Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_2034 and Pf1N1B4_2035 are separated by 234 nucleotides Pf1N1B4_2035 and Pf1N1B4_2036 are separated by 15 nucleotides 
        Pf1N1B4_2034 - Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1), at 2,320,811 to 2,321,809 
        _2034 
         
        
        Pf1N1B4_2035 - Phosphate transport system permease protein PstC (TC 3.A.1.7.1), at 2,322,044 to 2,324,332 
        _2035 
         
        
        Pf1N1B4_2036 - Phosphate transport system permease protein PstA (TC 3.A.1.7.1), at 2,324,348 to 2,326,018 
        _2036 
         Position (kb) 2322 
2323 
2324 
2325 Strain fitness (log2 ratio) -5 
-4 
-3 
-2 
-1 
0 
1 at 2321.726 kb on + strand at 2321.726 kb on + strand at 2321.727 kb on - strand at 2321.796 kb on + strand at 2321.796 kb on + strand at 2321.796 kb on + strand at 2321.796 kb on + strand at 2321.797 kb on - strand at 2321.797 kb on - strand at 2321.797 kb on - strand at 2321.797 kb on - strand at 2321.797 kb on - strand at 2321.797 kb on - strand at 2321.797 kb on - strand at 2321.797 kb on - strand at 2321.799 kb on + strand at 2321.799 kb on + strand at 2321.799 kb on + strand at 2321.799 kb on + strand at 2321.815 kb on + strand at 2321.897 kb on + strand at 2321.897 kb on + strand at 2321.897 kb on + strand at 2321.897 kb on + strand at 2321.897 kb on + strand at 2321.898 kb on - strand at 2321.898 kb on - strand at 2321.898 kb on - strand at 2321.898 kb on - strand at 2321.929 kb on - strand at 2322.027 kb on + strand at 2322.027 kb on + strand at 2322.110 kb on - strand at 2322.590 kb on + strand, within Pf1N1B4_2035 at 2322.839 kb on + strand, within Pf1N1B4_2035 at 2323.343 kb on + strand, within Pf1N1B4_2035 at 2323.344 kb on - strand, within Pf1N1B4_2035 at 2323.358 kb on + strand, within Pf1N1B4_2035 at 2323.358 kb on + strand, within Pf1N1B4_2035 at 2323.448 kb on + strand, within Pf1N1B4_2035 at 2323.502 kb on + strand, within Pf1N1B4_2035 at 2323.503 kb on - strand, within Pf1N1B4_2035 at 2323.610 kb on + strand, within Pf1N1B4_2035 at 2323.787 kb on + strand, within Pf1N1B4_2035 at 2323.787 kb on + strand, within Pf1N1B4_2035 at 2323.799 kb on + strand, within Pf1N1B4_2035 at 2323.865 kb on + strand, within Pf1N1B4_2035 at 2323.865 kb on + strand, within Pf1N1B4_2035 at 2323.865 kb on + strand, within Pf1N1B4_2035 at 2323.865 kb on + strand, within Pf1N1B4_2035 at 2323.866 kb on - strand, within Pf1N1B4_2035 at 2323.928 kb on + strand, within Pf1N1B4_2035 at 2323.929 kb on - strand, within Pf1N1B4_2035 at 2323.929 kb on - strand, within Pf1N1B4_2035 at 2324.318 kb on + strand at 2324.330 kb on + strand at 2324.339 kb on + strand at 2324.339 kb on + strand at 2324.339 kb on + strand at 2324.339 kb on + strand at 2324.339 kb on + strand at 2324.339 kb on + strand at 2325.194 kb on + strand, within Pf1N1B4_2036 at 2325.195 kb on - strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 2,321,726 +  -0.0 2,321,726 +  -0.0 2,321,727 -  -0.0 2,321,796 +  -0.0 2,321,796 +  -1.9 2,321,796 +  +1.0 2,321,796 +  -2.2 2,321,797 -  -0.0 2,321,797 -  -2.7 2,321,797 -  -0.0 2,321,797 -  -0.5 2,321,797 -  -0.0 2,321,797 -  -0.0 2,321,797 -  -0.0 2,321,797 -  -0.0 2,321,799 +  -1.7 2,321,799 +  -0.0 2,321,799 +  -0.0 2,321,799 +  -1.4 2,321,815 +  -0.0 2,321,897 +  -2.6 2,321,897 +  -0.0 2,321,897 +  -0.0 2,321,897 +  -0.0 2,321,897 +  -0.0 2,321,898 -  -2.3 2,321,898 -  -0.0 2,321,898 -  -0.0 2,321,898 -  -0.0 2,321,929 -  -0.0 2,322,027 +  -2.3 2,322,027 +  -0.0 2,322,110 -  -2.3 2,322,590 +  Pf1N1B4_2035 0.24  -0.0 2,322,839 +  Pf1N1B4_2035 0.35  -2.1 2,323,343 +  Pf1N1B4_2035 0.57  -0.0 2,323,344 -  Pf1N1B4_2035 0.57  -1.8 2,323,358 +  Pf1N1B4_2035 0.57  -0.0 2,323,358 +  Pf1N1B4_2035 0.57  -0.0 2,323,448 +  Pf1N1B4_2035 0.61  -0.0 2,323,502 +  Pf1N1B4_2035 0.64  -1.9 2,323,503 -  Pf1N1B4_2035 0.64  -3.9 2,323,610 +  Pf1N1B4_2035 0.68  -2.3 2,323,787 +  Pf1N1B4_2035 0.76  -0.0 2,323,787 +  Pf1N1B4_2035 0.76  -0.0 2,323,799 +  Pf1N1B4_2035 0.77  -0.0 2,323,865 +  Pf1N1B4_2035 0.80  +1.0 2,323,865 +  Pf1N1B4_2035 0.80  -0.0 2,323,865 +  Pf1N1B4_2035 0.80  -2.0 2,323,865 +  Pf1N1B4_2035 0.80  -0.0 2,323,866 -  Pf1N1B4_2035 0.80  -2.0 2,323,928 +  Pf1N1B4_2035 0.82  -3.8 2,323,929 -  Pf1N1B4_2035 0.82  -3.0 2,323,929 -  Pf1N1B4_2035 0.82  -0.0 2,324,318 +  -0.0 2,324,330 +  -4.8 2,324,339 +  -1.6 2,324,339 +  -0.0 2,324,339 +  -0.0 2,324,339 +  -0.0 2,324,339 +  -0.8 2,324,339 +  -0.0 2,325,194 +  Pf1N1B4_2036 0.51  -3.1 2,325,195 -  -0.0 
Or see this region's nucleotide sequence