Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5379 and Pf1N1B4_5380 are separated by 125 nucleotides Pf1N1B4_5380 and Pf1N1B4_5381 are separated by 266 nucleotides Pf1N1B4_5381 and Pf1N1B4_5382 are separated by 486 nucleotides
Pf1N1B4_5379 - hypothetical protein, at 113,039 to 113,362
_5379
Pf1N1B4_5380 - hypothetical protein, at 113,488 to 113,916
_5380
Pf1N1B4_5381 - hypothetical protein, at 114,183 to 114,464
_5381
Pf1N1B4_5382 - hypothetical protein, at 114,951 to 115,394
_5382
Position (kb)
114
115 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 113.360 kb on + strand at 113.360 kb on + strand at 113.361 kb on - strand at 113.372 kb on + strand at 113.407 kb on + strand at 113.428 kb on - strand at 113.629 kb on + strand, within Pf1N1B4_5380 at 113.629 kb on + strand, within Pf1N1B4_5380 at 113.629 kb on + strand, within Pf1N1B4_5380 at 113.629 kb on + strand at 113.630 kb on - strand, within Pf1N1B4_5380 at 113.632 kb on + strand, within Pf1N1B4_5380 at 113.632 kb on + strand, within Pf1N1B4_5380 at 113.633 kb on - strand, within Pf1N1B4_5380 at 113.633 kb on - strand, within Pf1N1B4_5380 at 113.659 kb on + strand, within Pf1N1B4_5380 at 113.659 kb on + strand, within Pf1N1B4_5380 at 113.659 kb on + strand, within Pf1N1B4_5380 at 113.659 kb on + strand, within Pf1N1B4_5380 at 113.660 kb on - strand at 113.682 kb on + strand, within Pf1N1B4_5380 at 113.721 kb on + strand, within Pf1N1B4_5380 at 113.758 kb on + strand, within Pf1N1B4_5380 at 113.944 kb on + strand at 113.944 kb on + strand at 113.944 kb on + strand at 113.944 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.013 kb on - strand at 114.013 kb on - strand at 114.013 kb on - strand at 114.035 kb on + strand at 114.101 kb on + strand at 114.109 kb on - strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.144 kb on - strand at 114.144 kb on - strand at 114.144 kb on - strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.148 kb on - strand at 114.148 kb on - strand at 114.148 kb on - strand at 114.148 kb on - strand at 114.163 kb on + strand at 114.163 kb on + strand at 114.163 kb on + strand at 114.164 kb on - strand at 114.181 kb on + strand at 114.181 kb on + strand at 114.281 kb on + strand, within Pf1N1B4_5381 at 114.286 kb on + strand, within Pf1N1B4_5381 at 114.286 kb on + strand, within Pf1N1B4_5381 at 114.320 kb on + strand, within Pf1N1B4_5381 at 114.321 kb on - strand, within Pf1N1B4_5381 at 114.343 kb on + strand at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.400 kb on + strand, within Pf1N1B4_5381 at 114.400 kb on + strand, within Pf1N1B4_5381 at 114.400 kb on + strand, within Pf1N1B4_5381 at 114.400 kb on + strand, within Pf1N1B4_5381 at 114.400 kb on + strand, within Pf1N1B4_5381 at 114.400 kb on + strand, within Pf1N1B4_5381 at 114.400 kb on + strand, within Pf1N1B4_5381 at 114.400 kb on + strand, within Pf1N1B4_5381 at 114.401 kb on - strand at 114.404 kb on + strand, within Pf1N1B4_5381 at 114.404 kb on + strand, within Pf1N1B4_5381 at 114.404 kb on + strand, within Pf1N1B4_5381 at 114.404 kb on + strand, within Pf1N1B4_5381 at 114.404 kb on + strand, within Pf1N1B4_5381 at 114.404 kb on + strand, within Pf1N1B4_5381 at 114.404 kb on + strand, within Pf1N1B4_5381 at 114.405 kb on - strand, within Pf1N1B4_5381 at 114.405 kb on - strand, within Pf1N1B4_5381 at 114.434 kb on + strand, within Pf1N1B4_5381 at 114.434 kb on + strand, within Pf1N1B4_5381 at 114.532 kb on + strand at 114.620 kb on + strand at 114.620 kb on + strand at 114.637 kb on - strand at 114.684 kb on + strand at 114.797 kb on + strand at 114.797 kb on + strand at 114.994 kb on + strand at 114.995 kb on - strand at 114.995 kb on - strand at 115.027 kb on + strand, within Pf1N1B4_5382 at 115.028 kb on - strand, within Pf1N1B4_5382 at 115.210 kb on + strand, within Pf1N1B4_5382 at 115.211 kb on - strand, within Pf1N1B4_5382 at 115.211 kb on - strand, within Pf1N1B4_5382 at 115.298 kb on + strand, within Pf1N1B4_5382 at 115.299 kb on - strand, within Pf1N1B4_5382 at 115.299 kb on - strand, within Pf1N1B4_5382 at 115.406 kb on + strand at 115.406 kb on + strand at 115.415 kb on + strand at 115.415 kb on + strand at 115.415 kb on + strand at 115.416 kb on - strand at 115.434 kb on + strand at 115.434 kb on + strand at 115.434 kb on + strand at 115.434 kb on + strand at 115.434 kb on + strand at 115.434 kb on + strand at 115.434 kb on + strand at 115.434 kb on + strand at 115.434 kb on + strand at 115.435 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 113,360 + -0.0 113,360 + -0.0 113,361 - -0.0 113,372 + -0.0 113,407 + -0.0 113,428 - -1.6 113,629 + Pf1N1B4_5380 0.33 -0.0 113,629 + Pf1N1B4_5380 0.33 -1.8 113,629 + Pf1N1B4_5380 0.33 -2.0 113,629 + -0.0 113,630 - Pf1N1B4_5380 0.33 -1.5 113,632 + Pf1N1B4_5380 0.34 -1.6 113,632 + Pf1N1B4_5380 0.34 -0.0 113,633 - Pf1N1B4_5380 0.34 -1.8 113,633 - Pf1N1B4_5380 0.34 -0.0 113,659 + Pf1N1B4_5380 0.40 -1.9 113,659 + Pf1N1B4_5380 0.40 -0.0 113,659 + Pf1N1B4_5380 0.40 -2.7 113,659 + Pf1N1B4_5380 0.40 -0.0 113,660 - -1.5 113,682 + Pf1N1B4_5380 0.45 -1.6 113,721 + Pf1N1B4_5380 0.54 -1.6 113,758 + Pf1N1B4_5380 0.63 -0.0 113,944 + -0.0 113,944 + -0.0 113,944 + -0.0 113,944 + -3.5 114,012 + -1.1 114,012 + -0.0 114,012 + -0.0 114,012 + -0.0 114,012 + -0.0 114,012 + -0.0 114,012 + -0.0 114,013 - -2.0 114,013 - -0.0 114,013 - -0.6 114,035 + -0.0 114,101 + -0.0 114,109 - -0.7 114,143 + -0.0 114,143 + -0.0 114,143 + -0.0 114,143 + -0.0 114,143 + -0.0 114,143 + -0.0 114,143 + -0.0 114,143 + -0.0 114,143 + -1.3 114,143 + -1.9 114,143 + -0.0 114,143 + -0.0 114,143 + -2.2 114,143 + -0.0 114,143 + -0.0 114,143 + -0.0 114,143 + -0.0 114,144 - -0.0 114,144 - -0.0 114,144 - -2.1 114,147 + -0.0 114,147 + -0.0 114,147 + -0.0 114,147 + -0.6 114,147 + -0.0 114,147 + -2.6 114,147 + -0.0 114,147 + -0.0 114,147 + -0.0 114,147 + -0.0 114,147 + -0.0 114,148 - -0.0 114,148 - -1.3 114,148 - -0.0 114,148 - -0.0 114,163 + -0.0 114,163 + -0.0 114,163 + -0.0 114,164 - -0.8 114,181 + -0.0 114,181 + -0.0 114,281 + Pf1N1B4_5381 0.35 -2.7 114,286 + Pf1N1B4_5381 0.37 -0.0 114,286 + Pf1N1B4_5381 0.37 -4.5 114,320 + Pf1N1B4_5381 0.49 -0.0 114,321 - Pf1N1B4_5381 0.49 -0.0 114,343 + -4.3 114,343 + Pf1N1B4_5381 0.57 -0.0 114,343 + Pf1N1B4_5381 0.57 -0.0 114,343 + Pf1N1B4_5381 0.57 -1.9 114,343 + Pf1N1B4_5381 0.57 -0.0 114,343 + Pf1N1B4_5381 0.57 -0.0 114,343 + Pf1N1B4_5381 0.57 -0.0 114,343 + Pf1N1B4_5381 0.57 -0.0 114,343 + Pf1N1B4_5381 0.57 +0.6 114,400 + Pf1N1B4_5381 0.77 -0.0 114,400 + Pf1N1B4_5381 0.77 -3.1 114,400 + Pf1N1B4_5381 0.77 -1.4 114,400 + Pf1N1B4_5381 0.77 -0.0 114,400 + Pf1N1B4_5381 0.77 -0.0 114,400 + Pf1N1B4_5381 0.77 -0.0 114,400 + Pf1N1B4_5381 0.77 -0.0 114,400 + Pf1N1B4_5381 0.77 -0.0 114,401 - -1.6 114,404 + Pf1N1B4_5381 0.78 -0.0 114,404 + Pf1N1B4_5381 0.78 -0.0 114,404 + Pf1N1B4_5381 0.78 -0.0 114,404 + Pf1N1B4_5381 0.78 -0.0 114,404 + Pf1N1B4_5381 0.78 -0.0 114,404 + Pf1N1B4_5381 0.78 -2.2 114,404 + Pf1N1B4_5381 0.78 -0.0 114,405 - Pf1N1B4_5381 0.79 -0.0 114,405 - Pf1N1B4_5381 0.79 -0.0 114,434 + Pf1N1B4_5381 0.89 -1.8 114,434 + Pf1N1B4_5381 0.89 -1.2 114,532 + -0.0 114,620 + -0.0 114,620 + -0.0 114,637 - -2.8 114,684 + -0.7 114,797 + -0.0 114,797 + -0.7 114,994 + -0.0 114,995 - -0.0 114,995 - -2.7 115,027 + Pf1N1B4_5382 0.17 -0.2 115,028 - Pf1N1B4_5382 0.17 -0.0 115,210 + Pf1N1B4_5382 0.58 -0.0 115,211 - Pf1N1B4_5382 0.59 -0.0 115,211 - Pf1N1B4_5382 0.59 -0.0 115,298 + Pf1N1B4_5382 0.78 -0.0 115,299 - Pf1N1B4_5382 0.78 -0.0 115,299 - Pf1N1B4_5382 0.78 -0.0 115,406 + -1.6 115,406 + -0.0 115,415 + -0.0 115,415 + -0.0 115,415 + -0.9 115,416 - -0.0 115,434 + -0.0 115,434 + -2.6 115,434 + -0.0 115,434 + -0.0 115,434 + -0.0 115,434 + -0.0 115,434 + -3.7 115,434 + -0.0 115,434 + -0.0 115,435 - -0.0
Or see this region's nucleotide sequence