -0.1 3,070,974 - Pf1N1B4_2731 0.59 -0.1 3,071,072 - Pf1N1B4_2731 0.72 -0.1 3,071,191 + Pf1N1B4_2731 0.88 -0.1 3,071,192 - Pf1N1B4_2731 0.88 -1.6 3,071,192 - Pf1N1B4_2731 0.88 -0.1 3,071,192 - Pf1N1B4_2731 0.88 -0.1 3,071,228 + -0.1 3,071,229 - -0.1 3,071,229 - -0.1 3,071,229 - -1.7 3,071,230 + -1.8 3,071,355 + -0.1 3,071,355 + -1.7 3,071,355 + -0.1 3,071,356 - -0.1 3,071,420 + -0.1 3,071,420 + -1.7 3,071,420 + -0.1 3,071,420 + -2.5 3,071,420 + -1.6 3,071,420 + -0.1 3,071,420 + -1.5 3,071,420 + -0.1 3,071,420 + -0.7 3,071,421 - -0.5 3,071,421 - -0.1 3,071,421 - -0.1 3,071,421 - -0.1 3,071,421 - -2.1 3,071,421 - -0.1 3,071,421 - -0.1 3,071,421 - +0.9 3,071,463 + -0.1 3,071,463 + -0.1 3,071,464 - -0.1 3,071,464 - -0.1 3,071,464 - -1.2 3,071,464 - -0.1 3,071,464 - -0.1 3,071,464 - -1.4 3,071,466 + -0.1 3,071,466 + -2.3 3,071,466 + -0.1 3,071,466 + -0.1 3,071,466 + -0.1 3,071,466 + -0.0 3,071,466 + -0.1 3,071,467 - -0.1 3,071,467 - -1.7 3,071,467 - -0.1 3,071,467 - -0.1 3,071,467 - -0.1 3,071,467 - -0.1 3,071,467 - -1.9 3,071,467 - -0.3 3,071,485 - Pf1N1B4_2732 0.13 -2.4 3,071,502 + Pf1N1B4_2732 0.16 -0.7 3,071,502 + Pf1N1B4_2732 0.16 -0.1 3,071,503 - Pf1N1B4_2732 0.16 -0.1 3,071,528 + Pf1N1B4_2732 0.22 -0.1 3,071,528 + Pf1N1B4_2732 0.22 -0.1 3,071,529 - Pf1N1B4_2732 0.22 -1.7 3,071,529 - Pf1N1B4_2732 0.22 -0.1 3,071,553 + Pf1N1B4_2732 0.27 -2.5 3,071,553 + Pf1N1B4_2732 0.27 -0.1 3,071,553 + -0.1 3,071,554 - Pf1N1B4_2732 0.27 -0.1 3,071,554 - -0.1 3,071,554 - Pf1N1B4_2732 0.27 -0.1 3,071,554 - Pf1N1B4_2732 0.27 -0.1 3,071,554 - Pf1N1B4_2732 0.27 +0.9 3,071,554 - Pf1N1B4_2732 0.27 -0.3 3,071,554 - -0.1 3,071,554 - Pf1N1B4_2732 0.27 -0.1 3,071,554 - Pf1N1B4_2732 0.27 -0.1 3,071,554 - Pf1N1B4_2732 0.27 -0.1 3,071,643 + Pf1N1B4_2732 0.46 -1.5 3,071,643 + Pf1N1B4_2732 0.46 -0.1 3,071,643 + Pf1N1B4_2732 0.46 -0.1 3,071,643 + Pf1N1B4_2732 0.46 -4.2 3,071,644 - Pf1N1B4_2732 0.46 -0.6 3,071,644 - Pf1N1B4_2732 0.46 -0.1 3,071,644 - Pf1N1B4_2732 0.46 -2.0 3,071,644 - Pf1N1B4_2732 0.46 -2.3 3,071,790 - Pf1N1B4_2732 0.76 -1.3 3,071,936 + -0.1 3,071,936 + -0.1 3,071,936 + -0.1 3,071,937 - -1.0 3,072,081 + Pf1N1B4_2733 0.25 -0.1 3,072,082 - -2.3 3,072,082 - Pf1N1B4_2733 0.25 -0.1 3,072,082 - Pf1N1B4_2733 0.25 -2.5 3,072,223 + Pf1N1B4_2733 0.49 -1.5 3,072,224 - Pf1N1B4_2733 0.49 -0.1
Or see this region's nucleotide sequence