Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_835

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_834 and Pf1N1B4_835 are separated by 92 nucleotidesPf1N1B4_835 and Pf1N1B4_836 are separated by 117 nucleotides Pf1N1B4_834 - Maltoporin (maltose/maltodextrin high-affinity receptor, phage lambda receptor protein), at 955,949 to 957,172 _834 Pf1N1B4_835 - Trehalose-6-phosphate hydrolase (EC 3.2.1.93), at 957,265 to 958,914 _835 Pf1N1B4_836 - PTS system, trehalose-specific IIB component (EC 2.7.1.69) / PTS system, trehalose-specific IIC component (EC 2.7.1.69), at 959,032 to 960,474 _836 Position (kb) 957 958 959Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 956.313 kb on + strand, within Pf1N1B4_834at 956.417 kb on - strand, within Pf1N1B4_834at 956.419 kb on - strand, within Pf1N1B4_834at 956.464 kb on + strand, within Pf1N1B4_834at 956.465 kb on - strandat 956.465 kb on - strand, within Pf1N1B4_834at 956.465 kb on - strand, within Pf1N1B4_834at 956.465 kb on - strand, within Pf1N1B4_834at 956.554 kb on - strand, within Pf1N1B4_834at 956.554 kb on - strand, within Pf1N1B4_834at 956.554 kb on - strand, within Pf1N1B4_834at 956.554 kb on - strand, within Pf1N1B4_834at 956.554 kb on - strand, within Pf1N1B4_834at 956.628 kb on + strand, within Pf1N1B4_834at 956.628 kb on + strand, within Pf1N1B4_834at 956.628 kb on + strand, within Pf1N1B4_834at 956.628 kb on + strand, within Pf1N1B4_834at 956.628 kb on + strand, within Pf1N1B4_834at 956.628 kb on + strandat 956.629 kb on - strand, within Pf1N1B4_834at 956.629 kb on - strand, within Pf1N1B4_834at 956.629 kb on - strand, within Pf1N1B4_834at 956.629 kb on - strand, within Pf1N1B4_834at 956.667 kb on + strand, within Pf1N1B4_834at 956.667 kb on + strand, within Pf1N1B4_834at 956.667 kb on + strand, within Pf1N1B4_834at 956.667 kb on + strandat 956.668 kb on - strand, within Pf1N1B4_834at 956.668 kb on - strand, within Pf1N1B4_834at 956.668 kb on - strand, within Pf1N1B4_834at 956.673 kb on + strand, within Pf1N1B4_834at 956.673 kb on + strand, within Pf1N1B4_834at 956.673 kb on + strand, within Pf1N1B4_834at 956.674 kb on - strand, within Pf1N1B4_834at 956.733 kb on + strand, within Pf1N1B4_834at 956.736 kb on + strand, within Pf1N1B4_834at 956.737 kb on - strand, within Pf1N1B4_834at 956.737 kb on - strand, within Pf1N1B4_834at 956.737 kb on - strand, within Pf1N1B4_834at 956.737 kb on - strand, within Pf1N1B4_834at 956.737 kb on - strand, within Pf1N1B4_834at 956.737 kb on - strand, within Pf1N1B4_834at 956.769 kb on + strand, within Pf1N1B4_834at 956.769 kb on + strand, within Pf1N1B4_834at 956.770 kb on - strand, within Pf1N1B4_834at 956.770 kb on - strand, within Pf1N1B4_834at 956.770 kb on - strand, within Pf1N1B4_834at 956.832 kb on + strand, within Pf1N1B4_834at 956.832 kb on + strand, within Pf1N1B4_834at 956.832 kb on + strand, within Pf1N1B4_834at 956.832 kb on + strand, within Pf1N1B4_834at 956.832 kb on + strand, within Pf1N1B4_834at 956.833 kb on - strand, within Pf1N1B4_834at 956.833 kb on - strand, within Pf1N1B4_834at 956.833 kb on - strand, within Pf1N1B4_834at 956.833 kb on - strand, within Pf1N1B4_834at 956.833 kb on - strand, within Pf1N1B4_834at 956.833 kb on - strand, within Pf1N1B4_834at 956.890 kb on - strand, within Pf1N1B4_834at 956.890 kb on - strand, within Pf1N1B4_834at 956.898 kb on + strand, within Pf1N1B4_834at 956.898 kb on + strand, within Pf1N1B4_834at 956.898 kb on + strandat 956.898 kb on + strandat 956.898 kb on + strand, within Pf1N1B4_834at 956.898 kb on + strand, within Pf1N1B4_834at 956.899 kb on - strand, within Pf1N1B4_834at 956.979 kb on + strand, within Pf1N1B4_834at 956.980 kb on - strand, within Pf1N1B4_834at 956.980 kb on - strand, within Pf1N1B4_834at 956.980 kb on - strand, within Pf1N1B4_834at 956.980 kb on - strand, within Pf1N1B4_834at 956.980 kb on - strandat 957.084 kb on + strandat 957.085 kb on - strandat 957.159 kb on - strandat 957.159 kb on - strandat 957.191 kb on + strandat 957.299 kb on + strandat 957.422 kb on + strandat 957.499 kb on + strand, within Pf1N1B4_835at 957.972 kb on + strand, within Pf1N1B4_835at 958.274 kb on + strand, within Pf1N1B4_835at 958.274 kb on + strand, within Pf1N1B4_835at 958.460 kb on + strand, within Pf1N1B4_835at 958.461 kb on - strand, within Pf1N1B4_835at 958.463 kb on + strand, within Pf1N1B4_835at 958.463 kb on + strand, within Pf1N1B4_835at 958.544 kb on + strand, within Pf1N1B4_835at 958.544 kb on + strand, within Pf1N1B4_835at 958.545 kb on - strand, within Pf1N1B4_835at 958.806 kb on - strandat 958.886 kb on + strandat 958.886 kb on + strandat 958.886 kb on + strandat 958.887 kb on - strandat 958.931 kb on + strandat 958.941 kb on - strandat 959.203 kb on - strand, within Pf1N1B4_836at 959.252 kb on + strand, within Pf1N1B4_836at 959.253 kb on - strand, within Pf1N1B4_836at 959.253 kb on - strand, within Pf1N1B4_836at 959.253 kb on - strand, within Pf1N1B4_836at 959.253 kb on - strand, within Pf1N1B4_836at 959.268 kb on - strand, within Pf1N1B4_836at 959.312 kb on + strand, within Pf1N1B4_836at 959.312 kb on + strand, within Pf1N1B4_836at 959.312 kb on + strand, within Pf1N1B4_836at 959.369 kb on + strand, within Pf1N1B4_836at 959.369 kb on + strand, within Pf1N1B4_836at 959.369 kb on + strand, within Pf1N1B4_836at 959.369 kb on + strand, within Pf1N1B4_836at 959.369 kb on + strand, within Pf1N1B4_836at 959.369 kb on + strand, within Pf1N1B4_836at 959.369 kb on + strand, within Pf1N1B4_836at 959.369 kb on + strandat 959.370 kb on - strand, within Pf1N1B4_836at 959.370 kb on - strand, within Pf1N1B4_836at 959.370 kb on - strand, within Pf1N1B4_836at 959.370 kb on - strand, within Pf1N1B4_836at 959.370 kb on - strand, within Pf1N1B4_836at 959.370 kb on - strand, within Pf1N1B4_836at 959.370 kb on - strand, within Pf1N1B4_836at 959.370 kb on - strand, within Pf1N1B4_836at 959.370 kb on - strand, within Pf1N1B4_836at 959.431 kb on - strand, within Pf1N1B4_836at 959.514 kb on - strand, within Pf1N1B4_836at 959.729 kb on + strand, within Pf1N1B4_836at 959.729 kb on + strand, within Pf1N1B4_836at 959.730 kb on - strand, within Pf1N1B4_836at 959.850 kb on - strand, within Pf1N1B4_836

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
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956,313 + Pf1N1B4_834 0.30 -1.9
956,417 - Pf1N1B4_834 0.38 -0.0
956,419 - Pf1N1B4_834 0.38 -0.3
956,464 + Pf1N1B4_834 0.42 -0.0
956,465 - -0.0
956,465 - Pf1N1B4_834 0.42 -2.3
956,465 - Pf1N1B4_834 0.42 -1.9
956,465 - Pf1N1B4_834 0.42 -1.4
956,554 - Pf1N1B4_834 0.49 -1.3
956,554 - Pf1N1B4_834 0.49 -4.0
956,554 - Pf1N1B4_834 0.49 -0.0
956,554 - Pf1N1B4_834 0.49 -0.0
956,554 - Pf1N1B4_834 0.49 -0.0
956,628 + Pf1N1B4_834 0.55 -0.0
956,628 + Pf1N1B4_834 0.55 -0.8
956,628 + Pf1N1B4_834 0.55 -0.0
956,628 + Pf1N1B4_834 0.55 -0.0
956,628 + Pf1N1B4_834 0.55 -0.0
956,628 + -1.2
956,629 - Pf1N1B4_834 0.56 -1.5
956,629 - Pf1N1B4_834 0.56 -0.0
956,629 - Pf1N1B4_834 0.56 -0.0
956,629 - Pf1N1B4_834 0.56 -0.0
956,667 + Pf1N1B4_834 0.59 -0.0
956,667 + Pf1N1B4_834 0.59 -0.0
956,667 + Pf1N1B4_834 0.59 -3.5
956,667 + -2.9
956,668 - Pf1N1B4_834 0.59 -0.0
956,668 - Pf1N1B4_834 0.59 -0.0
956,668 - Pf1N1B4_834 0.59 -1.8
956,673 + Pf1N1B4_834 0.59 -0.0
956,673 + Pf1N1B4_834 0.59 -0.0
956,673 + Pf1N1B4_834 0.59 -0.0
956,674 - Pf1N1B4_834 0.59 -0.0
956,733 + Pf1N1B4_834 0.64 -0.0
956,736 + Pf1N1B4_834 0.64 -0.0
956,737 - Pf1N1B4_834 0.64 -1.4
956,737 - Pf1N1B4_834 0.64 -0.0
956,737 - Pf1N1B4_834 0.64 -0.0
956,737 - Pf1N1B4_834 0.64 -0.0
956,737 - Pf1N1B4_834 0.64 -3.0
956,737 - Pf1N1B4_834 0.64 +0.3
956,769 + Pf1N1B4_834 0.67 -0.0
956,769 + Pf1N1B4_834 0.67 -0.0
956,770 - Pf1N1B4_834 0.67 -0.0
956,770 - Pf1N1B4_834 0.67 -2.0
956,770 - Pf1N1B4_834 0.67 -1.7
956,832 + Pf1N1B4_834 0.72 -1.9
956,832 + Pf1N1B4_834 0.72 -2.6
956,832 + Pf1N1B4_834 0.72 -2.5
956,832 + Pf1N1B4_834 0.72 -0.0
956,832 + Pf1N1B4_834 0.72 -2.9
956,833 - Pf1N1B4_834 0.72 -0.0
956,833 - Pf1N1B4_834 0.72 -0.9
956,833 - Pf1N1B4_834 0.72 -2.5
956,833 - Pf1N1B4_834 0.72 -2.3
956,833 - Pf1N1B4_834 0.72 -1.7
956,833 - Pf1N1B4_834 0.72 -0.0
956,890 - Pf1N1B4_834 0.77 -0.0
956,890 - Pf1N1B4_834 0.77 -0.0
956,898 + Pf1N1B4_834 0.78 -0.0
956,898 + Pf1N1B4_834 0.78 -0.0
956,898 + -1.2
956,898 + -0.2
956,898 + Pf1N1B4_834 0.78 -0.0
956,898 + Pf1N1B4_834 0.78 -2.2
956,899 - Pf1N1B4_834 0.78 -0.0
956,979 + Pf1N1B4_834 0.84 -0.0
956,980 - Pf1N1B4_834 0.84 -1.7
956,980 - Pf1N1B4_834 0.84 -1.9
956,980 - Pf1N1B4_834 0.84 -1.6
956,980 - Pf1N1B4_834 0.84 -2.6
956,980 - -0.0
957,084 + -0.0
957,085 - -0.0
957,159 - -0.0
957,159 - -2.3
957,191 + -1.5
957,299 + -0.0
957,422 + -1.7
957,499 + Pf1N1B4_835 0.14 -0.0
957,972 + Pf1N1B4_835 0.43 -0.0
958,274 + Pf1N1B4_835 0.61 -0.0
958,274 + Pf1N1B4_835 0.61 -0.0
958,460 + Pf1N1B4_835 0.72 -0.0
958,461 - Pf1N1B4_835 0.72 -1.0
958,463 + Pf1N1B4_835 0.73 -0.0
958,463 + Pf1N1B4_835 0.73 -0.0
958,544 + Pf1N1B4_835 0.78 -0.0
958,544 + Pf1N1B4_835 0.78 -3.9
958,545 - Pf1N1B4_835 0.78 -0.0
958,806 - -0.0
958,886 + -0.0
958,886 + -0.0
958,886 + -0.0
958,887 - -2.5
958,931 + -2.2
958,941 - -0.0
959,203 - Pf1N1B4_836 0.12 -1.9
959,252 + Pf1N1B4_836 0.15 -0.0
959,253 - Pf1N1B4_836 0.15 -0.0
959,253 - Pf1N1B4_836 0.15 -0.0
959,253 - Pf1N1B4_836 0.15 -0.0
959,253 - Pf1N1B4_836 0.15 -0.0
959,268 - Pf1N1B4_836 0.16 -1.8
959,312 + Pf1N1B4_836 0.19 -1.5
959,312 + Pf1N1B4_836 0.19 -3.1
959,312 + Pf1N1B4_836 0.19 -4.1
959,369 + Pf1N1B4_836 0.23 -0.0
959,369 + Pf1N1B4_836 0.23 -0.9
959,369 + Pf1N1B4_836 0.23 -0.0
959,369 + Pf1N1B4_836 0.23 -0.0
959,369 + Pf1N1B4_836 0.23 -2.8
959,369 + Pf1N1B4_836 0.23 -0.0
959,369 + Pf1N1B4_836 0.23 -0.0
959,369 + -0.0
959,370 - Pf1N1B4_836 0.23 -0.0
959,370 - Pf1N1B4_836 0.23 -2.6
959,370 - Pf1N1B4_836 0.23 -0.0
959,370 - Pf1N1B4_836 0.23 -0.0
959,370 - Pf1N1B4_836 0.23 -2.2
959,370 - Pf1N1B4_836 0.23 -3.2
959,370 - Pf1N1B4_836 0.23 -0.0
959,370 - Pf1N1B4_836 0.23 -1.3
959,370 - Pf1N1B4_836 0.23 -0.0
959,431 - Pf1N1B4_836 0.28 -0.0
959,514 - Pf1N1B4_836 0.33 -2.5
959,729 + Pf1N1B4_836 0.48 -0.0
959,729 + Pf1N1B4_836 0.48 -1.4
959,730 - Pf1N1B4_836 0.48 -0.9
959,850 - Pf1N1B4_836 0.57 -0.0

Or see this region's nucleotide sequence