Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06220 and BWI76_RS06225 overlap by 4 nucleotides BWI76_RS06225 and BWI76_RS06230 are separated by 294 nucleotides
BWI76_RS06220: BWI76_RS06220 - exonuclease subunit SbcC, at 1,303,115 to 1,306,249
_RS06220
BWI76_RS06225: BWI76_RS06225 - exonuclease sbcCD subunit D, at 1,306,246 to 1,307,448
_RS06225
BWI76_RS06230: BWI76_RS06230 - DNA-binding response regulator, at 1,307,743 to 1,308,432
_RS06230
Position (kb)
1306
1307
1308 Strain fitness (log2 ratio)
-1
0
1
2 at 1305.804 kb on - strand, within BWI76_RS06220 at 1306.072 kb on - strand at 1306.072 kb on - strand at 1306.174 kb on + strand at 1306.296 kb on + strand at 1306.427 kb on - strand, within BWI76_RS06225 at 1306.854 kb on - strand, within BWI76_RS06225 at 1306.946 kb on - strand, within BWI76_RS06225 at 1307.040 kb on - strand, within BWI76_RS06225 at 1307.278 kb on + strand, within BWI76_RS06225 at 1307.278 kb on + strand, within BWI76_RS06225 at 1307.431 kb on + strand at 1307.432 kb on - strand at 1307.514 kb on - strand at 1307.514 kb on - strand at 1307.530 kb on + strand at 1307.540 kb on - strand at 1307.567 kb on + strand at 1307.575 kb on - strand at 1307.707 kb on + strand at 1307.707 kb on + strand at 1307.707 kb on + strand at 1307.855 kb on + strand, within BWI76_RS06230 at 1307.865 kb on + strand, within BWI76_RS06230 at 1307.933 kb on + strand, within BWI76_RS06230 at 1308.435 kb on + strand at 1308.444 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 1,305,804 - BWI76_RS06220 0.86 +0.3 1,306,072 - -0.0 1,306,072 - -0.7 1,306,174 + +0.1 1,306,296 + +0.2 1,306,427 - BWI76_RS06225 0.15 -1.2 1,306,854 - BWI76_RS06225 0.51 -1.3 1,306,946 - BWI76_RS06225 0.58 -0.4 1,307,040 - BWI76_RS06225 0.66 -1.0 1,307,278 + BWI76_RS06225 0.86 +0.3 1,307,278 + BWI76_RS06225 0.86 +0.1 1,307,431 + +0.4 1,307,432 - -0.4 1,307,514 - +0.2 1,307,514 - +1.0 1,307,530 + -0.1 1,307,540 - +0.9 1,307,567 + -0.9 1,307,575 - -0.1 1,307,707 + +0.1 1,307,707 + -0.4 1,307,707 + -1.7 1,307,855 + BWI76_RS06230 0.16 +1.9 1,307,865 + BWI76_RS06230 0.18 +0.2 1,307,933 + BWI76_RS06230 0.28 -0.5 1,308,435 + -0.4 1,308,444 + -0.6
Or see this region's nucleotide sequence