Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5924 and Pf1N1B4_5925 are separated by 53 nucleotides Pf1N1B4_5925 and Pf1N1B4_5926 are separated by 38 nucleotides
Pf1N1B4_5924 - hypothetical protein, at 369,220 to 369,531
_5924
Pf1N1B4_5925 - hypothetical protein, at 369,585 to 370,298
_5925
Pf1N1B4_5926 - hypothetical protein, at 370,337 to 371,005
_5926
Position (kb) 369
370
371 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 368.586 kb on - strand at 368.586 kb on - strand at 368.586 kb on - strand at 368.586 kb on - strand at 368.595 kb on - strand at 368.622 kb on - strand at 368.622 kb on - strand at 368.622 kb on - strand at 368.622 kb on - strand at 368.622 kb on - strand at 368.649 kb on + strand at 368.650 kb on - strand at 368.650 kb on - strand at 368.650 kb on - strand at 368.668 kb on - strand at 368.668 kb on - strand at 368.668 kb on - strand at 368.668 kb on - strand at 368.691 kb on - strand at 368.691 kb on - strand at 368.731 kb on - strand at 368.733 kb on - strand at 368.766 kb on - strand at 368.766 kb on - strand at 368.786 kb on + strand at 368.787 kb on - strand at 368.787 kb on - strand at 368.787 kb on - strand at 368.787 kb on - strand at 368.787 kb on - strand at 368.787 kb on - strand at 368.792 kb on + strand at 368.792 kb on + strand at 368.793 kb on - strand at 368.793 kb on - strand at 368.808 kb on + strand at 368.842 kb on - strand at 368.891 kb on + strand at 368.891 kb on + strand at 368.891 kb on + strand at 368.892 kb on - strand at 368.892 kb on - strand at 368.897 kb on + strand at 368.901 kb on + strand at 368.917 kb on + strand at 368.919 kb on + strand at 368.919 kb on + strand at 368.920 kb on - strand at 368.920 kb on - strand at 368.924 kb on + strand at 368.924 kb on + strand at 368.924 kb on + strand at 368.924 kb on + strand at 368.925 kb on - strand at 368.925 kb on - strand at 368.925 kb on - strand at 369.005 kb on + strand at 369.005 kb on + strand at 369.005 kb on + strand at 369.006 kb on - strand at 369.006 kb on - strand at 369.009 kb on + strand at 369.010 kb on - strand at 369.039 kb on - strand at 369.039 kb on - strand at 369.042 kb on + strand at 369.067 kb on - strand at 369.082 kb on + strand at 369.082 kb on + strand at 369.112 kb on + strand at 369.112 kb on + strand at 369.112 kb on + strand at 369.143 kb on + strand at 369.143 kb on + strand at 369.143 kb on + strand at 369.143 kb on + strand at 369.143 kb on + strand at 369.143 kb on + strand at 369.144 kb on - strand at 369.144 kb on - strand at 369.147 kb on + strand at 369.214 kb on - strand at 369.215 kb on + strand at 369.215 kb on + strand at 369.216 kb on - strand at 369.248 kb on + strand at 369.248 kb on + strand at 369.248 kb on + strand at 369.248 kb on + strand at 369.249 kb on - strand at 369.249 kb on - strand at 369.249 kb on - strand at 369.249 kb on - strand at 369.249 kb on - strand at 369.250 kb on + strand at 369.250 kb on + strand at 369.250 kb on + strand at 369.251 kb on - strand at 369.251 kb on - strand at 369.251 kb on - strand at 369.251 kb on - strand at 369.328 kb on - strand, within Pf1N1B4_5924 at 369.369 kb on + strand, within Pf1N1B4_5924 at 369.369 kb on + strand, within Pf1N1B4_5924 at 369.369 kb on + strand, within Pf1N1B4_5924 at 369.370 kb on - strand, within Pf1N1B4_5924 at 369.371 kb on + strand, within Pf1N1B4_5924 at 369.371 kb on + strand, within Pf1N1B4_5924 at 369.371 kb on + strand, within Pf1N1B4_5924 at 369.371 kb on + strand, within Pf1N1B4_5924 at 369.372 kb on - strand, within Pf1N1B4_5924 at 369.379 kb on + strand, within Pf1N1B4_5924 at 369.407 kb on + strand, within Pf1N1B4_5924 at 369.423 kb on + strand, within Pf1N1B4_5924 at 369.464 kb on + strand, within Pf1N1B4_5924 at 369.464 kb on + strand, within Pf1N1B4_5924 at 369.464 kb on + strand, within Pf1N1B4_5924 at 369.465 kb on - strand, within Pf1N1B4_5924 at 369.465 kb on - strand, within Pf1N1B4_5924 at 369.465 kb on - strand, within Pf1N1B4_5924 at 369.465 kb on - strand, within Pf1N1B4_5924 at 369.529 kb on + strand at 369.529 kb on + strand at 369.529 kb on + strand at 369.529 kb on + strand at 369.530 kb on - strand at 369.563 kb on + strand at 369.563 kb on + strand at 369.564 kb on - strand at 369.564 kb on - strand at 369.564 kb on - strand at 369.564 kb on - strand at 369.564 kb on - strand at 369.564 kb on - strand at 369.564 kb on - strand at 369.564 kb on - strand at 369.564 kb on - strand at 369.590 kb on - strand at 369.645 kb on + strand at 369.646 kb on - strand at 369.723 kb on + strand, within Pf1N1B4_5925 at 369.723 kb on + strand, within Pf1N1B4_5925 at 369.724 kb on - strand, within Pf1N1B4_5925 at 369.724 kb on - strand, within Pf1N1B4_5925 at 369.724 kb on - strand, within Pf1N1B4_5925 at 369.724 kb on - strand, within Pf1N1B4_5925 at 369.724 kb on - strand, within Pf1N1B4_5925 at 369.724 kb on - strand, within Pf1N1B4_5925 at 369.724 kb on - strand, within Pf1N1B4_5925 at 369.724 kb on - strand at 369.726 kb on + strand, within Pf1N1B4_5925 at 369.727 kb on - strand, within Pf1N1B4_5925 at 369.727 kb on - strand, within Pf1N1B4_5925 at 369.727 kb on - strand, within Pf1N1B4_5925 at 369.908 kb on - strand, within Pf1N1B4_5925 at 369.921 kb on - strand, within Pf1N1B4_5925 at 369.921 kb on - strand, within Pf1N1B4_5925 at 369.921 kb on - strand, within Pf1N1B4_5925 at 369.921 kb on - strand, within Pf1N1B4_5925 at 369.966 kb on - strand, within Pf1N1B4_5925 at 369.978 kb on - strand, within Pf1N1B4_5925 at 369.986 kb on - strand, within Pf1N1B4_5925 at 370.024 kb on - strand, within Pf1N1B4_5925 at 370.030 kb on + strand, within Pf1N1B4_5925 at 370.031 kb on - strand, within Pf1N1B4_5925 at 370.031 kb on - strand, within Pf1N1B4_5925 at 370.031 kb on - strand, within Pf1N1B4_5925 at 370.031 kb on - strand, within Pf1N1B4_5925 at 370.078 kb on - strand, within Pf1N1B4_5925 at 370.082 kb on - strand at 370.082 kb on - strand, within Pf1N1B4_5925 at 370.082 kb on - strand, within Pf1N1B4_5925 at 370.082 kb on - strand, within Pf1N1B4_5925 at 370.082 kb on - strand, within Pf1N1B4_5925 at 370.082 kb on - strand, within Pf1N1B4_5925 at 370.082 kb on - strand, within Pf1N1B4_5925 at 370.082 kb on - strand, within Pf1N1B4_5925 at 370.153 kb on - strand, within Pf1N1B4_5925 at 370.153 kb on - strand, within Pf1N1B4_5925 at 370.153 kb on - strand, within Pf1N1B4_5925 at 370.153 kb on - strand, within Pf1N1B4_5925 at 370.153 kb on - strand, within Pf1N1B4_5925 at 370.272 kb on + strand at 370.272 kb on + strand at 370.273 kb on - strand at 370.273 kb on - strand at 370.273 kb on - strand at 370.275 kb on + strand at 370.276 kb on - strand at 370.276 kb on - strand at 370.276 kb on - strand at 370.292 kb on + strand at 370.293 kb on - strand at 370.321 kb on - strand at 370.321 kb on - strand at 370.333 kb on + strand at 370.334 kb on - strand at 370.424 kb on + strand, within Pf1N1B4_5926 at 370.424 kb on + strand, within Pf1N1B4_5926 at 370.424 kb on + strand, within Pf1N1B4_5926 at 370.424 kb on + strand, within Pf1N1B4_5926 at 370.424 kb on + strand, within Pf1N1B4_5926 at 370.425 kb on - strand, within Pf1N1B4_5926 at 370.428 kb on + strand, within Pf1N1B4_5926 at 370.428 kb on + strand, within Pf1N1B4_5926 at 370.429 kb on - strand, within Pf1N1B4_5926 at 370.429 kb on - strand, within Pf1N1B4_5926 at 370.515 kb on + strand, within Pf1N1B4_5926 at 370.516 kb on - strand, within Pf1N1B4_5926 at 370.567 kb on + strand, within Pf1N1B4_5926 at 370.575 kb on + strand, within Pf1N1B4_5926 at 370.576 kb on - strand, within Pf1N1B4_5926 at 370.576 kb on - strand, within Pf1N1B4_5926 at 370.576 kb on - strand, within Pf1N1B4_5926 at 370.576 kb on - strand, within Pf1N1B4_5926 at 370.711 kb on + strand, within Pf1N1B4_5926 at 370.711 kb on + strand, within Pf1N1B4_5926 at 370.711 kb on + strand, within Pf1N1B4_5926 at 370.712 kb on - strand, within Pf1N1B4_5926 at 370.712 kb on - strand, within Pf1N1B4_5926 at 370.762 kb on + strand, within Pf1N1B4_5926 at 370.762 kb on + strand, within Pf1N1B4_5926 at 370.762 kb on + strand, within Pf1N1B4_5926 at 370.763 kb on - strand, within Pf1N1B4_5926 at 370.825 kb on - strand, within Pf1N1B4_5926 at 370.825 kb on - strand, within Pf1N1B4_5926 at 370.825 kb on - strand, within Pf1N1B4_5926 at 370.843 kb on + strand, within Pf1N1B4_5926 at 370.843 kb on + strand, within Pf1N1B4_5926 at 370.844 kb on - strand, within Pf1N1B4_5926 at 370.844 kb on - strand, within Pf1N1B4_5926 at 370.844 kb on - strand, within Pf1N1B4_5926 at 370.848 kb on + strand, within Pf1N1B4_5926 at 370.875 kb on - strand, within Pf1N1B4_5926 at 370.875 kb on - strand, within Pf1N1B4_5926 at 370.876 kb on + strand, within Pf1N1B4_5926 at 370.876 kb on + strand, within Pf1N1B4_5926 at 370.877 kb on - strand, within Pf1N1B4_5926 at 370.877 kb on - strand, within Pf1N1B4_5926 at 370.989 kb on + strand at 370.989 kb on + strand at 370.989 kb on + strand at 370.990 kb on - strand at 370.990 kb on - strand at 371.059 kb on - strand at 371.125 kb on - strand at 371.247 kb on - strand at 371.247 kb on - strand at 371.247 kb on - strand at 371.247 kb on - strand at 371.263 kb on + strand at 371.290 kb on - strand at 371.296 kb on + strand at 371.296 kb on + strand at 371.296 kb on + strand at 371.297 kb on - strand at 371.297 kb on - strand at 371.297 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 368,586 - -2.9 368,586 - -3.0 368,586 - -2.5 368,586 - -0.0 368,595 - -1.7 368,622 - -0.0 368,622 - -2.8 368,622 - -3.4 368,622 - -2.2 368,622 - -2.3 368,649 + -2.4 368,650 - -2.0 368,650 - -2.2 368,650 - -0.0 368,668 - -2.7 368,668 - -0.0 368,668 - -2.2 368,668 - -2.6 368,691 - -2.4 368,691 - -2.7 368,731 - -2.7 368,733 - -2.2 368,766 - -2.4 368,766 - -4.7 368,786 + -0.0 368,787 - -0.0 368,787 - -3.2 368,787 - -4.1 368,787 - -2.5 368,787 - -0.0 368,787 - -3.6 368,792 + -0.0 368,792 + -0.0 368,793 - -2.6 368,793 - -3.7 368,808 + -3.1 368,842 - -3.0 368,891 + -0.0 368,891 + -0.0 368,891 + -0.0 368,892 - -2.3 368,892 - -3.4 368,897 + -3.1 368,901 + -2.1 368,917 + -1.8 368,919 + -2.5 368,919 + -0.0 368,920 - -0.0 368,920 - -2.3 368,924 + -2.6 368,924 + -2.3 368,924 + -0.0 368,924 + -2.7 368,925 - -4.0 368,925 - -3.5 368,925 - -3.8 369,005 + -0.0 369,005 + +0.2 369,005 + +0.8 369,006 - -4.5 369,006 - -2.1 369,009 + -3.2 369,010 - -2.3 369,039 - -0.0 369,039 - -2.2 369,042 + -2.5 369,067 - -0.0 369,082 + -2.5 369,082 + -3.1 369,112 + -2.4 369,112 + -0.5 369,112 + -0.0 369,143 + -0.0 369,143 + -2.7 369,143 + -2.6 369,143 + -3.7 369,143 + -2.4 369,143 + -3.7 369,144 - -0.0 369,144 - -0.0 369,147 + -3.7 369,214 - -2.8 369,215 + -2.9 369,215 + -2.6 369,216 - -0.0 369,248 + -2.4 369,248 + -1.5 369,248 + -3.8 369,248 + -0.0 369,249 - -2.1 369,249 - -0.0 369,249 - -0.0 369,249 - +1.0 369,249 - -0.7 369,250 + -0.0 369,250 + -0.0 369,250 + -2.9 369,251 - -3.3 369,251 - -2.6 369,251 - -1.4 369,251 - -1.0 369,328 - Pf1N1B4_5924 0.35 -2.0 369,369 + Pf1N1B4_5924 0.48 -2.8 369,369 + Pf1N1B4_5924 0.48 -0.0 369,369 + Pf1N1B4_5924 0.48 -3.6 369,370 - Pf1N1B4_5924 0.48 -4.0 369,371 + Pf1N1B4_5924 0.48 -2.8 369,371 + Pf1N1B4_5924 0.48 -0.0 369,371 + Pf1N1B4_5924 0.48 -0.0 369,371 + Pf1N1B4_5924 0.48 -2.5 369,372 - Pf1N1B4_5924 0.49 -0.0 369,379 + Pf1N1B4_5924 0.51 -2.9 369,407 + Pf1N1B4_5924 0.60 -2.8 369,423 + Pf1N1B4_5924 0.65 -2.7 369,464 + Pf1N1B4_5924 0.78 -0.0 369,464 + Pf1N1B4_5924 0.78 -2.9 369,464 + Pf1N1B4_5924 0.78 -3.7 369,465 - Pf1N1B4_5924 0.79 -4.8 369,465 - Pf1N1B4_5924 0.79 -1.0 369,465 - Pf1N1B4_5924 0.79 -0.0 369,465 - Pf1N1B4_5924 0.79 -1.6 369,529 + -0.0 369,529 + -0.9 369,529 + -1.4 369,529 + -0.5 369,530 - -2.2 369,563 + -3.1 369,563 + -3.2 369,564 - -2.5 369,564 - -0.0 369,564 - -1.9 369,564 - -0.0 369,564 - -0.0 369,564 - -1.3 369,564 - -3.9 369,564 - -0.0 369,564 - -2.2 369,590 - -2.5 369,645 + -2.7 369,646 - -3.2 369,723 + Pf1N1B4_5925 0.19 -2.3 369,723 + Pf1N1B4_5925 0.19 -3.7 369,724 - Pf1N1B4_5925 0.19 -2.8 369,724 - Pf1N1B4_5925 0.19 -2.7 369,724 - Pf1N1B4_5925 0.19 -0.0 369,724 - Pf1N1B4_5925 0.19 -0.0 369,724 - Pf1N1B4_5925 0.19 -0.0 369,724 - Pf1N1B4_5925 0.19 -3.2 369,724 - Pf1N1B4_5925 0.19 -1.9 369,724 - -2.1 369,726 + Pf1N1B4_5925 0.20 -0.0 369,727 - Pf1N1B4_5925 0.20 -2.6 369,727 - Pf1N1B4_5925 0.20 -0.0 369,727 - Pf1N1B4_5925 0.20 -3.2 369,908 - Pf1N1B4_5925 0.45 -2.2 369,921 - Pf1N1B4_5925 0.47 -2.2 369,921 - Pf1N1B4_5925 0.47 -4.3 369,921 - Pf1N1B4_5925 0.47 -0.0 369,921 - Pf1N1B4_5925 0.47 -1.0 369,966 - Pf1N1B4_5925 0.53 -3.2 369,978 - Pf1N1B4_5925 0.55 -2.5 369,986 - Pf1N1B4_5925 0.56 -2.7 370,024 - Pf1N1B4_5925 0.61 -1.9 370,030 + Pf1N1B4_5925 0.62 -1.8 370,031 - Pf1N1B4_5925 0.62 -2.7 370,031 - Pf1N1B4_5925 0.62 -2.1 370,031 - Pf1N1B4_5925 0.62 -1.7 370,031 - Pf1N1B4_5925 0.62 -2.4 370,078 - Pf1N1B4_5925 0.69 -0.0 370,082 - -1.9 370,082 - Pf1N1B4_5925 0.70 -1.9 370,082 - Pf1N1B4_5925 0.70 -4.1 370,082 - Pf1N1B4_5925 0.70 -2.6 370,082 - Pf1N1B4_5925 0.70 -2.6 370,082 - Pf1N1B4_5925 0.70 -2.7 370,082 - Pf1N1B4_5925 0.70 -2.2 370,082 - Pf1N1B4_5925 0.70 -2.2 370,153 - Pf1N1B4_5925 0.80 -2.6 370,153 - Pf1N1B4_5925 0.80 -1.1 370,153 - Pf1N1B4_5925 0.80 -3.3 370,153 - Pf1N1B4_5925 0.80 -0.0 370,153 - Pf1N1B4_5925 0.80 -3.4 370,272 + -1.9 370,272 + -1.8 370,273 - -2.6 370,273 - -2.9 370,273 - -0.0 370,275 + -0.0 370,276 - -2.3 370,276 - -3.0 370,276 - -4.3 370,292 + -0.0 370,293 - -0.0 370,321 - -2.7 370,321 - -3.2 370,333 + +0.8 370,334 - -0.0 370,424 + Pf1N1B4_5926 0.13 -3.2 370,424 + Pf1N1B4_5926 0.13 -2.9 370,424 + Pf1N1B4_5926 0.13 -2.9 370,424 + Pf1N1B4_5926 0.13 -3.5 370,424 + Pf1N1B4_5926 0.13 -3.8 370,425 - Pf1N1B4_5926 0.13 -2.7 370,428 + Pf1N1B4_5926 0.14 -3.3 370,428 + Pf1N1B4_5926 0.14 -3.8 370,429 - Pf1N1B4_5926 0.14 -0.0 370,429 - Pf1N1B4_5926 0.14 -3.4 370,515 + Pf1N1B4_5926 0.27 -2.9 370,516 - Pf1N1B4_5926 0.27 -3.4 370,567 + Pf1N1B4_5926 0.34 -1.9 370,575 + Pf1N1B4_5926 0.36 -1.6 370,576 - Pf1N1B4_5926 0.36 -2.1 370,576 - Pf1N1B4_5926 0.36 -5.1 370,576 - Pf1N1B4_5926 0.36 -3.9 370,576 - Pf1N1B4_5926 0.36 -2.2 370,711 + Pf1N1B4_5926 0.56 -3.3 370,711 + Pf1N1B4_5926 0.56 +0.3 370,711 + Pf1N1B4_5926 0.56 -2.5 370,712 - Pf1N1B4_5926 0.56 -3.5 370,712 - Pf1N1B4_5926 0.56 -3.4 370,762 + Pf1N1B4_5926 0.64 -1.5 370,762 + Pf1N1B4_5926 0.64 -0.0 370,762 + Pf1N1B4_5926 0.64 -3.7 370,763 - Pf1N1B4_5926 0.64 -1.9 370,825 - Pf1N1B4_5926 0.73 -0.0 370,825 - Pf1N1B4_5926 0.73 -3.4 370,825 - Pf1N1B4_5926 0.73 -3.7 370,843 + Pf1N1B4_5926 0.76 -0.4 370,843 + Pf1N1B4_5926 0.76 -2.9 370,844 - Pf1N1B4_5926 0.76 -2.1 370,844 - Pf1N1B4_5926 0.76 -1.7 370,844 - Pf1N1B4_5926 0.76 -4.0 370,848 + Pf1N1B4_5926 0.76 -2.7 370,875 - Pf1N1B4_5926 0.80 -1.9 370,875 - Pf1N1B4_5926 0.80 -2.0 370,876 + Pf1N1B4_5926 0.81 -3.2 370,876 + Pf1N1B4_5926 0.81 -2.7 370,877 - Pf1N1B4_5926 0.81 -0.0 370,877 - Pf1N1B4_5926 0.81 -2.9 370,989 + -0.0 370,989 + -0.4 370,989 + -0.0 370,990 - -2.0 370,990 - -3.2 371,059 - -0.0 371,125 - -1.7 371,247 - -3.1 371,247 - -2.5 371,247 - -2.3 371,247 - -0.0 371,263 + -0.0 371,290 - -2.0 371,296 + -2.0 371,296 + +1.0 371,296 + -0.0 371,297 - -0.0 371,297 - -0.0 371,297 - -0.9
Or see this region's nucleotide sequence