Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5776 and Pf1N1B4_5777 are separated by 115 nucleotides Pf1N1B4_5777 and Pf1N1B4_5778 are separated by 31 nucleotides Pf1N1B4_5778 and Pf1N1B4_5779 overlap by 4 nucleotides
Pf1N1B4_5776 - short-chain dehydrogenase/reductase, at 213,379 to 214,122
_5776
Pf1N1B4_5777 - Nitrous oxide reductase maturation protein, outer-membrane lipoprotein NosL, at 214,238 to 214,765
_5777
Pf1N1B4_5778 - Nitrous oxide reductase maturation transmembrane protein NosY, at 214,797 to 215,627
_5778
Pf1N1B4_5779 - Nitrous oxide reductase maturation protein NosF (ATPase), at 215,624 to 216,547
_5779
Position (kb) 214
215 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 213.251 kb on + strand at 213.251 kb on + strand at 213.324 kb on - strand at 213.324 kb on - strand at 213.327 kb on - strand at 213.349 kb on - strand at 213.349 kb on - strand at 213.352 kb on + strand at 213.352 kb on + strand at 213.353 kb on - strand at 213.353 kb on - strand at 213.353 kb on - strand at 213.353 kb on - strand at 213.721 kb on - strand, within Pf1N1B4_5776 at 213.876 kb on - strand, within Pf1N1B4_5776 at 214.175 kb on + strand at 214.175 kb on + strand at 214.197 kb on + strand at 214.198 kb on - strand at 214.370 kb on + strand, within Pf1N1B4_5777 at 214.410 kb on + strand at 214.410 kb on + strand, within Pf1N1B4_5777 at 214.411 kb on - strand, within Pf1N1B4_5777 at 214.485 kb on + strand at 214.485 kb on + strand, within Pf1N1B4_5777 at 214.486 kb on - strand, within Pf1N1B4_5777 at 214.718 kb on + strand at 214.719 kb on - strand at 214.760 kb on - strand at 214.760 kb on - strand at 214.986 kb on + strand, within Pf1N1B4_5778 at 215.143 kb on + strand, within Pf1N1B4_5778 at 215.668 kb on - strand at 215.668 kb on - strand at 215.676 kb on + strand at 215.735 kb on + strand, within Pf1N1B4_5779 at 215.736 kb on - strand, within Pf1N1B4_5779
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 213,251 + -0.0 213,251 + -0.0 213,324 - -0.0 213,324 - -2.3 213,327 - -0.0 213,349 - -1.6 213,349 - -1.9 213,352 + -2.4 213,352 + -0.4 213,353 - -1.7 213,353 - -2.3 213,353 - -0.0 213,353 - -0.0 213,721 - Pf1N1B4_5776 0.46 -0.0 213,876 - Pf1N1B4_5776 0.67 -0.0 214,175 + -0.0 214,175 + -0.9 214,197 + -0.0 214,198 - -0.0 214,370 + Pf1N1B4_5777 0.25 -0.0 214,410 + -0.0 214,410 + Pf1N1B4_5777 0.33 -0.0 214,411 - Pf1N1B4_5777 0.33 -1.8 214,485 + -1.6 214,485 + Pf1N1B4_5777 0.47 -0.0 214,486 - Pf1N1B4_5777 0.47 -3.4 214,718 + -1.2 214,719 - -0.0 214,760 - -0.0 214,760 - -1.4 214,986 + Pf1N1B4_5778 0.23 -0.0 215,143 + Pf1N1B4_5778 0.42 -0.0 215,668 - -0.0 215,668 - -1.3 215,676 + -1.2 215,735 + Pf1N1B4_5779 0.12 -0.0 215,736 - Pf1N1B4_5779 0.12 -1.4
Or see this region's nucleotide sequence