Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_607 and Pf1N1B4_608 are separated by 109 nucleotides Pf1N1B4_608 and Pf1N1B4_609 are separated by 164 nucleotides Pf1N1B4_609 and Pf1N1B4_610 are separated by 177 nucleotides
Pf1N1B4_607 - TonB-dependent hemin , ferrichrome receptor, at 699,251 to 701,812
_607
Pf1N1B4_608 - Heme oxygenase HemO, associated with heme uptake, at 701,922 to 702,530
_608
Pf1N1B4_609 - FIG039061: hypothetical protein related to heme utilization, at 702,695 to 703,090
_609
Pf1N1B4_610 - Methyl-accepting chemotaxis transducer, at 703,268 to 705,406
_610
Position (kb) 701
702
703 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 701.012 kb on + strand, within Pf1N1B4_607 at 701.013 kb on - strand, within Pf1N1B4_607 at 701.013 kb on - strand, within Pf1N1B4_607 at 701.013 kb on - strand, within Pf1N1B4_607 at 701.013 kb on - strand, within Pf1N1B4_607 at 701.030 kb on + strand, within Pf1N1B4_607 at 701.031 kb on - strand, within Pf1N1B4_607 at 701.031 kb on - strand, within Pf1N1B4_607 at 701.031 kb on - strand, within Pf1N1B4_607 at 701.087 kb on + strand, within Pf1N1B4_607 at 701.087 kb on + strand, within Pf1N1B4_607 at 701.087 kb on + strand, within Pf1N1B4_607 at 701.087 kb on + strand, within Pf1N1B4_607 at 701.087 kb on + strand, within Pf1N1B4_607 at 701.087 kb on + strand, within Pf1N1B4_607 at 701.087 kb on + strand, within Pf1N1B4_607 at 701.088 kb on - strand at 701.088 kb on - strand, within Pf1N1B4_607 at 701.088 kb on - strand, within Pf1N1B4_607 at 701.088 kb on - strand, within Pf1N1B4_607 at 701.088 kb on - strand, within Pf1N1B4_607 at 701.088 kb on - strand, within Pf1N1B4_607 at 701.165 kb on + strand, within Pf1N1B4_607 at 701.166 kb on - strand, within Pf1N1B4_607 at 701.166 kb on - strand, within Pf1N1B4_607 at 701.166 kb on - strand, within Pf1N1B4_607 at 701.222 kb on + strand, within Pf1N1B4_607 at 701.223 kb on - strand at 701.223 kb on - strand, within Pf1N1B4_607 at 701.223 kb on - strand, within Pf1N1B4_607 at 701.223 kb on - strand, within Pf1N1B4_607 at 701.223 kb on - strand, within Pf1N1B4_607 at 701.223 kb on - strand at 701.232 kb on - strand, within Pf1N1B4_607 at 701.378 kb on + strand, within Pf1N1B4_607 at 701.378 kb on + strand, within Pf1N1B4_607 at 701.378 kb on + strand, within Pf1N1B4_607 at 701.378 kb on + strand, within Pf1N1B4_607 at 701.378 kb on + strand, within Pf1N1B4_607 at 701.378 kb on + strand, within Pf1N1B4_607 at 701.379 kb on - strand, within Pf1N1B4_607 at 701.381 kb on - strand, within Pf1N1B4_607 at 701.400 kb on - strand, within Pf1N1B4_607 at 701.400 kb on - strand, within Pf1N1B4_607 at 701.477 kb on + strand, within Pf1N1B4_607 at 701.492 kb on + strand, within Pf1N1B4_607 at 701.492 kb on + strand, within Pf1N1B4_607 at 701.492 kb on + strand, within Pf1N1B4_607 at 701.493 kb on - strand, within Pf1N1B4_607 at 701.493 kb on - strand, within Pf1N1B4_607 at 701.624 kb on + strand at 701.624 kb on + strand at 701.624 kb on + strand at 701.624 kb on + strand at 701.625 kb on - strand at 701.663 kb on + strand at 701.663 kb on + strand at 701.663 kb on + strand at 701.664 kb on - strand at 701.664 kb on - strand at 701.664 kb on - strand at 701.664 kb on - strand at 701.684 kb on + strand at 701.684 kb on + strand at 701.685 kb on - strand at 701.685 kb on - strand at 702.075 kb on + strand, within Pf1N1B4_608 at 702.076 kb on - strand, within Pf1N1B4_608 at 702.076 kb on - strand, within Pf1N1B4_608 at 702.230 kb on + strand, within Pf1N1B4_608 at 702.487 kb on + strand at 702.487 kb on + strand at 702.488 kb on - strand at 702.504 kb on + strand at 702.604 kb on + strand at 702.682 kb on - strand at 702.741 kb on + strand, within Pf1N1B4_609 at 702.766 kb on - strand, within Pf1N1B4_609 at 702.766 kb on - strand, within Pf1N1B4_609 at 702.766 kb on - strand, within Pf1N1B4_609 at 702.766 kb on - strand, within Pf1N1B4_609 at 702.766 kb on - strand at 702.766 kb on - strand, within Pf1N1B4_609 at 702.766 kb on - strand, within Pf1N1B4_609 at 703.160 kb on + strand at 703.160 kb on + strand at 703.160 kb on + strand at 703.160 kb on + strand at 703.161 kb on - strand at 703.267 kb on + strand at 703.267 kb on + strand at 703.268 kb on - strand at 703.361 kb on + strand at 703.361 kb on + strand at 703.361 kb on + strand at 703.361 kb on + strand at 703.362 kb on - strand at 703.362 kb on - strand at 703.362 kb on - strand at 703.418 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 701,012 + Pf1N1B4_607 0.69 +0.0 701,013 - Pf1N1B4_607 0.69 -0.9 701,013 - Pf1N1B4_607 0.69 +0.0 701,013 - Pf1N1B4_607 0.69 +0.0 701,013 - Pf1N1B4_607 0.69 +0.0 701,030 + Pf1N1B4_607 0.69 +0.0 701,031 - Pf1N1B4_607 0.69 -1.3 701,031 - Pf1N1B4_607 0.69 +0.0 701,031 - Pf1N1B4_607 0.69 +0.0 701,087 + Pf1N1B4_607 0.72 +0.0 701,087 + Pf1N1B4_607 0.72 -1.6 701,087 + Pf1N1B4_607 0.72 -2.0 701,087 + Pf1N1B4_607 0.72 -1.5 701,087 + Pf1N1B4_607 0.72 -0.9 701,087 + Pf1N1B4_607 0.72 +0.0 701,087 + Pf1N1B4_607 0.72 -3.7 701,088 - +0.0 701,088 - Pf1N1B4_607 0.72 +0.0 701,088 - Pf1N1B4_607 0.72 +0.0 701,088 - Pf1N1B4_607 0.72 +0.0 701,088 - Pf1N1B4_607 0.72 +0.0 701,088 - Pf1N1B4_607 0.72 -1.3 701,165 + Pf1N1B4_607 0.75 +0.0 701,166 - Pf1N1B4_607 0.75 -3.4 701,166 - Pf1N1B4_607 0.75 -1.0 701,166 - Pf1N1B4_607 0.75 -2.7 701,222 + Pf1N1B4_607 0.77 +0.0 701,223 - +0.0 701,223 - Pf1N1B4_607 0.77 -1.9 701,223 - Pf1N1B4_607 0.77 +0.0 701,223 - Pf1N1B4_607 0.77 -1.9 701,223 - Pf1N1B4_607 0.77 -2.4 701,223 - +0.0 701,232 - Pf1N1B4_607 0.77 +0.0 701,378 + Pf1N1B4_607 0.83 +0.0 701,378 + Pf1N1B4_607 0.83 +0.0 701,378 + Pf1N1B4_607 0.83 +0.0 701,378 + Pf1N1B4_607 0.83 -1.8 701,378 + Pf1N1B4_607 0.83 +0.0 701,378 + Pf1N1B4_607 0.83 -1.2 701,379 - Pf1N1B4_607 0.83 +0.0 701,381 - Pf1N1B4_607 0.83 +0.0 701,400 - Pf1N1B4_607 0.84 +0.0 701,400 - Pf1N1B4_607 0.84 +0.0 701,477 + Pf1N1B4_607 0.87 +0.0 701,492 + Pf1N1B4_607 0.87 -1.7 701,492 + Pf1N1B4_607 0.87 -1.3 701,492 + Pf1N1B4_607 0.87 +0.0 701,493 - Pf1N1B4_607 0.88 -1.8 701,493 - Pf1N1B4_607 0.88 -1.9 701,624 + -1.3 701,624 + +0.0 701,624 + -1.4 701,624 + +0.0 701,625 - +0.0 701,663 + -1.2 701,663 + -1.1 701,663 + +0.0 701,664 - +0.0 701,664 - +0.0 701,664 - +0.0 701,664 - -1.5 701,684 + +0.0 701,684 + +0.0 701,685 - +0.0 701,685 - -2.0 702,075 + Pf1N1B4_608 0.25 -1.0 702,076 - Pf1N1B4_608 0.25 +0.0 702,076 - Pf1N1B4_608 0.25 +0.0 702,230 + Pf1N1B4_608 0.51 -3.5 702,487 + +0.0 702,487 + +0.0 702,488 - +0.0 702,504 + +0.0 702,604 + -1.6 702,682 - -1.5 702,741 + Pf1N1B4_609 0.12 +0.0 702,766 - Pf1N1B4_609 0.18 -1.4 702,766 - Pf1N1B4_609 0.18 -1.6 702,766 - Pf1N1B4_609 0.18 -3.6 702,766 - Pf1N1B4_609 0.18 +1.0 702,766 - +0.0 702,766 - Pf1N1B4_609 0.18 +0.0 702,766 - Pf1N1B4_609 0.18 +0.0 703,160 + +0.0 703,160 + -3.2 703,160 + +0.0 703,160 + +0.0 703,161 - +0.0 703,267 + +0.0 703,267 + +0.0 703,268 - -1.0 703,361 + -1.9 703,361 + +0.0 703,361 + -1.2 703,361 + +0.0 703,362 - +0.0 703,362 - +0.0 703,362 - +0.0 703,418 - +0.0
Or see this region's nucleotide sequence