Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_903 and Pf1N1B4_904 are separated by 207 nucleotides Pf1N1B4_904 and Pf1N1B4_905 are separated by 508 nucleotides
Pf1N1B4_903 - Ribosomal RNA large subunit methyltransferase F (EC 2.1.1.51), at 1,030,756 to 1,031,778
_903
Pf1N1B4_904 - DNA-binding protein HU-beta, at 1,031,986 to 1,032,267
_904
Pf1N1B4_905 - Nucleoid-associated protein, at 1,032,776 to 1,033,780
_905
Position (kb) 1031
1032
1033 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1031.015 kb on - strand, within Pf1N1B4_903 at 1031.047 kb on + strand, within Pf1N1B4_903 at 1031.048 kb on - strand, within Pf1N1B4_903 at 1031.048 kb on - strand, within Pf1N1B4_903 at 1031.080 kb on + strand, within Pf1N1B4_903 at 1031.080 kb on + strand, within Pf1N1B4_903 at 1031.081 kb on - strand, within Pf1N1B4_903 at 1031.081 kb on - strand, within Pf1N1B4_903 at 1031.081 kb on - strand, within Pf1N1B4_903 at 1031.081 kb on - strand, within Pf1N1B4_903 at 1031.081 kb on - strand, within Pf1N1B4_903 at 1031.081 kb on - strand, within Pf1N1B4_903 at 1031.081 kb on - strand, within Pf1N1B4_903 at 1031.081 kb on - strand, within Pf1N1B4_903 at 1031.081 kb on - strand, within Pf1N1B4_903 at 1031.201 kb on - strand, within Pf1N1B4_903 at 1031.433 kb on + strand, within Pf1N1B4_903 at 1031.434 kb on - strand, within Pf1N1B4_903 at 1031.434 kb on - strand, within Pf1N1B4_903 at 1031.434 kb on - strand, within Pf1N1B4_903 at 1031.657 kb on + strand, within Pf1N1B4_903 at 1031.657 kb on + strand, within Pf1N1B4_903 at 1031.657 kb on + strand, within Pf1N1B4_903 at 1031.657 kb on + strand, within Pf1N1B4_903 at 1031.657 kb on + strand, within Pf1N1B4_903 at 1031.780 kb on + strand at 1031.780 kb on + strand at 1031.780 kb on + strand at 1031.977 kb on + strand at 1031.978 kb on - strand at 1031.987 kb on + strand at 1031.987 kb on + strand at 1031.988 kb on - strand at 1031.988 kb on - strand at 1031.988 kb on - strand at 1031.988 kb on - strand at 1031.988 kb on - strand at 1031.988 kb on - strand at 1031.988 kb on - strand at 1031.995 kb on - strand at 1031.995 kb on - strand at 1032.033 kb on + strand, within Pf1N1B4_904 at 1032.034 kb on - strand, within Pf1N1B4_904 at 1032.223 kb on - strand, within Pf1N1B4_904 at 1032.223 kb on - strand, within Pf1N1B4_904 at 1032.255 kb on + strand at 1032.255 kb on + strand at 1032.255 kb on + strand at 1032.256 kb on - strand at 1032.258 kb on + strand at 1032.259 kb on - strand at 1032.259 kb on - strand at 1032.259 kb on - strand at 1032.259 kb on - strand at 1032.270 kb on + strand at 1032.270 kb on + strand at 1032.270 kb on + strand at 1032.270 kb on + strand at 1032.270 kb on + strand at 1032.270 kb on + strand at 1032.271 kb on - strand at 1032.271 kb on - strand at 1032.271 kb on - strand at 1032.271 kb on - strand at 1032.271 kb on - strand at 1032.271 kb on - strand at 1032.271 kb on - strand at 1032.271 kb on - strand at 1032.271 kb on - strand at 1032.296 kb on - strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.380 kb on + strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.381 kb on - strand at 1032.466 kb on + strand at 1032.466 kb on + strand at 1032.485 kb on + strand at 1032.485 kb on + strand at 1032.495 kb on + strand at 1032.495 kb on + strand at 1032.495 kb on + strand at 1032.495 kb on + strand at 1032.495 kb on + strand at 1032.495 kb on + strand at 1032.496 kb on - strand at 1032.496 kb on - strand at 1032.496 kb on - strand at 1032.496 kb on - strand at 1032.517 kb on + strand at 1032.518 kb on - strand at 1032.518 kb on - strand at 1032.526 kb on - strand at 1032.587 kb on + strand at 1032.588 kb on - strand at 1032.884 kb on + strand at 1032.884 kb on + strand, within Pf1N1B4_905 at 1032.884 kb on + strand, within Pf1N1B4_905 at 1032.884 kb on + strand, within Pf1N1B4_905 at 1032.884 kb on + strand at 1032.884 kb on + strand, within Pf1N1B4_905 at 1032.885 kb on - strand, within Pf1N1B4_905 at 1032.885 kb on - strand, within Pf1N1B4_905 at 1032.885 kb on - strand, within Pf1N1B4_905 at 1032.885 kb on - strand, within Pf1N1B4_905 at 1032.911 kb on + strand, within Pf1N1B4_905 at 1032.911 kb on + strand, within Pf1N1B4_905 at 1032.911 kb on + strand, within Pf1N1B4_905 at 1032.911 kb on + strand, within Pf1N1B4_905 at 1032.911 kb on + strand, within Pf1N1B4_905 at 1032.911 kb on + strand, within Pf1N1B4_905 at 1032.911 kb on + strand, within Pf1N1B4_905 at 1032.912 kb on - strand, within Pf1N1B4_905 at 1032.912 kb on - strand, within Pf1N1B4_905 at 1032.912 kb on - strand, within Pf1N1B4_905 at 1032.912 kb on - strand, within Pf1N1B4_905 at 1032.912 kb on - strand, within Pf1N1B4_905 at 1032.912 kb on - strand, within Pf1N1B4_905 at 1032.992 kb on + strand, within Pf1N1B4_905 at 1032.992 kb on + strand, within Pf1N1B4_905 at 1032.992 kb on + strand, within Pf1N1B4_905 at 1032.993 kb on - strand, within Pf1N1B4_905 at 1032.993 kb on - strand, within Pf1N1B4_905
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 1,031,015 - Pf1N1B4_903 0.25 -2.0 1,031,047 + Pf1N1B4_903 0.28 -1.9 1,031,048 - Pf1N1B4_903 0.29 -4.1 1,031,048 - Pf1N1B4_903 0.29 -1.6 1,031,080 + Pf1N1B4_903 0.32 +0.0 1,031,080 + Pf1N1B4_903 0.32 -2.2 1,031,081 - Pf1N1B4_903 0.32 +0.0 1,031,081 - Pf1N1B4_903 0.32 +0.0 1,031,081 - Pf1N1B4_903 0.32 +0.0 1,031,081 - Pf1N1B4_903 0.32 -0.8 1,031,081 - Pf1N1B4_903 0.32 -1.6 1,031,081 - Pf1N1B4_903 0.32 +0.0 1,031,081 - Pf1N1B4_903 0.32 +0.0 1,031,081 - Pf1N1B4_903 0.32 +0.0 1,031,081 - Pf1N1B4_903 0.32 -1.4 1,031,201 - Pf1N1B4_903 0.43 +0.1 1,031,433 + Pf1N1B4_903 0.66 +0.0 1,031,434 - Pf1N1B4_903 0.66 +0.0 1,031,434 - Pf1N1B4_903 0.66 +0.0 1,031,434 - Pf1N1B4_903 0.66 +0.0 1,031,657 + Pf1N1B4_903 0.88 -1.3 1,031,657 + Pf1N1B4_903 0.88 -4.0 1,031,657 + Pf1N1B4_903 0.88 +0.0 1,031,657 + Pf1N1B4_903 0.88 +0.0 1,031,657 + Pf1N1B4_903 0.88 +0.0 1,031,780 + -0.7 1,031,780 + +0.0 1,031,780 + -1.5 1,031,977 + -2.4 1,031,978 - +0.0 1,031,987 + +0.0 1,031,987 + +0.0 1,031,988 - -4.1 1,031,988 - -2.0 1,031,988 - -2.4 1,031,988 - -2.8 1,031,988 - +0.0 1,031,988 - +0.0 1,031,988 - +0.0 1,031,995 - +0.0 1,031,995 - +0.0 1,032,033 + Pf1N1B4_904 0.17 -4.9 1,032,034 - Pf1N1B4_904 0.17 +0.0 1,032,223 - Pf1N1B4_904 0.84 -2.6 1,032,223 - Pf1N1B4_904 0.84 +0.0 1,032,255 + +0.0 1,032,255 + +0.0 1,032,255 + -2.0 1,032,256 - +0.0 1,032,258 + +0.0 1,032,259 - -2.6 1,032,259 - +0.0 1,032,259 - +0.0 1,032,259 - -0.8 1,032,270 + +0.0 1,032,270 + +0.0 1,032,270 + -2.1 1,032,270 + +0.0 1,032,270 + -3.4 1,032,270 + +0.0 1,032,271 - -1.5 1,032,271 - -1.6 1,032,271 - -3.2 1,032,271 - +0.0 1,032,271 - -2.8 1,032,271 - +0.0 1,032,271 - +0.0 1,032,271 - -1.6 1,032,271 - +0.0 1,032,296 - +0.0 1,032,380 + +0.0 1,032,380 + +0.0 1,032,380 + +0.0 1,032,380 + +0.7 1,032,380 + -3.5 1,032,380 + -2.9 1,032,380 + +0.0 1,032,380 + -2.2 1,032,380 + -2.0 1,032,380 + +0.0 1,032,380 + -1.8 1,032,380 + -2.0 1,032,380 + -2.0 1,032,380 + +0.0 1,032,380 + -1.1 1,032,380 + +0.0 1,032,380 + +0.0 1,032,380 + -0.7 1,032,380 + -3.5 1,032,380 + +0.0 1,032,380 + +0.0 1,032,380 + +0.0 1,032,380 + +0.0 1,032,380 + +0.0 1,032,380 + -1.0 1,032,380 + -2.8 1,032,380 + +0.0 1,032,380 + +0.0 1,032,380 + -0.7 1,032,380 + -2.5 1,032,380 + +0.0 1,032,380 + +0.0 1,032,380 + +0.0 1,032,380 + +0.0 1,032,380 + -2.4 1,032,380 + +0.0 1,032,381 - +0.0 1,032,381 - +0.0 1,032,381 - +0.0 1,032,381 - -1.5 1,032,381 - +0.0 1,032,381 - -1.6 1,032,381 - -1.0 1,032,381 - -2.5 1,032,381 - -3.0 1,032,381 - +0.0 1,032,381 - +0.0 1,032,381 - +0.0 1,032,381 - -1.3 1,032,381 - -2.2 1,032,381 - -1.8 1,032,381 - -1.0 1,032,381 - +0.0 1,032,381 - +0.0 1,032,381 - +0.0 1,032,381 - +0.0 1,032,381 - +0.0 1,032,381 - +0.0 1,032,381 - -2.1 1,032,381 - +0.0 1,032,381 - +0.0 1,032,381 - +0.0 1,032,466 + +0.0 1,032,466 + -1.2 1,032,485 + +0.0 1,032,485 + +0.0 1,032,495 + +0.0 1,032,495 + +0.0 1,032,495 + +0.0 1,032,495 + +0.0 1,032,495 + +0.0 1,032,495 + +0.0 1,032,496 - -2.2 1,032,496 - -1.1 1,032,496 - -4.2 1,032,496 - -1.9 1,032,517 + -2.3 1,032,518 - +0.0 1,032,518 - +0.0 1,032,526 - +0.0 1,032,587 + -2.2 1,032,588 - +0.0 1,032,884 + -2.0 1,032,884 + Pf1N1B4_905 0.11 +0.0 1,032,884 + Pf1N1B4_905 0.11 -1.8 1,032,884 + Pf1N1B4_905 0.11 +0.0 1,032,884 + +0.0 1,032,884 + Pf1N1B4_905 0.11 -1.4 1,032,885 - Pf1N1B4_905 0.11 -3.6 1,032,885 - Pf1N1B4_905 0.11 +0.0 1,032,885 - Pf1N1B4_905 0.11 +0.0 1,032,885 - Pf1N1B4_905 0.11 +0.0 1,032,911 + Pf1N1B4_905 0.13 -2.6 1,032,911 + Pf1N1B4_905 0.13 +0.0 1,032,911 + Pf1N1B4_905 0.13 -1.8 1,032,911 + Pf1N1B4_905 0.13 +0.0 1,032,911 + Pf1N1B4_905 0.13 +0.0 1,032,911 + Pf1N1B4_905 0.13 -1.8 1,032,911 + Pf1N1B4_905 0.13 +0.0 1,032,912 - Pf1N1B4_905 0.14 +0.0 1,032,912 - Pf1N1B4_905 0.14 +0.0 1,032,912 - Pf1N1B4_905 0.14 +0.0 1,032,912 - Pf1N1B4_905 0.14 -1.5 1,032,912 - Pf1N1B4_905 0.14 -2.4 1,032,912 - Pf1N1B4_905 0.14 +0.0 1,032,992 + Pf1N1B4_905 0.21 -1.9 1,032,992 + Pf1N1B4_905 0.21 -0.7 1,032,992 + Pf1N1B4_905 0.21 -2.6 1,032,993 - Pf1N1B4_905 0.22 -3.9 1,032,993 - Pf1N1B4_905 0.22 +0.0
Or see this region's nucleotide sequence