Experiment: MoLS4 with N2 gas (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cheW-2 and DVU1905 are separated by 224 nucleotides DVU1905 and ugd are separated by 374 nucleotides ugd and pdxJ are separated by 151 nucleotides pdxJ and acpS are separated by 10 nucleotides
DVU1904: cheW-2 - chemotaxis protein CheW (TIGR), at 1,980,940 to 1,981,416
cheW-2
DVU1905: DVU1905 - hypothetical protein (TIGR), at 1,981,641 to 1,981,865
DVU1905
DVU1907: ugd - UDP-glucose 6-dehydrogenase (TIGR), at 1,982,240 to 1,983,577
ugd
DVU1908: pdxJ - pyridoxal phosphate biosynthetic protein PdxJ (TIGR), at 1,983,729 to 1,984,454
pdxJ
DVU1909: acpS - holo-(acyl-carrier-protein) synthase (TIGR), at 1,984,465 to 1,984,839
acpS
Position (kb)
1982
1983
1984 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1981.287 kb on - strand, within cheW-2 at 1981.347 kb on + strand, within cheW-2 at 1981.399 kb on + strand at 1981.399 kb on + strand at 1981.399 kb on + strand at 1981.418 kb on + strand at 1981.426 kb on - strand at 1981.426 kb on - strand at 1981.492 kb on - strand at 1981.496 kb on + strand at 1981.547 kb on + strand at 1981.550 kb on - strand at 1981.677 kb on - strand, within DVU1905 at 1981.846 kb on + strand at 1981.948 kb on - strand at 1981.980 kb on - strand at 1982.063 kb on - strand at 1982.165 kb on + strand at 1983.589 kb on - strand at 1983.685 kb on + strand at 1983.688 kb on - strand at 1983.725 kb on + strand at 1983.884 kb on + strand, within pdxJ at 1984.186 kb on + strand, within pdxJ at 1984.255 kb on + strand, within pdxJ
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with N2 gas (N) remove 1,981,287 - cheW-2 DVU1904 0.73 -1.6 1,981,347 + cheW-2 DVU1904 0.85 -0.8 1,981,399 + -2.8 1,981,399 + -3.2 1,981,399 + -3.8 1,981,418 + -1.9 1,981,426 - -1.1 1,981,426 - -2.2 1,981,492 - +0.5 1,981,496 + -0.6 1,981,547 + -1.3 1,981,550 - -2.4 1,981,677 - DVU1905 0.16 -1.6 1,981,846 + -0.9 1,981,948 - +0.1 1,981,980 - +0.8 1,982,063 - +0.4 1,982,165 + -0.4 1,983,589 - -0.1 1,983,685 + +1.8 1,983,688 - +0.4 1,983,725 + +0.9 1,983,884 + pdxJ DVU1908 0.21 +0.1 1,984,186 + pdxJ DVU1908 0.63 -0.2 1,984,255 + pdxJ DVU1908 0.72 -0.6
Or see this region's nucleotide sequence