Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00700 and BWI76_RS00705 are separated by 291 nucleotides BWI76_RS00705 and BWI76_RS00710 are separated by 71 nucleotides BWI76_RS00710 and BWI76_RS00715 are separated by 91 nucleotides
BWI76_RS00700: BWI76_RS00700 - rhamnose/proton symporter RhaT, at 142,745 to 143,779
_RS00700
BWI76_RS00705: BWI76_RS00705 - superoxide dismutase, at 144,071 to 144,691
_RS00705
BWI76_RS00710: BWI76_RS00710 - 6-N-hydroxylaminopurine resistance protein, at 144,763 to 145,437
_RS00710
BWI76_RS00715: BWI76_RS00715 - two-component sensor histidine kinase, at 145,529 to 146,902
_RS00715
Position (kb) 144
145
146 Strain fitness (log2 ratio) -1
0
1 at 143.785 kb on - strand at 143.823 kb on + strand at 143.866 kb on - strand at 143.866 kb on - strand at 143.866 kb on - strand at 143.880 kb on + strand at 143.948 kb on + strand at 144.031 kb on + strand at 144.704 kb on - strand at 144.802 kb on - strand at 145.079 kb on + strand, within BWI76_RS00710 at 145.087 kb on - strand, within BWI76_RS00710 at 145.478 kb on + strand at 145.560 kb on + strand at 145.686 kb on - strand, within BWI76_RS00715 at 146.062 kb on + strand, within BWI76_RS00715 at 146.068 kb on + strand, within BWI76_RS00715 at 146.146 kb on - strand at 146.232 kb on - strand, within BWI76_RS00715 at 146.232 kb on - strand, within BWI76_RS00715 at 146.345 kb on - strand, within BWI76_RS00715 at 146.348 kb on + strand, within BWI76_RS00715
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 143,785 - -1.2 143,823 + +0.1 143,866 - -0.2 143,866 - -0.3 143,866 - +0.7 143,880 + +0.2 143,948 + -0.1 144,031 + -0.2 144,704 - +0.8 144,802 - -0.1 145,079 + BWI76_RS00710 0.47 -1.1 145,087 - BWI76_RS00710 0.48 -0.1 145,478 + +0.3 145,560 + +0.2 145,686 - BWI76_RS00715 0.11 +0.3 146,062 + BWI76_RS00715 0.39 +0.8 146,068 + BWI76_RS00715 0.39 +1.1 146,146 - +0.1 146,232 - BWI76_RS00715 0.51 +1.1 146,232 - BWI76_RS00715 0.51 +0.2 146,345 - BWI76_RS00715 0.59 +0.9 146,348 + BWI76_RS00715 0.60 +0.8
Or see this region's nucleotide sequence