Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1994 and Pf1N1B4_1995 overlap by 1 nucleotides Pf1N1B4_1995 and Pf1N1B4_1996 are separated by 4 nucleotides Pf1N1B4_1996 and Pf1N1B4_1997 are separated by 32 nucleotides Pf1N1B4_1997 and Pf1N1B4_1998 are separated by 59 nucleotides
Pf1N1B4_1994 - Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family, at 2,278,336 to 2,279,544
_1994
Pf1N1B4_1995 - FIG00954044: hypothetical protein, at 2,279,544 to 2,280,194
_1995
Pf1N1B4_1996 - NADH:ubiquinone oxidoreductase 49 kD subunit 7, at 2,280,199 to 2,280,546
_1996
Pf1N1B4_1997 - Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family, at 2,280,579 to 2,280,878
_1997
Pf1N1B4_1998 - Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family, at 2,280,938 to 2,281,549
_1998
Position (kb)
2279
2280
2281 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2278.712 kb on + strand, within Pf1N1B4_1994 at 2278.712 kb on + strand, within Pf1N1B4_1994 at 2278.712 kb on + strand, within Pf1N1B4_1994 at 2278.713 kb on - strand, within Pf1N1B4_1994 at 2278.721 kb on + strand, within Pf1N1B4_1994 at 2278.769 kb on - strand, within Pf1N1B4_1994 at 2278.769 kb on - strand, within Pf1N1B4_1994 at 2279.096 kb on - strand, within Pf1N1B4_1994 at 2279.206 kb on + strand, within Pf1N1B4_1994 at 2279.206 kb on + strand, within Pf1N1B4_1994 at 2279.207 kb on - strand, within Pf1N1B4_1994 at 2279.207 kb on - strand, within Pf1N1B4_1994 at 2279.498 kb on + strand at 2279.498 kb on + strand at 2279.499 kb on - strand at 2279.597 kb on + strand at 2279.598 kb on - strand at 2279.777 kb on - strand, within Pf1N1B4_1995 at 2279.958 kb on + strand, within Pf1N1B4_1995 at 2279.958 kb on + strand, within Pf1N1B4_1995 at 2279.958 kb on + strand, within Pf1N1B4_1995 at 2279.959 kb on - strand, within Pf1N1B4_1995 at 2280.013 kb on + strand, within Pf1N1B4_1995 at 2280.013 kb on + strand, within Pf1N1B4_1995 at 2280.014 kb on - strand, within Pf1N1B4_1995 at 2280.014 kb on - strand, within Pf1N1B4_1995 at 2280.014 kb on - strand, within Pf1N1B4_1995 at 2280.017 kb on + strand, within Pf1N1B4_1995 at 2280.017 kb on + strand, within Pf1N1B4_1995 at 2280.017 kb on + strand, within Pf1N1B4_1995 at 2280.018 kb on - strand, within Pf1N1B4_1995 at 2280.018 kb on - strand, within Pf1N1B4_1995 at 2280.251 kb on + strand, within Pf1N1B4_1996 at 2280.252 kb on - strand, within Pf1N1B4_1996 at 2280.275 kb on + strand, within Pf1N1B4_1996 at 2280.278 kb on + strand, within Pf1N1B4_1996 at 2280.278 kb on + strand, within Pf1N1B4_1996 at 2280.278 kb on + strand, within Pf1N1B4_1996 at 2280.278 kb on + strand, within Pf1N1B4_1996 at 2280.279 kb on - strand, within Pf1N1B4_1996 at 2280.279 kb on - strand, within Pf1N1B4_1996 at 2280.279 kb on - strand, within Pf1N1B4_1996 at 2280.303 kb on + strand, within Pf1N1B4_1996 at 2280.303 kb on + strand, within Pf1N1B4_1996 at 2280.304 kb on - strand, within Pf1N1B4_1996 at 2280.362 kb on + strand, within Pf1N1B4_1996 at 2280.362 kb on + strand, within Pf1N1B4_1996 at 2280.362 kb on + strand, within Pf1N1B4_1996 at 2280.363 kb on - strand, within Pf1N1B4_1996 at 2280.363 kb on - strand, within Pf1N1B4_1996 at 2280.556 kb on + strand at 2280.556 kb on + strand at 2280.556 kb on + strand at 2280.894 kb on + strand at 2280.894 kb on + strand at 2280.894 kb on + strand at 2281.112 kb on + strand, within Pf1N1B4_1998 at 2281.113 kb on - strand, within Pf1N1B4_1998 at 2281.113 kb on - strand, within Pf1N1B4_1998 at 2281.113 kb on - strand, within Pf1N1B4_1998
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 2,278,712 + Pf1N1B4_1994 0.31 -1.4 2,278,712 + Pf1N1B4_1994 0.31 -0.0 2,278,712 + Pf1N1B4_1994 0.31 -1.4 2,278,713 - Pf1N1B4_1994 0.31 -0.0 2,278,721 + Pf1N1B4_1994 0.32 -0.0 2,278,769 - Pf1N1B4_1994 0.36 -2.8 2,278,769 - Pf1N1B4_1994 0.36 -0.0 2,279,096 - Pf1N1B4_1994 0.63 -0.0 2,279,206 + Pf1N1B4_1994 0.72 -0.0 2,279,206 + Pf1N1B4_1994 0.72 -0.0 2,279,207 - Pf1N1B4_1994 0.72 -2.5 2,279,207 - Pf1N1B4_1994 0.72 -2.8 2,279,498 + -0.0 2,279,498 + -0.0 2,279,499 - -1.1 2,279,597 + -0.0 2,279,598 - -0.0 2,279,777 - Pf1N1B4_1995 0.36 -0.0 2,279,958 + Pf1N1B4_1995 0.64 -0.0 2,279,958 + Pf1N1B4_1995 0.64 -0.0 2,279,958 + Pf1N1B4_1995 0.64 -1.8 2,279,959 - Pf1N1B4_1995 0.64 -2.4 2,280,013 + Pf1N1B4_1995 0.72 -0.4 2,280,013 + Pf1N1B4_1995 0.72 -0.0 2,280,014 - Pf1N1B4_1995 0.72 -0.0 2,280,014 - Pf1N1B4_1995 0.72 -0.0 2,280,014 - Pf1N1B4_1995 0.72 -1.6 2,280,017 + Pf1N1B4_1995 0.73 -1.0 2,280,017 + Pf1N1B4_1995 0.73 +1.0 2,280,017 + Pf1N1B4_1995 0.73 -0.0 2,280,018 - Pf1N1B4_1995 0.73 -0.0 2,280,018 - Pf1N1B4_1995 0.73 -5.6 2,280,251 + Pf1N1B4_1996 0.15 -2.7 2,280,252 - Pf1N1B4_1996 0.15 -1.2 2,280,275 + Pf1N1B4_1996 0.22 -0.0 2,280,278 + Pf1N1B4_1996 0.23 -0.0 2,280,278 + Pf1N1B4_1996 0.23 -0.0 2,280,278 + Pf1N1B4_1996 0.23 -0.0 2,280,278 + Pf1N1B4_1996 0.23 -0.0 2,280,279 - Pf1N1B4_1996 0.23 -2.9 2,280,279 - Pf1N1B4_1996 0.23 -0.0 2,280,279 - Pf1N1B4_1996 0.23 -0.1 2,280,303 + Pf1N1B4_1996 0.30 -1.3 2,280,303 + Pf1N1B4_1996 0.30 -0.0 2,280,304 - Pf1N1B4_1996 0.30 -0.0 2,280,362 + Pf1N1B4_1996 0.47 -0.0 2,280,362 + Pf1N1B4_1996 0.47 -0.7 2,280,362 + Pf1N1B4_1996 0.47 -0.0 2,280,363 - Pf1N1B4_1996 0.47 -0.0 2,280,363 - Pf1N1B4_1996 0.47 -0.0 2,280,556 + -0.0 2,280,556 + -1.2 2,280,556 + -2.2 2,280,894 + -1.8 2,280,894 + -0.0 2,280,894 + -0.0 2,281,112 + Pf1N1B4_1998 0.28 -0.0 2,281,113 - Pf1N1B4_1998 0.29 -0.0 2,281,113 - Pf1N1B4_1998 0.29 -1.0 2,281,113 - Pf1N1B4_1998 0.29 -0.0
Or see this region's nucleotide sequence