Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19695 and BWI76_RS19700 are separated by 95 nucleotides BWI76_RS19700 and BWI76_RS19705 are separated by 1 nucleotides BWI76_RS19705 and BWI76_RS19710 are separated by 32 nucleotides
BWI76_RS19695: BWI76_RS19695 - membrane protein, at 4,116,302 to 4,117,351
_RS19695
BWI76_RS19700: BWI76_RS19700 - deoxyribonuclease IV, at 4,117,447 to 4,118,304
_RS19700
BWI76_RS19705: BWI76_RS19705 - kinase, at 4,118,306 to 4,119,394
_RS19705
BWI76_RS19710: BWI76_RS19710 - NupC/NupG family nucleoside CNT transporter, at 4,119,427 to 4,120,677
_RS19710
Position (kb)
4118
4119
4120 Strain fitness (log2 ratio)
-1
0
1 at 4118.021 kb on + strand, within BWI76_RS19700 at 4118.029 kb on - strand, within BWI76_RS19700 at 4118.087 kb on + strand, within BWI76_RS19700 at 4118.324 kb on - strand at 4118.511 kb on - strand, within BWI76_RS19705 at 4118.776 kb on + strand, within BWI76_RS19705 at 4118.876 kb on + strand, within BWI76_RS19705 at 4118.918 kb on + strand, within BWI76_RS19705 at 4119.258 kb on - strand, within BWI76_RS19705 at 4119.258 kb on - strand, within BWI76_RS19705 at 4119.470 kb on + strand at 4119.474 kb on - strand at 4119.744 kb on - strand, within BWI76_RS19710 at 4119.813 kb on + strand, within BWI76_RS19710 at 4120.020 kb on + strand, within BWI76_RS19710
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 4,118,021 + BWI76_RS19700 0.67 +0.3 4,118,029 - BWI76_RS19700 0.68 +0.6 4,118,087 + BWI76_RS19700 0.75 -0.1 4,118,324 - +0.1 4,118,511 - BWI76_RS19705 0.19 -0.3 4,118,776 + BWI76_RS19705 0.43 -0.6 4,118,876 + BWI76_RS19705 0.52 -1.7 4,118,918 + BWI76_RS19705 0.56 -0.7 4,119,258 - BWI76_RS19705 0.87 -0.7 4,119,258 - BWI76_RS19705 0.87 -0.7 4,119,470 + +0.2 4,119,474 - -0.6 4,119,744 - BWI76_RS19710 0.25 +0.3 4,119,813 + BWI76_RS19710 0.31 -0.2 4,120,020 + BWI76_RS19710 0.47 +0.3
Or see this region's nucleotide sequence