Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_152

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_151 and Pf1N1B4_152 are separated by 274 nucleotidesPf1N1B4_152 and Pf1N1B4_153 are separated by 213 nucleotides Pf1N1B4_151 - Thioesterase in siderophore biosynthesis gene cluster, at 193,128 to 193,868 _151 Pf1N1B4_152 - ABC transporter, permease protein, at 194,143 to 195,264 _152 Pf1N1B4_153 - Glucoamylase (EC 3.2.1.3), at 195,478 to 197,301 _153 Position (kb) 194 195 196Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 193.190 kb on + strandat 193.813 kb on + strandat 193.813 kb on + strandat 193.875 kb on + strandat 193.894 kb on + strandat 193.895 kb on - strandat 193.925 kb on + strandat 193.959 kb on + strandat 193.960 kb on - strandat 194.091 kb on + strandat 194.091 kb on + strandat 194.091 kb on + strandat 194.092 kb on - strandat 194.112 kb on + strandat 194.552 kb on + strand, within Pf1N1B4_152at 194.718 kb on - strand, within Pf1N1B4_152at 194.718 kb on - strand, within Pf1N1B4_152at 194.725 kb on + strand, within Pf1N1B4_152at 194.725 kb on + strand, within Pf1N1B4_152at 194.856 kb on + strandat 194.857 kb on - strand, within Pf1N1B4_152at 194.902 kb on + strand, within Pf1N1B4_152at 195.024 kb on + strand, within Pf1N1B4_152at 195.025 kb on - strand, within Pf1N1B4_152at 195.445 kb on - strandat 195.449 kb on - strandat 195.871 kb on + strand, within Pf1N1B4_153at 195.916 kb on + strand, within Pf1N1B4_153at 195.917 kb on - strand, within Pf1N1B4_153at 195.969 kb on - strand, within Pf1N1B4_153

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
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193,190 + -0.0
193,813 + -2.9
193,813 + -1.8
193,875 + -0.0
193,894 + -1.6
193,895 - -0.0
193,925 + -1.7
193,959 + -2.7
193,960 - -2.8
194,091 + -0.0
194,091 + -1.5
194,091 + -0.0
194,092 - -0.0
194,112 + -2.0
194,552 + Pf1N1B4_152 0.36 -1.9
194,718 - Pf1N1B4_152 0.51 -1.8
194,718 - Pf1N1B4_152 0.51 -0.0
194,725 + Pf1N1B4_152 0.52 -3.2
194,725 + Pf1N1B4_152 0.52 -0.0
194,856 + -4.0
194,857 - Pf1N1B4_152 0.64 -2.3
194,902 + Pf1N1B4_152 0.68 -1.9
195,024 + Pf1N1B4_152 0.79 -0.0
195,025 - Pf1N1B4_152 0.79 -0.0
195,445 - -2.2
195,449 - -0.0
195,871 + Pf1N1B4_153 0.22 -1.7
195,916 + Pf1N1B4_153 0.24 -0.0
195,917 - Pf1N1B4_153 0.24 -0.0
195,969 - Pf1N1B4_153 0.27 -0.0

Or see this region's nucleotide sequence