Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5388 and Pf1N1B4_5389 are separated by 566 nucleotides Pf1N1B4_5389 and Pf1N1B4_5390 are separated by 455 nucleotides Pf1N1B4_5390 and Pf1N1B4_5391 are separated by 100 nucleotides
Pf1N1B4_5388 - hypothetical protein, at 119,847 to 120,035
_5388
Pf1N1B4_5389 - hypothetical protein, at 120,602 to 121,195
_5389
Pf1N1B4_5390 - hypothetical protein, at 121,651 to 121,899
_5390
Pf1N1B4_5391 - hypothetical protein, at 122,000 to 123,037
_5391
Position (kb) 120
121
122 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 119.760 kb on + strand at 119.761 kb on - strand at 119.811 kb on + strand at 120.164 kb on - strand at 120.203 kb on + strand at 120.203 kb on + strand at 120.319 kb on + strand at 120.319 kb on + strand at 120.319 kb on + strand at 120.319 kb on + strand at 120.319 kb on + strand at 120.319 kb on + strand at 120.320 kb on - strand at 120.320 kb on - strand at 120.320 kb on - strand at 120.320 kb on - strand at 120.407 kb on - strand at 120.407 kb on - strand at 120.483 kb on + strand at 120.501 kb on + strand at 120.502 kb on - strand at 120.502 kb on - strand at 120.627 kb on - strand at 120.642 kb on + strand at 120.642 kb on + strand at 120.643 kb on - strand at 120.643 kb on - strand at 120.643 kb on - strand at 120.643 kb on - strand at 120.643 kb on - strand at 120.643 kb on - strand at 120.643 kb on - strand at 120.667 kb on - strand, within Pf1N1B4_5389 at 120.707 kb on + strand, within Pf1N1B4_5389 at 120.823 kb on + strand, within Pf1N1B4_5389 at 120.936 kb on + strand, within Pf1N1B4_5389 at 120.936 kb on + strand, within Pf1N1B4_5389 at 120.936 kb on + strand, within Pf1N1B4_5389 at 121.000 kb on - strand at 121.042 kb on + strand, within Pf1N1B4_5389 at 121.042 kb on + strand, within Pf1N1B4_5389 at 121.042 kb on + strand, within Pf1N1B4_5389 at 121.043 kb on - strand at 121.043 kb on - strand, within Pf1N1B4_5389 at 121.050 kb on + strand, within Pf1N1B4_5389 at 121.051 kb on - strand, within Pf1N1B4_5389 at 121.051 kb on - strand, within Pf1N1B4_5389 at 121.077 kb on + strand, within Pf1N1B4_5389 at 121.077 kb on + strand, within Pf1N1B4_5389 at 121.077 kb on + strand, within Pf1N1B4_5389 at 121.078 kb on - strand, within Pf1N1B4_5389 at 121.078 kb on - strand, within Pf1N1B4_5389 at 121.078 kb on - strand, within Pf1N1B4_5389 at 121.157 kb on - strand at 121.364 kb on + strand at 121.364 kb on + strand at 121.364 kb on + strand at 121.399 kb on - strand at 121.606 kb on + strand at 121.607 kb on - strand at 121.659 kb on + strand at 121.941 kb on + strand at 121.942 kb on - strand at 121.944 kb on + strand at 122.077 kb on - strand at 122.154 kb on + strand, within Pf1N1B4_5391 at 122.155 kb on - strand, within Pf1N1B4_5391
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 119,760 + -0.0 119,761 - -0.0 119,811 + -0.0 120,164 - -0.0 120,203 + -2.4 120,203 + -0.0 120,319 + -0.0 120,319 + -1.9 120,319 + -0.0 120,319 + -2.3 120,319 + -0.0 120,319 + -3.0 120,320 - -0.0 120,320 - -0.0 120,320 - -0.0 120,320 - -0.0 120,407 - -1.5 120,407 - -1.3 120,483 + -0.7 120,501 + -0.0 120,502 - -0.0 120,502 - -2.2 120,627 - -2.7 120,642 + -0.0 120,642 + -1.0 120,643 - -0.0 120,643 - -1.2 120,643 - -0.9 120,643 - -0.0 120,643 - -5.1 120,643 - -1.6 120,643 - -0.0 120,667 - Pf1N1B4_5389 0.11 -0.0 120,707 + Pf1N1B4_5389 0.18 -0.0 120,823 + Pf1N1B4_5389 0.37 -3.3 120,936 + Pf1N1B4_5389 0.56 -0.0 120,936 + Pf1N1B4_5389 0.56 -0.0 120,936 + Pf1N1B4_5389 0.56 -0.0 121,000 - -1.4 121,042 + Pf1N1B4_5389 0.74 -0.0 121,042 + Pf1N1B4_5389 0.74 -0.0 121,042 + Pf1N1B4_5389 0.74 -0.0 121,043 - -0.0 121,043 - Pf1N1B4_5389 0.74 -0.0 121,050 + Pf1N1B4_5389 0.75 -1.7 121,051 - Pf1N1B4_5389 0.76 -0.0 121,051 - Pf1N1B4_5389 0.76 -0.0 121,077 + Pf1N1B4_5389 0.80 -0.0 121,077 + Pf1N1B4_5389 0.80 -2.3 121,077 + Pf1N1B4_5389 0.80 -3.1 121,078 - Pf1N1B4_5389 0.80 -0.0 121,078 - Pf1N1B4_5389 0.80 -2.3 121,078 - Pf1N1B4_5389 0.80 -0.0 121,157 - -0.0 121,364 + -0.0 121,364 + -2.0 121,364 + -0.0 121,399 - -0.0 121,606 + -0.0 121,607 - -0.0 121,659 + -2.5 121,941 + -0.0 121,942 - -1.4 121,944 + -0.0 122,077 - -2.9 122,154 + Pf1N1B4_5391 0.15 -0.0 122,155 - Pf1N1B4_5391 0.15 -1.4
Or see this region's nucleotide sequence