Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_2053 and Pf1N1B4_2054 are separated by 12 nucleotides Pf1N1B4_2054 and Pf1N1B4_2055 are separated by 18 nucleotides 
        Pf1N1B4_2053 - Rhs-family protein, at 2,339,640 to 2,344,013 
        _2053 
         
        
        Pf1N1B4_2054 - FIG00964469: hypothetical protein, at 2,344,026 to 2,344,460 
        _2054 
         
        
        Pf1N1B4_2055 - VgrG-3 protein, at 2,344,479 to 2,346,416 
        _2055 
         Position (kb) 2344 
2345 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 at 2343.026 kb on - strand, within Pf1N1B4_2053 at 2343.026 kb on - strand, within Pf1N1B4_2053 at 2343.381 kb on - strand, within Pf1N1B4_2053 at 2343.381 kb on - strand, within Pf1N1B4_2053 at 2343.381 kb on - strand, within Pf1N1B4_2053 at 2343.577 kb on + strand at 2343.577 kb on + strand at 2343.577 kb on + strand at 2343.577 kb on + strand at 2343.577 kb on + strand at 2343.578 kb on - strand at 2343.578 kb on - strand at 2343.578 kb on - strand at 2343.707 kb on + strand at 2343.707 kb on + strand at 2343.707 kb on + strand at 2343.708 kb on - strand at 2343.708 kb on - strand at 2343.708 kb on - strand at 2344.111 kb on + strand, within Pf1N1B4_2054 at 2344.111 kb on + strand, within Pf1N1B4_2054 at 2344.112 kb on - strand, within Pf1N1B4_2054 at 2344.112 kb on - strand, within Pf1N1B4_2054 at 2344.112 kb on - strand, within Pf1N1B4_2054 at 2344.453 kb on + strand at 2344.453 kb on + strand at 2344.454 kb on - strand at 2344.624 kb on + strand at 2344.624 kb on + strand at 2344.624 kb on + strand at 2344.625 kb on - strand at 2344.625 kb on - strand at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.730 kb on - strand, within Pf1N1B4_2055 at 2344.730 kb on - strand, within Pf1N1B4_2055 at 2344.730 kb on - strand, within Pf1N1B4_2055 at 2344.730 kb on - strand, within Pf1N1B4_2055 at 2344.730 kb on - strand at 2344.730 kb on - strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.919 kb on - strand, within Pf1N1B4_2055 at 2344.919 kb on - strand, within Pf1N1B4_2055 at 2345.060 kb on - strand, within Pf1N1B4_2055 at 2345.230 kb on + strand, within Pf1N1B4_2055 at 2345.230 kb on + strand, within Pf1N1B4_2055 at 2345.230 kb on + strand, within Pf1N1B4_2055 at 2345.321 kb on + strand, within Pf1N1B4_2055 at 2345.351 kb on - strand, within Pf1N1B4_2055 at 2345.440 kb on + strand, within Pf1N1B4_2055 at 2345.440 kb on + strand, within Pf1N1B4_2055 at 2345.440 kb on + strand, within Pf1N1B4_2055 at 2345.441 kb on - strand, within Pf1N1B4_2055 at 2345.441 kb on - strand, within Pf1N1B4_2055 at 2345.441 kb on - strand, within Pf1N1B4_2055 at 2345.441 kb on - strand, within Pf1N1B4_2055 at 2345.441 kb on - strand, within Pf1N1B4_2055 at 2345.443 kb on + strand, within Pf1N1B4_2055  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 2,343,026 -  Pf1N1B4_2053 0.77  -0.0 2,343,026 -  Pf1N1B4_2053 0.77  -0.0 2,343,381 -  Pf1N1B4_2053 0.86  -0.0 2,343,381 -  Pf1N1B4_2053 0.86  -0.0 2,343,381 -  Pf1N1B4_2053 0.86  -0.0 2,343,577 +  -0.0 2,343,577 +  -0.0 2,343,577 +  -0.0 2,343,577 +  -0.0 2,343,577 +  -0.0 2,343,578 -  -0.0 2,343,578 -  -0.0 2,343,578 -  -2.6 2,343,707 +  -0.0 2,343,707 +  -1.6 2,343,707 +  -1.8 2,343,708 -  -1.8 2,343,708 -  -0.0 2,343,708 -  -0.0 2,344,111 +  Pf1N1B4_2054 0.20  -2.3 2,344,111 +  Pf1N1B4_2054 0.20  +1.0 2,344,112 -  Pf1N1B4_2054 0.20  -2.2 2,344,112 -  Pf1N1B4_2054 0.20  -0.0 2,344,112 -  Pf1N1B4_2054 0.20  -0.0 2,344,453 +  -0.0 2,344,453 +  -0.0 2,344,454 -  -1.9 2,344,624 +  -1.2 2,344,624 +  -0.0 2,344,624 +  -0.0 2,344,625 -  -1.4 2,344,625 -  -1.1 2,344,729 +  Pf1N1B4_2055 0.13  -0.0 2,344,729 +  Pf1N1B4_2055 0.13  -0.0 2,344,729 +  Pf1N1B4_2055 0.13  -0.0 2,344,729 +  -2.1 2,344,729 +  Pf1N1B4_2055 0.13  -0.7 2,344,729 +  Pf1N1B4_2055 0.13  -1.2 2,344,729 +  Pf1N1B4_2055 0.13  -0.0 2,344,729 +  Pf1N1B4_2055 0.13  -0.0 2,344,729 +  Pf1N1B4_2055 0.13  -0.0 2,344,729 +  Pf1N1B4_2055 0.13  -0.0 2,344,729 +  Pf1N1B4_2055 0.13  -0.0 2,344,729 +  Pf1N1B4_2055 0.13  -0.0 2,344,729 +  Pf1N1B4_2055 0.13  -0.0 2,344,729 +  Pf1N1B4_2055 0.13  -0.0 2,344,730 -  Pf1N1B4_2055 0.13  -0.0 2,344,730 -  Pf1N1B4_2055 0.13  -0.0 2,344,730 -  Pf1N1B4_2055 0.13  -0.0 2,344,730 -  Pf1N1B4_2055 0.13  -1.6 2,344,730 -  -2.1 2,344,730 -  Pf1N1B4_2055 0.13  -0.8 2,344,918 +  Pf1N1B4_2055 0.23  -0.0 2,344,918 +  Pf1N1B4_2055 0.23  -2.4 2,344,918 +  Pf1N1B4_2055 0.23  -2.9 2,344,918 +  Pf1N1B4_2055 0.23  -0.0 2,344,918 +  Pf1N1B4_2055 0.23  -0.0 2,344,918 +  Pf1N1B4_2055 0.23  -0.0 2,344,918 +  Pf1N1B4_2055 0.23  -0.0 2,344,918 +  Pf1N1B4_2055 0.23  -0.0 2,344,918 +  Pf1N1B4_2055 0.23  -0.0 2,344,918 +  Pf1N1B4_2055 0.23  -0.0 2,344,919 -  Pf1N1B4_2055 0.23  -2.9 2,344,919 -  Pf1N1B4_2055 0.23  -0.0 2,345,060 -  Pf1N1B4_2055 0.30  -0.0 2,345,230 +  Pf1N1B4_2055 0.39  -0.0 2,345,230 +  Pf1N1B4_2055 0.39  -1.6 2,345,230 +  Pf1N1B4_2055 0.39  -0.0 2,345,321 +  Pf1N1B4_2055 0.43  -0.0 2,345,351 -  Pf1N1B4_2055 0.45  -0.0 2,345,440 +  Pf1N1B4_2055 0.50  -2.3 2,345,440 +  Pf1N1B4_2055 0.50  -0.0 2,345,440 +  Pf1N1B4_2055 0.50  -0.0 2,345,441 -  Pf1N1B4_2055 0.50  -0.0 2,345,441 -  Pf1N1B4_2055 0.50  -0.0 2,345,441 -  Pf1N1B4_2055 0.50  -0.0 2,345,441 -  Pf1N1B4_2055 0.50  -2.1 2,345,441 -  Pf1N1B4_2055 0.50  -0.0 2,345,443 +  Pf1N1B4_2055 0.50  -0.0 
Or see this region's nucleotide sequence