Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1991 and Pf1N1B4_1992 overlap by 4 nucleotides Pf1N1B4_1992 and Pf1N1B4_1993 are separated by 127 nucleotides Pf1N1B4_1993 and Pf1N1B4_1994 are separated by 28 nucleotides Pf1N1B4_1994 and Pf1N1B4_1995 overlap by 1 nucleotides Pf1N1B4_1995 and Pf1N1B4_1996 are separated by 4 nucleotides
Pf1N1B4_1991 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 2,276,193 to 2,277,401
_1991
Pf1N1B4_1992 - Glutamine synthetase adenylyltransferase, at 2,277,398 to 2,277,775
_1992
Pf1N1B4_1993 - C4-type zinc finger protein, DksA/TraR family, at 2,277,903 to 2,278,307
_1993
Pf1N1B4_1994 - Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family, at 2,278,336 to 2,279,544
_1994
Pf1N1B4_1995 - FIG00954044: hypothetical protein, at 2,279,544 to 2,280,194
_1995
Pf1N1B4_1996 - NADH:ubiquinone oxidoreductase 49 kD subunit 7, at 2,280,199 to 2,280,546
_1996
Position (kb)
2278
2279
2280 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2277.360 kb on + strand at 2277.360 kb on + strand at 2277.360 kb on + strand at 2277.361 kb on - strand at 2277.361 kb on - strand at 2277.493 kb on - strand, within Pf1N1B4_1992 at 2277.493 kb on - strand, within Pf1N1B4_1992 at 2277.588 kb on - strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.636 kb on + strand, within Pf1N1B4_1992 at 2277.636 kb on + strand, within Pf1N1B4_1992 at 2277.656 kb on + strand, within Pf1N1B4_1992 at 2277.656 kb on + strand, within Pf1N1B4_1992 at 2277.656 kb on + strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.802 kb on + strand at 2277.802 kb on + strand at 2277.803 kb on - strand at 2277.803 kb on - strand at 2277.828 kb on + strand at 2277.829 kb on - strand at 2277.942 kb on + strand at 2277.942 kb on + strand at 2277.943 kb on - strand at 2277.943 kb on - strand at 2277.943 kb on - strand at 2277.943 kb on - strand at 2278.253 kb on + strand, within Pf1N1B4_1993 at 2278.253 kb on + strand, within Pf1N1B4_1993 at 2278.330 kb on + strand at 2278.331 kb on - strand at 2278.331 kb on - strand at 2278.393 kb on - strand at 2278.401 kb on + strand at 2278.401 kb on + strand at 2278.402 kb on - strand at 2278.405 kb on + strand at 2278.405 kb on + strand at 2278.405 kb on + strand at 2278.405 kb on + strand at 2278.410 kb on - strand at 2278.413 kb on - strand at 2278.413 kb on - strand at 2278.712 kb on + strand, within Pf1N1B4_1994 at 2278.712 kb on + strand, within Pf1N1B4_1994 at 2278.712 kb on + strand, within Pf1N1B4_1994 at 2278.713 kb on - strand, within Pf1N1B4_1994 at 2278.721 kb on + strand, within Pf1N1B4_1994 at 2278.769 kb on - strand, within Pf1N1B4_1994 at 2278.769 kb on - strand, within Pf1N1B4_1994 at 2279.096 kb on - strand, within Pf1N1B4_1994 at 2279.206 kb on + strand, within Pf1N1B4_1994 at 2279.206 kb on + strand, within Pf1N1B4_1994 at 2279.207 kb on - strand, within Pf1N1B4_1994 at 2279.207 kb on - strand, within Pf1N1B4_1994 at 2279.498 kb on + strand at 2279.498 kb on + strand at 2279.499 kb on - strand at 2279.597 kb on + strand at 2279.598 kb on - strand at 2279.777 kb on - strand, within Pf1N1B4_1995 at 2279.958 kb on + strand, within Pf1N1B4_1995 at 2279.958 kb on + strand, within Pf1N1B4_1995 at 2279.958 kb on + strand, within Pf1N1B4_1995 at 2279.959 kb on - strand, within Pf1N1B4_1995 at 2280.013 kb on + strand, within Pf1N1B4_1995 at 2280.013 kb on + strand, within Pf1N1B4_1995 at 2280.014 kb on - strand, within Pf1N1B4_1995 at 2280.014 kb on - strand, within Pf1N1B4_1995 at 2280.014 kb on - strand, within Pf1N1B4_1995 at 2280.017 kb on + strand, within Pf1N1B4_1995 at 2280.017 kb on + strand, within Pf1N1B4_1995 at 2280.017 kb on + strand, within Pf1N1B4_1995 at 2280.018 kb on - strand, within Pf1N1B4_1995 at 2280.018 kb on - strand, within Pf1N1B4_1995 at 2280.251 kb on + strand, within Pf1N1B4_1996 at 2280.252 kb on - strand, within Pf1N1B4_1996 at 2280.275 kb on + strand, within Pf1N1B4_1996 at 2280.278 kb on + strand, within Pf1N1B4_1996 at 2280.278 kb on + strand, within Pf1N1B4_1996 at 2280.278 kb on + strand, within Pf1N1B4_1996 at 2280.278 kb on + strand, within Pf1N1B4_1996 at 2280.279 kb on - strand, within Pf1N1B4_1996 at 2280.279 kb on - strand, within Pf1N1B4_1996 at 2280.279 kb on - strand, within Pf1N1B4_1996 at 2280.303 kb on + strand, within Pf1N1B4_1996 at 2280.303 kb on + strand, within Pf1N1B4_1996 at 2280.304 kb on - strand, within Pf1N1B4_1996 at 2280.362 kb on + strand, within Pf1N1B4_1996 at 2280.362 kb on + strand, within Pf1N1B4_1996 at 2280.362 kb on + strand, within Pf1N1B4_1996 at 2280.363 kb on - strand, within Pf1N1B4_1996 at 2280.363 kb on - strand, within Pf1N1B4_1996
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 2,277,360 + -1.7 2,277,360 + -0.0 2,277,360 + -1.8 2,277,361 - -1.4 2,277,361 - -4.6 2,277,493 - Pf1N1B4_1992 0.25 -0.0 2,277,493 - Pf1N1B4_1992 0.25 -3.3 2,277,588 - Pf1N1B4_1992 0.50 -0.0 2,277,606 + Pf1N1B4_1992 0.55 -2.5 2,277,606 + Pf1N1B4_1992 0.55 -0.0 2,277,606 + Pf1N1B4_1992 0.55 -0.0 2,277,606 + Pf1N1B4_1992 0.55 -0.0 2,277,606 + Pf1N1B4_1992 0.55 -0.0 2,277,607 - Pf1N1B4_1992 0.55 -0.0 2,277,607 - Pf1N1B4_1992 0.55 -0.0 2,277,607 - Pf1N1B4_1992 0.55 -0.0 2,277,607 - Pf1N1B4_1992 0.55 -0.0 2,277,607 - Pf1N1B4_1992 0.55 -2.0 2,277,607 - Pf1N1B4_1992 0.55 -0.0 2,277,607 - Pf1N1B4_1992 0.55 -0.4 2,277,607 - Pf1N1B4_1992 0.55 -1.8 2,277,607 - Pf1N1B4_1992 0.55 -0.0 2,277,607 - Pf1N1B4_1992 0.55 -2.0 2,277,636 + Pf1N1B4_1992 0.63 -0.0 2,277,636 + Pf1N1B4_1992 0.63 -0.0 2,277,656 + Pf1N1B4_1992 0.68 -0.0 2,277,656 + Pf1N1B4_1992 0.68 -0.0 2,277,656 + Pf1N1B4_1992 0.68 -0.0 2,277,657 - Pf1N1B4_1992 0.69 -0.0 2,277,657 - Pf1N1B4_1992 0.69 -0.0 2,277,657 - Pf1N1B4_1992 0.69 -0.0 2,277,657 - Pf1N1B4_1992 0.69 -0.0 2,277,657 - Pf1N1B4_1992 0.69 -0.0 2,277,802 + -0.0 2,277,802 + -0.0 2,277,803 - -0.0 2,277,803 - -0.8 2,277,828 + -2.3 2,277,829 - -2.2 2,277,942 + -0.0 2,277,942 + -0.0 2,277,943 - -0.0 2,277,943 - -0.0 2,277,943 - -0.0 2,277,943 - -0.0 2,278,253 + Pf1N1B4_1993 0.86 -0.0 2,278,253 + Pf1N1B4_1993 0.86 +0.1 2,278,330 + -0.0 2,278,331 - -0.0 2,278,331 - -0.0 2,278,393 - -0.0 2,278,401 + -0.0 2,278,401 + -0.0 2,278,402 - -0.0 2,278,405 + -2.1 2,278,405 + -0.0 2,278,405 + -0.0 2,278,405 + -1.3 2,278,410 - -0.0 2,278,413 - -0.0 2,278,413 - -0.0 2,278,712 + Pf1N1B4_1994 0.31 -1.4 2,278,712 + Pf1N1B4_1994 0.31 -0.0 2,278,712 + Pf1N1B4_1994 0.31 -1.4 2,278,713 - Pf1N1B4_1994 0.31 -0.0 2,278,721 + Pf1N1B4_1994 0.32 -0.0 2,278,769 - Pf1N1B4_1994 0.36 -2.8 2,278,769 - Pf1N1B4_1994 0.36 -0.0 2,279,096 - Pf1N1B4_1994 0.63 -0.0 2,279,206 + Pf1N1B4_1994 0.72 -0.0 2,279,206 + Pf1N1B4_1994 0.72 -0.0 2,279,207 - Pf1N1B4_1994 0.72 -2.5 2,279,207 - Pf1N1B4_1994 0.72 -2.8 2,279,498 + -0.0 2,279,498 + -0.0 2,279,499 - -1.1 2,279,597 + -0.0 2,279,598 - -0.0 2,279,777 - Pf1N1B4_1995 0.36 -0.0 2,279,958 + Pf1N1B4_1995 0.64 -0.0 2,279,958 + Pf1N1B4_1995 0.64 -0.0 2,279,958 + Pf1N1B4_1995 0.64 -1.8 2,279,959 - Pf1N1B4_1995 0.64 -2.4 2,280,013 + Pf1N1B4_1995 0.72 -0.4 2,280,013 + Pf1N1B4_1995 0.72 -0.0 2,280,014 - Pf1N1B4_1995 0.72 -0.0 2,280,014 - Pf1N1B4_1995 0.72 -0.0 2,280,014 - Pf1N1B4_1995 0.72 -1.6 2,280,017 + Pf1N1B4_1995 0.73 -1.0 2,280,017 + Pf1N1B4_1995 0.73 +1.0 2,280,017 + Pf1N1B4_1995 0.73 -0.0 2,280,018 - Pf1N1B4_1995 0.73 -0.0 2,280,018 - Pf1N1B4_1995 0.73 -5.6 2,280,251 + Pf1N1B4_1996 0.15 -2.7 2,280,252 - Pf1N1B4_1996 0.15 -1.2 2,280,275 + Pf1N1B4_1996 0.22 -0.0 2,280,278 + Pf1N1B4_1996 0.23 -0.0 2,280,278 + Pf1N1B4_1996 0.23 -0.0 2,280,278 + Pf1N1B4_1996 0.23 -0.0 2,280,278 + Pf1N1B4_1996 0.23 -0.0 2,280,279 - Pf1N1B4_1996 0.23 -2.9 2,280,279 - Pf1N1B4_1996 0.23 -0.0 2,280,279 - Pf1N1B4_1996 0.23 -0.1 2,280,303 + Pf1N1B4_1996 0.30 -1.3 2,280,303 + Pf1N1B4_1996 0.30 -0.0 2,280,304 - Pf1N1B4_1996 0.30 -0.0 2,280,362 + Pf1N1B4_1996 0.47 -0.0 2,280,362 + Pf1N1B4_1996 0.47 -0.7 2,280,362 + Pf1N1B4_1996 0.47 -0.0 2,280,363 - Pf1N1B4_1996 0.47 -0.0 2,280,363 - Pf1N1B4_1996 0.47 -0.0
Or see this region's nucleotide sequence