Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_6049 and Pf1N1B4_6050 overlap by 4 nucleotides Pf1N1B4_6050 and Pf1N1B4_6056 are separated by 277 nucleotides Pf1N1B4_6056 and Pf1N1B4_6057 are separated by 96 nucleotides Pf1N1B4_6057 and Pf1N1B4_6058 are separated by 58 nucleotides Pf1N1B4_6058 and Pf1N1B4_6051 are separated by 247 nucleotides
Pf1N1B4_6049 - Fe2+-dicitrate sensor, membrane component, at 516,786 to 517,730
_6049
Pf1N1B4_6050 - RNA polymerase sigma-70 factor, ECF subfamily, at 517,727 to 518,221
_6050
Pf1N1B4_6056 - tRNA-Asp-GTC, at 518,499 to 518,572
_6056
Pf1N1B4_6057 - tRNA-Asp-GTC, at 518,669 to 518,742
_6057
Pf1N1B4_6058 - tRNA-Val-TAC, at 518,801 to 518,873
_6058
Pf1N1B4_6051 - Oxidoreductase, short chain dehydrogenase/reductase family, at 519,121 to 519,552
_6051
Position (kb) 517
518
519 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 516.794 kb on + strand at 517.395 kb on - strand, within Pf1N1B4_6049 at 517.491 kb on + strand, within Pf1N1B4_6049 at 517.492 kb on - strand, within Pf1N1B4_6049 at 517.756 kb on - strand at 517.770 kb on + strand at 517.770 kb on + strand at 517.770 kb on + strand at 517.771 kb on - strand at 517.771 kb on - strand at 517.822 kb on - strand, within Pf1N1B4_6050 at 517.858 kb on + strand at 517.859 kb on - strand, within Pf1N1B4_6050 at 517.862 kb on + strand at 517.980 kb on + strand, within Pf1N1B4_6050 at 517.981 kb on - strand, within Pf1N1B4_6050 at 518.008 kb on - strand, within Pf1N1B4_6050 at 518.046 kb on + strand at 518.046 kb on + strand, within Pf1N1B4_6050 at 518.108 kb on - strand, within Pf1N1B4_6050 at 518.316 kb on - strand at 518.495 kb on - strand at 518.599 kb on - strand at 519.045 kb on - strand at 519.114 kb on - strand at 519.132 kb on - strand at 519.132 kb on - strand at 519.132 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 516,794 + -1.2 517,395 - Pf1N1B4_6049 0.64 -0.0 517,491 + Pf1N1B4_6049 0.75 -2.5 517,492 - Pf1N1B4_6049 0.75 -0.0 517,756 - -0.0 517,770 + -3.4 517,770 + -0.0 517,770 + -0.0 517,771 - -0.0 517,771 - -0.0 517,822 - Pf1N1B4_6050 0.19 -0.0 517,858 + -2.2 517,859 - Pf1N1B4_6050 0.27 -0.0 517,862 + -0.0 517,980 + Pf1N1B4_6050 0.51 -0.0 517,981 - Pf1N1B4_6050 0.51 -0.9 518,008 - Pf1N1B4_6050 0.57 -2.0 518,046 + -1.0 518,046 + Pf1N1B4_6050 0.64 -0.2 518,108 - Pf1N1B4_6050 0.77 -0.5 518,316 - -1.8 518,495 - -2.6 518,599 - -0.0 519,045 - -0.0 519,114 - -0.0 519,132 - -0.0 519,132 - -1.8 519,132 - -0.0
Or see this region's nucleotide sequence