Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_1209 and Pf1N1B4_1210 overlap by 11 nucleotides Pf1N1B4_1210 and Pf1N1B4_1211 are separated by 234 nucleotides Pf1N1B4_1211 and Pf1N1B4_1212 are separated by 418 nucleotides Pf1N1B4_1212 and Pf1N1B4_1213 are separated by 84 nucleotides 
        Pf1N1B4_1209 - FIG00959330: hypothetical protein, at 1,372,393 to 1,372,737 
        _1209 
         
        
        Pf1N1B4_1210 - membrane protein, putative, at 1,372,727 to 1,373,491 
        _1210 
         
        
        Pf1N1B4_1211 - Hydrogen peroxide-inducible genes activator, at 1,373,726 to 1,374,658 
        _1211 
         
        
        Pf1N1B4_1212 - Predicted flavin reductase RutF in novel pyrimidine catabolism pathway, at 1,375,077 to 1,375,379 
        _1212 
         
        
        Pf1N1B4_1213 - Transcriptional regulator, at 1,375,464 to 1,375,739 
        _1213 
         Position (kb) 1373 
1374 
1375 Strain fitness (log2 ratio) -4 
-3 
-2 
-1 
0 
1 at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.760 kb on - strand at 1372.778 kb on + strand at 1372.779 kb on - strand at 1372.998 kb on + strand, within Pf1N1B4_1210 at 1372.998 kb on + strand, within Pf1N1B4_1210 at 1372.999 kb on - strand, within Pf1N1B4_1210 at 1372.999 kb on - strand, within Pf1N1B4_1210 at 1372.999 kb on - strand, within Pf1N1B4_1210 at 1373.133 kb on + strand, within Pf1N1B4_1210 at 1373.133 kb on + strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.135 kb on + strand, within Pf1N1B4_1210 at 1373.136 kb on - strand, within Pf1N1B4_1210 at 1373.441 kb on + strand at 1373.469 kb on + strand at 1373.470 kb on - strand at 1373.485 kb on + strand at 1373.486 kb on - strand at 1373.486 kb on - strand at 1373.498 kb on - strand at 1373.534 kb on + strand at 1373.534 kb on + strand at 1373.534 kb on + strand at 1373.535 kb on - strand at 1373.536 kb on + strand at 1373.536 kb on + strand at 1373.536 kb on + strand at 1373.756 kb on + strand at 1373.757 kb on - strand at 1373.757 kb on - strand at 1374.026 kb on - strand, within Pf1N1B4_1211 at 1374.026 kb on - strand, within Pf1N1B4_1211 at 1374.045 kb on - strand, within Pf1N1B4_1211 at 1374.045 kb on - strand, within Pf1N1B4_1211 at 1374.273 kb on + strand, within Pf1N1B4_1211 at 1374.273 kb on + strand, within Pf1N1B4_1211 at 1374.302 kb on + strand, within Pf1N1B4_1211 at 1374.302 kb on + strand, within Pf1N1B4_1211 at 1374.303 kb on - strand at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.338 kb on + strand, within Pf1N1B4_1211 at 1374.338 kb on + strand, within Pf1N1B4_1211 at 1374.651 kb on + strand at 1374.651 kb on + strand at 1374.651 kb on + strand at 1374.652 kb on - strand at 1374.652 kb on - strand at 1374.652 kb on - strand at 1374.652 kb on - strand at 1374.724 kb on + strand at 1374.724 kb on + strand at 1374.725 kb on - strand at 1374.731 kb on - strand at 1374.737 kb on + strand at 1374.737 kb on + strand at 1374.738 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.763 kb on + strand at 1374.763 kb on + strand at 1374.763 kb on + strand at 1374.763 kb on + strand at 1374.764 kb on - strand at 1374.764 kb on - strand at 1374.764 kb on - strand at 1374.764 kb on - strand at 1374.766 kb on + strand at 1374.767 kb on - strand at 1374.768 kb on + strand at 1374.847 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.872 kb on + strand at 1374.872 kb on + strand at 1374.873 kb on - strand at 1374.873 kb on - strand at 1374.897 kb on - strand at 1375.320 kb on + strand, within Pf1N1B4_1212 at 1375.321 kb on - strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.336 kb on - strand, within Pf1N1B4_1212 at 1375.336 kb on - strand, within Pf1N1B4_1212 at 1375.469 kb on - strand at 1375.494 kb on - strand, within Pf1N1B4_1213 at 1375.494 kb on - strand, within Pf1N1B4_1213 at 1375.495 kb on + strand, within Pf1N1B4_1213 at 1375.496 kb on - strand, within Pf1N1B4_1213 at 1375.496 kb on - strand, within Pf1N1B4_1213 at 1375.502 kb on + strand, within Pf1N1B4_1213 at 1375.502 kb on + strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.514 kb on + strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.547 kb on + strand, within Pf1N1B4_1213  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 1,372,759 +  -0.0 1,372,759 +  -0.0 1,372,759 +  -0.0 1,372,759 +  -0.0 1,372,759 +  -0.0 1,372,759 +  -1.9 1,372,760 -  -1.6 1,372,778 +  -0.0 1,372,779 -  -0.0 1,372,998 +  Pf1N1B4_1210 0.35  -2.0 1,372,998 +  Pf1N1B4_1210 0.35  -0.0 1,372,999 -  Pf1N1B4_1210 0.36  -0.0 1,372,999 -  Pf1N1B4_1210 0.36  -2.2 1,372,999 -  Pf1N1B4_1210 0.36  -1.8 1,373,133 +  Pf1N1B4_1210 0.53  -2.1 1,373,133 +  Pf1N1B4_1210 0.53  +1.0 1,373,134 -  Pf1N1B4_1210 0.53  -0.0 1,373,134 -  Pf1N1B4_1210 0.53  -0.0 1,373,134 -  Pf1N1B4_1210 0.53  -0.0 1,373,134 -  Pf1N1B4_1210 0.53  -0.0 1,373,134 -  Pf1N1B4_1210 0.53  -0.0 1,373,134 -  Pf1N1B4_1210 0.53  -0.0 1,373,134 -  +0.5 1,373,134 -  Pf1N1B4_1210 0.53  -0.0 1,373,134 -  Pf1N1B4_1210 0.53  -2.1 1,373,135 +  Pf1N1B4_1210 0.53  -1.1 1,373,136 -  Pf1N1B4_1210 0.53  -1.6 1,373,441 +  -0.0 1,373,469 +  -1.4 1,373,470 -  -0.0 1,373,485 +  -1.7 1,373,486 -  -0.0 1,373,486 -  -0.0 1,373,498 -  -3.7 1,373,534 +  -0.0 1,373,534 +  -0.0 1,373,534 +  -0.0 1,373,535 -  -0.0 1,373,536 +  -0.0 1,373,536 +  -1.3 1,373,536 +  -1.8 1,373,756 +  -3.5 1,373,757 -  -0.0 1,373,757 -  -1.3 1,374,026 -  Pf1N1B4_1211 0.32  -1.0 1,374,026 -  Pf1N1B4_1211 0.32  -2.2 1,374,045 -  Pf1N1B4_1211 0.34  -3.5 1,374,045 -  Pf1N1B4_1211 0.34  -0.0 1,374,273 +  Pf1N1B4_1211 0.59  -0.0 1,374,273 +  Pf1N1B4_1211 0.59  -0.0 1,374,302 +  Pf1N1B4_1211 0.62  -1.8 1,374,302 +  Pf1N1B4_1211 0.62  -0.0 1,374,303 -  -0.0 1,374,303 -  Pf1N1B4_1211 0.62  +1.0 1,374,303 -  Pf1N1B4_1211 0.62  -0.0 1,374,303 -  Pf1N1B4_1211 0.62  -0.0 1,374,303 -  Pf1N1B4_1211 0.62  -2.8 1,374,303 -  Pf1N1B4_1211 0.62  -3.3 1,374,303 -  Pf1N1B4_1211 0.62  -0.0 1,374,303 -  Pf1N1B4_1211 0.62  -0.0 1,374,303 -  -0.0 1,374,303 -  Pf1N1B4_1211 0.62  -0.0 1,374,338 +  Pf1N1B4_1211 0.66  -0.0 1,374,338 +  Pf1N1B4_1211 0.66  -0.0 1,374,651 +  -0.0 1,374,651 +  -0.0 1,374,651 +  -0.0 1,374,652 -  -1.5 1,374,652 -  -0.0 1,374,652 -  -0.0 1,374,652 -  -0.0 1,374,724 +  -1.1 1,374,724 +  -0.0 1,374,725 -  -0.0 1,374,731 -  +1.0 1,374,737 +  -0.0 1,374,737 +  -0.0 1,374,738 -  -0.0 1,374,760 -  -1.5 1,374,760 -  -0.0 1,374,760 -  -0.0 1,374,760 -  -0.0 1,374,760 -  -0.0 1,374,763 +  -0.0 1,374,763 +  -0.0 1,374,763 +  -1.3 1,374,763 +  -0.0 1,374,764 -  -2.3 1,374,764 -  -0.0 1,374,764 -  -2.4 1,374,764 -  -2.0 1,374,766 +  -0.0 1,374,767 -  -0.0 1,374,768 +  -0.0 1,374,847 +  -0.0 1,374,852 +  -0.6 1,374,852 +  -0.0 1,374,852 +  -0.7 1,374,852 +  -0.0 1,374,852 +  -0.8 1,374,853 -  -1.9 1,374,853 -  -0.0 1,374,853 -  -1.7 1,374,853 -  -0.0 1,374,853 -  -0.0 1,374,853 -  -2.3 1,374,853 -  -1.7 1,374,853 -  -0.0 1,374,853 -  -0.3 1,374,872 +  -0.0 1,374,872 +  -1.4 1,374,873 -  -1.8 1,374,873 -  -0.0 1,374,897 -  -1.9 1,375,320 +  Pf1N1B4_1212 0.80  -0.6 1,375,321 -  Pf1N1B4_1212 0.81  -0.0 1,375,335 +  Pf1N1B4_1212 0.85  -1.3 1,375,335 +  Pf1N1B4_1212 0.85  -1.9 1,375,335 +  Pf1N1B4_1212 0.85  -0.0 1,375,335 +  Pf1N1B4_1212 0.85  -0.0 1,375,335 +  Pf1N1B4_1212 0.85  -0.0 1,375,335 +  Pf1N1B4_1212 0.85  +0.5 1,375,336 -  Pf1N1B4_1212 0.85  -0.0 1,375,336 -  Pf1N1B4_1212 0.85  -0.0 1,375,469 -  -2.3 1,375,494 -  Pf1N1B4_1213 0.11  -0.0 1,375,494 -  Pf1N1B4_1213 0.11  -0.0 1,375,495 +  Pf1N1B4_1213 0.11  -0.0 1,375,496 -  Pf1N1B4_1213 0.12  -0.0 1,375,496 -  Pf1N1B4_1213 0.12  -1.7 1,375,502 +  Pf1N1B4_1213 0.14  -1.8 1,375,502 +  Pf1N1B4_1213 0.14  -0.0 1,375,503 -  Pf1N1B4_1213 0.14  -0.0 1,375,503 -  Pf1N1B4_1213 0.14  -0.0 1,375,503 -  Pf1N1B4_1213 0.14  -0.0 1,375,503 -  Pf1N1B4_1213 0.14  -0.0 1,375,503 -  Pf1N1B4_1213 0.14  -4.5 1,375,514 +  Pf1N1B4_1213 0.18  -1.0 1,375,515 -  Pf1N1B4_1213 0.18  -0.0 1,375,515 -  Pf1N1B4_1213 0.18  -0.0 1,375,515 -  Pf1N1B4_1213 0.18  -0.0 1,375,515 -  Pf1N1B4_1213 0.18  -0.1 1,375,547 +  Pf1N1B4_1213 0.30  -2.1 
Or see this region's nucleotide sequence