Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1975

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
2,257,224	-		Pf1N1B4_1973	0.90	-1.6
2,257,227	+		Pf1N1B4_1973	0.90	-2.4
2,257,227	+		Pf1N1B4_1973	0.90	+0.0
2,257,227	+		Pf1N1B4_1973	0.90	-0.7
2,257,227	+		Pf1N1B4_1973	0.90	+0.0
2,257,228	-		Pf1N1B4_1973	0.90	-2.2
2,257,228	-		Pf1N1B4_1973	0.90	+0.0
2,257,241	+				+0.0
2,257,241	+				-1.6
2,257,241	+				+0.0
2,257,241	+				-1.4
2,257,241	+				-2.0
2,257,241	+				-1.9
2,257,241	+				-1.2
2,257,241	+				+0.0
2,257,241	+				+0.0
2,257,241	+				-1.3
2,257,241	+				+0.0
2,257,241	+				+0.0
2,257,241	+				+0.0
2,257,242	-				-3.1
2,257,303	+				+0.0
2,257,310	+				-3.1
2,257,310	+				-1.6
2,257,310	+				+0.0
2,257,311	-				-2.1
2,257,311	-				-2.4
2,257,311	-				-0.5
2,257,311	-				+0.0
2,257,311	-				+0.0
2,257,355	+				+0.0
2,257,355	+				+0.0
2,257,394	-				-2.4
2,257,410	+				-1.2
2,257,411	-				+0.0
2,257,432	+				+0.0
2,257,432	+				+0.0
2,257,433	-				+0.0
2,257,433	-				+0.0
2,257,578	+		Pf1N1B4_1974	0.21	-1.9
2,257,941	-		Pf1N1B4_1974	0.78	-1.6
2,258,034	+				+0.0
2,258,034	+				+0.0
2,258,035	-				-1.3
2,258,035	-				+0.0
2,258,035	-				-1.5
2,258,035	-				+0.0
2,258,117	+				-0.0
2,258,117	+				-0.0
2,258,117	+				-0.0
2,258,117	+				-0.0
2,258,118	-				-0.0
2,258,118	-				-1.8
2,258,118	-				-0.0
2,258,118	-				-0.0
2,258,118	-				-1.8
2,258,179	-				-1.6
2,258,575	+		Pf1N1B4_1975	0.74	-1.8
2,258,576	-		Pf1N1B4_1975	0.74	-0.0
2,258,576	-		Pf1N1B4_1975	0.74	-3.1
2,258,576	-		Pf1N1B4_1975	0.74	-0.0
2,258,695	+				-0.0
2,258,695	+				-1.4
2,258,695	+				-1.8
2,258,821	+				-0.0
2,258,821	+				-1.1
2,258,821	+				-0.0
2,258,821	+				-0.0
2,258,821	+				-1.6
2,258,821	+				-1.4
2,258,821	+				-0.0
2,258,821	+				-0.0
2,258,822	-				-0.0
2,258,822	-				-2.0
2,258,822	-				-1.1
2,258,822	-				-0.0
2,258,822	-				-0.0
2,258,822	-				-0.0
2,258,822	-				-1.8
2,258,822	-				-0.0
2,259,024	+		Pf1N1B4_1976	0.15	-1.1
2,259,024	+		Pf1N1B4_1976	0.15	-0.0
2,259,024	+		Pf1N1B4_1976	0.15	-0.0
2,259,024	+				-0.0
2,259,025	-		Pf1N1B4_1976	0.15	-0.0
2,259,025	-		Pf1N1B4_1976	0.15	-0.0
2,259,025	-		Pf1N1B4_1976	0.15	-0.0
2,259,025	-		Pf1N1B4_1976	0.15	-0.0
2,259,025	-		Pf1N1B4_1976	0.15	-3.1
2,259,025	-		Pf1N1B4_1976	0.15	-0.0
2,259,098	+		Pf1N1B4_1976	0.20	-0.9
2,259,098	+		Pf1N1B4_1976	0.20	-1.0
2,259,100	+		Pf1N1B4_1976	0.20	+0.6
2,259,100	+		Pf1N1B4_1976	0.20	-0.0
2,259,100	+		Pf1N1B4_1976	0.20	-0.0
2,259,100	+		Pf1N1B4_1976	0.20	-0.6
2,259,101	-		Pf1N1B4_1976	0.20	-0.0
2,259,101	-		Pf1N1B4_1976	0.20	+1.5
2,259,101	-		Pf1N1B4_1976	0.20	-0.0
2,259,166	+		Pf1N1B4_1976	0.25	-1.0
2,259,166	+		Pf1N1B4_1976	0.25	-0.0
2,259,166	+		Pf1N1B4_1976	0.25	-0.0
2,259,166	+		Pf1N1B4_1976	0.25	-0.0
2,259,167	-		Pf1N1B4_1976	0.25	-0.0
2,259,190	+		Pf1N1B4_1976	0.26	-0.0
2,259,190	+		Pf1N1B4_1976	0.26	-0.0
2,259,190	+		Pf1N1B4_1976	0.26	+1.0
2,259,190	+		Pf1N1B4_1976	0.26	-0.0
2,259,191	-		Pf1N1B4_1976	0.26	-1.4
2,259,191	-		Pf1N1B4_1976	0.26	-0.9
2,259,191	-		Pf1N1B4_1976	0.26	-0.0
2,259,191	-		Pf1N1B4_1976	0.26	-0.0
2,259,191	-		Pf1N1B4_1976	0.26	-0.0
2,259,191	-		Pf1N1B4_1976	0.26	-2.0
2,259,389	+		Pf1N1B4_1976	0.41	-1.3
2,259,389	+		Pf1N1B4_1976	0.41	-1.5
2,259,390	-		Pf1N1B4_1976	0.41	-0.0
2,259,390	-		Pf1N1B4_1976	0.41	-0.0
2,259,390	-		Pf1N1B4_1976	0.41	-3.2
2,259,390	-		Pf1N1B4_1976	0.41	-2.1
2,259,391	+		Pf1N1B4_1976	0.41	-0.0
2,259,391	+		Pf1N1B4_1976	0.41	-0.0
2,259,391	+		Pf1N1B4_1976	0.41	-0.0
2,259,391	+		Pf1N1B4_1976	0.41	-0.0
2,259,391	+		Pf1N1B4_1976	0.41	-1.7
2,259,392	-		Pf1N1B4_1976	0.41	-1.1
2,259,421	+		Pf1N1B4_1976	0.43	-1.2
2,259,422	-		Pf1N1B4_1976	0.43	-0.0
2,259,422	-		Pf1N1B4_1976	0.43	-0.0
2,259,422	-		Pf1N1B4_1976	0.43	-0.0