Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5725

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5722 and Pf1N1B4_5723 are separated by 0 nucleotidesPf1N1B4_5723 and Pf1N1B4_5724 are separated by 34 nucleotidesPf1N1B4_5724 and Pf1N1B4_5725 overlap by 16 nucleotidesPf1N1B4_5725 and Pf1N1B4_5726 are separated by 38 nucleotides Pf1N1B4_5722 - Periplasmic protein p19 involved in high-affinity Fe2+ transport, at 163,711 to 164,091 _5722 Pf1N1B4_5723 - putative exported protein, at 164,092 to 164,424 _5723 Pf1N1B4_5724 - High-affinity iron permease, at 164,459 to 164,941 _5724 Pf1N1B4_5725 - High-affinity iron permease, at 164,926 to 165,300 _5725 Pf1N1B4_5726 - Ferredoxin, at 165,339 to 166,667 _5726 Position (kb) 164 165 166Strain fitness (log2 ratio) -2 -1 0 1at 163.936 kb on - strand, within Pf1N1B4_5722at 164.798 kb on + strand, within Pf1N1B4_5724at 164.798 kb on + strand, within Pf1N1B4_5724at 164.798 kb on + strand, within Pf1N1B4_5724at 164.801 kb on + strand, within Pf1N1B4_5724at 164.802 kb on - strand, within Pf1N1B4_5724at 165.101 kb on - strand, within Pf1N1B4_5725at 165.385 kb on + strandat 165.394 kb on - strandat 165.485 kb on - strand, within Pf1N1B4_5726at 165.594 kb on + strand, within Pf1N1B4_5726at 165.699 kb on + strand, within Pf1N1B4_5726at 166.054 kb on + strand, within Pf1N1B4_5726

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
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163,936 - Pf1N1B4_5722 0.59 -0.0
164,798 + Pf1N1B4_5724 0.70 -0.0
164,798 + Pf1N1B4_5724 0.70 -0.9
164,798 + Pf1N1B4_5724 0.70 -0.0
164,801 + Pf1N1B4_5724 0.71 -0.2
164,802 - Pf1N1B4_5724 0.71 -1.2
165,101 - Pf1N1B4_5725 0.47 -0.1
165,385 + -2.1
165,394 - -0.0
165,485 - Pf1N1B4_5726 0.11 -1.0
165,594 + Pf1N1B4_5726 0.19 -1.5
165,699 + Pf1N1B4_5726 0.27 -0.0
166,054 + Pf1N1B4_5726 0.54 -1.3

Or see this region's nucleotide sequence