Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5519

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5518 and Pf1N1B4_5519 are separated by 595 nucleotidesPf1N1B4_5519 and Pf1N1B4_5520 are separated by 485 nucleotides Pf1N1B4_5518 - General secretion pathway protein E, at 270,870 to 271,898 _5518 Pf1N1B4_5519 - hypothetical protein, at 272,494 to 273,657 _5519 Pf1N1B4_5520 - General secretion pathway protein E, at 274,143 to 275,993 _5520 Position (kb) 272 273 274Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 271.496 kb on + strand, within Pf1N1B4_5518at 271.497 kb on - strand, within Pf1N1B4_5518at 271.537 kb on - strand, within Pf1N1B4_5518at 271.538 kb on + strand, within Pf1N1B4_5518at 271.826 kb on - strandat 271.845 kb on - strandat 272.045 kb on - strandat 272.130 kb on - strandat 272.130 kb on - strandat 272.226 kb on + strandat 272.260 kb on - strandat 272.330 kb on - strandat 272.331 kb on + strandat 272.564 kb on - strandat 272.564 kb on - strandat 272.625 kb on - strand, within Pf1N1B4_5519at 272.780 kb on - strand, within Pf1N1B4_5519at 272.793 kb on - strand, within Pf1N1B4_5519at 273.029 kb on + strandat 273.030 kb on - strand, within Pf1N1B4_5519at 273.225 kb on + strand, within Pf1N1B4_5519at 273.245 kb on + strand, within Pf1N1B4_5519at 273.406 kb on - strand, within Pf1N1B4_5519at 273.679 kb on - strandat 273.791 kb on - strandat 273.861 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
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271,496 + Pf1N1B4_5518 0.61 -0.0
271,497 - Pf1N1B4_5518 0.61 -0.0
271,537 - Pf1N1B4_5518 0.65 -1.9
271,538 + Pf1N1B4_5518 0.65 -0.0
271,826 - -2.0
271,845 - -1.7
272,045 - -0.0
272,130 - -0.0
272,130 - -0.0
272,226 + -0.0
272,260 - -0.0
272,330 - -0.0
272,331 + -0.0
272,564 - -0.3
272,564 - -2.4
272,625 - Pf1N1B4_5519 0.11 -0.0
272,780 - Pf1N1B4_5519 0.25 -2.7
272,793 - Pf1N1B4_5519 0.26 -0.0
273,029 + -2.0
273,030 - Pf1N1B4_5519 0.46 -0.0
273,225 + Pf1N1B4_5519 0.63 -4.6
273,245 + Pf1N1B4_5519 0.65 -0.0
273,406 - Pf1N1B4_5519 0.78 -0.0
273,679 - -2.7
273,791 - -2.2
273,861 - -4.8

Or see this region's nucleotide sequence