Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1010 and Pf1N1B4_1011 are separated by 143 nucleotides Pf1N1B4_1011 and Pf1N1B4_1012 are separated by 216 nucleotides
Pf1N1B4_1010 - Iron(III) dicitrate transport protein FecA @ Iron siderophore receptor protein, at 1,144,523 to 1,146,856
_1010
Pf1N1B4_1011 - ribosomal subunit interface protein, putative, at 1,147,000 to 1,147,407
_1011
Pf1N1B4_1012 - Glycine cleavage system transcriptional activator, at 1,147,624 to 1,148,571
_1012
Position (kb) 1146
1147
1148 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1146.080 kb on + strand, within Pf1N1B4_1010 at 1146.080 kb on + strand, within Pf1N1B4_1010 at 1146.080 kb on + strand, within Pf1N1B4_1010 at 1146.080 kb on + strand, within Pf1N1B4_1010 at 1146.080 kb on + strand, within Pf1N1B4_1010 at 1146.080 kb on + strand, within Pf1N1B4_1010 at 1146.081 kb on - strand, within Pf1N1B4_1010 at 1146.125 kb on + strand, within Pf1N1B4_1010 at 1146.125 kb on + strand, within Pf1N1B4_1010 at 1146.125 kb on + strand, within Pf1N1B4_1010 at 1146.126 kb on - strand, within Pf1N1B4_1010 at 1146.188 kb on + strand, within Pf1N1B4_1010 at 1146.189 kb on - strand, within Pf1N1B4_1010 at 1146.326 kb on + strand, within Pf1N1B4_1010 at 1146.326 kb on + strand, within Pf1N1B4_1010 at 1146.404 kb on + strand, within Pf1N1B4_1010 at 1146.405 kb on - strand, within Pf1N1B4_1010 at 1146.450 kb on - strand, within Pf1N1B4_1010 at 1146.458 kb on + strand, within Pf1N1B4_1010 at 1146.458 kb on + strand, within Pf1N1B4_1010 at 1146.458 kb on + strand, within Pf1N1B4_1010 at 1146.458 kb on + strand, within Pf1N1B4_1010 at 1146.458 kb on + strand, within Pf1N1B4_1010 at 1146.458 kb on + strand, within Pf1N1B4_1010 at 1146.458 kb on + strand, within Pf1N1B4_1010 at 1146.459 kb on - strand, within Pf1N1B4_1010 at 1146.459 kb on - strand at 1146.459 kb on - strand, within Pf1N1B4_1010 at 1146.459 kb on - strand, within Pf1N1B4_1010 at 1146.464 kb on + strand at 1146.464 kb on + strand, within Pf1N1B4_1010 at 1146.465 kb on - strand, within Pf1N1B4_1010 at 1146.465 kb on - strand, within Pf1N1B4_1010 at 1146.465 kb on - strand, within Pf1N1B4_1010 at 1146.465 kb on - strand, within Pf1N1B4_1010 at 1146.465 kb on - strand, within Pf1N1B4_1010 at 1146.465 kb on - strand, within Pf1N1B4_1010 at 1146.593 kb on + strand, within Pf1N1B4_1010 at 1146.594 kb on - strand, within Pf1N1B4_1010 at 1146.594 kb on - strand, within Pf1N1B4_1010 at 1146.594 kb on - strand, within Pf1N1B4_1010 at 1146.671 kb on + strand at 1146.671 kb on + strand at 1146.671 kb on + strand at 1146.671 kb on + strand at 1146.671 kb on + strand at 1146.672 kb on - strand at 1146.672 kb on - strand at 1146.679 kb on - strand at 1146.698 kb on + strand at 1146.698 kb on + strand at 1146.698 kb on + strand at 1146.699 kb on - strand at 1146.699 kb on - strand at 1146.767 kb on + strand at 1146.767 kb on + strand at 1146.767 kb on + strand at 1146.767 kb on + strand at 1146.767 kb on + strand at 1146.767 kb on + strand at 1146.768 kb on - strand at 1146.768 kb on - strand at 1146.806 kb on + strand at 1146.806 kb on + strand at 1146.806 kb on + strand at 1146.806 kb on + strand at 1146.806 kb on + strand at 1146.806 kb on + strand at 1146.806 kb on + strand at 1146.806 kb on + strand at 1146.807 kb on - strand at 1146.807 kb on - strand at 1146.807 kb on - strand at 1146.807 kb on - strand at 1146.807 kb on - strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.831 kb on - strand at 1146.831 kb on - strand at 1146.831 kb on - strand at 1146.831 kb on - strand at 1146.831 kb on - strand at 1146.831 kb on - strand at 1146.831 kb on - strand at 1146.831 kb on - strand at 1146.831 kb on - strand at 1146.831 kb on - strand at 1146.923 kb on + strand at 1146.923 kb on + strand at 1146.924 kb on - strand at 1146.943 kb on + strand at 1146.944 kb on - strand at 1146.984 kb on - strand at 1146.984 kb on - strand at 1146.984 kb on - strand at 1146.989 kb on + strand at 1146.989 kb on + strand at 1146.990 kb on - strand at 1146.990 kb on - strand at 1147.186 kb on - strand, within Pf1N1B4_1011 at 1147.314 kb on - strand, within Pf1N1B4_1011 at 1147.314 kb on - strand, within Pf1N1B4_1011 at 1147.338 kb on + strand, within Pf1N1B4_1011 at 1147.338 kb on + strand, within Pf1N1B4_1011 at 1147.341 kb on + strand, within Pf1N1B4_1011 at 1147.341 kb on + strand, within Pf1N1B4_1011 at 1147.341 kb on + strand, within Pf1N1B4_1011 at 1147.341 kb on + strand, within Pf1N1B4_1011 at 1147.341 kb on + strand, within Pf1N1B4_1011 at 1147.341 kb on + strand, within Pf1N1B4_1011 at 1147.342 kb on - strand, within Pf1N1B4_1011 at 1147.342 kb on - strand, within Pf1N1B4_1011 at 1147.342 kb on - strand at 1147.342 kb on - strand, within Pf1N1B4_1011 at 1147.342 kb on - strand, within Pf1N1B4_1011 at 1147.342 kb on - strand, within Pf1N1B4_1011 at 1147.346 kb on - strand, within Pf1N1B4_1011 at 1147.377 kb on + strand at 1147.407 kb on + strand at 1147.408 kb on - strand at 1147.408 kb on - strand at 1147.408 kb on - strand at 1147.408 kb on - strand at 1147.408 kb on - strand at 1147.408 kb on - strand at 1147.408 kb on - strand at 1147.408 kb on - strand at 1147.420 kb on - strand at 1147.420 kb on - strand at 1147.420 kb on - strand at 1147.429 kb on - strand at 1147.429 kb on - strand at 1147.429 kb on - strand at 1147.536 kb on - strand at 1147.578 kb on + strand at 1147.656 kb on + strand at 1147.657 kb on - strand at 1147.657 kb on - strand at 1147.664 kb on - strand at 1147.664 kb on - strand at 1147.664 kb on - strand at 1147.866 kb on + strand, within Pf1N1B4_1012 at 1147.890 kb on + strand, within Pf1N1B4_1012 at 1147.891 kb on - strand, within Pf1N1B4_1012 at 1147.937 kb on + strand, within Pf1N1B4_1012 at 1147.937 kb on + strand, within Pf1N1B4_1012 at 1147.937 kb on + strand, within Pf1N1B4_1012 at 1147.938 kb on - strand, within Pf1N1B4_1012 at 1147.938 kb on - strand, within Pf1N1B4_1012 at 1147.938 kb on - strand, within Pf1N1B4_1012 at 1147.956 kb on - strand, within Pf1N1B4_1012 at 1147.961 kb on + strand, within Pf1N1B4_1012 at 1147.962 kb on - strand, within Pf1N1B4_1012 at 1148.071 kb on + strand at 1148.072 kb on - strand, within Pf1N1B4_1012 at 1148.072 kb on - strand, within Pf1N1B4_1012 at 1148.072 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 1,146,080 + Pf1N1B4_1010 0.67 +0.0 1,146,080 + Pf1N1B4_1010 0.67 -1.7 1,146,080 + Pf1N1B4_1010 0.67 +0.0 1,146,080 + Pf1N1B4_1010 0.67 +0.0 1,146,080 + Pf1N1B4_1010 0.67 -1.0 1,146,080 + Pf1N1B4_1010 0.67 -2.2 1,146,081 - Pf1N1B4_1010 0.67 +0.0 1,146,125 + Pf1N1B4_1010 0.69 +0.0 1,146,125 + Pf1N1B4_1010 0.69 +0.0 1,146,125 + Pf1N1B4_1010 0.69 +0.0 1,146,126 - Pf1N1B4_1010 0.69 -3.4 1,146,188 + Pf1N1B4_1010 0.71 -2.5 1,146,189 - Pf1N1B4_1010 0.71 -2.5 1,146,326 + Pf1N1B4_1010 0.77 +0.0 1,146,326 + Pf1N1B4_1010 0.77 +0.0 1,146,404 + Pf1N1B4_1010 0.81 -1.9 1,146,405 - Pf1N1B4_1010 0.81 +0.0 1,146,450 - Pf1N1B4_1010 0.83 -1.6 1,146,458 + Pf1N1B4_1010 0.83 +0.0 1,146,458 + Pf1N1B4_1010 0.83 +0.0 1,146,458 + Pf1N1B4_1010 0.83 -1.3 1,146,458 + Pf1N1B4_1010 0.83 -2.9 1,146,458 + Pf1N1B4_1010 0.83 -1.8 1,146,458 + Pf1N1B4_1010 0.83 +0.0 1,146,458 + Pf1N1B4_1010 0.83 +0.0 1,146,459 - Pf1N1B4_1010 0.83 +0.0 1,146,459 - +0.0 1,146,459 - Pf1N1B4_1010 0.83 +0.0 1,146,459 - Pf1N1B4_1010 0.83 -1.7 1,146,464 + +0.0 1,146,464 + Pf1N1B4_1010 0.83 +0.0 1,146,465 - Pf1N1B4_1010 0.83 +0.0 1,146,465 - Pf1N1B4_1010 0.83 -2.0 1,146,465 - Pf1N1B4_1010 0.83 -0.9 1,146,465 - Pf1N1B4_1010 0.83 -1.6 1,146,465 - Pf1N1B4_1010 0.83 -1.6 1,146,465 - Pf1N1B4_1010 0.83 -1.6 1,146,593 + Pf1N1B4_1010 0.89 -2.9 1,146,594 - Pf1N1B4_1010 0.89 -0.9 1,146,594 - Pf1N1B4_1010 0.89 -2.0 1,146,594 - Pf1N1B4_1010 0.89 -2.2 1,146,671 + +0.0 1,146,671 + +0.0 1,146,671 + -2.0 1,146,671 + +0.0 1,146,671 + +0.0 1,146,672 - +0.0 1,146,672 - +0.0 1,146,679 - -0.6 1,146,698 + -2.2 1,146,698 + +0.0 1,146,698 + +0.0 1,146,699 - +0.0 1,146,699 - +0.0 1,146,767 + +0.0 1,146,767 + -1.4 1,146,767 + -1.4 1,146,767 + -0.7 1,146,767 + -2.4 1,146,767 + +0.0 1,146,768 - -4.1 1,146,768 - +0.0 1,146,806 + +0.0 1,146,806 + -0.6 1,146,806 + +0.0 1,146,806 + +0.0 1,146,806 + +0.0 1,146,806 + +0.0 1,146,806 + -1.9 1,146,806 + +0.0 1,146,807 - +0.0 1,146,807 - +0.0 1,146,807 - +0.0 1,146,807 - +0.0 1,146,807 - -1.8 1,146,830 + -0.9 1,146,830 + +0.0 1,146,830 + +0.0 1,146,830 + -1.9 1,146,830 + +0.0 1,146,830 + -1.6 1,146,830 + -0.9 1,146,830 + +0.0 1,146,830 + -1.5 1,146,830 + -1.1 1,146,830 + -1.3 1,146,830 + -2.9 1,146,830 + +0.0 1,146,830 + +0.0 1,146,830 + -0.2 1,146,831 - +0.0 1,146,831 - -0.7 1,146,831 - +0.0 1,146,831 - +0.0 1,146,831 - -2.2 1,146,831 - +0.0 1,146,831 - +0.0 1,146,831 - +0.0 1,146,831 - -1.8 1,146,831 - -2.2 1,146,923 + +0.0 1,146,923 + -2.1 1,146,924 - +0.0 1,146,943 + -2.2 1,146,944 - +0.0 1,146,984 - +0.0 1,146,984 - -1.6 1,146,984 - +0.0 1,146,989 + +0.0 1,146,989 + +0.0 1,146,990 - -2.1 1,146,990 - +0.0 1,147,186 - Pf1N1B4_1011 0.46 +0.0 1,147,314 - Pf1N1B4_1011 0.77 +0.0 1,147,314 - Pf1N1B4_1011 0.77 -2.3 1,147,338 + Pf1N1B4_1011 0.83 +0.0 1,147,338 + Pf1N1B4_1011 0.83 -2.3 1,147,341 + Pf1N1B4_1011 0.84 +0.0 1,147,341 + Pf1N1B4_1011 0.84 +0.0 1,147,341 + Pf1N1B4_1011 0.84 +0.0 1,147,341 + Pf1N1B4_1011 0.84 +0.0 1,147,341 + Pf1N1B4_1011 0.84 -2.8 1,147,341 + Pf1N1B4_1011 0.84 +0.0 1,147,342 - Pf1N1B4_1011 0.84 -2.4 1,147,342 - Pf1N1B4_1011 0.84 +0.0 1,147,342 - +0.0 1,147,342 - Pf1N1B4_1011 0.84 +0.0 1,147,342 - Pf1N1B4_1011 0.84 +0.0 1,147,342 - Pf1N1B4_1011 0.84 +0.0 1,147,346 - Pf1N1B4_1011 0.85 -2.5 1,147,377 + +0.0 1,147,407 + -2.2 1,147,408 - +0.0 1,147,408 - +0.0 1,147,408 - +0.0 1,147,408 - -1.9 1,147,408 - -1.5 1,147,408 - +0.0 1,147,408 - -2.0 1,147,408 - +0.0 1,147,420 - -1.9 1,147,420 - +0.0 1,147,420 - +0.0 1,147,429 - -1.4 1,147,429 - -2.3 1,147,429 - -2.5 1,147,536 - +0.0 1,147,578 + +0.0 1,147,656 + -1.3 1,147,657 - +0.0 1,147,657 - +0.0 1,147,664 - +0.0 1,147,664 - -1.5 1,147,664 - +0.0 1,147,866 + Pf1N1B4_1012 0.26 +0.0 1,147,890 + Pf1N1B4_1012 0.28 +0.0 1,147,891 - Pf1N1B4_1012 0.28 +0.0 1,147,937 + Pf1N1B4_1012 0.33 -1.0 1,147,937 + Pf1N1B4_1012 0.33 +0.0 1,147,937 + Pf1N1B4_1012 0.33 -1.9 1,147,938 - Pf1N1B4_1012 0.33 -1.9 1,147,938 - Pf1N1B4_1012 0.33 +0.0 1,147,938 - Pf1N1B4_1012 0.33 +0.0 1,147,956 - Pf1N1B4_1012 0.35 +0.0 1,147,961 + Pf1N1B4_1012 0.36 -1.3 1,147,962 - Pf1N1B4_1012 0.36 -1.7 1,148,071 + +0.0 1,148,072 - Pf1N1B4_1012 0.47 +0.0 1,148,072 - Pf1N1B4_1012 0.47 -2.2 1,148,072 - +0.0
Or see this region's nucleotide sequence