Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2250 and Pf1N1B4_2251 overlap by 4 nucleotides Pf1N1B4_2251 and Pf1N1B4_2252 are separated by 34 nucleotides Pf1N1B4_2252 and Pf1N1B4_2253 are separated by 152 nucleotides
Pf1N1B4_2250 - Alkanesulfonates transport system permease protein, at 2,566,731 to 2,567,513
_2250
Pf1N1B4_2251 - Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB, at 2,567,510 to 2,568,316
_2251
Pf1N1B4_2252 - Organosulfonate utilization protein SsuF, at 2,568,351 to 2,568,566
_2252
Pf1N1B4_2253 - Transcriptional regulator, TetR family, at 2,568,719 to 2,569,351
_2253
Position (kb)
2568
2569 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2567.409 kb on + strand, within Pf1N1B4_2250 at 2567.409 kb on + strand, within Pf1N1B4_2250 at 2567.409 kb on + strand, within Pf1N1B4_2250 at 2567.671 kb on + strand, within Pf1N1B4_2251 at 2567.671 kb on + strand, within Pf1N1B4_2251 at 2567.672 kb on - strand, within Pf1N1B4_2251 at 2568.293 kb on - strand at 2568.314 kb on + strand at 2568.315 kb on - strand at 2568.315 kb on - strand at 2568.315 kb on - strand at 2568.319 kb on + strand at 2568.319 kb on + strand at 2568.319 kb on + strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.356 kb on + strand at 2568.357 kb on - strand at 2568.357 kb on - strand at 2568.378 kb on - strand, within Pf1N1B4_2252 at 2568.474 kb on - strand, within Pf1N1B4_2252 at 2568.520 kb on + strand, within Pf1N1B4_2252 at 2568.520 kb on + strand, within Pf1N1B4_2252 at 2568.520 kb on + strand, within Pf1N1B4_2252 at 2568.521 kb on - strand, within Pf1N1B4_2252 at 2568.564 kb on + strand at 2568.564 kb on + strand at 2568.564 kb on + strand at 2568.564 kb on + strand at 2568.564 kb on + strand at 2568.565 kb on - strand at 2568.576 kb on + strand at 2568.741 kb on + strand at 2568.742 kb on - strand at 2568.742 kb on - strand at 2568.742 kb on - strand at 2568.754 kb on - strand at 2568.754 kb on - strand at 2568.754 kb on - strand at 2568.765 kb on + strand at 2568.765 kb on + strand at 2568.766 kb on - strand at 2568.766 kb on - strand at 2568.766 kb on - strand at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2569.035 kb on + strand, within Pf1N1B4_2253 at 2569.035 kb on + strand, within Pf1N1B4_2253 at 2569.035 kb on + strand, within Pf1N1B4_2253 at 2569.036 kb on - strand, within Pf1N1B4_2253 at 2569.233 kb on + strand, within Pf1N1B4_2253 at 2569.381 kb on + strand at 2569.381 kb on + strand at 2569.381 kb on + strand at 2569.382 kb on - strand at 2569.382 kb on - strand at 2569.382 kb on - strand at 2569.429 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.494 kb on - strand at 2569.524 kb on + strand at 2569.524 kb on + strand at 2569.545 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 2,567,409 + Pf1N1B4_2250 0.87 -1.4 2,567,409 + Pf1N1B4_2250 0.87 -2.0 2,567,409 + Pf1N1B4_2250 0.87 +1.0 2,567,671 + Pf1N1B4_2251 0.20 -0.0 2,567,671 + Pf1N1B4_2251 0.20 -2.1 2,567,672 - Pf1N1B4_2251 0.20 -0.0 2,568,293 - -0.0 2,568,314 + -1.7 2,568,315 - -2.7 2,568,315 - -2.2 2,568,315 - -0.0 2,568,319 + -1.2 2,568,319 + -2.4 2,568,319 + -0.0 2,568,320 - -0.0 2,568,320 - -0.0 2,568,320 - -0.0 2,568,320 - -0.0 2,568,320 - -0.0 2,568,356 + -1.6 2,568,357 - -0.0 2,568,357 - -2.8 2,568,378 - Pf1N1B4_2252 0.12 -0.0 2,568,474 - Pf1N1B4_2252 0.57 -2.3 2,568,520 + Pf1N1B4_2252 0.78 -2.4 2,568,520 + Pf1N1B4_2252 0.78 -0.0 2,568,520 + Pf1N1B4_2252 0.78 -0.0 2,568,521 - Pf1N1B4_2252 0.79 -0.0 2,568,564 + -1.2 2,568,564 + -0.0 2,568,564 + -0.0 2,568,564 + -1.3 2,568,564 + -0.0 2,568,565 - -1.2 2,568,576 + -0.0 2,568,741 + -4.4 2,568,742 - -0.0 2,568,742 - -0.0 2,568,742 - -0.0 2,568,754 - -0.0 2,568,754 - -2.5 2,568,754 - -0.0 2,568,765 + -1.0 2,568,765 + -0.4 2,568,766 - -1.5 2,568,766 - -0.0 2,568,766 - -2.3 2,568,840 + Pf1N1B4_2253 0.19 +1.0 2,568,840 + Pf1N1B4_2253 0.19 -3.2 2,568,840 + Pf1N1B4_2253 0.19 -0.0 2,568,840 + Pf1N1B4_2253 0.19 -1.1 2,568,840 + Pf1N1B4_2253 0.19 -1.8 2,568,840 + Pf1N1B4_2253 0.19 -0.0 2,568,840 + -1.4 2,568,840 + Pf1N1B4_2253 0.19 -0.0 2,568,840 + Pf1N1B4_2253 0.19 -0.0 2,568,840 + Pf1N1B4_2253 0.19 -0.0 2,568,840 + Pf1N1B4_2253 0.19 -0.0 2,568,840 + Pf1N1B4_2253 0.19 -0.9 2,568,840 + Pf1N1B4_2253 0.19 -0.0 2,568,840 + Pf1N1B4_2253 0.19 -0.0 2,568,841 - Pf1N1B4_2253 0.19 -0.0 2,568,841 - Pf1N1B4_2253 0.19 -2.3 2,568,841 - Pf1N1B4_2253 0.19 -3.7 2,568,841 - Pf1N1B4_2253 0.19 -0.0 2,568,841 - Pf1N1B4_2253 0.19 -2.0 2,568,841 - -3.5 2,568,841 - Pf1N1B4_2253 0.19 -0.0 2,568,841 - Pf1N1B4_2253 0.19 -0.0 2,568,841 - Pf1N1B4_2253 0.19 -0.0 2,569,035 + Pf1N1B4_2253 0.50 -0.0 2,569,035 + Pf1N1B4_2253 0.50 -0.0 2,569,035 + Pf1N1B4_2253 0.50 -0.0 2,569,036 - Pf1N1B4_2253 0.50 -1.6 2,569,233 + Pf1N1B4_2253 0.81 -0.0 2,569,381 + -0.0 2,569,381 + -0.0 2,569,381 + -0.0 2,569,382 - -2.1 2,569,382 - -2.4 2,569,382 - -0.0 2,569,429 + -0.0 2,569,464 + -0.0 2,569,464 + -0.0 2,569,464 + -0.0 2,569,464 + -0.0 2,569,464 + -0.0 2,569,464 + -0.0 2,569,464 + -0.0 2,569,464 + -0.0 2,569,464 + -0.0 2,569,464 + -2.0 2,569,464 + -0.0 2,569,464 + -2.7 2,569,464 + -1.2 2,569,465 - -0.0 2,569,465 - -0.0 2,569,465 - -1.5 2,569,465 - -0.0 2,569,465 - -0.0 2,569,465 - -0.9 2,569,465 - -1.9 2,569,465 - -0.0 2,569,465 - -0.0 2,569,465 - -0.0 2,569,465 - -0.0 2,569,465 - -0.0 2,569,465 - -2.1 2,569,465 - -1.6 2,569,465 - -0.0 2,569,494 - -0.0 2,569,524 + -0.0 2,569,524 + -0.0 2,569,545 + -0.0
Or see this region's nucleotide sequence