Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_756

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_755 and Pf1N1B4_756 are separated by 175 nucleotidesPf1N1B4_756 and Pf1N1B4_757 are separated by 274 nucleotides Pf1N1B4_755 - RecA-family ATPase, at 858,442 to 859,923 _755 Pf1N1B4_756 - hypothetical protein, at 860,099 to 860,287 _756 Pf1N1B4_757 - phage-related integrase, at 860,562 to 861,812 _757 Position (kb) 860 861Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 859.237 kb on + strand, within Pf1N1B4_755at 859.237 kb on + strand, within Pf1N1B4_755at 859.392 kb on + strand, within Pf1N1B4_755at 859.392 kb on + strand, within Pf1N1B4_755at 859.392 kb on + strandat 859.392 kb on + strand, within Pf1N1B4_755at 859.392 kb on + strand, within Pf1N1B4_755at 859.392 kb on + strand, within Pf1N1B4_755at 859.392 kb on + strand, within Pf1N1B4_755at 859.393 kb on - strand, within Pf1N1B4_755at 859.393 kb on - strand, within Pf1N1B4_755at 859.393 kb on - strand, within Pf1N1B4_755at 859.393 kb on - strand, within Pf1N1B4_755at 859.393 kb on - strand, within Pf1N1B4_755at 859.393 kb on - strand, within Pf1N1B4_755at 859.396 kb on + strand, within Pf1N1B4_755at 859.396 kb on + strand, within Pf1N1B4_755at 859.396 kb on + strand, within Pf1N1B4_755at 859.396 kb on + strand, within Pf1N1B4_755at 859.397 kb on - strand, within Pf1N1B4_755at 859.397 kb on - strand, within Pf1N1B4_755at 859.397 kb on - strand, within Pf1N1B4_755at 859.397 kb on - strand, within Pf1N1B4_755at 859.450 kb on + strand, within Pf1N1B4_755at 859.450 kb on + strand, within Pf1N1B4_755at 859.450 kb on + strand, within Pf1N1B4_755at 859.450 kb on + strand, within Pf1N1B4_755at 859.451 kb on - strand, within Pf1N1B4_755at 859.451 kb on - strand, within Pf1N1B4_755at 859.451 kb on - strand, within Pf1N1B4_755at 859.634 kb on + strand, within Pf1N1B4_755at 859.634 kb on + strand, within Pf1N1B4_755at 859.634 kb on + strand, within Pf1N1B4_755at 859.635 kb on - strand, within Pf1N1B4_755at 859.635 kb on - strand, within Pf1N1B4_755at 859.635 kb on - strand, within Pf1N1B4_755at 859.635 kb on - strand, within Pf1N1B4_755at 859.635 kb on - strandat 859.635 kb on - strand, within Pf1N1B4_755at 859.658 kb on + strand, within Pf1N1B4_755at 859.658 kb on + strandat 859.658 kb on + strand, within Pf1N1B4_755at 859.658 kb on + strand, within Pf1N1B4_755at 859.658 kb on + strand, within Pf1N1B4_755at 859.658 kb on + strand, within Pf1N1B4_755at 859.658 kb on + strand, within Pf1N1B4_755at 859.658 kb on + strand, within Pf1N1B4_755at 859.658 kb on + strand, within Pf1N1B4_755at 859.659 kb on + strandat 859.659 kb on - strand, within Pf1N1B4_755at 859.659 kb on - strand, within Pf1N1B4_755at 859.659 kb on - strand, within Pf1N1B4_755at 859.659 kb on - strand, within Pf1N1B4_755at 859.659 kb on - strand, within Pf1N1B4_755at 859.659 kb on - strand, within Pf1N1B4_755at 859.662 kb on + strand, within Pf1N1B4_755at 859.673 kb on + strandat 859.673 kb on + strand, within Pf1N1B4_755at 859.673 kb on + strand, within Pf1N1B4_755at 859.673 kb on + strand, within Pf1N1B4_755at 859.673 kb on + strand, within Pf1N1B4_755at 859.674 kb on - strand, within Pf1N1B4_755at 859.674 kb on - strand, within Pf1N1B4_755at 859.674 kb on - strand, within Pf1N1B4_755at 859.674 kb on - strand, within Pf1N1B4_755at 859.674 kb on - strandat 859.674 kb on - strand, within Pf1N1B4_755at 859.687 kb on + strand, within Pf1N1B4_755at 859.739 kb on + strand, within Pf1N1B4_755at 859.745 kb on + strand, within Pf1N1B4_755at 859.745 kb on + strand, within Pf1N1B4_755at 859.745 kb on + strand, within Pf1N1B4_755at 859.745 kb on + strand, within Pf1N1B4_755at 859.746 kb on - strand, within Pf1N1B4_755at 859.746 kb on - strand, within Pf1N1B4_755at 859.746 kb on - strand, within Pf1N1B4_755at 859.814 kb on + strandat 859.815 kb on - strandat 859.815 kb on - strandat 859.903 kb on + strandat 859.903 kb on + strandat 859.904 kb on - strandat 859.904 kb on - strandat 859.923 kb on + strandat 859.923 kb on + strandat 859.923 kb on + strandat 859.923 kb on + strandat 859.923 kb on + strandat 859.923 kb on + strandat 859.923 kb on + strandat 859.923 kb on + strandat 859.925 kb on + strandat 859.925 kb on + strandat 859.925 kb on + strandat 859.925 kb on + strandat 859.925 kb on + strandat 859.925 kb on + strandat 859.925 kb on + strandat 859.925 kb on + strandat 859.926 kb on - strandat 859.926 kb on - strandat 859.926 kb on - strandat 859.926 kb on - strandat 859.959 kb on + strandat 859.960 kb on - strandat 859.961 kb on + strandat 859.962 kb on - strandat 859.963 kb on + strandat 859.963 kb on + strandat 859.963 kb on + strandat 859.963 kb on + strandat 859.963 kb on + strandat 859.964 kb on - strandat 859.964 kb on - strandat 859.964 kb on - strandat 859.964 kb on - strandat 860.053 kb on - strandat 860.053 kb on - strandat 860.100 kb on + strandat 860.101 kb on - strandat 860.101 kb on - strandat 860.101 kb on - strandat 860.101 kb on - strandat 860.180 kb on + strand, within Pf1N1B4_756at 860.181 kb on - strand, within Pf1N1B4_756at 860.181 kb on - strand, within Pf1N1B4_756at 860.181 kb on - strandat 860.183 kb on - strand, within Pf1N1B4_756at 860.185 kb on - strand, within Pf1N1B4_756at 860.372 kb on + strandat 860.558 kb on - strandat 860.569 kb on - strandat 860.708 kb on + strand, within Pf1N1B4_757at 860.709 kb on - strand, within Pf1N1B4_757at 860.719 kb on + strand, within Pf1N1B4_757at 860.720 kb on - strand, within Pf1N1B4_757at 860.777 kb on + strand, within Pf1N1B4_757at 860.784 kb on - strand, within Pf1N1B4_757at 860.784 kb on - strand, within Pf1N1B4_757at 860.835 kb on + strand, within Pf1N1B4_757at 860.835 kb on + strand, within Pf1N1B4_757at 860.835 kb on + strand, within Pf1N1B4_757at 860.835 kb on + strand, within Pf1N1B4_757at 860.835 kb on + strand, within Pf1N1B4_757at 860.835 kb on + strand, within Pf1N1B4_757at 860.835 kb on + strand, within Pf1N1B4_757at 860.836 kb on - strand, within Pf1N1B4_757at 860.836 kb on - strandat 860.836 kb on - strand, within Pf1N1B4_757at 860.836 kb on - strand, within Pf1N1B4_757at 860.976 kb on + strandat 860.976 kb on + strand, within Pf1N1B4_757at 861.037 kb on - strand, within Pf1N1B4_757at 861.037 kb on - strand, within Pf1N1B4_757at 861.037 kb on - strand, within Pf1N1B4_757at 861.037 kb on - strand, within Pf1N1B4_757at 861.049 kb on + strand, within Pf1N1B4_757at 861.049 kb on + strand, within Pf1N1B4_757at 861.049 kb on + strand, within Pf1N1B4_757at 861.050 kb on - strand, within Pf1N1B4_757at 861.050 kb on - strand, within Pf1N1B4_757at 861.050 kb on - strand, within Pf1N1B4_757at 861.079 kb on - strand, within Pf1N1B4_757at 861.188 kb on + strand, within Pf1N1B4_757at 861.189 kb on - strand, within Pf1N1B4_757at 861.284 kb on + strand, within Pf1N1B4_757at 861.284 kb on + strand, within Pf1N1B4_757at 861.285 kb on - strand, within Pf1N1B4_757at 861.285 kb on - strand, within Pf1N1B4_757at 861.285 kb on - strand, within Pf1N1B4_757at 861.285 kb on - strand, within Pf1N1B4_757at 861.285 kb on - strand, within Pf1N1B4_757at 861.285 kb on - strand, within Pf1N1B4_757at 861.285 kb on - strand, within Pf1N1B4_757

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
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859,237 + Pf1N1B4_755 0.54 +0.0
859,237 + Pf1N1B4_755 0.54 +0.0
859,392 + Pf1N1B4_755 0.64 +0.0
859,392 + Pf1N1B4_755 0.64 +0.0
859,392 + +0.0
859,392 + Pf1N1B4_755 0.64 +0.0
859,392 + Pf1N1B4_755 0.64 +0.0
859,392 + Pf1N1B4_755 0.64 +0.0
859,392 + Pf1N1B4_755 0.64 +0.0
859,393 - Pf1N1B4_755 0.64 -1.6
859,393 - Pf1N1B4_755 0.64 -0.6
859,393 - Pf1N1B4_755 0.64 -1.0
859,393 - Pf1N1B4_755 0.64 +0.0
859,393 - Pf1N1B4_755 0.64 -0.8
859,393 - Pf1N1B4_755 0.64 -1.7
859,396 + Pf1N1B4_755 0.64 +0.0
859,396 + Pf1N1B4_755 0.64 -1.0
859,396 + Pf1N1B4_755 0.64 +1.0
859,396 + Pf1N1B4_755 0.64 -2.4
859,397 - Pf1N1B4_755 0.64 -1.3
859,397 - Pf1N1B4_755 0.64 +0.0
859,397 - Pf1N1B4_755 0.64 -0.9
859,397 - Pf1N1B4_755 0.64 +0.0
859,450 + Pf1N1B4_755 0.68 +0.0
859,450 + Pf1N1B4_755 0.68 -0.6
859,450 + Pf1N1B4_755 0.68 +0.0
859,450 + Pf1N1B4_755 0.68 +0.0
859,451 - Pf1N1B4_755 0.68 +0.0
859,451 - Pf1N1B4_755 0.68 -1.9
859,451 - Pf1N1B4_755 0.68 +0.0
859,634 + Pf1N1B4_755 0.80 +0.0
859,634 + Pf1N1B4_755 0.80 +0.0
859,634 + Pf1N1B4_755 0.80 +0.0
859,635 - Pf1N1B4_755 0.80 +0.0
859,635 - Pf1N1B4_755 0.80 +0.0
859,635 - Pf1N1B4_755 0.80 -2.1
859,635 - Pf1N1B4_755 0.80 +0.0
859,635 - -1.1
859,635 - Pf1N1B4_755 0.80 -1.5
859,658 + Pf1N1B4_755 0.82 +0.0
859,658 + +0.0
859,658 + Pf1N1B4_755 0.82 +0.0
859,658 + Pf1N1B4_755 0.82 -0.2
859,658 + Pf1N1B4_755 0.82 -2.4
859,658 + Pf1N1B4_755 0.82 +0.0
859,658 + Pf1N1B4_755 0.82 +0.0
859,658 + Pf1N1B4_755 0.82 +0.0
859,658 + Pf1N1B4_755 0.82 +0.0
859,659 + +0.0
859,659 - Pf1N1B4_755 0.82 +0.0
859,659 - Pf1N1B4_755 0.82 +0.0
859,659 - Pf1N1B4_755 0.82 -0.7
859,659 - Pf1N1B4_755 0.82 +0.0
859,659 - Pf1N1B4_755 0.82 +0.0
859,659 - Pf1N1B4_755 0.82 +0.0
859,662 + Pf1N1B4_755 0.82 +0.0
859,673 + +0.0
859,673 + Pf1N1B4_755 0.83 +0.0
859,673 + Pf1N1B4_755 0.83 +0.0
859,673 + Pf1N1B4_755 0.83 +0.0
859,673 + Pf1N1B4_755 0.83 +0.0
859,674 - Pf1N1B4_755 0.83 +0.0
859,674 - Pf1N1B4_755 0.83 +0.0
859,674 - Pf1N1B4_755 0.83 +0.0
859,674 - Pf1N1B4_755 0.83 +0.0
859,674 - +0.0
859,674 - Pf1N1B4_755 0.83 +0.0
859,687 + Pf1N1B4_755 0.84 +0.0
859,739 + Pf1N1B4_755 0.88 +0.0
859,745 + Pf1N1B4_755 0.88 +0.0
859,745 + Pf1N1B4_755 0.88 +0.0
859,745 + Pf1N1B4_755 0.88 +0.0
859,745 + Pf1N1B4_755 0.88 +0.0
859,746 - Pf1N1B4_755 0.88 -1.4
859,746 - Pf1N1B4_755 0.88 +0.0
859,746 - Pf1N1B4_755 0.88 +0.0
859,814 + +0.0
859,815 - +0.0
859,815 - -1.9
859,903 + -0.9
859,903 + -0.8
859,904 - +0.0
859,904 - -1.4
859,923 + +0.0
859,923 + -3.6
859,923 + +0.0
859,923 + +0.0
859,923 + -2.5
859,923 + +0.0
859,923 + -3.1
859,923 + -1.2
859,925 + -1.0
859,925 + +0.0
859,925 + -3.0
859,925 + +0.0
859,925 + +0.0
859,925 + -0.9
859,925 + +0.0
859,925 + +0.0
859,926 - +0.0
859,926 - +0.0
859,926 - +0.0
859,926 - +0.0
859,959 + +0.0
859,960 - -1.6
859,961 + -0.6
859,962 - -2.7
859,963 + +0.0
859,963 + +0.0
859,963 + +0.0
859,963 + +0.0
859,963 + -1.2
859,964 - +0.0
859,964 - +0.0
859,964 - -1.7
859,964 - +0.0
860,053 - -1.6
860,053 - +0.0
860,100 + +0.0
860,101 - -1.8
860,101 - -1.2
860,101 - -1.6
860,101 - +0.0
860,180 + Pf1N1B4_756 0.43 +0.0
860,181 - Pf1N1B4_756 0.43 +0.0
860,181 - Pf1N1B4_756 0.43 +0.0
860,181 - +0.0
860,183 - Pf1N1B4_756 0.44 +0.0
860,185 - Pf1N1B4_756 0.46 -3.4
860,372 + -1.0
860,558 - -2.3
860,569 - +0.0
860,708 + Pf1N1B4_757 0.12 +0.0
860,709 - Pf1N1B4_757 0.12 -1.6
860,719 + Pf1N1B4_757 0.13 +0.0
860,720 - Pf1N1B4_757 0.13 -2.9
860,777 + Pf1N1B4_757 0.17 -1.3
860,784 - Pf1N1B4_757 0.18 -2.1
860,784 - Pf1N1B4_757 0.18 +0.0
860,835 + Pf1N1B4_757 0.22 +0.0
860,835 + Pf1N1B4_757 0.22 -1.0
860,835 + Pf1N1B4_757 0.22 +0.0
860,835 + Pf1N1B4_757 0.22 +0.0
860,835 + Pf1N1B4_757 0.22 +0.0
860,835 + Pf1N1B4_757 0.22 +0.0
860,835 + Pf1N1B4_757 0.22 -1.4
860,836 - Pf1N1B4_757 0.22 -1.9
860,836 - -0.7
860,836 - Pf1N1B4_757 0.22 +0.0
860,836 - Pf1N1B4_757 0.22 +0.0
860,976 + +0.0
860,976 + Pf1N1B4_757 0.33 +1.0
861,037 - Pf1N1B4_757 0.38 -1.7
861,037 - Pf1N1B4_757 0.38 +0.0
861,037 - Pf1N1B4_757 0.38 +0.0
861,037 - Pf1N1B4_757 0.38 +0.0
861,049 + Pf1N1B4_757 0.39 +0.0
861,049 + Pf1N1B4_757 0.39 +0.0
861,049 + Pf1N1B4_757 0.39 +0.0
861,050 - Pf1N1B4_757 0.39 -1.6
861,050 - Pf1N1B4_757 0.39 -2.6
861,050 - Pf1N1B4_757 0.39 +0.0
861,079 - Pf1N1B4_757 0.41 -0.8
861,188 + Pf1N1B4_757 0.50 -2.5
861,189 - Pf1N1B4_757 0.50 +0.0
861,284 + Pf1N1B4_757 0.58 -4.6
861,284 + Pf1N1B4_757 0.58 +0.0
861,285 - Pf1N1B4_757 0.58 -0.4
861,285 - Pf1N1B4_757 0.58 -0.9
861,285 - Pf1N1B4_757 0.58 +0.0
861,285 - Pf1N1B4_757 0.58 +0.0
861,285 - Pf1N1B4_757 0.58 -2.0
861,285 - Pf1N1B4_757 0.58 +0.0
861,285 - Pf1N1B4_757 0.58 +0.0

Or see this region's nucleotide sequence