Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04060 and BWI76_RS04065 are separated by 20 nucleotides BWI76_RS04065 and BWI76_RS04070 are separated by 31 nucleotides BWI76_RS04070 and BWI76_RS04075 are separated by 28 nucleotides BWI76_RS04075 and BWI76_RS04080 are separated by 167 nucleotides BWI76_RS04080 and BWI76_RS04085 are separated by 158 nucleotides BWI76_RS04085 and BWI76_RS04090 overlap by 32 nucleotides
BWI76_RS04060: BWI76_RS04060 - hypothetical protein, at 839,511 to 839,750
_RS04060
BWI76_RS04065: BWI76_RS04065 - tRNA-Leu, at 839,771 to 839,857
_RS04065
BWI76_RS04070: BWI76_RS04070 - tRNA-Leu, at 839,889 to 839,975
_RS04070
BWI76_RS04075: BWI76_RS04075 - tRNA-Leu, at 840,004 to 840,090
_RS04075
BWI76_RS04080: BWI76_RS04080 - 16S rRNA (guanine(1207)-N(2))-methyltransferase, at 840,258 to 841,286
_RS04080
BWI76_RS04085: BWI76_RS04085 - DNA polymerase III subunit psi, at 841,445 to 841,858
_RS04085
BWI76_RS04090: BWI76_RS04090 - ribosomal-protein-alanine N-acetyltransferase RimI, at 841,827 to 842,273
_RS04090
Position (kb) 840
841
842 Strain fitness (log2 ratio) -2
-1
0
1 at 839.289 kb on - strand at 839.292 kb on + strand at 839.386 kb on + strand at 839.398 kb on + strand at 839.422 kb on + strand at 839.422 kb on + strand at 839.422 kb on + strand at 839.422 kb on + strand at 839.430 kb on - strand at 839.430 kb on - strand at 839.430 kb on - strand at 839.531 kb on - strand at 839.555 kb on - strand, within BWI76_RS04060 at 839.638 kb on - strand, within BWI76_RS04060 at 839.697 kb on + strand, within BWI76_RS04060 at 840.292 kb on - strand at 840.764 kb on - strand, within BWI76_RS04080 at 840.808 kb on + strand, within BWI76_RS04080 at 840.808 kb on + strand, within BWI76_RS04080 at 840.816 kb on - strand, within BWI76_RS04080 at 840.816 kb on - strand, within BWI76_RS04080 at 840.863 kb on + strand, within BWI76_RS04080 at 840.930 kb on + strand, within BWI76_RS04080 at 840.933 kb on - strand, within BWI76_RS04080 at 840.938 kb on - strand, within BWI76_RS04080 at 840.966 kb on + strand, within BWI76_RS04080 at 841.008 kb on + strand, within BWI76_RS04080 at 841.021 kb on + strand, within BWI76_RS04080 at 841.124 kb on + strand, within BWI76_RS04080 at 841.173 kb on + strand, within BWI76_RS04080 at 841.271 kb on + strand at 841.857 kb on + strand at 841.983 kb on + strand, within BWI76_RS04090 at 842.066 kb on + strand, within BWI76_RS04090 at 842.076 kb on - strand, within BWI76_RS04090 at 842.128 kb on + strand, within BWI76_RS04090 at 842.143 kb on + strand, within BWI76_RS04090
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 839,289 - +0.5 839,292 + +0.3 839,386 + +0.0 839,398 + +0.4 839,422 + +0.3 839,422 + +0.9 839,422 + +1.1 839,422 + +0.2 839,430 - +0.2 839,430 - +0.8 839,430 - -0.1 839,531 - +0.4 839,555 - BWI76_RS04060 0.18 +0.1 839,638 - BWI76_RS04060 0.53 +0.7 839,697 + BWI76_RS04060 0.78 -0.5 840,292 - -0.2 840,764 - BWI76_RS04080 0.49 -0.2 840,808 + BWI76_RS04080 0.53 -0.4 840,808 + BWI76_RS04080 0.53 -0.5 840,816 - BWI76_RS04080 0.54 +0.3 840,816 - BWI76_RS04080 0.54 -0.2 840,863 + BWI76_RS04080 0.59 +0.8 840,930 + BWI76_RS04080 0.65 -0.4 840,933 - BWI76_RS04080 0.66 +0.0 840,938 - BWI76_RS04080 0.66 -0.3 840,966 + BWI76_RS04080 0.69 -0.1 841,008 + BWI76_RS04080 0.73 +0.4 841,021 + BWI76_RS04080 0.74 -2.0 841,124 + BWI76_RS04080 0.84 -0.2 841,173 + BWI76_RS04080 0.89 +0.6 841,271 + -0.1 841,857 + +0.2 841,983 + BWI76_RS04090 0.35 +0.0 842,066 + BWI76_RS04090 0.53 -0.2 842,076 - BWI76_RS04090 0.56 +0.8 842,128 + BWI76_RS04090 0.67 -0.5 842,143 + BWI76_RS04090 0.71 +0.3
Or see this region's nucleotide sequence