Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_134 and Pf1N1B4_135 are separated by 0 nucleotides Pf1N1B4_135 and Pf1N1B4_136 are separated by 7 nucleotides
Pf1N1B4_134 - pyoverdine-specific efflux macA-like protein, at 147,967 to 149,127
_134
Pf1N1B4_135 - Pyoverdine efflux carrier and ATP binding protein, at 149,128 to 151,101
_135
Pf1N1B4_136 - Outer membrane pyoverdine eflux protein, at 151,109 to 152,503
_136
Position (kb) 149
150
151
152 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 148.147 kb on + strand, within Pf1N1B4_134 at 148.147 kb on + strand, within Pf1N1B4_134 at 148.148 kb on - strand, within Pf1N1B4_134 at 148.148 kb on - strand, within Pf1N1B4_134 at 148.148 kb on - strand, within Pf1N1B4_134 at 148.148 kb on - strand, within Pf1N1B4_134 at 148.241 kb on + strand, within Pf1N1B4_134 at 148.241 kb on + strand, within Pf1N1B4_134 at 148.241 kb on + strand, within Pf1N1B4_134 at 148.241 kb on + strand, within Pf1N1B4_134 at 148.242 kb on - strand, within Pf1N1B4_134 at 148.352 kb on - strand, within Pf1N1B4_134 at 148.526 kb on - strand, within Pf1N1B4_134 at 148.526 kb on - strand, within Pf1N1B4_134 at 148.526 kb on - strand, within Pf1N1B4_134 at 148.834 kb on + strand, within Pf1N1B4_134 at 148.899 kb on - strand, within Pf1N1B4_134 at 148.899 kb on - strand, within Pf1N1B4_134 at 149.326 kb on + strand, within Pf1N1B4_135 at 149.470 kb on + strand, within Pf1N1B4_135 at 149.470 kb on + strand, within Pf1N1B4_135 at 149.924 kb on + strand, within Pf1N1B4_135 at 149.924 kb on + strand, within Pf1N1B4_135 at 150.129 kb on + strand, within Pf1N1B4_135 at 150.129 kb on + strand, within Pf1N1B4_135 at 150.256 kb on + strand, within Pf1N1B4_135 at 150.256 kb on + strand, within Pf1N1B4_135 at 150.256 kb on + strand, within Pf1N1B4_135 at 150.257 kb on - strand, within Pf1N1B4_135 at 150.319 kb on + strand, within Pf1N1B4_135 at 150.320 kb on - strand at 150.524 kb on - strand, within Pf1N1B4_135 at 151.108 kb on + strand at 151.109 kb on - strand at 151.448 kb on + strand, within Pf1N1B4_136 at 151.490 kb on + strand, within Pf1N1B4_136 at 151.494 kb on - strand, within Pf1N1B4_136 at 151.640 kb on + strand, within Pf1N1B4_136 at 151.641 kb on - strand, within Pf1N1B4_136 at 151.641 kb on - strand, within Pf1N1B4_136 at 151.641 kb on - strand, within Pf1N1B4_136
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 148,147 + Pf1N1B4_134 0.16 -2.3 148,147 + Pf1N1B4_134 0.16 -1.7 148,148 - Pf1N1B4_134 0.16 -3.0 148,148 - Pf1N1B4_134 0.16 -0.0 148,148 - Pf1N1B4_134 0.16 -0.0 148,148 - Pf1N1B4_134 0.16 -2.7 148,241 + Pf1N1B4_134 0.24 -0.0 148,241 + Pf1N1B4_134 0.24 -0.7 148,241 + Pf1N1B4_134 0.24 -0.0 148,241 + Pf1N1B4_134 0.24 -0.0 148,242 - Pf1N1B4_134 0.24 -0.0 148,352 - Pf1N1B4_134 0.33 -0.0 148,526 - Pf1N1B4_134 0.48 -0.0 148,526 - Pf1N1B4_134 0.48 -0.0 148,526 - Pf1N1B4_134 0.48 -2.2 148,834 + Pf1N1B4_134 0.75 -1.5 148,899 - Pf1N1B4_134 0.80 -1.6 148,899 - Pf1N1B4_134 0.80 -0.0 149,326 + Pf1N1B4_135 0.10 -0.0 149,470 + Pf1N1B4_135 0.17 +1.0 149,470 + Pf1N1B4_135 0.17 -0.0 149,924 + Pf1N1B4_135 0.40 -0.0 149,924 + Pf1N1B4_135 0.40 -3.8 150,129 + Pf1N1B4_135 0.51 -0.0 150,129 + Pf1N1B4_135 0.51 -0.0 150,256 + Pf1N1B4_135 0.57 -2.4 150,256 + Pf1N1B4_135 0.57 -0.0 150,256 + Pf1N1B4_135 0.57 -0.0 150,257 - Pf1N1B4_135 0.57 -0.0 150,319 + Pf1N1B4_135 0.60 -0.0 150,320 - -0.0 150,524 - Pf1N1B4_135 0.71 -2.2 151,108 + -4.1 151,109 - -1.0 151,448 + Pf1N1B4_136 0.24 -1.1 151,490 + Pf1N1B4_136 0.27 -0.0 151,494 - Pf1N1B4_136 0.28 -0.7 151,640 + Pf1N1B4_136 0.38 -0.0 151,641 - Pf1N1B4_136 0.38 -2.2 151,641 - Pf1N1B4_136 0.38 -0.0 151,641 - Pf1N1B4_136 0.38 -1.7
Or see this region's nucleotide sequence