Experiment: BG11 with nitrate 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfbA and Synpcc7942_2102 are separated by 11 nucleotides Synpcc7942_2102 and Synpcc7942_2103 are separated by 42 nucleotides Synpcc7942_2103 and cynS are separated by 216 nucleotides cynS and cynD are separated by 10 nucleotides
Synpcc7942_2101: rfbA - glucose-1-phosphate thymidylyltransferase, at 2,182,941 to 2,183,825
rfbA
Synpcc7942_2102: Synpcc7942_2102 - NAD-reducing hydrogenase gamma, at 2,183,837 to 2,184,076
_2102
Synpcc7942_2103: Synpcc7942_2103 - hypothetical protein, at 2,184,119 to 2,184,523
_2103
Synpcc7942_2104: cynS - cyanate hydratase, at 2,184,740 to 2,185,180
cynS
Synpcc7942_2105: cynD - nitrate transport ATP-binding subunits C and D, at 2,185,191 to 2,186,060
cynD
Position (kb)
2184
2185 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2183.123 kb on - strand, within rfbA at 2183.123 kb on - strand, within rfbA at 2183.141 kb on + strand, within rfbA at 2183.177 kb on + strand, within rfbA at 2183.177 kb on + strand, within rfbA at 2183.207 kb on - strand, within rfbA at 2183.214 kb on - strand, within rfbA at 2183.238 kb on + strand, within rfbA at 2183.338 kb on + strand, within rfbA at 2183.383 kb on - strand, within rfbA at 2183.432 kb on + strand, within rfbA at 2183.432 kb on + strand, within rfbA at 2183.457 kb on + strand, within rfbA at 2183.466 kb on + strand, within rfbA at 2183.501 kb on + strand, within rfbA at 2183.513 kb on - strand, within rfbA at 2183.540 kb on - strand, within rfbA at 2183.549 kb on + strand, within rfbA at 2183.557 kb on - strand, within rfbA at 2183.574 kb on + strand, within rfbA at 2183.592 kb on - strand, within rfbA at 2183.609 kb on + strand, within rfbA at 2183.628 kb on + strand, within rfbA at 2183.628 kb on + strand, within rfbA at 2183.636 kb on - strand, within rfbA at 2183.636 kb on - strand, within rfbA at 2183.721 kb on + strand, within rfbA at 2183.740 kb on - strand at 2183.746 kb on + strand at 2183.754 kb on - strand at 2183.763 kb on + strand at 2183.771 kb on - strand at 2183.804 kb on - strand at 2183.813 kb on - strand at 2183.827 kb on - strand at 2183.841 kb on + strand at 2183.849 kb on + strand at 2183.849 kb on - strand at 2183.862 kb on + strand, within Synpcc7942_2102 at 2183.897 kb on + strand, within Synpcc7942_2102 at 2183.905 kb on - strand, within Synpcc7942_2102 at 2183.905 kb on - strand, within Synpcc7942_2102 at 2183.905 kb on - strand, within Synpcc7942_2102 at 2183.905 kb on - strand, within Synpcc7942_2102 at 2183.924 kb on + strand, within Synpcc7942_2102 at 2183.932 kb on - strand, within Synpcc7942_2102 at 2183.946 kb on + strand, within Synpcc7942_2102 at 2183.958 kb on + strand, within Synpcc7942_2102 at 2183.962 kb on - strand, within Synpcc7942_2102 at 2183.966 kb on - strand, within Synpcc7942_2102 at 2183.996 kb on + strand, within Synpcc7942_2102 at 2184.034 kb on + strand, within Synpcc7942_2102 at 2184.035 kb on + strand, within Synpcc7942_2102 at 2184.076 kb on + strand at 2184.097 kb on - strand at 2184.141 kb on + strand at 2184.168 kb on + strand, within Synpcc7942_2103 at 2184.170 kb on - strand, within Synpcc7942_2103 at 2184.176 kb on - strand, within Synpcc7942_2103 at 2184.186 kb on - strand, within Synpcc7942_2103 at 2184.225 kb on - strand, within Synpcc7942_2103 at 2184.305 kb on - strand, within Synpcc7942_2103 at 2184.324 kb on + strand, within Synpcc7942_2103 at 2184.335 kb on + strand, within Synpcc7942_2103 at 2184.345 kb on + strand, within Synpcc7942_2103 at 2184.353 kb on + strand, within Synpcc7942_2103 at 2184.408 kb on - strand, within Synpcc7942_2103 at 2184.426 kb on + strand, within Synpcc7942_2103 at 2184.434 kb on - strand, within Synpcc7942_2103 at 2184.468 kb on - strand, within Synpcc7942_2103 at 2184.518 kb on + strand at 2184.528 kb on - strand at 2184.562 kb on + strand at 2184.576 kb on - strand at 2184.604 kb on + strand at 2184.606 kb on + strand at 2184.606 kb on + strand at 2184.607 kb on - strand at 2184.612 kb on - strand at 2184.624 kb on - strand at 2184.624 kb on - strand at 2184.631 kb on + strand at 2184.634 kb on - strand at 2184.639 kb on - strand at 2184.696 kb on + strand at 2184.764 kb on - strand at 2184.764 kb on - strand at 2184.795 kb on + strand, within cynS at 2184.893 kb on + strand, within cynS at 2184.894 kb on + strand, within cynS at 2184.894 kb on + strand, within cynS at 2184.902 kb on - strand, within cynS at 2184.962 kb on + strand, within cynS at 2184.971 kb on - strand, within cynS at 2184.986 kb on + strand, within cynS at 2185.001 kb on - strand, within cynS at 2185.010 kb on + strand, within cynS at 2185.188 kb on + strand at 2185.190 kb on + strand at 2185.298 kb on - strand, within cynD at 2185.312 kb on + strand, within cynD at 2185.361 kb on + strand, within cynD at 2185.385 kb on + strand, within cynD at 2185.388 kb on - strand, within cynD at 2185.399 kb on - strand, within cynD at 2185.420 kb on - strand, within cynD at 2185.468 kb on + strand, within cynD at 2185.473 kb on + strand, within cynD at 2185.491 kb on + strand, within cynD
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with nitrate 25 mM remove 2,183,123 - rfbA Synpcc7942_2101 0.21 +0.6 2,183,123 - rfbA Synpcc7942_2101 0.21 +0.6 2,183,141 + rfbA Synpcc7942_2101 0.23 +0.4 2,183,177 + rfbA Synpcc7942_2101 0.27 -0.7 2,183,177 + rfbA Synpcc7942_2101 0.27 -0.9 2,183,207 - rfbA Synpcc7942_2101 0.30 -1.3 2,183,214 - rfbA Synpcc7942_2101 0.31 -0.4 2,183,238 + rfbA Synpcc7942_2101 0.34 -1.5 2,183,338 + rfbA Synpcc7942_2101 0.45 -0.4 2,183,383 - rfbA Synpcc7942_2101 0.50 -0.7 2,183,432 + rfbA Synpcc7942_2101 0.55 +0.5 2,183,432 + rfbA Synpcc7942_2101 0.55 -0.3 2,183,457 + rfbA Synpcc7942_2101 0.58 +0.3 2,183,466 + rfbA Synpcc7942_2101 0.59 -1.1 2,183,501 + rfbA Synpcc7942_2101 0.63 +0.4 2,183,513 - rfbA Synpcc7942_2101 0.65 -0.2 2,183,540 - rfbA Synpcc7942_2101 0.68 -1.3 2,183,549 + rfbA Synpcc7942_2101 0.69 -1.3 2,183,557 - rfbA Synpcc7942_2101 0.70 +0.9 2,183,574 + rfbA Synpcc7942_2101 0.72 -0.7 2,183,592 - rfbA Synpcc7942_2101 0.74 -0.0 2,183,609 + rfbA Synpcc7942_2101 0.75 +0.0 2,183,628 + rfbA Synpcc7942_2101 0.78 +0.7 2,183,628 + rfbA Synpcc7942_2101 0.78 -0.5 2,183,636 - rfbA Synpcc7942_2101 0.79 -1.8 2,183,636 - rfbA Synpcc7942_2101 0.79 +0.9 2,183,721 + rfbA Synpcc7942_2101 0.88 -1.4 2,183,740 - +0.1 2,183,746 + -0.3 2,183,754 - +1.1 2,183,763 + +0.1 2,183,771 - +0.2 2,183,804 - -0.0 2,183,813 - -0.0 2,183,827 - +0.6 2,183,841 + -1.0 2,183,849 + -0.9 2,183,849 - -1.1 2,183,862 + Synpcc7942_2102 0.10 -2.6 2,183,897 + Synpcc7942_2102 0.25 -0.3 2,183,905 - Synpcc7942_2102 0.28 -2.3 2,183,905 - Synpcc7942_2102 0.28 -1.4 2,183,905 - Synpcc7942_2102 0.28 -2.9 2,183,905 - Synpcc7942_2102 0.28 -1.4 2,183,924 + Synpcc7942_2102 0.36 -0.9 2,183,932 - Synpcc7942_2102 0.40 +0.9 2,183,946 + Synpcc7942_2102 0.45 -0.5 2,183,958 + Synpcc7942_2102 0.50 -0.8 2,183,962 - Synpcc7942_2102 0.52 -0.5 2,183,966 - Synpcc7942_2102 0.54 -1.7 2,183,996 + Synpcc7942_2102 0.66 -1.6 2,184,034 + Synpcc7942_2102 0.82 +0.1 2,184,035 + Synpcc7942_2102 0.82 -1.1 2,184,076 + -0.1 2,184,097 - -0.9 2,184,141 + +0.5 2,184,168 + Synpcc7942_2103 0.12 -0.9 2,184,170 - Synpcc7942_2103 0.13 +0.1 2,184,176 - Synpcc7942_2103 0.14 -0.6 2,184,186 - Synpcc7942_2103 0.17 -0.4 2,184,225 - Synpcc7942_2103 0.26 -1.4 2,184,305 - Synpcc7942_2103 0.46 +0.9 2,184,324 + Synpcc7942_2103 0.51 -0.3 2,184,335 + Synpcc7942_2103 0.53 -0.4 2,184,345 + Synpcc7942_2103 0.56 +0.5 2,184,353 + Synpcc7942_2103 0.58 -0.8 2,184,408 - Synpcc7942_2103 0.71 -1.4 2,184,426 + Synpcc7942_2103 0.76 -0.2 2,184,434 - Synpcc7942_2103 0.78 -0.1 2,184,468 - Synpcc7942_2103 0.86 -0.4 2,184,518 + -0.5 2,184,528 - -0.5 2,184,562 + -0.1 2,184,576 - +0.5 2,184,604 + +2.1 2,184,606 + -0.7 2,184,606 + +0.4 2,184,607 - +0.1 2,184,612 - -1.1 2,184,624 - +1.3 2,184,624 - +0.2 2,184,631 + -1.5 2,184,634 - +0.2 2,184,639 - -0.2 2,184,696 + -0.3 2,184,764 - -0.4 2,184,764 - -0.3 2,184,795 + cynS Synpcc7942_2104 0.12 +0.9 2,184,893 + cynS Synpcc7942_2104 0.35 -0.3 2,184,894 + cynS Synpcc7942_2104 0.35 -1.6 2,184,894 + cynS Synpcc7942_2104 0.35 -0.0 2,184,902 - cynS Synpcc7942_2104 0.37 -2.5 2,184,962 + cynS Synpcc7942_2104 0.50 +0.6 2,184,971 - cynS Synpcc7942_2104 0.52 +0.2 2,184,986 + cynS Synpcc7942_2104 0.56 +0.1 2,185,001 - cynS Synpcc7942_2104 0.59 -0.8 2,185,010 + cynS Synpcc7942_2104 0.61 -0.3 2,185,188 + -1.6 2,185,190 + -0.9 2,185,298 - cynD Synpcc7942_2105 0.12 -0.4 2,185,312 + cynD Synpcc7942_2105 0.14 -1.3 2,185,361 + cynD Synpcc7942_2105 0.20 +3.1 2,185,385 + cynD Synpcc7942_2105 0.22 +0.5 2,185,388 - cynD Synpcc7942_2105 0.23 +0.2 2,185,399 - cynD Synpcc7942_2105 0.24 -0.5 2,185,420 - cynD Synpcc7942_2105 0.26 +0.9 2,185,468 + cynD Synpcc7942_2105 0.32 -1.2 2,185,473 + cynD Synpcc7942_2105 0.32 +0.4 2,185,491 + cynD Synpcc7942_2105 0.34 -0.3
Or see this region's nucleotide sequence