Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_865 and Pf1N1B4_866 are separated by 50 nucleotides Pf1N1B4_866 and Pf1N1B4_867 are separated by 295 nucleotides Pf1N1B4_867 and Pf1N1B4_868 are separated by 2 nucleotides Pf1N1B4_868 and Pf1N1B4_869 are separated by 146 nucleotides
Pf1N1B4_865 - Cysteine desulfurase (EC 2.8.1.7), IscS subfamily, at 992,119 to 993,333
_865
Pf1N1B4_866 - Iron-sulfur cluster regulator IscR, at 993,384 to 993,845
_866
Pf1N1B4_867 - Serine acetyltransferase (EC 2.3.1.30), at 994,141 to 994,917
_867
Pf1N1B4_868 - tRNA:Cm32/Um32 methyltransferase, at 994,920 to 995,690
_868
Pf1N1B4_869 - Inositol-1-monophosphatase (EC 3.1.3.25), at 995,837 to 996,655
_869
Position (kb) 994
995 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 993.346 kb on - strand at 993.346 kb on - strand at 993.346 kb on - strand at 993.487 kb on - strand, within Pf1N1B4_866 at 993.487 kb on - strand, within Pf1N1B4_866 at 993.487 kb on - strand, within Pf1N1B4_866 at 993.487 kb on - strand, within Pf1N1B4_866 at 993.884 kb on - strand at 993.884 kb on - strand at 993.884 kb on - strand at 993.884 kb on - strand at 993.990 kb on - strand at 994.007 kb on + strand at 994.007 kb on + strand at 994.007 kb on + strand at 994.142 kb on + strand at 994.171 kb on + strand at 994.172 kb on - strand at 994.220 kb on + strand, within Pf1N1B4_867 at 994.221 kb on - strand, within Pf1N1B4_867 at 994.221 kb on - strand, within Pf1N1B4_867 at 994.221 kb on - strand, within Pf1N1B4_867 at 994.221 kb on - strand, within Pf1N1B4_867 at 994.273 kb on + strand, within Pf1N1B4_867 at 994.936 kb on - strand at 994.936 kb on - strand at 994.986 kb on + strand at 994.986 kb on + strand at 994.986 kb on + strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.988 kb on + strand at 994.988 kb on + strand at 994.989 kb on - strand at 995.024 kb on - strand, within Pf1N1B4_868 at 995.110 kb on + strand, within Pf1N1B4_868 at 995.111 kb on - strand at 995.111 kb on - strand, within Pf1N1B4_868 at 995.216 kb on + strand, within Pf1N1B4_868 at 995.216 kb on + strand, within Pf1N1B4_868 at 995.216 kb on + strand, within Pf1N1B4_868 at 995.216 kb on + strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.227 kb on + strand, within Pf1N1B4_868 at 995.227 kb on + strand, within Pf1N1B4_868 at 995.227 kb on + strand, within Pf1N1B4_868 at 995.227 kb on + strand, within Pf1N1B4_868 at 995.227 kb on + strand, within Pf1N1B4_868 at 995.227 kb on + strand, within Pf1N1B4_868 at 995.228 kb on - strand, within Pf1N1B4_868 at 995.228 kb on - strand, within Pf1N1B4_868 at 995.228 kb on - strand, within Pf1N1B4_868 at 995.228 kb on - strand, within Pf1N1B4_868 at 995.228 kb on - strand, within Pf1N1B4_868 at 995.299 kb on + strand, within Pf1N1B4_868 at 995.300 kb on - strand, within Pf1N1B4_868 at 995.388 kb on + strand, within Pf1N1B4_868 at 995.389 kb on - strand, within Pf1N1B4_868 at 995.730 kb on + strand at 995.742 kb on + strand at 995.742 kb on + strand at 995.753 kb on + strand at 995.753 kb on + strand at 995.753 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 993,346 - +0.0 993,346 - -1.8 993,346 - +0.0 993,487 - Pf1N1B4_866 0.22 -0.7 993,487 - Pf1N1B4_866 0.22 +0.0 993,487 - Pf1N1B4_866 0.22 -1.0 993,487 - Pf1N1B4_866 0.22 -1.0 993,884 - -4.2 993,884 - +0.0 993,884 - -3.3 993,884 - -1.2 993,990 - +0.0 994,007 + +0.0 994,007 + +0.0 994,007 + +0.0 994,142 + -0.0 994,171 + -0.0 994,172 - -1.2 994,220 + Pf1N1B4_867 0.10 -4.1 994,221 - Pf1N1B4_867 0.10 -2.4 994,221 - Pf1N1B4_867 0.10 -0.0 994,221 - Pf1N1B4_867 0.10 -0.0 994,221 - Pf1N1B4_867 0.10 -2.8 994,273 + Pf1N1B4_867 0.17 -0.0 994,936 - -0.0 994,936 - -0.0 994,986 + -0.0 994,986 + -0.0 994,986 + -1.0 994,987 - -1.0 994,987 - -0.0 994,987 - -0.0 994,987 - -0.0 994,987 - -2.9 994,987 - -3.9 994,987 - -0.0 994,987 - -0.0 994,987 - -2.9 994,987 - -0.0 994,987 - -3.1 994,987 - -0.0 994,987 - -0.0 994,988 + -0.0 994,988 + -0.9 994,989 - -0.0 995,024 - Pf1N1B4_868 0.13 -3.4 995,110 + Pf1N1B4_868 0.25 -0.0 995,111 - -3.8 995,111 - Pf1N1B4_868 0.25 -0.0 995,216 + Pf1N1B4_868 0.38 -0.0 995,216 + Pf1N1B4_868 0.38 -0.0 995,216 + Pf1N1B4_868 0.38 -0.0 995,216 + Pf1N1B4_868 0.38 -1.1 995,217 - Pf1N1B4_868 0.39 -0.0 995,217 - Pf1N1B4_868 0.39 -0.0 995,217 - Pf1N1B4_868 0.39 -0.0 995,217 - Pf1N1B4_868 0.39 -0.0 995,217 - Pf1N1B4_868 0.39 -0.0 995,217 - Pf1N1B4_868 0.39 -0.0 995,217 - Pf1N1B4_868 0.39 -0.0 995,227 + Pf1N1B4_868 0.40 -0.0 995,227 + Pf1N1B4_868 0.40 -0.0 995,227 + Pf1N1B4_868 0.40 -0.0 995,227 + Pf1N1B4_868 0.40 -2.2 995,227 + Pf1N1B4_868 0.40 -4.1 995,227 + Pf1N1B4_868 0.40 -0.0 995,228 - Pf1N1B4_868 0.40 -0.0 995,228 - Pf1N1B4_868 0.40 -0.0 995,228 - Pf1N1B4_868 0.40 -0.0 995,228 - Pf1N1B4_868 0.40 -3.2 995,228 - Pf1N1B4_868 0.40 -0.0 995,299 + Pf1N1B4_868 0.49 -0.3 995,300 - Pf1N1B4_868 0.49 -0.0 995,388 + Pf1N1B4_868 0.61 -1.5 995,389 - Pf1N1B4_868 0.61 -2.4 995,730 + -0.0 995,742 + -0.0 995,742 + -0.0 995,753 + -2.1 995,753 + -1.4 995,753 + -0.0
Or see this region's nucleotide sequence