Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_1525 and Pf1N1B4_1526 are separated by 35 nucleotides Pf1N1B4_1526 and Pf1N1B4_1527 are separated by 369 nucleotides 
        Pf1N1B4_1525 - hypothetical protein, at 1,757,191 to 1,757,448 
        _1525 
         
        
        Pf1N1B4_1526 - hypothetical protein, at 1,757,484 to 1,757,612 
        _1526 
         
        
        Pf1N1B4_1527 - Queuosine Biosynthesis QueC ATPase, at 1,757,982 to 1,758,671 
        _1527 
         Position (kb) 1757 
1758 
1759 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 at 1757.049 kb on - strand at 1757.049 kb on - strand at 1757.056 kb on + strand at 1757.057 kb on - strand at 1757.057 kb on - strand at 1757.057 kb on - strand at 1757.057 kb on - strand at 1757.057 kb on - strand at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.240 kb on + strand, within Pf1N1B4_1525 at 1757.241 kb on - strand, within Pf1N1B4_1525 at 1757.368 kb on + strand, within Pf1N1B4_1525 at 1757.369 kb on - strand, within Pf1N1B4_1525 at 1757.369 kb on - strand, within Pf1N1B4_1525 at 1757.369 kb on - strand, within Pf1N1B4_1525 at 1757.369 kb on - strand, within Pf1N1B4_1525 at 1757.396 kb on + strand, within Pf1N1B4_1525 at 1757.396 kb on + strand, within Pf1N1B4_1525 at 1757.396 kb on + strand, within Pf1N1B4_1525 at 1757.396 kb on + strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.432 kb on + strand at 1757.432 kb on + strand at 1757.432 kb on + strand at 1757.432 kb on + strand at 1757.433 kb on - strand at 1757.610 kb on + strand at 1757.610 kb on + strand at 1757.610 kb on + strand at 1757.636 kb on - strand at 1757.636 kb on - strand at 1757.636 kb on - strand at 1757.636 kb on - strand at 1757.818 kb on + strand at 1757.818 kb on + strand at 1757.820 kb on + strand at 1757.820 kb on + strand at 1757.821 kb on - strand at 1757.921 kb on + strand at 1757.922 kb on - strand at 1757.988 kb on - strand at 1758.312 kb on + strand, within Pf1N1B4_1527 at 1758.313 kb on - strand, within Pf1N1B4_1527 at 1758.440 kb on + strand, within Pf1N1B4_1527 at 1758.507 kb on + strand, within Pf1N1B4_1527 at 1758.507 kb on + strand, within Pf1N1B4_1527 at 1758.524 kb on - strand, within Pf1N1B4_1527 at 1758.536 kb on + strand, within Pf1N1B4_1527 at 1758.557 kb on + strand, within Pf1N1B4_1527 at 1758.557 kb on + strand, within Pf1N1B4_1527 at 1758.613 kb on + strand at 1758.697 kb on + strand at 1758.826 kb on - strand at 1758.840 kb on - strand at 1758.965 kb on + strand at 1759.044 kb on + strand at 1759.067 kb on + strand at 1759.068 kb on - strand at 1759.068 kb on - strand at 1759.138 kb on + strand at 1759.160 kb on - strand at 1759.212 kb on - strand at 1759.292 kb on + strand at 1759.292 kb on + strand at 1759.292 kb on + strand at 1759.292 kb on + strand at 1759.292 kb on + strand at 1759.342 kb on - strand at 1759.346 kb on - strand at 1759.346 kb on - strand at 1759.514 kb on - strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 1,757,049 -  -2.3 1,757,049 -  -0.1 1,757,056 +  -1.9 1,757,057 -  -0.1 1,757,057 -  -0.1 1,757,057 -  -0.1 1,757,057 -  +0.9 1,757,057 -  -0.1 1,757,236 +  Pf1N1B4_1525 0.17  -0.1 1,757,236 +  Pf1N1B4_1525 0.17  -2.6 1,757,236 +  Pf1N1B4_1525 0.17  -0.1 1,757,236 +  Pf1N1B4_1525 0.17  -0.1 1,757,236 +  Pf1N1B4_1525 0.17  -0.1 1,757,236 +  Pf1N1B4_1525 0.17  -0.1 1,757,236 +  Pf1N1B4_1525 0.17  -0.1 1,757,236 +  Pf1N1B4_1525 0.17  -0.1 1,757,237 -  Pf1N1B4_1525 0.18  -1.2 1,757,237 -  Pf1N1B4_1525 0.18  -0.1 1,757,237 -  Pf1N1B4_1525 0.18  -0.1 1,757,237 -  Pf1N1B4_1525 0.18  -0.1 1,757,237 -  Pf1N1B4_1525 0.18  -0.6 1,757,240 +  Pf1N1B4_1525 0.19  -0.1 1,757,241 -  Pf1N1B4_1525 0.19  -0.1 1,757,368 +  Pf1N1B4_1525 0.69  -2.0 1,757,369 -  Pf1N1B4_1525 0.69  -0.1 1,757,369 -  Pf1N1B4_1525 0.69  -0.1 1,757,369 -  Pf1N1B4_1525 0.69  -0.1 1,757,369 -  Pf1N1B4_1525 0.69  -1.6 1,757,396 +  Pf1N1B4_1525 0.79  -0.1 1,757,396 +  Pf1N1B4_1525 0.79  -0.1 1,757,396 +  Pf1N1B4_1525 0.79  -0.1 1,757,396 +  Pf1N1B4_1525 0.79  -0.1 1,757,397 -  Pf1N1B4_1525 0.80  -2.1 1,757,397 -  Pf1N1B4_1525 0.80  -0.1 1,757,397 -  Pf1N1B4_1525 0.80  -0.1 1,757,397 -  Pf1N1B4_1525 0.80  -1.4 1,757,397 -  Pf1N1B4_1525 0.80  -1.3 1,757,432 +  -0.1 1,757,432 +  -0.1 1,757,432 +  -2.5 1,757,432 +  -0.1 1,757,433 -  -3.2 1,757,610 +  -0.1 1,757,610 +  -0.1 1,757,610 +  -0.7 1,757,636 -  -0.1 1,757,636 -  +1.5 1,757,636 -  -1.9 1,757,636 -  -1.1 1,757,818 +  -3.1 1,757,818 +  -0.1 1,757,820 +  -0.1 1,757,820 +  -0.1 1,757,821 -  -2.8 1,757,921 +  -0.1 1,757,922 -  -0.1 1,757,988 -  -0.1 1,758,312 +  Pf1N1B4_1527 0.48  -0.1 1,758,313 -  Pf1N1B4_1527 0.48  -1.2 1,758,440 +  Pf1N1B4_1527 0.66  -0.1 1,758,507 +  Pf1N1B4_1527 0.76  -2.3 1,758,507 +  Pf1N1B4_1527 0.76  -3.4 1,758,524 -  Pf1N1B4_1527 0.79  -0.1 1,758,536 +  Pf1N1B4_1527 0.80  -0.1 1,758,557 +  Pf1N1B4_1527 0.83  -0.1 1,758,557 +  Pf1N1B4_1527 0.83  -0.1 1,758,613 +  -0.1 1,758,697 +  -0.1 1,758,826 -  -0.1 1,758,840 -  -0.1 1,758,965 +  -0.1 1,759,044 +  -0.1 1,759,067 +  -1.5 1,759,068 -  -0.1 1,759,068 -  -0.1 1,759,138 +  -0.1 1,759,160 -  -0.1 1,759,212 -  -2.2 1,759,292 +  -1.2 1,759,292 +  -1.6 1,759,292 +  -0.8 1,759,292 +  +0.4 1,759,292 +  -2.1 1,759,342 -  -0.1 1,759,346 -  -0.1 1,759,346 -  -1.4 1,759,514 -  -1.6 
Or see this region's nucleotide sequence