Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5377 and Pf1N1B4_5378 are separated by 577 nucleotides Pf1N1B4_5378 and Pf1N1B4_5379 are separated by 756 nucleotides
Pf1N1B4_5377 - Uncharacterized domain COG3236 / GTP cyclohydrolase II (EC 3.5.4.25), at 110,017 to 110,511
_5377
Pf1N1B4_5378 - Phage protein, at 111,089 to 112,282
_5378
Pf1N1B4_5379 - hypothetical protein, at 113,039 to 113,362
_5379
Position (kb)
111
112
113 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 110.154 kb on - strand, within Pf1N1B4_5377 at 110.170 kb on + strand, within Pf1N1B4_5377 at 110.263 kb on - strand, within Pf1N1B4_5377 at 110.289 kb on - strand, within Pf1N1B4_5377 at 110.390 kb on + strand, within Pf1N1B4_5377 at 110.391 kb on - strand, within Pf1N1B4_5377 at 110.494 kb on - strand at 110.494 kb on - strand at 110.494 kb on - strand at 110.580 kb on + strand at 110.581 kb on - strand at 110.605 kb on + strand at 110.605 kb on + strand at 110.605 kb on + strand at 110.605 kb on + strand at 110.605 kb on + strand at 110.605 kb on + strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.609 kb on + strand at 110.609 kb on + strand at 110.609 kb on + strand at 110.609 kb on + strand at 110.610 kb on - strand at 110.610 kb on - strand at 110.653 kb on + strand at 110.653 kb on + strand at 110.653 kb on + strand at 110.654 kb on - strand at 110.654 kb on - strand at 110.689 kb on + strand at 110.690 kb on - strand at 110.697 kb on + strand at 110.697 kb on + strand at 110.697 kb on + strand at 110.698 kb on - strand at 110.698 kb on - strand at 110.698 kb on - strand at 110.698 kb on - strand at 111.110 kb on + strand at 111.110 kb on + strand at 111.111 kb on - strand at 111.111 kb on - strand at 111.111 kb on - strand at 111.111 kb on - strand at 111.111 kb on - strand at 111.536 kb on - strand at 111.536 kb on - strand at 111.625 kb on - strand, within Pf1N1B4_5378 at 112.088 kb on + strand, within Pf1N1B4_5378 at 112.088 kb on + strand, within Pf1N1B4_5378 at 112.088 kb on + strand, within Pf1N1B4_5378 at 112.088 kb on + strand, within Pf1N1B4_5378 at 112.088 kb on + strand, within Pf1N1B4_5378 at 112.088 kb on + strand at 112.089 kb on - strand, within Pf1N1B4_5378 at 112.089 kb on - strand, within Pf1N1B4_5378 at 112.089 kb on - strand, within Pf1N1B4_5378 at 112.089 kb on - strand, within Pf1N1B4_5378 at 112.089 kb on - strand, within Pf1N1B4_5378 at 112.332 kb on + strand at 112.332 kb on + strand at 112.333 kb on - strand at 112.333 kb on - strand at 112.333 kb on - strand at 112.347 kb on + strand at 112.347 kb on + strand at 112.347 kb on + strand at 112.347 kb on + strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.386 kb on - strand at 112.427 kb on + strand at 112.427 kb on + strand at 112.431 kb on + strand at 112.431 kb on + strand at 112.431 kb on + strand at 112.431 kb on + strand at 112.431 kb on + strand at 112.431 kb on + strand at 112.431 kb on + strand at 112.432 kb on - strand at 112.432 kb on - strand at 112.432 kb on - strand at 112.432 kb on - strand at 112.432 kb on - strand at 112.432 kb on - strand at 112.570 kb on + strand at 112.570 kb on + strand at 112.570 kb on + strand at 112.571 kb on - strand at 112.571 kb on - strand at 112.636 kb on + strand at 112.636 kb on + strand at 112.649 kb on + strand at 112.649 kb on + strand at 112.650 kb on - strand at 112.650 kb on - strand at 112.650 kb on - strand at 112.686 kb on + strand at 112.686 kb on + strand at 112.686 kb on + strand at 112.687 kb on - strand at 112.687 kb on - strand at 112.843 kb on + strand at 112.870 kb on + strand at 112.871 kb on - strand at 112.871 kb on - strand at 112.903 kb on + strand at 112.903 kb on + strand at 112.903 kb on + strand at 112.904 kb on - strand at 112.904 kb on - strand at 112.904 kb on - strand at 112.904 kb on - strand at 112.904 kb on - strand at 112.912 kb on + strand at 112.913 kb on - strand at 112.913 kb on - strand at 113.038 kb on + strand at 113.038 kb on + strand at 113.038 kb on + strand at 113.038 kb on + strand at 113.039 kb on - strand at 113.133 kb on + strand, within Pf1N1B4_5379 at 113.156 kb on + strand, within Pf1N1B4_5379
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 110,154 - Pf1N1B4_5377 0.28 -1.8 110,170 + Pf1N1B4_5377 0.31 -1.4 110,263 - Pf1N1B4_5377 0.50 -0.0 110,289 - Pf1N1B4_5377 0.55 -0.0 110,390 + Pf1N1B4_5377 0.75 -0.0 110,391 - Pf1N1B4_5377 0.76 -0.0 110,494 - -0.0 110,494 - -1.6 110,494 - -0.0 110,580 + -0.0 110,581 - -0.0 110,605 + -1.9 110,605 + -2.0 110,605 + -0.0 110,605 + -1.2 110,605 + -0.0 110,605 + -1.7 110,606 - -0.0 110,606 - -2.0 110,606 - -0.0 110,606 - -1.6 110,606 - -0.0 110,606 - -0.0 110,606 - -0.0 110,606 - -1.8 110,606 - -0.0 110,609 + -0.0 110,609 + -2.1 110,609 + -0.0 110,609 + -0.0 110,610 - -0.0 110,610 - -2.4 110,653 + -1.6 110,653 + -0.0 110,653 + -0.0 110,654 - -0.0 110,654 - -1.6 110,689 + -0.0 110,690 - -0.0 110,697 + -1.8 110,697 + -1.0 110,697 + -2.1 110,698 - -1.4 110,698 - -0.0 110,698 - -0.0 110,698 - -0.0 111,110 + -0.0 111,110 + -1.5 111,111 - -0.0 111,111 - -0.0 111,111 - -1.6 111,111 - -0.0 111,111 - -0.0 111,536 - -0.0 111,536 - -2.2 111,625 - Pf1N1B4_5378 0.45 -0.0 112,088 + Pf1N1B4_5378 0.84 -0.0 112,088 + Pf1N1B4_5378 0.84 +1.0 112,088 + Pf1N1B4_5378 0.84 -0.0 112,088 + Pf1N1B4_5378 0.84 -0.0 112,088 + Pf1N1B4_5378 0.84 -0.0 112,088 + -1.7 112,089 - Pf1N1B4_5378 0.84 -0.0 112,089 - Pf1N1B4_5378 0.84 -3.8 112,089 - Pf1N1B4_5378 0.84 -0.0 112,089 - Pf1N1B4_5378 0.84 -0.0 112,089 - Pf1N1B4_5378 0.84 -0.0 112,332 + -0.0 112,332 + -0.0 112,333 - -0.0 112,333 - -0.0 112,333 - -2.4 112,347 + -1.8 112,347 + -0.0 112,347 + -0.0 112,347 + -1.5 112,348 - -1.1 112,348 - -0.0 112,348 - -0.0 112,348 - -0.0 112,348 - -2.5 112,348 - -0.0 112,348 - -1.8 112,348 - -0.0 112,386 - -0.0 112,427 + -2.8 112,427 + -0.0 112,431 + -0.0 112,431 + -0.0 112,431 + -0.0 112,431 + -1.4 112,431 + -1.7 112,431 + -0.0 112,431 + -1.6 112,432 - -3.4 112,432 - -0.0 112,432 - -0.0 112,432 - -0.0 112,432 - -0.0 112,432 - -1.7 112,570 + -0.0 112,570 + -0.0 112,570 + -2.6 112,571 - -0.0 112,571 - -0.0 112,636 + -1.0 112,636 + -0.0 112,649 + -0.0 112,649 + -0.0 112,650 - -0.0 112,650 - -0.0 112,650 - -0.0 112,686 + -0.0 112,686 + -0.0 112,686 + -0.7 112,687 - -2.5 112,687 - -0.0 112,843 + -0.0 112,870 + -0.0 112,871 - -0.0 112,871 - +1.0 112,903 + -0.0 112,903 + -1.3 112,903 + -0.0 112,904 - -0.0 112,904 - -0.0 112,904 - -2.3 112,904 - -2.4 112,904 - -0.0 112,912 + -0.0 112,913 - -0.0 112,913 - -0.0 113,038 + -0.0 113,038 + -0.0 113,038 + -0.0 113,038 + -1.4 113,039 - -0.0 113,133 + Pf1N1B4_5379 0.29 -0.0 113,156 + Pf1N1B4_5379 0.36 -0.0
Or see this region's nucleotide sequence