Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2082 and Pf1N1B4_2083 are separated by 32 nucleotides Pf1N1B4_2083 and Pf1N1B4_2084 are separated by 28 nucleotides
Pf1N1B4_2082 - Uncharacterized protein ImpA, at 2,382,475 to 2,384,031
_2082
Pf1N1B4_2083 - Uncharacterized protein ImpB, at 2,384,064 to 2,384,567
_2083
Pf1N1B4_2084 - Uncharacterized protein ImpC, at 2,384,596 to 2,386,071
_2084
Position (kb)
2384
2385 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2383.363 kb on + strand, within Pf1N1B4_2082 at 2383.364 kb on - strand, within Pf1N1B4_2082 at 2383.364 kb on - strand, within Pf1N1B4_2082 at 2383.379 kb on + strand, within Pf1N1B4_2082 at 2383.379 kb on + strand at 2383.379 kb on + strand, within Pf1N1B4_2082 at 2383.433 kb on + strand, within Pf1N1B4_2082 at 2383.434 kb on - strand, within Pf1N1B4_2082 at 2383.434 kb on - strand, within Pf1N1B4_2082 at 2383.822 kb on + strand, within Pf1N1B4_2082 at 2383.823 kb on - strand, within Pf1N1B4_2082 at 2383.866 kb on - strand, within Pf1N1B4_2082 at 2383.866 kb on - strand, within Pf1N1B4_2082 at 2383.929 kb on + strand at 2383.929 kb on + strand at 2383.929 kb on + strand at 2383.930 kb on - strand at 2383.930 kb on - strand at 2383.987 kb on + strand at 2384.021 kb on + strand at 2384.030 kb on - strand at 2384.030 kb on - strand at 2384.102 kb on + strand at 2384.164 kb on + strand, within Pf1N1B4_2083 at 2384.164 kb on + strand, within Pf1N1B4_2083 at 2384.164 kb on + strand, within Pf1N1B4_2083 at 2384.164 kb on + strand, within Pf1N1B4_2083 at 2384.165 kb on - strand, within Pf1N1B4_2083 at 2384.181 kb on + strand, within Pf1N1B4_2083 at 2384.182 kb on - strand, within Pf1N1B4_2083 at 2384.182 kb on - strand, within Pf1N1B4_2083 at 2384.182 kb on - strand, within Pf1N1B4_2083 at 2384.574 kb on - strand at 2384.625 kb on + strand at 2384.641 kb on + strand at 2384.641 kb on + strand at 2384.641 kb on + strand at 2384.699 kb on - strand at 2385.010 kb on + strand, within Pf1N1B4_2084 at 2385.010 kb on + strand, within Pf1N1B4_2084 at 2385.010 kb on + strand, within Pf1N1B4_2084 at 2385.011 kb on - strand, within Pf1N1B4_2084 at 2385.022 kb on + strand, within Pf1N1B4_2084 at 2385.022 kb on + strand, within Pf1N1B4_2084 at 2385.022 kb on + strand, within Pf1N1B4_2084 at 2385.023 kb on - strand, within Pf1N1B4_2084 at 2385.023 kb on - strand, within Pf1N1B4_2084 at 2385.023 kb on - strand, within Pf1N1B4_2084 at 2385.023 kb on - strand, within Pf1N1B4_2084 at 2385.023 kb on - strand, within Pf1N1B4_2084 at 2385.079 kb on + strand, within Pf1N1B4_2084 at 2385.079 kb on + strand, within Pf1N1B4_2084 at 2385.079 kb on + strand, within Pf1N1B4_2084 at 2385.080 kb on - strand, within Pf1N1B4_2084 at 2385.080 kb on - strand, within Pf1N1B4_2084 at 2385.082 kb on + strand, within Pf1N1B4_2084 at 2385.082 kb on + strand, within Pf1N1B4_2084 at 2385.082 kb on + strand at 2385.082 kb on + strand, within Pf1N1B4_2084 at 2385.082 kb on + strand, within Pf1N1B4_2084 at 2385.082 kb on + strand, within Pf1N1B4_2084 at 2385.082 kb on + strand, within Pf1N1B4_2084 at 2385.082 kb on + strand, within Pf1N1B4_2084 at 2385.082 kb on + strand, within Pf1N1B4_2084 at 2385.083 kb on - strand, within Pf1N1B4_2084 at 2385.083 kb on - strand, within Pf1N1B4_2084 at 2385.083 kb on - strand, within Pf1N1B4_2084 at 2385.083 kb on - strand, within Pf1N1B4_2084 at 2385.083 kb on - strand, within Pf1N1B4_2084 at 2385.083 kb on - strand, within Pf1N1B4_2084 at 2385.083 kb on - strand at 2385.121 kb on + strand, within Pf1N1B4_2084 at 2385.121 kb on + strand, within Pf1N1B4_2084 at 2385.121 kb on + strand, within Pf1N1B4_2084 at 2385.122 kb on - strand, within Pf1N1B4_2084 at 2385.122 kb on - strand, within Pf1N1B4_2084 at 2385.122 kb on - strand, within Pf1N1B4_2084 at 2385.122 kb on - strand at 2385.122 kb on - strand, within Pf1N1B4_2084 at 2385.122 kb on - strand, within Pf1N1B4_2084 at 2385.373 kb on + strand, within Pf1N1B4_2084 at 2385.374 kb on - strand, within Pf1N1B4_2084 at 2385.374 kb on - strand, within Pf1N1B4_2084 at 2385.406 kb on + strand, within Pf1N1B4_2084 at 2385.406 kb on + strand, within Pf1N1B4_2084 at 2385.406 kb on + strand, within Pf1N1B4_2084 at 2385.406 kb on + strand, within Pf1N1B4_2084 at 2385.407 kb on - strand, within Pf1N1B4_2084 at 2385.407 kb on - strand, within Pf1N1B4_2084 at 2385.407 kb on - strand, within Pf1N1B4_2084 at 2385.428 kb on - strand, within Pf1N1B4_2084
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 2,383,363 + Pf1N1B4_2082 0.57 -0.7 2,383,364 - Pf1N1B4_2082 0.57 -2.5 2,383,364 - Pf1N1B4_2082 0.57 +0.0 2,383,379 + Pf1N1B4_2082 0.58 -2.6 2,383,379 + +0.0 2,383,379 + Pf1N1B4_2082 0.58 -0.9 2,383,433 + Pf1N1B4_2082 0.62 -0.9 2,383,434 - Pf1N1B4_2082 0.62 +0.0 2,383,434 - Pf1N1B4_2082 0.62 +0.0 2,383,822 + Pf1N1B4_2082 0.87 +0.0 2,383,823 - Pf1N1B4_2082 0.87 +0.0 2,383,866 - Pf1N1B4_2082 0.89 +0.0 2,383,866 - Pf1N1B4_2082 0.89 +0.0 2,383,929 + +0.0 2,383,929 + -3.0 2,383,929 + +0.0 2,383,930 - +0.0 2,383,930 - +1.0 2,383,987 + -1.7 2,384,021 + +0.0 2,384,030 - +0.0 2,384,030 - +0.0 2,384,102 + +0.0 2,384,164 + Pf1N1B4_2083 0.20 -2.3 2,384,164 + Pf1N1B4_2083 0.20 +0.0 2,384,164 + Pf1N1B4_2083 0.20 +0.0 2,384,164 + Pf1N1B4_2083 0.20 +0.0 2,384,165 - Pf1N1B4_2083 0.20 -2.3 2,384,181 + Pf1N1B4_2083 0.23 -2.1 2,384,182 - Pf1N1B4_2083 0.23 +0.0 2,384,182 - Pf1N1B4_2083 0.23 +0.0 2,384,182 - Pf1N1B4_2083 0.23 +0.0 2,384,574 - +0.0 2,384,625 + +0.0 2,384,641 + -2.0 2,384,641 + -1.1 2,384,641 + +0.0 2,384,699 - -1.6 2,385,010 + Pf1N1B4_2084 0.28 -0.0 2,385,010 + Pf1N1B4_2084 0.28 -1.7 2,385,010 + Pf1N1B4_2084 0.28 -0.0 2,385,011 - Pf1N1B4_2084 0.28 -0.0 2,385,022 + Pf1N1B4_2084 0.29 -0.0 2,385,022 + Pf1N1B4_2084 0.29 -0.0 2,385,022 + Pf1N1B4_2084 0.29 -0.0 2,385,023 - Pf1N1B4_2084 0.29 -0.0 2,385,023 - Pf1N1B4_2084 0.29 -3.4 2,385,023 - Pf1N1B4_2084 0.29 +1.0 2,385,023 - Pf1N1B4_2084 0.29 -0.0 2,385,023 - Pf1N1B4_2084 0.29 -0.0 2,385,079 + Pf1N1B4_2084 0.33 -0.0 2,385,079 + Pf1N1B4_2084 0.33 -0.4 2,385,079 + Pf1N1B4_2084 0.33 -1.7 2,385,080 - Pf1N1B4_2084 0.33 -1.9 2,385,080 - Pf1N1B4_2084 0.33 -0.0 2,385,082 + Pf1N1B4_2084 0.33 -0.0 2,385,082 + Pf1N1B4_2084 0.33 -0.0 2,385,082 + -0.9 2,385,082 + Pf1N1B4_2084 0.33 -1.0 2,385,082 + Pf1N1B4_2084 0.33 -0.0 2,385,082 + Pf1N1B4_2084 0.33 -0.9 2,385,082 + Pf1N1B4_2084 0.33 -1.2 2,385,082 + Pf1N1B4_2084 0.33 -0.0 2,385,082 + Pf1N1B4_2084 0.33 -0.0 2,385,083 - Pf1N1B4_2084 0.33 -1.0 2,385,083 - Pf1N1B4_2084 0.33 -0.0 2,385,083 - Pf1N1B4_2084 0.33 -0.0 2,385,083 - Pf1N1B4_2084 0.33 -0.0 2,385,083 - Pf1N1B4_2084 0.33 -0.0 2,385,083 - Pf1N1B4_2084 0.33 -0.0 2,385,083 - -0.8 2,385,121 + Pf1N1B4_2084 0.36 -0.0 2,385,121 + Pf1N1B4_2084 0.36 -1.0 2,385,121 + Pf1N1B4_2084 0.36 -0.0 2,385,122 - Pf1N1B4_2084 0.36 -0.0 2,385,122 - Pf1N1B4_2084 0.36 -1.3 2,385,122 - Pf1N1B4_2084 0.36 -0.0 2,385,122 - -2.4 2,385,122 - Pf1N1B4_2084 0.36 -0.0 2,385,122 - Pf1N1B4_2084 0.36 -2.0 2,385,373 + Pf1N1B4_2084 0.53 -2.0 2,385,374 - Pf1N1B4_2084 0.53 -2.0 2,385,374 - Pf1N1B4_2084 0.53 -0.0 2,385,406 + Pf1N1B4_2084 0.55 -0.0 2,385,406 + Pf1N1B4_2084 0.55 -0.0 2,385,406 + Pf1N1B4_2084 0.55 -0.0 2,385,406 + Pf1N1B4_2084 0.55 -1.2 2,385,407 - Pf1N1B4_2084 0.55 -0.0 2,385,407 - Pf1N1B4_2084 0.55 -0.0 2,385,407 - Pf1N1B4_2084 0.55 -0.0 2,385,428 - Pf1N1B4_2084 0.56 -0.0
Or see this region's nucleotide sequence