Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5378 and Pf1N1B4_5379 are separated by 756 nucleotides Pf1N1B4_5379 and Pf1N1B4_5380 are separated by 125 nucleotides Pf1N1B4_5380 and Pf1N1B4_5381 are separated by 266 nucleotides
Pf1N1B4_5378 - Phage protein, at 111,089 to 112,282
_5378
Pf1N1B4_5379 - hypothetical protein, at 113,039 to 113,362
_5379
Pf1N1B4_5380 - hypothetical protein, at 113,488 to 113,916
_5380
Pf1N1B4_5381 - hypothetical protein, at 114,183 to 114,464
_5381
Position (kb)
113
114 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 112.088 kb on + strand, within Pf1N1B4_5378 at 112.088 kb on + strand, within Pf1N1B4_5378 at 112.088 kb on + strand, within Pf1N1B4_5378 at 112.088 kb on + strand, within Pf1N1B4_5378 at 112.088 kb on + strand, within Pf1N1B4_5378 at 112.088 kb on + strand at 112.089 kb on - strand, within Pf1N1B4_5378 at 112.089 kb on - strand, within Pf1N1B4_5378 at 112.089 kb on - strand, within Pf1N1B4_5378 at 112.089 kb on - strand, within Pf1N1B4_5378 at 112.089 kb on - strand, within Pf1N1B4_5378 at 112.332 kb on + strand at 112.332 kb on + strand at 112.333 kb on - strand at 112.333 kb on - strand at 112.333 kb on - strand at 112.347 kb on + strand at 112.347 kb on + strand at 112.347 kb on + strand at 112.347 kb on + strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.386 kb on - strand at 112.427 kb on + strand at 112.427 kb on + strand at 112.431 kb on + strand at 112.431 kb on + strand at 112.431 kb on + strand at 112.431 kb on + strand at 112.431 kb on + strand at 112.431 kb on + strand at 112.431 kb on + strand at 112.432 kb on - strand at 112.432 kb on - strand at 112.432 kb on - strand at 112.432 kb on - strand at 112.432 kb on - strand at 112.432 kb on - strand at 112.570 kb on + strand at 112.570 kb on + strand at 112.570 kb on + strand at 112.571 kb on - strand at 112.571 kb on - strand at 112.636 kb on + strand at 112.636 kb on + strand at 112.649 kb on + strand at 112.649 kb on + strand at 112.650 kb on - strand at 112.650 kb on - strand at 112.650 kb on - strand at 112.686 kb on + strand at 112.686 kb on + strand at 112.686 kb on + strand at 112.687 kb on - strand at 112.687 kb on - strand at 112.843 kb on + strand at 112.870 kb on + strand at 112.871 kb on - strand at 112.871 kb on - strand at 112.903 kb on + strand at 112.903 kb on + strand at 112.903 kb on + strand at 112.904 kb on - strand at 112.904 kb on - strand at 112.904 kb on - strand at 112.904 kb on - strand at 112.904 kb on - strand at 112.912 kb on + strand at 112.913 kb on - strand at 112.913 kb on - strand at 113.038 kb on + strand at 113.038 kb on + strand at 113.038 kb on + strand at 113.038 kb on + strand at 113.039 kb on - strand at 113.133 kb on + strand, within Pf1N1B4_5379 at 113.156 kb on + strand, within Pf1N1B4_5379 at 113.360 kb on + strand at 113.360 kb on + strand at 113.361 kb on - strand at 113.372 kb on + strand at 113.407 kb on + strand at 113.428 kb on - strand at 113.629 kb on + strand, within Pf1N1B4_5380 at 113.629 kb on + strand, within Pf1N1B4_5380 at 113.629 kb on + strand, within Pf1N1B4_5380 at 113.629 kb on + strand at 113.630 kb on - strand, within Pf1N1B4_5380 at 113.632 kb on + strand, within Pf1N1B4_5380 at 113.632 kb on + strand, within Pf1N1B4_5380 at 113.633 kb on - strand, within Pf1N1B4_5380 at 113.633 kb on - strand, within Pf1N1B4_5380 at 113.659 kb on + strand, within Pf1N1B4_5380 at 113.659 kb on + strand, within Pf1N1B4_5380 at 113.659 kb on + strand, within Pf1N1B4_5380 at 113.659 kb on + strand, within Pf1N1B4_5380 at 113.660 kb on - strand at 113.682 kb on + strand, within Pf1N1B4_5380 at 113.721 kb on + strand, within Pf1N1B4_5380 at 113.758 kb on + strand, within Pf1N1B4_5380 at 113.944 kb on + strand at 113.944 kb on + strand at 113.944 kb on + strand at 113.944 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.013 kb on - strand at 114.013 kb on - strand at 114.013 kb on - strand at 114.035 kb on + strand at 114.101 kb on + strand at 114.109 kb on - strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.144 kb on - strand at 114.144 kb on - strand at 114.144 kb on - strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.148 kb on - strand at 114.148 kb on - strand at 114.148 kb on - strand at 114.148 kb on - strand at 114.163 kb on + strand at 114.163 kb on + strand at 114.163 kb on + strand at 114.164 kb on - strand at 114.181 kb on + strand at 114.181 kb on + strand at 114.281 kb on + strand, within Pf1N1B4_5381 at 114.286 kb on + strand, within Pf1N1B4_5381 at 114.286 kb on + strand, within Pf1N1B4_5381 at 114.320 kb on + strand, within Pf1N1B4_5381 at 114.321 kb on - strand, within Pf1N1B4_5381 at 114.343 kb on + strand at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 112,088 + Pf1N1B4_5378 0.84 -0.0 112,088 + Pf1N1B4_5378 0.84 +1.0 112,088 + Pf1N1B4_5378 0.84 -0.0 112,088 + Pf1N1B4_5378 0.84 -0.0 112,088 + Pf1N1B4_5378 0.84 -0.0 112,088 + -1.7 112,089 - Pf1N1B4_5378 0.84 -0.0 112,089 - Pf1N1B4_5378 0.84 -3.8 112,089 - Pf1N1B4_5378 0.84 -0.0 112,089 - Pf1N1B4_5378 0.84 -0.0 112,089 - Pf1N1B4_5378 0.84 -0.0 112,332 + -0.0 112,332 + -0.0 112,333 - -0.0 112,333 - -0.0 112,333 - -2.4 112,347 + -1.8 112,347 + -0.0 112,347 + -0.0 112,347 + -1.5 112,348 - -1.1 112,348 - -0.0 112,348 - -0.0 112,348 - -0.0 112,348 - -2.5 112,348 - -0.0 112,348 - -1.8 112,348 - -0.0 112,386 - -0.0 112,427 + -2.8 112,427 + -0.0 112,431 + -0.0 112,431 + -0.0 112,431 + -0.0 112,431 + -1.4 112,431 + -1.7 112,431 + -0.0 112,431 + -1.6 112,432 - -3.4 112,432 - -0.0 112,432 - -0.0 112,432 - -0.0 112,432 - -0.0 112,432 - -1.7 112,570 + -0.0 112,570 + -0.0 112,570 + -2.6 112,571 - -0.0 112,571 - -0.0 112,636 + -1.0 112,636 + -0.0 112,649 + -0.0 112,649 + -0.0 112,650 - -0.0 112,650 - -0.0 112,650 - -0.0 112,686 + -0.0 112,686 + -0.0 112,686 + -0.7 112,687 - -2.5 112,687 - -0.0 112,843 + -0.0 112,870 + -0.0 112,871 - -0.0 112,871 - +1.0 112,903 + -0.0 112,903 + -1.3 112,903 + -0.0 112,904 - -0.0 112,904 - -0.0 112,904 - -2.3 112,904 - -2.4 112,904 - -0.0 112,912 + -0.0 112,913 - -0.0 112,913 - -0.0 113,038 + -0.0 113,038 + -0.0 113,038 + -0.0 113,038 + -1.4 113,039 - -0.0 113,133 + Pf1N1B4_5379 0.29 -0.0 113,156 + Pf1N1B4_5379 0.36 -0.0 113,360 + -0.0 113,360 + -0.0 113,361 - -0.0 113,372 + -0.0 113,407 + -0.0 113,428 - -1.6 113,629 + Pf1N1B4_5380 0.33 -0.0 113,629 + Pf1N1B4_5380 0.33 -1.8 113,629 + Pf1N1B4_5380 0.33 -2.0 113,629 + -0.0 113,630 - Pf1N1B4_5380 0.33 -1.5 113,632 + Pf1N1B4_5380 0.34 -1.6 113,632 + Pf1N1B4_5380 0.34 -0.0 113,633 - Pf1N1B4_5380 0.34 -1.8 113,633 - Pf1N1B4_5380 0.34 -0.0 113,659 + Pf1N1B4_5380 0.40 -1.9 113,659 + Pf1N1B4_5380 0.40 -0.0 113,659 + Pf1N1B4_5380 0.40 -2.7 113,659 + Pf1N1B4_5380 0.40 -0.0 113,660 - -1.5 113,682 + Pf1N1B4_5380 0.45 -1.6 113,721 + Pf1N1B4_5380 0.54 -1.6 113,758 + Pf1N1B4_5380 0.63 -0.0 113,944 + -0.0 113,944 + -0.0 113,944 + -0.0 113,944 + -3.5 114,012 + -1.1 114,012 + -0.0 114,012 + -0.0 114,012 + -0.0 114,012 + -0.0 114,012 + -0.0 114,012 + -0.0 114,013 - -2.0 114,013 - -0.0 114,013 - -0.6 114,035 + -0.0 114,101 + -0.0 114,109 - -0.7 114,143 + -0.0 114,143 + -0.0 114,143 + -0.0 114,143 + -0.0 114,143 + -0.0 114,143 + -0.0 114,143 + -0.0 114,143 + -0.0 114,143 + -1.3 114,143 + -1.9 114,143 + -0.0 114,143 + -0.0 114,143 + -2.2 114,143 + -0.0 114,143 + -0.0 114,143 + -0.0 114,143 + -0.0 114,144 - -0.0 114,144 - -0.0 114,144 - -2.1 114,147 + -0.0 114,147 + -0.0 114,147 + -0.0 114,147 + -0.6 114,147 + -0.0 114,147 + -2.6 114,147 + -0.0 114,147 + -0.0 114,147 + -0.0 114,147 + -0.0 114,147 + -0.0 114,148 - -0.0 114,148 - -1.3 114,148 - -0.0 114,148 - -0.0 114,163 + -0.0 114,163 + -0.0 114,163 + -0.0 114,164 - -0.8 114,181 + -0.0 114,181 + -0.0 114,281 + Pf1N1B4_5381 0.35 -2.7 114,286 + Pf1N1B4_5381 0.37 -0.0 114,286 + Pf1N1B4_5381 0.37 -4.5 114,320 + Pf1N1B4_5381 0.49 -0.0 114,321 - Pf1N1B4_5381 0.49 -0.0 114,343 + -4.3 114,343 + Pf1N1B4_5381 0.57 -0.0 114,343 + Pf1N1B4_5381 0.57 -0.0 114,343 + Pf1N1B4_5381 0.57 -1.9 114,343 + Pf1N1B4_5381 0.57 -0.0 114,343 + Pf1N1B4_5381 0.57 -0.0 114,343 + Pf1N1B4_5381 0.57 -0.0 114,343 + Pf1N1B4_5381 0.57 -0.0 114,343 + Pf1N1B4_5381 0.57 +0.6
Or see this region's nucleotide sequence