Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05995 and BWI76_RS06000 are separated by 366 nucleotides BWI76_RS06000 and BWI76_RS06005 overlap by 4 nucleotides
BWI76_RS05995: BWI76_RS05995 - branched-chain amino acid ABC transporter substrate-binding protein, at 1,257,461 to 1,258,597
_RS05995
BWI76_RS06000: BWI76_RS06000 - ethanolamine ammonia-lyase small subunit, at 1,258,964 to 1,259,740
_RS06000
BWI76_RS06005: BWI76_RS06005 - ethanolamine ammonia lyase large subunit, at 1,259,737 to 1,261,125
_RS06005
Position (kb) 1258
1259
1260 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1258.192 kb on - strand, within BWI76_RS05995 at 1258.345 kb on - strand, within BWI76_RS05995 at 1258.384 kb on + strand, within BWI76_RS05995 at 1258.390 kb on + strand, within BWI76_RS05995 at 1258.420 kb on + strand, within BWI76_RS05995 at 1258.435 kb on - strand, within BWI76_RS05995 at 1258.439 kb on + strand, within BWI76_RS05995 at 1258.458 kb on + strand, within BWI76_RS05995 at 1258.467 kb on + strand, within BWI76_RS05995 at 1258.470 kb on - strand, within BWI76_RS05995 at 1258.470 kb on - strand, within BWI76_RS05995 at 1258.470 kb on - strand, within BWI76_RS05995 at 1258.477 kb on + strand, within BWI76_RS05995 at 1258.492 kb on - strand at 1258.504 kb on - strand at 1258.530 kb on - strand at 1258.533 kb on - strand at 1258.536 kb on + strand at 1258.536 kb on + strand at 1258.536 kb on + strand at 1258.536 kb on + strand at 1258.536 kb on + strand at 1258.544 kb on - strand at 1258.544 kb on - strand at 1258.544 kb on - strand at 1258.544 kb on - strand at 1258.544 kb on - strand at 1258.556 kb on - strand at 1258.603 kb on + strand at 1258.635 kb on + strand at 1258.635 kb on + strand at 1258.643 kb on + strand at 1258.684 kb on + strand at 1258.699 kb on + strand at 1258.702 kb on - strand at 1258.705 kb on - strand at 1258.740 kb on - strand at 1258.794 kb on + strand at 1258.794 kb on + strand at 1258.794 kb on + strand at 1258.794 kb on + strand at 1258.795 kb on + strand at 1258.796 kb on + strand at 1258.796 kb on + strand at 1258.796 kb on + strand at 1258.796 kb on + strand at 1258.796 kb on + strand at 1258.796 kb on + strand at 1258.796 kb on + strand at 1258.796 kb on + strand at 1258.796 kb on + strand at 1258.802 kb on - strand at 1258.802 kb on - strand at 1258.803 kb on + strand at 1258.803 kb on + strand at 1258.803 kb on - strand at 1258.804 kb on - strand at 1258.804 kb on - strand at 1258.804 kb on - strand at 1258.804 kb on - strand at 1258.804 kb on - strand at 1258.804 kb on - strand at 1258.804 kb on - strand at 1258.806 kb on + strand at 1258.806 kb on + strand at 1258.808 kb on + strand at 1258.808 kb on + strand at 1258.813 kb on + strand at 1258.814 kb on - strand at 1258.816 kb on - strand at 1258.816 kb on - strand at 1258.816 kb on - strand at 1258.816 kb on - strand at 1258.816 kb on - strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.829 kb on + strand at 1258.854 kb on + strand at 1258.854 kb on + strand at 1258.854 kb on + strand at 1258.854 kb on + strand at 1258.860 kb on + strand at 1258.861 kb on + strand at 1258.862 kb on - strand at 1258.872 kb on - strand at 1258.877 kb on - strand at 1258.886 kb on + strand at 1258.894 kb on - strand at 1258.894 kb on - strand at 1258.894 kb on - strand at 1258.894 kb on - strand at 1258.894 kb on - strand at 1258.894 kb on - strand at 1258.894 kb on - strand at 1258.905 kb on - strand at 1258.905 kb on - strand at 1258.925 kb on - strand at 1258.984 kb on - strand at 1259.002 kb on + strand at 1259.002 kb on + strand at 1259.002 kb on + strand at 1259.002 kb on + strand at 1259.002 kb on + strand at 1259.002 kb on + strand at 1259.002 kb on + strand at 1259.002 kb on + strand at 1259.010 kb on - strand at 1259.010 kb on - strand at 1259.010 kb on - strand at 1259.010 kb on - strand at 1259.010 kb on - strand at 1259.010 kb on - strand at 1259.010 kb on - strand at 1259.022 kb on + strand at 1259.022 kb on + strand at 1259.035 kb on + strand at 1259.037 kb on + strand at 1259.040 kb on - strand at 1259.041 kb on - strand at 1259.043 kb on - strand, within BWI76_RS06000 at 1259.043 kb on - strand, within BWI76_RS06000 at 1259.043 kb on - strand, within BWI76_RS06000 at 1259.043 kb on - strand, within BWI76_RS06000 at 1259.043 kb on - strand, within BWI76_RS06000 at 1259.043 kb on - strand, within BWI76_RS06000 at 1259.045 kb on - strand, within BWI76_RS06000 at 1259.057 kb on + strand, within BWI76_RS06000 at 1259.057 kb on + strand at 1259.057 kb on + strand, within BWI76_RS06000 at 1259.060 kb on - strand, within BWI76_RS06000 at 1259.065 kb on - strand, within BWI76_RS06000 at 1259.065 kb on - strand, within BWI76_RS06000 at 1259.070 kb on + strand, within BWI76_RS06000 at 1259.078 kb on - strand, within BWI76_RS06000 at 1259.078 kb on - strand, within BWI76_RS06000 at 1259.078 kb on - strand, within BWI76_RS06000 at 1259.078 kb on - strand at 1259.078 kb on - strand, within BWI76_RS06000 at 1259.080 kb on + strand, within BWI76_RS06000 at 1259.080 kb on + strand, within BWI76_RS06000 at 1259.080 kb on + strand, within BWI76_RS06000 at 1259.080 kb on + strand, within BWI76_RS06000 at 1259.080 kb on + strand, within BWI76_RS06000 at 1259.080 kb on + strand, within BWI76_RS06000 at 1259.080 kb on + strand, within BWI76_RS06000 at 1259.080 kb on + strand, within BWI76_RS06000 at 1259.080 kb on + strand, within BWI76_RS06000 at 1259.088 kb on - strand, within BWI76_RS06000 at 1259.088 kb on - strand, within BWI76_RS06000 at 1259.088 kb on - strand, within BWI76_RS06000 at 1259.088 kb on - strand, within BWI76_RS06000 at 1259.095 kb on + strand, within BWI76_RS06000 at 1259.103 kb on - strand, within BWI76_RS06000 at 1259.109 kb on - strand, within BWI76_RS06000 at 1259.110 kb on - strand, within BWI76_RS06000 at 1259.153 kb on - strand, within BWI76_RS06000 at 1259.160 kb on + strand, within BWI76_RS06000 at 1259.183 kb on - strand, within BWI76_RS06000 at 1259.183 kb on - strand, within BWI76_RS06000 at 1259.184 kb on + strand, within BWI76_RS06000 at 1259.185 kb on + strand, within BWI76_RS06000 at 1259.193 kb on - strand, within BWI76_RS06000 at 1259.193 kb on - strand, within BWI76_RS06000 at 1259.193 kb on - strand, within BWI76_RS06000 at 1259.193 kb on - strand, within BWI76_RS06000 at 1259.203 kb on - strand, within BWI76_RS06000 at 1259.207 kb on - strand, within BWI76_RS06000 at 1259.241 kb on + strand, within BWI76_RS06000 at 1259.249 kb on - strand, within BWI76_RS06000 at 1259.249 kb on - strand, within BWI76_RS06000 at 1259.249 kb on - strand, within BWI76_RS06000 at 1259.249 kb on - strand, within BWI76_RS06000 at 1259.313 kb on - strand, within BWI76_RS06000 at 1259.350 kb on - strand, within BWI76_RS06000 at 1259.375 kb on + strand, within BWI76_RS06000 at 1259.385 kb on + strand, within BWI76_RS06000 at 1259.442 kb on - strand, within BWI76_RS06000 at 1259.662 kb on + strand, within BWI76_RS06000 at 1259.743 kb on - strand at 1259.964 kb on + strand, within BWI76_RS06005 at 1259.972 kb on + strand, within BWI76_RS06005 at 1260.012 kb on - strand, within BWI76_RS06005 at 1260.047 kb on - strand, within BWI76_RS06005 at 1260.261 kb on - strand, within BWI76_RS06005 at 1260.468 kb on + strand, within BWI76_RS06005 at 1260.500 kb on - strand, within BWI76_RS06005 at 1260.517 kb on - strand, within BWI76_RS06005 at 1260.517 kb on - strand, within BWI76_RS06005 at 1260.557 kb on - strand, within BWI76_RS06005 at 1260.578 kb on + strand, within BWI76_RS06005 at 1260.662 kb on + strand, within BWI76_RS06005
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 1,258,192 - BWI76_RS05995 0.64 -0.5 1,258,345 - BWI76_RS05995 0.78 +0.1 1,258,384 + BWI76_RS05995 0.81 -0.1 1,258,390 + BWI76_RS05995 0.82 -0.6 1,258,420 + BWI76_RS05995 0.84 +0.4 1,258,435 - BWI76_RS05995 0.86 +0.2 1,258,439 + BWI76_RS05995 0.86 -1.0 1,258,458 + BWI76_RS05995 0.88 -0.2 1,258,467 + BWI76_RS05995 0.88 -0.0 1,258,470 - BWI76_RS05995 0.89 -0.6 1,258,470 - BWI76_RS05995 0.89 -0.0 1,258,470 - BWI76_RS05995 0.89 +0.1 1,258,477 + BWI76_RS05995 0.89 -0.0 1,258,492 - +0.1 1,258,504 - +0.3 1,258,530 - +0.0 1,258,533 - -0.7 1,258,536 + -0.7 1,258,536 + +0.3 1,258,536 + -0.1 1,258,536 + +0.7 1,258,536 + +0.3 1,258,544 - -0.1 1,258,544 - +1.0 1,258,544 - +1.0 1,258,544 - -0.2 1,258,544 - -1.2 1,258,556 - -0.0 1,258,603 + +0.2 1,258,635 + -0.8 1,258,635 + -0.4 1,258,643 + -0.2 1,258,684 + -0.3 1,258,699 + -1.5 1,258,702 - -0.5 1,258,705 - +0.2 1,258,740 - +0.1 1,258,794 + +1.1 1,258,794 + +0.1 1,258,794 + -0.9 1,258,794 + +0.9 1,258,795 + -0.0 1,258,796 + +0.3 1,258,796 + +0.3 1,258,796 + -0.8 1,258,796 + +0.4 1,258,796 + -1.8 1,258,796 + +0.5 1,258,796 + +0.2 1,258,796 + +0.0 1,258,796 + +0.3 1,258,802 - -0.3 1,258,802 - +0.1 1,258,803 + +0.4 1,258,803 + -0.4 1,258,803 - -0.1 1,258,804 - +0.8 1,258,804 - -1.0 1,258,804 - -0.1 1,258,804 - +0.3 1,258,804 - -0.2 1,258,804 - +0.8 1,258,804 - +0.4 1,258,806 + -0.3 1,258,806 + +0.1 1,258,808 + +0.8 1,258,808 + +0.1 1,258,813 + +0.0 1,258,814 - +1.6 1,258,816 - +0.2 1,258,816 - +0.5 1,258,816 - -0.4 1,258,816 - +0.4 1,258,816 - -0.1 1,258,818 + -0.9 1,258,818 + -0.0 1,258,818 + -0.0 1,258,818 + +0.2 1,258,818 + +1.0 1,258,818 + +0.3 1,258,818 + -0.1 1,258,818 + -0.5 1,258,818 + +0.4 1,258,818 + -0.1 1,258,818 + -0.0 1,258,818 + -0.3 1,258,818 + -0.2 1,258,826 - -0.1 1,258,826 - +0.2 1,258,826 - -1.0 1,258,826 - +0.7 1,258,826 - -0.6 1,258,826 - +0.4 1,258,826 - +0.1 1,258,826 - -0.0 1,258,826 - -1.0 1,258,826 - -0.5 1,258,826 - +0.5 1,258,826 - -0.1 1,258,826 - +0.4 1,258,826 - +0.6 1,258,826 - +0.3 1,258,826 - -0.4 1,258,826 - +0.3 1,258,826 - -0.4 1,258,829 + -0.5 1,258,854 + +1.0 1,258,854 + +0.2 1,258,854 + -0.9 1,258,854 + -0.0 1,258,860 + +0.1 1,258,861 + -0.3 1,258,862 - -0.3 1,258,872 - +0.4 1,258,877 - -0.6 1,258,886 + -0.2 1,258,894 - -0.6 1,258,894 - -0.0 1,258,894 - +0.5 1,258,894 - +0.0 1,258,894 - +0.4 1,258,894 - -0.6 1,258,894 - +0.1 1,258,905 - +0.4 1,258,905 - +0.1 1,258,925 - -0.7 1,258,984 - -0.0 1,259,002 + -0.4 1,259,002 + -0.1 1,259,002 + -0.1 1,259,002 + -0.2 1,259,002 + -0.0 1,259,002 + -0.8 1,259,002 + +0.2 1,259,002 + +0.3 1,259,010 - -0.1 1,259,010 - -0.1 1,259,010 - -0.1 1,259,010 - -0.1 1,259,010 - -0.4 1,259,010 - +0.2 1,259,010 - +0.2 1,259,022 + -0.2 1,259,022 + -0.2 1,259,035 + -0.1 1,259,037 + +0.2 1,259,040 - -0.2 1,259,041 - +0.0 1,259,043 - BWI76_RS06000 0.10 -0.2 1,259,043 - BWI76_RS06000 0.10 -0.0 1,259,043 - BWI76_RS06000 0.10 +1.6 1,259,043 - BWI76_RS06000 0.10 -0.0 1,259,043 - BWI76_RS06000 0.10 -0.1 1,259,043 - BWI76_RS06000 0.10 -0.4 1,259,045 - BWI76_RS06000 0.10 -0.9 1,259,057 + BWI76_RS06000 0.12 -0.1 1,259,057 + -1.2 1,259,057 + BWI76_RS06000 0.12 -0.7 1,259,060 - BWI76_RS06000 0.12 +0.2 1,259,065 - BWI76_RS06000 0.13 -0.4 1,259,065 - BWI76_RS06000 0.13 -0.1 1,259,070 + BWI76_RS06000 0.14 -0.3 1,259,078 - BWI76_RS06000 0.15 +0.1 1,259,078 - BWI76_RS06000 0.15 -0.2 1,259,078 - BWI76_RS06000 0.15 -0.5 1,259,078 - -1.6 1,259,078 - BWI76_RS06000 0.15 +0.3 1,259,080 + BWI76_RS06000 0.15 -3.4 1,259,080 + BWI76_RS06000 0.15 +0.4 1,259,080 + BWI76_RS06000 0.15 -0.2 1,259,080 + BWI76_RS06000 0.15 +0.3 1,259,080 + BWI76_RS06000 0.15 +0.4 1,259,080 + BWI76_RS06000 0.15 +0.6 1,259,080 + BWI76_RS06000 0.15 -0.5 1,259,080 + BWI76_RS06000 0.15 +0.1 1,259,080 + BWI76_RS06000 0.15 -0.1 1,259,088 - BWI76_RS06000 0.16 +0.2 1,259,088 - BWI76_RS06000 0.16 +0.5 1,259,088 - BWI76_RS06000 0.16 +0.1 1,259,088 - BWI76_RS06000 0.16 +0.9 1,259,095 + BWI76_RS06000 0.17 -0.1 1,259,103 - BWI76_RS06000 0.18 -0.7 1,259,109 - BWI76_RS06000 0.19 -0.9 1,259,110 - BWI76_RS06000 0.19 +0.4 1,259,153 - BWI76_RS06000 0.24 +0.5 1,259,160 + BWI76_RS06000 0.25 -0.1 1,259,183 - BWI76_RS06000 0.28 -0.0 1,259,183 - BWI76_RS06000 0.28 -0.1 1,259,184 + BWI76_RS06000 0.28 +0.8 1,259,185 + BWI76_RS06000 0.28 +1.2 1,259,193 - BWI76_RS06000 0.29 +0.4 1,259,193 - BWI76_RS06000 0.29 +0.8 1,259,193 - BWI76_RS06000 0.29 +0.8 1,259,193 - BWI76_RS06000 0.29 -0.3 1,259,203 - BWI76_RS06000 0.31 -0.3 1,259,207 - BWI76_RS06000 0.31 +0.0 1,259,241 + BWI76_RS06000 0.36 +0.0 1,259,249 - BWI76_RS06000 0.37 -0.4 1,259,249 - BWI76_RS06000 0.37 +0.0 1,259,249 - BWI76_RS06000 0.37 -0.4 1,259,249 - BWI76_RS06000 0.37 -0.8 1,259,313 - BWI76_RS06000 0.45 +0.0 1,259,350 - BWI76_RS06000 0.50 +0.4 1,259,375 + BWI76_RS06000 0.53 -0.1 1,259,385 + BWI76_RS06000 0.54 -0.8 1,259,442 - BWI76_RS06000 0.62 +0.2 1,259,662 + BWI76_RS06000 0.90 +0.1 1,259,743 - -0.1 1,259,964 + BWI76_RS06005 0.16 +0.1 1,259,972 + BWI76_RS06005 0.17 +0.1 1,260,012 - BWI76_RS06005 0.20 -0.9 1,260,047 - BWI76_RS06005 0.22 -0.4 1,260,261 - BWI76_RS06005 0.38 -0.3 1,260,468 + BWI76_RS06005 0.53 -0.3 1,260,500 - BWI76_RS06005 0.55 +0.8 1,260,517 - BWI76_RS06005 0.56 -0.0 1,260,517 - BWI76_RS06005 0.56 -0.4 1,260,557 - BWI76_RS06005 0.59 -1.5 1,260,578 + BWI76_RS06005 0.61 -0.3 1,260,662 + BWI76_RS06005 0.67 +0.2
Or see this region's nucleotide sequence