Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04965 and BWI76_RS04970 overlap by 4 nucleotides BWI76_RS04970 and BWI76_RS04975 are separated by 121 nucleotides BWI76_RS04975 and BWI76_RS04980 are separated by 57 nucleotides
BWI76_RS04965: BWI76_RS04965 - ABC transporter ATP-binding protein, at 1,042,941 to 1,043,867
_RS04965
BWI76_RS04970: BWI76_RS04970 - ABC transporter permease, at 1,043,864 to 1,044,634
_RS04970
BWI76_RS04975: BWI76_RS04975 - PTS fructose subfamily transporter subunit IIA, at 1,044,756 to 1,045,196
_RS04975
BWI76_RS04980: BWI76_RS04980 - hypothetical protein, at 1,045,254 to 1,046,507
_RS04980
Position (kb) 1043
1044
1045 Strain fitness (log2 ratio) -1
0
1 at 1043.048 kb on + strand, within BWI76_RS04965 at 1043.131 kb on - strand, within BWI76_RS04965 at 1043.190 kb on - strand, within BWI76_RS04965 at 1043.524 kb on + strand, within BWI76_RS04965 at 1043.532 kb on - strand, within BWI76_RS04965 at 1043.560 kb on + strand, within BWI76_RS04965 at 1043.784 kb on - strand at 1044.143 kb on - strand, within BWI76_RS04970 at 1044.297 kb on + strand, within BWI76_RS04970 at 1044.305 kb on - strand, within BWI76_RS04970 at 1044.654 kb on + strand at 1044.842 kb on + strand, within BWI76_RS04975 at 1044.959 kb on - strand, within BWI76_RS04975 at 1045.356 kb on - strand at 1045.436 kb on - strand, within BWI76_RS04980 at 1045.514 kb on - strand, within BWI76_RS04980
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 1,043,048 + BWI76_RS04965 0.12 +0.0 1,043,131 - BWI76_RS04965 0.20 -0.4 1,043,190 - BWI76_RS04965 0.27 -0.1 1,043,524 + BWI76_RS04965 0.63 -0.0 1,043,532 - BWI76_RS04965 0.64 +0.1 1,043,560 + BWI76_RS04965 0.67 -1.4 1,043,784 - -0.1 1,044,143 - BWI76_RS04970 0.36 +0.0 1,044,297 + BWI76_RS04970 0.56 +1.0 1,044,305 - BWI76_RS04970 0.57 +1.2 1,044,654 + +0.1 1,044,842 + BWI76_RS04975 0.20 +0.1 1,044,959 - BWI76_RS04975 0.46 -0.1 1,045,356 - +0.5 1,045,436 - BWI76_RS04980 0.15 +0.2 1,045,514 - BWI76_RS04980 0.21 -0.3
Or see this region's nucleotide sequence