Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22195 and BWI76_RS22200 are separated by 8 nucleotides BWI76_RS22200 and BWI76_RS22205 overlap by 4 nucleotides BWI76_RS22205 and BWI76_RS22210 are separated by 236 nucleotides
BWI76_RS22195: BWI76_RS22195 - metal ABC transporter substrate-binding protein, at 4,628,124 to 4,629,002
_RS22195
BWI76_RS22200: BWI76_RS22200 - iron ABC transporter, at 4,629,011 to 4,629,874
_RS22200
BWI76_RS22205: BWI76_RS22205 - cation ABC transporter ATP-binding protein, at 4,629,871 to 4,630,548
_RS22205
BWI76_RS22210: BWI76_RS22210 - murein transglycosylase B, at 4,630,785 to 4,631,861
_RS22210
Position (kb) 4629
4630
4631 Strain fitness (log2 ratio) -1
0
1
2 at 4628.901 kb on + strand, within BWI76_RS22195 at 4628.902 kb on - strand, within BWI76_RS22195 at 4629.105 kb on - strand, within BWI76_RS22200 at 4629.105 kb on - strand, within BWI76_RS22200 at 4629.148 kb on - strand, within BWI76_RS22200 at 4629.389 kb on - strand, within BWI76_RS22200 at 4629.396 kb on + strand, within BWI76_RS22200 at 4629.429 kb on - strand, within BWI76_RS22200 at 4629.429 kb on - strand, within BWI76_RS22200 at 4629.849 kb on + strand at 4629.927 kb on - strand at 4630.123 kb on - strand, within BWI76_RS22205 at 4630.233 kb on + strand, within BWI76_RS22205 at 4630.241 kb on - strand, within BWI76_RS22205 at 4630.497 kb on + strand at 4630.645 kb on + strand at 4631.308 kb on - strand, within BWI76_RS22210 at 4631.423 kb on + strand, within BWI76_RS22210 at 4631.433 kb on - strand, within BWI76_RS22210 at 4631.443 kb on + strand, within BWI76_RS22210 at 4631.453 kb on - strand, within BWI76_RS22210 at 4631.473 kb on - strand, within BWI76_RS22210
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 4,628,901 + BWI76_RS22195 0.88 -0.2 4,628,902 - BWI76_RS22195 0.89 +0.8 4,629,105 - BWI76_RS22200 0.11 -0.7 4,629,105 - BWI76_RS22200 0.11 -0.1 4,629,148 - BWI76_RS22200 0.16 +0.2 4,629,389 - BWI76_RS22200 0.44 +0.7 4,629,396 + BWI76_RS22200 0.45 +0.1 4,629,429 - BWI76_RS22200 0.48 -0.1 4,629,429 - BWI76_RS22200 0.48 +2.6 4,629,849 + -0.8 4,629,927 - -0.2 4,630,123 - BWI76_RS22205 0.37 -0.1 4,630,233 + BWI76_RS22205 0.53 -0.0 4,630,241 - BWI76_RS22205 0.55 -0.3 4,630,497 + -0.2 4,630,645 + +0.1 4,631,308 - BWI76_RS22210 0.49 +0.4 4,631,423 + BWI76_RS22210 0.59 +0.3 4,631,433 - BWI76_RS22210 0.60 +0.2 4,631,443 + BWI76_RS22210 0.61 -0.3 4,631,453 - BWI76_RS22210 0.62 +0.3 4,631,473 - BWI76_RS22210 0.64 +0.6
Or see this region's nucleotide sequence