Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2725 and Pf1N1B4_2726 are separated by 39 nucleotides Pf1N1B4_2726 and Pf1N1B4_2727 are separated by 30 nucleotides Pf1N1B4_2727 and Pf1N1B4_2728 are separated by 97 nucleotides
Pf1N1B4_2725 - FIG00954854: hypothetical protein, at 3,063,371 to 3,064,366
_2725
Pf1N1B4_2726 - Cobalt-zinc-cadmium resistance protein, at 3,064,406 to 3,065,299
_2726
Pf1N1B4_2727 - Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase), at 3,065,330 to 3,065,749
_2727
Pf1N1B4_2728 - ATP-dependent helicase HrpB, at 3,065,847 to 3,068,363
_2728
Position (kb)
3065
3066 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3064.378 kb on + strand at 3064.378 kb on + strand at 3064.378 kb on + strand at 3064.378 kb on + strand at 3064.378 kb on + strand at 3064.378 kb on + strand at 3064.378 kb on + strand at 3064.378 kb on + strand at 3064.378 kb on + strand at 3064.378 kb on + strand at 3064.378 kb on + strand at 3064.378 kb on + strand at 3064.379 kb on - strand at 3064.379 kb on - strand at 3064.379 kb on - strand at 3064.379 kb on - strand at 3064.379 kb on - strand at 3064.381 kb on - strand at 3064.381 kb on - strand at 3064.381 kb on - strand at 3064.407 kb on + strand at 3064.407 kb on + strand at 3064.435 kb on + strand at 3064.435 kb on + strand at 3064.436 kb on - strand at 3064.436 kb on - strand at 3064.436 kb on - strand at 3064.477 kb on + strand at 3064.477 kb on + strand at 3064.477 kb on + strand at 3064.478 kb on - strand at 3064.478 kb on - strand at 3064.728 kb on + strand, within Pf1N1B4_2726 at 3064.728 kb on + strand, within Pf1N1B4_2726 at 3064.728 kb on + strand, within Pf1N1B4_2726 at 3064.728 kb on + strand, within Pf1N1B4_2726 at 3064.729 kb on - strand, within Pf1N1B4_2726 at 3065.110 kb on - strand, within Pf1N1B4_2726 at 3065.110 kb on - strand, within Pf1N1B4_2726 at 3065.267 kb on + strand at 3065.299 kb on + strand at 3065.299 kb on + strand at 3065.299 kb on + strand at 3065.300 kb on - strand at 3065.300 kb on - strand at 3065.300 kb on - strand at 3065.300 kb on - strand at 3065.440 kb on + strand, within Pf1N1B4_2727 at 3065.440 kb on + strand, within Pf1N1B4_2727 at 3065.441 kb on - strand, within Pf1N1B4_2727 at 3065.441 kb on - strand, within Pf1N1B4_2727 at 3065.441 kb on - strand, within Pf1N1B4_2727 at 3065.533 kb on - strand, within Pf1N1B4_2727 at 3065.533 kb on - strand, within Pf1N1B4_2727 at 3065.533 kb on - strand, within Pf1N1B4_2727 at 3065.535 kb on + strand, within Pf1N1B4_2727 at 3065.535 kb on + strand, within Pf1N1B4_2727 at 3065.535 kb on + strand, within Pf1N1B4_2727 at 3065.536 kb on - strand at 3065.536 kb on - strand, within Pf1N1B4_2727 at 3065.588 kb on - strand, within Pf1N1B4_2727 at 3065.589 kb on + strand, within Pf1N1B4_2727 at 3065.589 kb on + strand, within Pf1N1B4_2727 at 3065.589 kb on + strand, within Pf1N1B4_2727 at 3065.590 kb on - strand, within Pf1N1B4_2727 at 3065.590 kb on - strand, within Pf1N1B4_2727 at 3065.717 kb on + strand at 3065.717 kb on + strand at 3065.717 kb on + strand at 3065.797 kb on + strand at 3065.797 kb on + strand at 3065.797 kb on + strand at 3065.797 kb on + strand at 3065.798 kb on - strand at 3065.798 kb on - strand at 3065.798 kb on - strand at 3065.819 kb on - strand at 3065.820 kb on + strand at 3065.820 kb on + strand at 3065.820 kb on + strand at 3065.821 kb on - strand at 3065.821 kb on - strand at 3065.821 kb on - strand at 3065.821 kb on - strand at 3065.821 kb on - strand at 3065.900 kb on + strand at 3066.314 kb on - strand, within Pf1N1B4_2728 at 3066.314 kb on - strand, within Pf1N1B4_2728 at 3066.314 kb on - strand, within Pf1N1B4_2728 at 3066.314 kb on - strand, within Pf1N1B4_2728 at 3066.396 kb on + strand, within Pf1N1B4_2728 at 3066.397 kb on - strand, within Pf1N1B4_2728 at 3066.443 kb on + strand, within Pf1N1B4_2728
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 3,064,378 + -3.6 3,064,378 + -0.1 3,064,378 + -0.1 3,064,378 + -1.1 3,064,378 + -0.1 3,064,378 + -1.1 3,064,378 + -0.1 3,064,378 + -0.1 3,064,378 + -0.1 3,064,378 + -0.1 3,064,378 + -0.1 3,064,378 + -0.1 3,064,379 - -0.1 3,064,379 - -1.6 3,064,379 - -0.1 3,064,379 - -0.1 3,064,379 - +0.9 3,064,381 - -0.1 3,064,381 - -0.1 3,064,381 - -0.1 3,064,407 + -0.1 3,064,407 + -1.8 3,064,435 + -1.7 3,064,435 + -0.1 3,064,436 - -2.0 3,064,436 - -0.1 3,064,436 - -0.1 3,064,477 + -0.1 3,064,477 + -0.9 3,064,477 + -1.6 3,064,478 - -0.1 3,064,478 - -1.1 3,064,728 + Pf1N1B4_2726 0.36 -0.1 3,064,728 + Pf1N1B4_2726 0.36 -0.1 3,064,728 + Pf1N1B4_2726 0.36 -1.5 3,064,728 + Pf1N1B4_2726 0.36 -0.1 3,064,729 - Pf1N1B4_2726 0.36 -0.1 3,065,110 - Pf1N1B4_2726 0.79 -0.1 3,065,110 - Pf1N1B4_2726 0.79 -0.1 3,065,267 + -0.1 3,065,299 + -0.1 3,065,299 + -0.1 3,065,299 + -0.1 3,065,300 - -0.1 3,065,300 - -0.1 3,065,300 - -1.9 3,065,300 - -2.8 3,065,440 + Pf1N1B4_2727 0.26 -0.1 3,065,440 + Pf1N1B4_2727 0.26 -0.1 3,065,441 - Pf1N1B4_2727 0.26 -0.1 3,065,441 - Pf1N1B4_2727 0.26 -2.3 3,065,441 - Pf1N1B4_2727 0.26 -0.1 3,065,533 - Pf1N1B4_2727 0.48 -0.1 3,065,533 - Pf1N1B4_2727 0.48 -3.6 3,065,533 - Pf1N1B4_2727 0.48 -0.1 3,065,535 + Pf1N1B4_2727 0.49 -0.1 3,065,535 + Pf1N1B4_2727 0.49 -0.1 3,065,535 + Pf1N1B4_2727 0.49 -0.1 3,065,536 - -0.1 3,065,536 - Pf1N1B4_2727 0.49 -0.1 3,065,588 - Pf1N1B4_2727 0.61 -0.1 3,065,589 + Pf1N1B4_2727 0.62 -0.1 3,065,589 + Pf1N1B4_2727 0.62 -0.1 3,065,589 + Pf1N1B4_2727 0.62 -0.1 3,065,590 - Pf1N1B4_2727 0.62 -0.1 3,065,590 - Pf1N1B4_2727 0.62 -0.1 3,065,717 + -0.1 3,065,717 + -0.1 3,065,717 + -0.1 3,065,797 + -1.4 3,065,797 + -0.1 3,065,797 + -1.7 3,065,797 + -1.7 3,065,798 - -0.1 3,065,798 - -3.1 3,065,798 - -0.1 3,065,819 - -0.9 3,065,820 + -0.1 3,065,820 + -0.1 3,065,820 + -0.1 3,065,821 - -0.1 3,065,821 - -0.1 3,065,821 - -1.1 3,065,821 - -0.1 3,065,821 - -0.1 3,065,900 + -0.1 3,066,314 - Pf1N1B4_2728 0.19 -0.1 3,066,314 - Pf1N1B4_2728 0.19 -0.1 3,066,314 - Pf1N1B4_2728 0.19 -0.1 3,066,314 - Pf1N1B4_2728 0.19 -0.1 3,066,396 + Pf1N1B4_2728 0.22 -0.7 3,066,397 - Pf1N1B4_2728 0.22 -0.1 3,066,443 + Pf1N1B4_2728 0.24 -0.1
Or see this region's nucleotide sequence