Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c35150 and PGA1_c35160 overlap by 4 nucleotides PGA1_c35160 and PGA1_c35170 overlap by 8 nucleotides PGA1_c35170 and PGA1_c35180 are separated by 75 nucleotides PGA1_c35180 and PGA1_c35190 are separated by 78 nucleotides
PGA1_c35150: PGA1_c35150 - shikimate dehydrogenase AroE, at 3,687,966 to 3,688,799
_c35150
PGA1_c35160: PGA1_c35160 - dephospho-CoA kinase CoaE, at 3,688,796 to 3,689,389
_c35160
PGA1_c35170: PGA1_c35170 - DNA polymerase III subunit epsilon, at 3,689,382 to 3,690,119
_c35170
PGA1_c35180: PGA1_c35180 - protein-export protein SecB, at 3,690,195 to 3,690,689
_c35180
PGA1_c35190: PGA1_c35190 - putative protein FxsA, at 3,690,768 to 3,691,274
_c35190
Position (kb)
3689
3690
3691 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3688.793 kb on + strand at 3688.793 kb on + strand at 3688.793 kb on + strand at 3689.458 kb on + strand, within PGA1_c35170 at 3689.505 kb on + strand, within PGA1_c35170 at 3689.568 kb on - strand, within PGA1_c35170 at 3689.724 kb on - strand, within PGA1_c35170 at 3689.989 kb on + strand, within PGA1_c35170 at 3690.007 kb on + strand, within PGA1_c35170 at 3690.093 kb on + strand at 3690.096 kb on + strand at 3690.096 kb on + strand at 3690.096 kb on + strand at 3690.098 kb on + strand at 3690.104 kb on - strand at 3690.161 kb on - strand at 3690.186 kb on + strand at 3690.186 kb on + strand at 3690.186 kb on + strand at 3690.194 kb on - strand at 3690.194 kb on - strand at 3690.194 kb on - strand at 3690.204 kb on + strand at 3690.225 kb on - strand at 3690.225 kb on - strand at 3690.225 kb on - strand at 3690.225 kb on - strand at 3690.766 kb on + strand at 3690.771 kb on + strand at 3690.779 kb on + strand at 3690.860 kb on - strand, within PGA1_c35190 at 3690.860 kb on - strand, within PGA1_c35190 at 3690.880 kb on + strand, within PGA1_c35190 at 3690.923 kb on + strand, within PGA1_c35190 at 3690.987 kb on + strand, within PGA1_c35190 at 3690.987 kb on + strand, within PGA1_c35190 at 3690.995 kb on - strand, within PGA1_c35190 at 3691.029 kb on - strand, within PGA1_c35190 at 3691.036 kb on - strand, within PGA1_c35190
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,688,793 + -2.0 3,688,793 + -1.5 3,688,793 + -0.3 3,689,458 + PGA1_c35170 0.10 +0.6 3,689,505 + PGA1_c35170 0.17 -0.5 3,689,568 - PGA1_c35170 0.25 +1.5 3,689,724 - PGA1_c35170 0.46 -1.9 3,689,989 + PGA1_c35170 0.82 -0.4 3,690,007 + PGA1_c35170 0.85 -0.4 3,690,093 + +0.4 3,690,096 + +0.2 3,690,096 + -0.9 3,690,096 + -1.1 3,690,098 + -0.6 3,690,104 - +2.0 3,690,161 - +0.2 3,690,186 + +0.3 3,690,186 + +0.6 3,690,186 + -0.1 3,690,194 - +1.0 3,690,194 - -0.7 3,690,194 - -1.6 3,690,204 + +0.2 3,690,225 - +0.5 3,690,225 - -1.1 3,690,225 - +0.4 3,690,225 - +0.6 3,690,766 + +0.2 3,690,771 + +1.3 3,690,779 + +0.6 3,690,860 - PGA1_c35190 0.18 -0.0 3,690,860 - PGA1_c35190 0.18 -1.8 3,690,880 + PGA1_c35190 0.22 -0.2 3,690,923 + PGA1_c35190 0.31 -1.2 3,690,987 + PGA1_c35190 0.43 -0.2 3,690,987 + PGA1_c35190 0.43 -1.4 3,690,995 - PGA1_c35190 0.45 -0.2 3,691,029 - PGA1_c35190 0.51 +0.6 3,691,036 - PGA1_c35190 0.53 -0.2
Or see this region's nucleotide sequence