Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5504 and Pf1N1B4_5505 are separated by 38 nucleotides Pf1N1B4_5505 and Pf1N1B4_5506 overlap by 10 nucleotides Pf1N1B4_5506 and Pf1N1B4_5507 are separated by 48 nucleotides
Pf1N1B4_5504 - hypothetical protein, at 254,478 to 255,089
_5504
Pf1N1B4_5505 - hypothetical protein, at 255,128 to 255,304
_5505
Pf1N1B4_5506 - hypothetical protein, at 255,295 to 255,819
_5506
Pf1N1B4_5507 - hypothetical protein, at 255,868 to 256,542
_5507
Position (kb) 255
256
257 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 254.893 kb on + strand, within Pf1N1B4_5504 at 254.894 kb on - strand, within Pf1N1B4_5504 at 254.894 kb on - strand, within Pf1N1B4_5504 at 255.054 kb on + strand at 255.055 kb on - strand at 255.066 kb on + strand at 255.067 kb on - strand at 255.067 kb on - strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.243 kb on - strand, within Pf1N1B4_5505 at 255.243 kb on - strand at 255.269 kb on + strand at 255.269 kb on + strand at 255.269 kb on + strand, within Pf1N1B4_5505 at 255.269 kb on + strand, within Pf1N1B4_5505 at 255.270 kb on - strand, within Pf1N1B4_5505 at 255.270 kb on - strand, within Pf1N1B4_5505 at 255.289 kb on + strand at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.592 kb on + strand at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.593 kb on - strand, within Pf1N1B4_5506 at 255.593 kb on - strand, within Pf1N1B4_5506 at 255.593 kb on - strand at 255.593 kb on - strand at 255.689 kb on + strand, within Pf1N1B4_5506 at 255.689 kb on + strand, within Pf1N1B4_5506 at 255.689 kb on + strand, within Pf1N1B4_5506 at 255.689 kb on + strand, within Pf1N1B4_5506 at 255.691 kb on + strand at 255.692 kb on - strand, within Pf1N1B4_5506 at 255.740 kb on + strand, within Pf1N1B4_5506 at 255.741 kb on - strand, within Pf1N1B4_5506 at 255.741 kb on - strand, within Pf1N1B4_5506 at 255.829 kb on + strand at 255.829 kb on + strand at 255.829 kb on + strand at 255.830 kb on - strand at 255.881 kb on + strand at 255.881 kb on + strand at 255.881 kb on + strand at 255.882 kb on - strand at 255.932 kb on + strand at 255.933 kb on - strand at 255.933 kb on - strand at 255.933 kb on - strand at 255.933 kb on - strand at 255.933 kb on - strand at 255.933 kb on - strand at 256.353 kb on - strand, within Pf1N1B4_5507 at 256.405 kb on + strand, within Pf1N1B4_5507 at 256.405 kb on + strand, within Pf1N1B4_5507 at 256.406 kb on - strand, within Pf1N1B4_5507 at 256.490 kb on + strand at 256.491 kb on - strand at 256.491 kb on - strand at 256.662 kb on + strand at 256.662 kb on + strand at 256.680 kb on - strand at 256.720 kb on + strand at 256.720 kb on + strand at 256.721 kb on - strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.852 kb on - strand at 256.852 kb on - strand at 256.852 kb on - strand at 256.852 kb on - strand at 256.852 kb on - strand at 256.956 kb on + strand at 256.956 kb on + strand at 256.956 kb on + strand at 256.956 kb on + strand at 256.990 kb on + strand at 256.990 kb on + strand at 256.990 kb on + strand at 256.991 kb on - strand at 257.022 kb on + strand at 257.048 kb on + strand at 257.048 kb on + strand at 257.094 kb on + strand at 257.095 kb on - strand at 257.095 kb on - strand at 257.095 kb on - strand at 257.134 kb on + strand at 257.134 kb on + strand at 257.134 kb on + strand at 257.134 kb on + strand at 257.135 kb on - strand at 257.135 kb on - strand at 257.135 kb on - strand at 257.135 kb on - strand at 257.135 kb on - strand at 257.135 kb on - strand at 257.135 kb on - strand at 257.156 kb on - strand at 257.177 kb on - strand at 257.186 kb on - strand at 257.231 kb on - strand at 257.304 kb on - strand at 257.372 kb on + strand at 257.372 kb on + strand at 257.373 kb on - strand at 257.373 kb on - strand at 257.373 kb on - strand at 257.373 kb on - strand at 257.373 kb on - strand at 257.373 kb on - strand at 257.373 kb on - strand at 257.389 kb on - strand at 257.389 kb on - strand at 257.389 kb on - strand at 257.421 kb on + strand at 257.422 kb on - strand at 257.422 kb on - strand at 257.466 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 254,893 + Pf1N1B4_5504 0.68 -0.0 254,894 - Pf1N1B4_5504 0.68 -2.4 254,894 - Pf1N1B4_5504 0.68 -0.0 255,054 + -0.0 255,055 - -1.7 255,066 + -1.5 255,067 - -1.2 255,067 - -0.0 255,131 + -0.0 255,131 + -0.7 255,131 + -0.0 255,131 + -0.9 255,131 + -2.7 255,131 + -0.0 255,131 + -0.0 255,132 - -1.0 255,132 - -0.0 255,132 - -0.0 255,132 - -2.6 255,132 - -1.4 255,132 - -0.0 255,132 - -0.0 255,242 + Pf1N1B4_5505 0.64 -0.0 255,242 + Pf1N1B4_5505 0.64 -0.0 255,242 + -0.0 255,242 + Pf1N1B4_5505 0.64 -3.2 255,242 + Pf1N1B4_5505 0.64 -0.0 255,242 + -1.4 255,242 + Pf1N1B4_5505 0.64 -1.7 255,242 + Pf1N1B4_5505 0.64 -0.0 255,242 + Pf1N1B4_5505 0.64 -1.7 255,243 - Pf1N1B4_5505 0.65 -1.5 255,243 - -2.5 255,269 + -2.3 255,269 + -0.0 255,269 + Pf1N1B4_5505 0.80 -0.0 255,269 + Pf1N1B4_5505 0.80 -0.0 255,270 - Pf1N1B4_5505 0.80 -1.6 255,270 - Pf1N1B4_5505 0.80 -0.0 255,289 + -0.0 255,592 + Pf1N1B4_5506 0.57 -2.3 255,592 + Pf1N1B4_5506 0.57 -0.0 255,592 + Pf1N1B4_5506 0.57 -0.0 255,592 + Pf1N1B4_5506 0.57 -0.0 255,592 + -0.0 255,592 + Pf1N1B4_5506 0.57 -1.7 255,593 - Pf1N1B4_5506 0.57 -1.7 255,593 - Pf1N1B4_5506 0.57 -2.1 255,593 - -1.1 255,593 - -0.0 255,689 + Pf1N1B4_5506 0.75 -1.9 255,689 + Pf1N1B4_5506 0.75 -2.0 255,689 + Pf1N1B4_5506 0.75 -0.0 255,689 + Pf1N1B4_5506 0.75 -0.0 255,691 + -2.3 255,692 - Pf1N1B4_5506 0.76 -2.2 255,740 + Pf1N1B4_5506 0.85 -0.0 255,741 - Pf1N1B4_5506 0.85 -1.3 255,741 - Pf1N1B4_5506 0.85 -0.0 255,829 + -0.0 255,829 + -0.0 255,829 + -0.6 255,830 - -2.2 255,881 + -0.0 255,881 + -3.5 255,881 + -0.0 255,882 - -0.0 255,932 + -0.0 255,933 - -0.0 255,933 - -0.0 255,933 - -0.0 255,933 - -1.3 255,933 - -2.0 255,933 - -0.0 256,353 - Pf1N1B4_5507 0.72 -2.6 256,405 + Pf1N1B4_5507 0.80 -0.0 256,405 + Pf1N1B4_5507 0.80 -0.0 256,406 - Pf1N1B4_5507 0.80 -0.0 256,490 + -0.0 256,491 - -1.2 256,491 - -0.0 256,662 + -0.0 256,662 + -0.0 256,680 - -1.4 256,720 + -0.0 256,720 + -0.0 256,721 - -1.9 256,851 + -0.0 256,851 + -0.0 256,851 + -2.3 256,851 + -1.5 256,851 + -1.8 256,851 + -1.6 256,851 + -0.0 256,851 + -0.0 256,851 + -0.0 256,851 + -0.4 256,851 + -2.4 256,852 - +1.0 256,852 - -0.0 256,852 - -0.0 256,852 - -0.0 256,852 - -0.0 256,956 + -0.0 256,956 + -2.6 256,956 + -0.0 256,956 + -3.0 256,990 + -1.1 256,990 + -0.0 256,990 + -0.0 256,991 - -0.0 257,022 + -2.4 257,048 + -0.0 257,048 + -0.0 257,094 + -0.0 257,095 - -0.0 257,095 - -2.7 257,095 - -1.8 257,134 + -0.9 257,134 + -0.0 257,134 + -2.1 257,134 + -2.1 257,135 - -0.0 257,135 - -0.0 257,135 - -1.4 257,135 - -0.0 257,135 - -0.0 257,135 - -2.4 257,135 - -0.0 257,156 - -0.0 257,177 - -2.1 257,186 - -1.5 257,231 - -0.0 257,304 - -1.6 257,372 + -2.4 257,372 + -2.0 257,373 - -0.0 257,373 - -0.0 257,373 - -0.0 257,373 - -0.0 257,373 - -1.6 257,373 - -0.0 257,373 - -1.0 257,389 - -1.8 257,389 - -0.8 257,389 - -0.0 257,421 + -0.0 257,422 - -0.0 257,422 - -1.6 257,466 + -0.0
Or see this region's nucleotide sequence