Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5738

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5736 and Pf1N1B4_5737 overlap by 23 nucleotidesPf1N1B4_5737 and Pf1N1B4_5738 overlap by 4 nucleotidesPf1N1B4_5738 and Pf1N1B4_5739 overlap by 24 nucleotidesPf1N1B4_5739 and Pf1N1B4_5740 are separated by 135 nucleotides Pf1N1B4_5736 - Nitrate/nitrite transporter, at 178,669 to 180,066 _5736 Pf1N1B4_5737 - Nitrate/nitrite transporter, at 180,044 to 180,526 _5737 Pf1N1B4_5738 - Nitrate/nitrite transporter, at 180,523 to 181,347 _5738 Pf1N1B4_5739 - hypothetical protein, at 181,324 to 181,515 _5739 Pf1N1B4_5740 - Nitrate/nitrite sensor protein (EC 2.7.3.-), at 181,651 to 183,453 _5740 Position (kb) 180 181 182Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 179.684 kb on + strand, within Pf1N1B4_5736at 179.798 kb on + strand, within Pf1N1B4_5736at 179.798 kb on + strand, within Pf1N1B4_5736at 179.799 kb on - strand, within Pf1N1B4_5736at 180.144 kb on - strand, within Pf1N1B4_5737at 180.156 kb on + strand, within Pf1N1B4_5737at 180.156 kb on + strand, within Pf1N1B4_5737at 180.156 kb on + strandat 180.157 kb on - strand, within Pf1N1B4_5737at 180.336 kb on + strand, within Pf1N1B4_5737at 180.635 kb on + strand, within Pf1N1B4_5738at 180.636 kb on - strand, within Pf1N1B4_5738at 180.647 kb on + strand, within Pf1N1B4_5738at 180.647 kb on + strand, within Pf1N1B4_5738at 180.647 kb on + strand, within Pf1N1B4_5738at 180.648 kb on - strand, within Pf1N1B4_5738at 180.648 kb on - strand, within Pf1N1B4_5738at 180.648 kb on - strand, within Pf1N1B4_5738at 180.648 kb on - strand, within Pf1N1B4_5738at 180.650 kb on + strand, within Pf1N1B4_5738at 180.650 kb on + strand, within Pf1N1B4_5738at 180.650 kb on + strand, within Pf1N1B4_5738at 180.650 kb on + strand, within Pf1N1B4_5738at 180.651 kb on - strand, within Pf1N1B4_5738at 180.651 kb on - strandat 180.651 kb on - strand, within Pf1N1B4_5738at 180.704 kb on + strand, within Pf1N1B4_5738at 180.705 kb on - strand, within Pf1N1B4_5738at 180.986 kb on + strand, within Pf1N1B4_5738at 180.986 kb on + strand, within Pf1N1B4_5738at 180.987 kb on - strand, within Pf1N1B4_5738at 180.987 kb on - strand, within Pf1N1B4_5738at 180.987 kb on - strand, within Pf1N1B4_5738at 181.331 kb on - strandat 181.331 kb on - strandat 181.331 kb on - strandat 181.382 kb on + strand, within Pf1N1B4_5739at 181.444 kb on - strand, within Pf1N1B4_5739at 181.564 kb on - strandat 181.564 kb on - strandat 181.958 kb on - strand, within Pf1N1B4_5740at 181.958 kb on - strand, within Pf1N1B4_5740at 181.958 kb on - strand, within Pf1N1B4_5740at 181.992 kb on - strand, within Pf1N1B4_5740at 182.181 kb on - strand, within Pf1N1B4_5740at 182.185 kb on + strand, within Pf1N1B4_5740

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
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179,684 + Pf1N1B4_5736 0.73 -0.0
179,798 + Pf1N1B4_5736 0.81 -0.0
179,798 + Pf1N1B4_5736 0.81 -2.9
179,799 - Pf1N1B4_5736 0.81 -0.0
180,144 - Pf1N1B4_5737 0.21 -0.0
180,156 + Pf1N1B4_5737 0.23 -0.2
180,156 + Pf1N1B4_5737 0.23 -0.0
180,156 + -0.0
180,157 - Pf1N1B4_5737 0.23 -2.8
180,336 + Pf1N1B4_5737 0.60 -0.4
180,635 + Pf1N1B4_5738 0.14 -0.0
180,636 - Pf1N1B4_5738 0.14 -3.7
180,647 + Pf1N1B4_5738 0.15 -0.0
180,647 + Pf1N1B4_5738 0.15 -0.0
180,647 + Pf1N1B4_5738 0.15 -0.0
180,648 - Pf1N1B4_5738 0.15 -2.1
180,648 - Pf1N1B4_5738 0.15 -0.0
180,648 - Pf1N1B4_5738 0.15 -0.0
180,648 - Pf1N1B4_5738 0.15 -1.7
180,650 + Pf1N1B4_5738 0.15 -0.0
180,650 + Pf1N1B4_5738 0.15 -0.0
180,650 + Pf1N1B4_5738 0.15 -1.9
180,650 + Pf1N1B4_5738 0.15 -2.9
180,651 - Pf1N1B4_5738 0.16 -3.2
180,651 - -5.1
180,651 - Pf1N1B4_5738 0.16 -0.0
180,704 + Pf1N1B4_5738 0.22 -2.3
180,705 - Pf1N1B4_5738 0.22 -0.0
180,986 + Pf1N1B4_5738 0.56 -2.1
180,986 + Pf1N1B4_5738 0.56 -0.0
180,987 - Pf1N1B4_5738 0.56 -2.0
180,987 - Pf1N1B4_5738 0.56 -0.0
180,987 - Pf1N1B4_5738 0.56 -0.0
181,331 - -0.0
181,331 - -1.1
181,331 - +0.9
181,382 + Pf1N1B4_5739 0.30 -0.0
181,444 - Pf1N1B4_5739 0.62 -1.4
181,564 - -1.4
181,564 - -0.0
181,958 - Pf1N1B4_5740 0.17 -0.0
181,958 - Pf1N1B4_5740 0.17 -0.0
181,958 - Pf1N1B4_5740 0.17 -1.7
181,992 - Pf1N1B4_5740 0.19 -1.8
182,181 - Pf1N1B4_5740 0.29 -0.0
182,185 + Pf1N1B4_5740 0.30 -0.0

Or see this region's nucleotide sequence