Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_1624 and Pf1N1B4_1625 are separated by 99 nucleotides Pf1N1B4_1625 and Pf1N1B4_1626 are separated by 0 nucleotides Pf1N1B4_1626 and Pf1N1B4_1627 overlap by 1 nucleotides Pf1N1B4_1627 and Pf1N1B4_1628 are separated by 229 nucleotides 
        Pf1N1B4_1624 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) , at 1,868,509 to 1,869,246 
        _1624 
         
        
        Pf1N1B4_1625 - FIG00956267: hypothetical protein, at 1,869,346 to 1,869,603 
        _1625 
         
        
        Pf1N1B4_1626 - Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-), at 1,869,604 to 1,870,242 
        _1626 
         
        
        Pf1N1B4_1627 - Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19), at 1,870,242 to 1,870,877 
        _1627 
         
        
        Pf1N1B4_1628 - MFS permease, at 1,871,107 to 1,872,771 
        _1628 
         Position (kb) 1870 
1871 Strain fitness (log2 ratio) -4 
-3 
-2 
-1 
0 
1 at 1869.242 kb on - strand at 1869.242 kb on - strand at 1869.242 kb on - strand at 1869.242 kb on - strand at 1869.242 kb on - strand at 1869.242 kb on - strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.283 kb on - strand at 1869.283 kb on - strand at 1869.304 kb on + strand at 1869.347 kb on + strand at 1869.348 kb on - strand at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.435 kb on - strand, within Pf1N1B4_1625 at 1869.435 kb on - strand, within Pf1N1B4_1625 at 1869.435 kb on - strand, within Pf1N1B4_1625 at 1869.437 kb on + strand, within Pf1N1B4_1625 at 1869.438 kb on - strand, within Pf1N1B4_1625 at 1869.438 kb on - strand, within Pf1N1B4_1625 at 1869.606 kb on - strand at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.776 kb on + strand at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.779 kb on + strand, within Pf1N1B4_1626 at 1869.779 kb on + strand, within Pf1N1B4_1626 at 1869.779 kb on + strand at 1869.779 kb on + strand, within Pf1N1B4_1626 at 1869.779 kb on + strand, within Pf1N1B4_1626 at 1869.779 kb on + strand, within Pf1N1B4_1626 at 1869.779 kb on + strand, within Pf1N1B4_1626 at 1869.780 kb on - strand, within Pf1N1B4_1626 at 1869.780 kb on - strand, within Pf1N1B4_1626 at 1869.780 kb on - strand, within Pf1N1B4_1626 at 1869.780 kb on - strand, within Pf1N1B4_1626 at 1869.780 kb on - strand, within Pf1N1B4_1626 at 1869.795 kb on - strand, within Pf1N1B4_1626 at 1869.795 kb on - strand, within Pf1N1B4_1626 at 1869.795 kb on - strand, within Pf1N1B4_1626 at 1869.795 kb on - strand, within Pf1N1B4_1626 at 1869.795 kb on - strand, within Pf1N1B4_1626 at 1869.839 kb on - strand, within Pf1N1B4_1626 at 1869.839 kb on - strand, within Pf1N1B4_1626 at 1869.839 kb on - strand, within Pf1N1B4_1626 at 1870.466 kb on + strand, within Pf1N1B4_1627 at 1870.468 kb on + strand, within Pf1N1B4_1627 at 1870.468 kb on + strand, within Pf1N1B4_1627 at 1870.469 kb on - strand, within Pf1N1B4_1627 at 1870.469 kb on - strand, within Pf1N1B4_1627 at 1870.546 kb on + strand, within Pf1N1B4_1627 at 1870.546 kb on + strand, within Pf1N1B4_1627 at 1870.546 kb on + strand, within Pf1N1B4_1627 at 1870.547 kb on - strand, within Pf1N1B4_1627 at 1870.547 kb on - strand, within Pf1N1B4_1627 at 1870.547 kb on - strand, within Pf1N1B4_1627 at 1870.547 kb on - strand, within Pf1N1B4_1627 at 1870.731 kb on + strand, within Pf1N1B4_1627 at 1870.731 kb on + strand, within Pf1N1B4_1627 at 1870.823 kb on + strand at 1870.823 kb on + strand at 1870.823 kb on + strand at 1870.824 kb on - strand at 1870.824 kb on - strand at 1870.824 kb on - strand at 1870.824 kb on - strand at 1870.824 kb on - strand at 1870.880 kb on + strand at 1870.880 kb on + strand at 1870.880 kb on + strand at 1870.880 kb on + strand at 1870.880 kb on + strand at 1870.880 kb on + strand at 1870.880 kb on + strand at 1870.880 kb on + strand at 1870.881 kb on - strand at 1870.881 kb on - strand at 1870.881 kb on - strand at 1870.881 kb on - strand at 1870.895 kb on + strand at 1870.917 kb on + strand at 1870.917 kb on + strand at 1870.953 kb on - strand at 1870.953 kb on - strand at 1870.964 kb on + strand at 1870.964 kb on + strand at 1870.964 kb on + strand at 1870.964 kb on + strand at 1870.964 kb on + strand at 1870.964 kb on + strand at 1870.964 kb on + strand at 1870.965 kb on - strand at 1870.965 kb on - strand at 1870.965 kb on - strand at 1870.965 kb on - strand at 1870.965 kb on - strand at 1871.109 kb on - strand at 1871.303 kb on + strand, within Pf1N1B4_1628 at 1871.303 kb on + strand, within Pf1N1B4_1628 at 1871.304 kb on - strand, within Pf1N1B4_1628 at 1871.304 kb on - strand, within Pf1N1B4_1628 at 1871.304 kb on - strand, within Pf1N1B4_1628 at 1871.304 kb on - strand, within Pf1N1B4_1628 at 1871.372 kb on + strand, within Pf1N1B4_1628 at 1871.372 kb on + strand, within Pf1N1B4_1628 at 1871.372 kb on + strand, within Pf1N1B4_1628 at 1871.372 kb on + strand, within Pf1N1B4_1628 at 1871.373 kb on - strand, within Pf1N1B4_1628 at 1871.373 kb on - strand, within Pf1N1B4_1628 at 1871.373 kb on - strand, within Pf1N1B4_1628 at 1871.373 kb on - strand, within Pf1N1B4_1628 at 1871.373 kb on - strand, within Pf1N1B4_1628 at 1871.373 kb on - strand, within Pf1N1B4_1628 at 1871.373 kb on - strand, within Pf1N1B4_1628 at 1871.373 kb on - strand, within Pf1N1B4_1628 at 1871.373 kb on - strand, within Pf1N1B4_1628 at 1871.388 kb on - strand, within Pf1N1B4_1628 at 1871.388 kb on - strand, within Pf1N1B4_1628 at 1871.388 kb on - strand, within Pf1N1B4_1628 at 1871.423 kb on + strand, within Pf1N1B4_1628 at 1871.423 kb on + strand, within Pf1N1B4_1628 at 1871.435 kb on + strand, within Pf1N1B4_1628 at 1871.435 kb on + strand, within Pf1N1B4_1628 at 1871.435 kb on + strand, within Pf1N1B4_1628 at 1871.435 kb on + strand, within Pf1N1B4_1628 at 1871.435 kb on + strand, within Pf1N1B4_1628 at 1871.436 kb on - strand, within Pf1N1B4_1628 at 1871.436 kb on - strand, within Pf1N1B4_1628 at 1871.436 kb on - strand, within Pf1N1B4_1628 at 1871.436 kb on - strand, within Pf1N1B4_1628 at 1871.436 kb on - strand, within Pf1N1B4_1628 at 1871.567 kb on + strand, within Pf1N1B4_1628 at 1871.568 kb on - strand, within Pf1N1B4_1628 at 1871.568 kb on - strand, within Pf1N1B4_1628 at 1871.646 kb on - strand, within Pf1N1B4_1628 at 1871.646 kb on - strand, within Pf1N1B4_1628 at 1871.671 kb on + strand, within Pf1N1B4_1628 at 1871.671 kb on + strand, within Pf1N1B4_1628 at 1871.672 kb on - strand, within Pf1N1B4_1628 at 1871.672 kb on - strand, within Pf1N1B4_1628 at 1871.672 kb on - strand, within Pf1N1B4_1628 at 1871.678 kb on + strand, within Pf1N1B4_1628 at 1871.678 kb on + strand, within Pf1N1B4_1628 at 1871.678 kb on + strand, within Pf1N1B4_1628 at 1871.679 kb on - strand, within Pf1N1B4_1628 at 1871.679 kb on - strand, within Pf1N1B4_1628 at 1871.679 kb on - strand, within Pf1N1B4_1628 at 1871.679 kb on - strand, within Pf1N1B4_1628 at 1871.679 kb on - strand, within Pf1N1B4_1628 at 1871.679 kb on - strand, within Pf1N1B4_1628 at 1871.679 kb on - strand, within Pf1N1B4_1628 at 1871.679 kb on - strand, within Pf1N1B4_1628 at 1871.679 kb on - strand, within Pf1N1B4_1628 at 1871.679 kb on - strand, within Pf1N1B4_1628 at 1871.679 kb on - strand, within Pf1N1B4_1628 at 1871.679 kb on - strand, within Pf1N1B4_1628 at 1871.699 kb on + strand, within Pf1N1B4_1628  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 1,869,242 -  -0.1 1,869,242 -  -0.1 1,869,242 -  -2.1 1,869,242 -  -2.0 1,869,242 -  -1.4 1,869,242 -  -0.1 1,869,282 +  -2.6 1,869,282 +  -1.6 1,869,282 +  -1.4 1,869,282 +  -1.5 1,869,282 +  -3.1 1,869,282 +  -0.1 1,869,282 +  -0.7 1,869,282 +  -3.8 1,869,282 +  -0.1 1,869,282 +  -1.7 1,869,282 +  -1.9 1,869,282 +  -1.3 1,869,282 +  -0.1 1,869,283 -  -1.6 1,869,283 -  -0.1 1,869,304 +  -0.1 1,869,347 +  -3.1 1,869,348 -  -0.1 1,869,434 +  Pf1N1B4_1625 0.34  -0.1 1,869,434 +  Pf1N1B4_1625 0.34  -0.2 1,869,434 +  Pf1N1B4_1625 0.34  -0.1 1,869,434 +  Pf1N1B4_1625 0.34  -1.8 1,869,434 +  Pf1N1B4_1625 0.34  -2.4 1,869,434 +  Pf1N1B4_1625 0.34  -0.1 1,869,434 +  Pf1N1B4_1625 0.34  -0.1 1,869,434 +  Pf1N1B4_1625 0.34  -0.1 1,869,435 -  Pf1N1B4_1625 0.34  -2.3 1,869,435 -  Pf1N1B4_1625 0.34  -0.1 1,869,435 -  Pf1N1B4_1625 0.34  -1.9 1,869,437 +  Pf1N1B4_1625 0.35  +0.9 1,869,438 -  Pf1N1B4_1625 0.36  -2.1 1,869,438 -  Pf1N1B4_1625 0.36  -0.1 1,869,606 -  -1.1 1,869,776 +  Pf1N1B4_1626 0.27  -0.0 1,869,776 +  Pf1N1B4_1626 0.27  -1.1 1,869,776 +  -2.9 1,869,776 +  Pf1N1B4_1626 0.27  -1.8 1,869,776 +  Pf1N1B4_1626 0.27  -0.9 1,869,776 +  Pf1N1B4_1626 0.27  -0.0 1,869,776 +  Pf1N1B4_1626 0.27  -0.0 1,869,776 +  Pf1N1B4_1626 0.27  -0.0 1,869,777 -  Pf1N1B4_1626 0.27  -0.0 1,869,777 -  Pf1N1B4_1626 0.27  -4.0 1,869,777 -  Pf1N1B4_1626 0.27  -0.0 1,869,777 -  Pf1N1B4_1626 0.27  -0.0 1,869,777 -  Pf1N1B4_1626 0.27  -3.2 1,869,777 -  Pf1N1B4_1626 0.27  -2.3 1,869,777 -  Pf1N1B4_1626 0.27  -0.0 1,869,777 -  Pf1N1B4_1626 0.27  -0.0 1,869,777 -  Pf1N1B4_1626 0.27  -0.0 1,869,777 -  Pf1N1B4_1626 0.27  -1.6 1,869,777 -  Pf1N1B4_1626 0.27  -0.0 1,869,777 -  Pf1N1B4_1626 0.27  -0.0 1,869,777 -  Pf1N1B4_1626 0.27  -0.0 1,869,779 +  Pf1N1B4_1626 0.27  -1.5 1,869,779 +  Pf1N1B4_1626 0.27  -1.3 1,869,779 +  -2.2 1,869,779 +  Pf1N1B4_1626 0.27  -1.9 1,869,779 +  Pf1N1B4_1626 0.27  -0.0 1,869,779 +  Pf1N1B4_1626 0.27  -1.4 1,869,779 +  Pf1N1B4_1626 0.27  -1.5 1,869,780 -  Pf1N1B4_1626 0.28  -1.3 1,869,780 -  Pf1N1B4_1626 0.28  -3.5 1,869,780 -  Pf1N1B4_1626 0.28  -0.0 1,869,780 -  Pf1N1B4_1626 0.28  -0.0 1,869,780 -  Pf1N1B4_1626 0.28  -2.9 1,869,795 -  Pf1N1B4_1626 0.30  -0.0 1,869,795 -  Pf1N1B4_1626 0.30  -0.0 1,869,795 -  Pf1N1B4_1626 0.30  -1.8 1,869,795 -  Pf1N1B4_1626 0.30  -1.9 1,869,795 -  Pf1N1B4_1626 0.30  -0.5 1,869,839 -  Pf1N1B4_1626 0.37  -2.5 1,869,839 -  Pf1N1B4_1626 0.37  -1.8 1,869,839 -  Pf1N1B4_1626 0.37  -0.0 1,870,466 +  Pf1N1B4_1627 0.35  -0.0 1,870,468 +  Pf1N1B4_1627 0.36  -1.3 1,870,468 +  Pf1N1B4_1627 0.36  -1.0 1,870,469 -  Pf1N1B4_1627 0.36  -0.0 1,870,469 -  Pf1N1B4_1627 0.36  -3.7 1,870,546 +  Pf1N1B4_1627 0.48  -2.2 1,870,546 +  Pf1N1B4_1627 0.48  -3.3 1,870,546 +  Pf1N1B4_1627 0.48  -1.9 1,870,547 -  Pf1N1B4_1627 0.48  -0.0 1,870,547 -  Pf1N1B4_1627 0.48  -0.0 1,870,547 -  Pf1N1B4_1627 0.48  -2.9 1,870,547 -  Pf1N1B4_1627 0.48  -0.0 1,870,731 +  Pf1N1B4_1627 0.77  -0.0 1,870,731 +  Pf1N1B4_1627 0.77  -1.8 1,870,823 +  -0.0 1,870,823 +  -0.0 1,870,823 +  -3.2 1,870,824 -  -1.8 1,870,824 -  -0.0 1,870,824 -  -2.4 1,870,824 -  -0.0 1,870,824 -  -0.0 1,870,880 +  -0.0 1,870,880 +  -0.0 1,870,880 +  -0.0 1,870,880 +  -0.0 1,870,880 +  -0.0 1,870,880 +  -0.0 1,870,880 +  -0.4 1,870,880 +  -1.6 1,870,881 -  -1.0 1,870,881 -  -0.0 1,870,881 -  -1.0 1,870,881 -  -0.0 1,870,895 +  -0.0 1,870,917 +  -0.0 1,870,917 +  -0.0 1,870,953 -  -0.0 1,870,953 -  -2.9 1,870,964 +  -0.0 1,870,964 +  -0.0 1,870,964 +  -0.0 1,870,964 +  -1.5 1,870,964 +  -0.0 1,870,964 +  -2.0 1,870,964 +  -2.4 1,870,965 -  -0.0 1,870,965 -  -0.0 1,870,965 -  -3.9 1,870,965 -  -0.0 1,870,965 -  -0.0 1,871,109 -  -0.0 1,871,303 +  Pf1N1B4_1628 0.12  -1.7 1,871,303 +  Pf1N1B4_1628 0.12  -0.0 1,871,304 -  Pf1N1B4_1628 0.12  -2.3 1,871,304 -  Pf1N1B4_1628 0.12  -1.3 1,871,304 -  Pf1N1B4_1628 0.12  -0.0 1,871,304 -  Pf1N1B4_1628 0.12  -0.0 1,871,372 +  Pf1N1B4_1628 0.16  -1.0 1,871,372 +  Pf1N1B4_1628 0.16  -0.0 1,871,372 +  Pf1N1B4_1628 0.16  -0.0 1,871,372 +  Pf1N1B4_1628 0.16  -0.0 1,871,373 -  Pf1N1B4_1628 0.16  -2.0 1,871,373 -  Pf1N1B4_1628 0.16  -0.0 1,871,373 -  Pf1N1B4_1628 0.16  -0.0 1,871,373 -  Pf1N1B4_1628 0.16  -0.0 1,871,373 -  Pf1N1B4_1628 0.16  -3.1 1,871,373 -  Pf1N1B4_1628 0.16  -0.0 1,871,373 -  Pf1N1B4_1628 0.16  -2.9 1,871,373 -  Pf1N1B4_1628 0.16  -0.0 1,871,373 -  Pf1N1B4_1628 0.16  -0.0 1,871,388 -  Pf1N1B4_1628 0.17  -2.2 1,871,388 -  Pf1N1B4_1628 0.17  -0.0 1,871,388 -  Pf1N1B4_1628 0.17  -0.0 1,871,423 +  Pf1N1B4_1628 0.19  -0.0 1,871,423 +  Pf1N1B4_1628 0.19  -0.0 1,871,435 +  Pf1N1B4_1628 0.20  -2.2 1,871,435 +  Pf1N1B4_1628 0.20  -0.0 1,871,435 +  Pf1N1B4_1628 0.20  -2.2 1,871,435 +  Pf1N1B4_1628 0.20  -0.0 1,871,435 +  Pf1N1B4_1628 0.20  -0.0 1,871,436 -  Pf1N1B4_1628 0.20  -0.0 1,871,436 -  Pf1N1B4_1628 0.20  -1.8 1,871,436 -  Pf1N1B4_1628 0.20  -0.0 1,871,436 -  Pf1N1B4_1628 0.20  -0.0 1,871,436 -  Pf1N1B4_1628 0.20  -0.0 1,871,567 +  Pf1N1B4_1628 0.28  -0.0 1,871,568 -  Pf1N1B4_1628 0.28  -0.0 1,871,568 -  Pf1N1B4_1628 0.28  -0.0 1,871,646 -  Pf1N1B4_1628 0.32  -2.4 1,871,646 -  Pf1N1B4_1628 0.32  -0.0 1,871,671 +  Pf1N1B4_1628 0.34  -1.5 1,871,671 +  Pf1N1B4_1628 0.34  -1.7 1,871,672 -  Pf1N1B4_1628 0.34  -0.0 1,871,672 -  Pf1N1B4_1628 0.34  -0.0 1,871,672 -  Pf1N1B4_1628 0.34  -0.0 1,871,678 +  Pf1N1B4_1628 0.34  -1.0 1,871,678 +  Pf1N1B4_1628 0.34  -0.0 1,871,678 +  Pf1N1B4_1628 0.34  -2.4 1,871,679 -  Pf1N1B4_1628 0.34  -0.0 1,871,679 -  Pf1N1B4_1628 0.34  -0.0 1,871,679 -  Pf1N1B4_1628 0.34  -0.0 1,871,679 -  Pf1N1B4_1628 0.34  -0.2 1,871,679 -  Pf1N1B4_1628 0.34  -0.0 1,871,679 -  Pf1N1B4_1628 0.34  -0.0 1,871,679 -  Pf1N1B4_1628 0.34  -0.9 1,871,679 -  Pf1N1B4_1628 0.34  -3.2 1,871,679 -  Pf1N1B4_1628 0.34  -1.7 1,871,679 -  Pf1N1B4_1628 0.34  -0.0 1,871,679 -  Pf1N1B4_1628 0.34  -0.0 1,871,679 -  Pf1N1B4_1628 0.34  -0.0 1,871,699 +  Pf1N1B4_1628 0.36  -0.0 
Or see this region's nucleotide sequence